Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,285,7130GA38.8% 164.1 / 160.1 136L230L (CTA→TTA) EO946_RS16995IS3 family transposase
Reads supporting (aligned to +/- strand):  ref base G (38/44);  new base A (32/20);  total (72/64)
Fisher's exact test for biased strand distribution p-value = 1.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 136 total aligned reads displayed.

ATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCATTC  >  NZ_CP034943/3285580‑3285786
                                                                                                                                     |                                                                         
aTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTc                                                                     >  2:3349192/1‑140 (MQ=40)
aTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTc                                                                     >  2:1916566/1‑140 (MQ=40)
 tGTCTAAGACAAATTCAGTGTCCTGCTTATCCCCAATATTAAAAGCAATAACTTCTCCATTGTATAAATATAATATACAGTAAAGGTAAAATTGTTTATGTACATAAGGAAAATAAGTGATGTACTTTAATAATTTATCa                                                                    >  2:2313544/1‑140 (MQ=2)
 tGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTCTAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGGGATGTCCGTGACTAATTTTTCa                                                                    >  2:3225967/1‑140 (MQ=11)
 tGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCa                                                                    >  2:1875446/1‑140 (MQ=21)
 tGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCa                                                                    >  2:1570132/1‑140 (MQ=21)
 tGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCa                                                                    >  1:2177442/1‑140 (MQ=21)
 tGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCa                                                                    >  2:2150155/1‑140 (MQ=21)
   tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAag                                                                  >  2:769066/1‑140 (MQ=40)
   tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAag                                                                  <  2:3002213/140‑1 (MQ=21)
     tAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAagag                                                                <  2:209015/140‑1 (MQ=21)
       aGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAggg                                                              >  2:1744353/1‑140 (MQ=40)
           aaaGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGAt                                                          <  1:41097/140‑1 (MQ=40)
           aaaGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGAt                                                          <  1:3011404/140‑1 (MQ=21)
           aaaGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGAt                                                          <  2:3006981/140‑1 (MQ=21)
           aaaGTCTGTGTCCTGATTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGAt                                                          <  1:2508798/140‑1 (MQ=255)
             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAATAGGGCGATCa                                                        >  2:407673/1‑140 (MQ=255)
             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                        >  1:1940524/1‑140 (MQ=40)
             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                        >  1:2709452/1‑140 (MQ=40)
             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                        >  2:726946/1‑140 (MQ=40)
             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                        >  2:2326121/1‑140 (MQ=40)
             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                        >  1:2169353/1‑140 (MQ=40)
             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                        >  1:233213/1‑140 (MQ=40)
             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                        >  1:2933255/1‑140 (MQ=21)
             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                        >  2:1146078/1‑140 (MQ=21)
             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                        >  1:3215979/1‑140 (MQ=21)
             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                        >  1:1182563/1‑140 (MQ=21)
             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                        >  1:810956/1‑140 (MQ=21)
             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTGACTAATTTTTCAAGAGGGCGATCa                                                        >  1:1522937/1‑140 (MQ=255)
                   tgtCCTGCTTATCCCCACTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGa                                                  <  1:1144884/140‑1 (MQ=255)
                         gCTTATCCCCAATCGTAACAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTACATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTc                                            <  1:2014627/140‑1 (MQ=255)
                         gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTCTACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATCTGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTc                                            <  1:422767/140‑1 (MQ=255)
                         gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTc                                            <  1:3309427/140‑1 (MQ=40)
                         gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTc                                            >  2:816435/1‑140 (MQ=21)
                         gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATAAGACTGAAAGTTc                                            >  2:2942118/1‑140 (MQ=255)
                           ttATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCg                                          <  1:1244003/140‑1 (MQ=40)
                             aTCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATATATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGAt                                        >  2:3009151/1‑140 (MQ=25)
                             aTCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGag                                        >  2:585704/1‑139 (MQ=38)
                             aTCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGAt                                        >  1:231100/1‑140 (MQ=18)
                              tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                       <  1:56550/140‑1 (MQ=37)
                              tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                       <  1:2273695/140‑1 (MQ=37)
                              tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                       <  2:2218155/140‑1 (MQ=37)
                              tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                       <  1:1368411/140‑1 (MQ=37)
                              tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                       <  2:2868534/140‑1 (MQ=37)
                              tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                       <  2:2676375/140‑1 (MQ=17)
                              tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                       <  1:2953531/140‑1 (MQ=17)
                              tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                       <  1:1146078/140‑1 (MQ=17)
                              tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                       <  2:781809/140‑1 (MQ=17)
                              tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                       <  1:1601424/140‑1 (MQ=17)
                              tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                       <  1:2055726/140‑1 (MQ=17)
                              tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                       <  2:3391687/140‑1 (MQ=17)
                              tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                       <  1:2640334/140‑1 (MQ=17)
                               ccccAATCGTAAAAGCAATCACTTCTCCATTGTCTAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATcc                                      >  2:1679206/1‑140 (MQ=9)
                               ccccAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATcc                                      >  1:2474734/1‑140 (MQ=37)
                               ccccAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                       >  2:2990888/1‑139 (MQ=37)
                               ccccