Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,925,4300TG24.1% 244.1 / 20.7 116N1876H (AAC→CAC) EO946_RS09730non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base T (12/76);  new base G (28/0);  total (40/76)
Fisher's exact test for biased strand distribution p-value = 9.31e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 123 total aligned reads displayed.

TTATATTGCTGATCCGCTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCC  >  NZ_CP034943/1925292‑1925563
                                                                                                                                          |                                                                                                                                     
ttATATTGCTGATCCGCTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGttt                                                                                                                                      <  1:1133079/140‑1 (MQ=255)
ttATATTGCTGATCCGCTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGttt                                                                                                                                      <  1:324621/140‑1 (MQ=255)
ttATATTGCTGATCCGCTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGttt                                                                                                                                      <  1:324610/140‑1 (MQ=255)
ttATATTGCTGATCCGCTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGttt                                                                                                                                      <  1:2475970/140‑1 (MQ=255)
 tataTTGCTGATCCGCTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTa                                                                                                                                     <  2:1082698/140‑1 (MQ=255)
  atatTGCTGATCCGCTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTaa                                                                                                                                    <  2:1488234/140‑1 (MQ=255)
   tatTGCTGATCCGCTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGCGTCGTTTAat                                                                                                                                   <  2:1693165/140‑1 (MQ=255)
   tatTGCTGATCCGCTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAat                                                                                                                                   <  2:2142499/140‑1 (MQ=255)
   tatTGCTGATCCGCTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAat                                                                                                                                   <  2:1835972/140‑1 (MQ=255)
   tatTGCTGATCCGCTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAat                                                                                                                                   <  1:217097/140‑1 (MQ=255)
   tatTGCTGATCCGCTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAat                                                                                                                                   <  2:1181402/140‑1 (MQ=255)
   tatTGCTGATCCGCTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAat                                                                                                                                   <  2:1105558/140‑1 (MQ=255)
   tatTGCTGATCCGCTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAat                                                                                                                                   <  1:294595/140‑1 (MQ=255)
   tagggCTGATCCGCTCAAGATCAGAGCGCCCCTTGTCCGCAGAATTTTCATCATCCAATAGACCGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCGGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAat                                                                                                                                   <  2:2225543/136‑1 (MQ=255)
      tGCTGATCCGCTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTGTTatatt                                                                                                                                >  1:2825701/1‑139 (MQ=255)
        cTGATCCGCTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAatatata                                                                                                                              <  1:1726429/140‑1 (MQ=255)
        cTGATCCGCTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTGTAattaata                                                                                                                              >  2:909133/1‑135 (MQ=255)
                cTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATTGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAATACAATTTCTGTTTTAGAGTAGTGTTAAATATATTTTAttg                                                                                                                      >  2:1309262/1‑139 (MQ=255)
                cTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTCGAGTCGTGTAAAATAAAATGTAtac                                                                                                                      >  2:66592/1‑138 (MQ=255)
                cTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATAGTaata                                                                                                                      >  2:2149983/1‑137 (MQ=255)
                cTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTGTa                                                                                                                                     >  2:3389903/1‑125 (MQ=255)
                cTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTGTAATATATATTTTAtta                                                                                                                      >  2:933081/1‑139 (MQ=255)
                cTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTGTAATATAAAATTTAtta                                                                                                                      >  1:1864778/1‑139 (MQ=255)
                 tCAAGATTCGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGAGGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATCTATTGTATTCg                                                                                                                     <  2:2494603/140‑1 (MQ=255)
                 tCAAGATTAGTGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                     <  1:2149983/140‑1 (MQ=255)
                 tCAAGATTAGAGCGCTCCTTGTTATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                     <  2:2049248/140‑1 (MQ=255)
                 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                     <  2:1893042/140‑1 (MQ=255)
                 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                     <  2:1522569/140‑1 (MQ=255)
                 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                     <  2:1205260/140‑1 (MQ=255)
                 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                     <  2:64862/140‑1 (MQ=255)
                 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                     <  2:1181283/140‑1 (MQ=255)
                 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                     <  1:215518/140‑1 (MQ=255)
                 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                     <  2:1874430/140‑1 (MQ=255)
                 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                     <  2:3387818/140‑1 (MQ=255)
                 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                     <  1:1948049/140‑1 (MQ=255)
                 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                     <  2:3298150/140‑1 (MQ=255)
                 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                     <  2:2707957/140‑1 (MQ=255)
                 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                     <  1:2616605/140‑1 (MQ=255)
                 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAACATATATTGTATTCg                                                                                                                     <  2:1080773/140‑1 (MQ=255)
                 gccagcttAGAGCGCTCCGCGTGATACCCAGGTTCATCATCCCATAGAACGAGCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTGAGAGTCGTTTAATATATATTGTATTCg                                                                                                                     <  2:1957099/134‑1 (MQ=255)
                      aTTAGAGCTCTCCTTGTGATAAGAATTTTCATCATCCATTTGAACGATCGTGCCTTCATGGGCCTATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCgctgct                                                                                                                <  2:1605024/140‑1 (MQ=255)
                      aTTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCgctgct                                                                                                                <  2:1052221/140‑1 (MQ=255)
                      aTTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCgctgct                                                                                                                <  2:219456/140‑1 (MQ=255)
                      aTTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCgctgct                                                                                                                <  2:2220873/140‑1 (MQ=255)
                      aTTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCgctgct                                                                                                                <  1:1117547/140‑1 (MQ=255)
                        tAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTTAAAGAAATTGTATTAgctggagg                                                                                                              >  2:1724841/1‑136 (MQ=255)
                          gagCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTGATATATATTTTATTAGCTGCTCTgg                                                                                                            >  2:277489/1‑140 (MQ=255)
                           agCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATCTATAATTTATTTtctgctggtga                                                                                                           >  1:1798700/1‑135 (MQ=255)
                           agCGCTCCTTGTGATAAGAATTGTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTACAGTCGTTTAATATATAATGTATTCGCTGCTCTGGa                                                                                                           <  1:3126239/140‑1 (MQ=255)
                               cTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGttt                                                                                                                                      >  1:714428/1‑109 (MQ=255)
                               cTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGttt                                                                                                                                      <  2:714428/109‑1 (MQ=255)
                               cTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTTATATATAATTTATTAGCTGCTCTTGATatt                                                                                                       >  2:1092/1‑138 (MQ=255)
                               cTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAAGATAAATTGTATTAGCGGCTCGGGTTAGt                                                                                                       >  1:2227391/1‑140 (MQ=255)
                               cTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTGTTATATAAATTTTATTAGCTTCGGTGGCTAGt                                                                                                       >  2:907194/1‑140 (MQ=255)
                               cTCCTTGTGATAAGAATTTTCATCAACCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTGTAAAATAAAAATTATTAGCTGCTCTTGATatt                                                                                                       >  2:1722016/1‑138 (MQ=255)
                                tCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTTATAAATATTGTATTTGCGGCTCTGGATatta                                                                                                      >  2:90720/1‑137 (MQ=255)
                                tCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTc                                                                                                      <  2:2857370/140‑1 (MQ=255)
                                tCCTGGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTGTAATATAAAGTGTTTGAGGTGCTGTGGATAGtg                                                                                                      >  2:193433/1‑139 (MQ=255)
                                   ttGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCgg                                                                                                   <  1:1149100/140‑1 (MQ=255)
                                       gATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCa                                                                                               <  2:290794/140‑1 (MQ=255)
                                       gATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCa                                                                                               <  2:2156240/140‑1 (MQ=255)
                                         tAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAAt                                                                                             <  2:3024056/140‑1 (MQ=255)
                                          aaGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATa                                                                                            <  1:1612762/140‑1 (MQ=255)
                                          aaGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATa                                                                                            <  1:876366/140‑1 (MQ=255)
                                          aaGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATa                                                                                            <  1:495707/140‑1 (MQ=255)
                                                          cAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGcgccgc                                                                            <  2:1543177/140‑1 (MQ=255)
                                                          cAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGcgccgc                                                                            <  2:1868656/140‑1 (MQ=255)
                                                           aaTAGAACGATCGTGAGTTCATGGGCCAATCTCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCgccgcc                                                                           <  1:1895585/140‑1 (MQ=255)
                                                                  cGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGttt                                                                                                                                      <  1:2444022/74‑1 (MQ=255)
                                                                  cGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTGTTATATATATTGTATTAGGTGCTCTGGATAGTGCGGATCAATATGGCCCTAGGCGCCGCCGGCtgtt                                                                    >  2:1972440/1‑140 (MQ=255)
                                                                  cGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTGTAATATATATTGTATTGGCGGCGCTGGATAGTCCGGATAAATATGCAAGGAGGCGCCCCCCGCgttt                                                                    >  2:771287/1‑140 (MQ=255)
                                                                  cGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTGGTATATATATTTTTTTTGCTGGGGTTGATAGTGCGGAACAAAAAGCCCCTATGCCCCCCCGGCtttt                                                                    >  2:1412577/1‑140 (MQ=255)
                                                                    aTCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTGTAATATATATTTTATTAGCTGCTCTGGCTAGTGCGGGTCAATAGGCCCGTAAGCGCCGCCCGCGATTaa                                                                  >  2:1674071/1‑140 (MQ=255)
                                                                     tCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTGTTATAAATATTTTTTTAGCTGCTCGGGATATTACGGATGAAAATGCACCGAGGCGCCCCCGGCGTTTAAt                                                                 >  1:3383205/1‑140 (MQ=255)
                                                                        tGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTTATATATAATGTATTTGCTGCTATTGTTAGTACGGATGAATAGGAACGTTAGCGCCCCCGGCTTTTAATaag                                                              >  2:2242937/1‑138 (MQ=255)
                                                                        tGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTGTAATATATATTGTATTAGCTGCTCTGGCTATTCCGGTACAAAATGCACCGAGGCGCCGCCCGCGTTTAATaaa                                                              >  2:348955/1‑138 (MQ=255)
                                                                          ccTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGc                                                            <  1:3346807/140‑1 (MQ=255)
                                                                          ccTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGc                                                            <  1:3138629/140‑1 (MQ=255)
                                                                          ccTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTGTTATATATATTTTTTTTGGTGGTCTGGGTATTGCCGGTAAATAGGCCCGGAGGCGCCCCCCGGGGTTAATAAGGc                                                            >  1:2757302/1‑140 (MQ=255)
                                                                               aTGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGaa                                                       <  2:1882551/140‑1 (MQ=255)
                                                                                   gCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTcc                                                   <  2:3276275/140‑1 (MQ=255)
                                                                                       aTCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTGTTATTGATAATTTATTAGCTGAGCGGGATAGTCCCGATCAAAAAGCACCTAGTCGCCGCCGCCTTTTAATAAGGCCCGAATTAAcaca                                               >  2:1932591/1‑140 (MQ=255)
                                                                                       aTCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTGTTATATATATTGTATTAGCTGGTGAGGATATTCCGGGTCAAAATGCACGGAGTGGCCGCCCGCTTGTTATAAGGCCACAATTGAcaca                                               >  2:1810067/1‑140 (MQ=255)
                                                                                         cGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAAc                                             <  1:1674071/140‑1 (MQ=255)
                                                                                              ctgAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGtt                                        <  1:1217003/140‑1 (MQ=255)
                                                                                                     tGGCTCTGTGTCAATACACTTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCt                                 <  1:907194/140‑1 (MQ=255)
                                                                                                       gCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCtgt                               <  2:2519624/140‑1 (MQ=255)
                                                                                                        ctctGTGTCAAGACAATTTCTGTTTTAGAGTCGTGTTATATATATTTTTTTGGGGGGTCTGGGTAGTGCGGATCAAAAGGCCCCGACGCGCCCCCGGCTTTTAAAAAGGCCCGAATTACCACAACCAATTCGAATCtgtg                              >  2:462917/1‑140 (MQ=255)
                                                                                                            gtgtCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTAGTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCg                          <  2:1843849/140‑1 (MQ=255)
                                                                                                              gtCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCt                        <  1:2373668/140‑1 (MQ=255)
                                                                                                                   gACAATTTCTGTTTTAGAGTCGTTTTATATATATTTTATTAGCTGCTCTTGGTATTGCGGATAAATATTGACCTAGGCGCCGCCGGCGTTTAATAAAGCCAGAAATACCACAACCACATAGCTGATGTGGTGGCggatga                   >  2:3075386/1‑140 (MQ=255)
                                                                                                                       aTTTCTGTTTTAGAGTCGTGTTATATATATTTTTTTTGCTGCTCTGGATATTCCGGGTAAAAAGGCACGGAGGCGCCCCCCGCGTTTTATACCGCCAGAAATCCCACAACCAGTTCGATGCGGTGGTGGCTGATGATAGc               >  2:2509830/1‑140 (MQ=255)
                                                                                                                       aTTTCTGTTTTAGAGTCGTGTAATATATATTTTATTAGCTGCTCTGGGTAGTGCGGATCAAAATGCACCTATGCGCCGCCCGCTTTTAATAAGGCCACAATTGCCACAAACACTTCGATTCGGTTGTGGCGGTTGATAGc               >  2:2520727/1‑140 (MQ=255)
                                                                                                                       aTTTCTGTTTTAGAGTCGTGTAATATATATTGTATTAGCTGCTCTGGATATTACGGATCAATAGGCACCTACGCCCCGCCGGCTTTTAATAAGGCCACAATTACCACAACACCGTCGCTGCGGTGGTCGCTGATGCTacg               >  2:2003473/1‑138 (MQ=255)
                                                                                                                        tttCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCt              <  1:1724841/140‑1 (MQ=255)
                                                                                                                              ttttAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGtt        <  2:1758901/140‑1 (MQ=255)
                                                                                                                              ttttAGAGTAGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCTGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGtt        <  1:1810067/140‑1 (MQ=255)
                                                                                                                                   gagTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTc   <  1:1518614/140‑1 (MQ=255)
                                                                                                                                    agTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTcc  <  2:418346/140‑1 (MQ=255)
                                                                                                                                    agTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTcc  <  2:2446371/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                     
TTATATTGCTGATCCGCTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCC  >  NZ_CP034943/1925292‑1925563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: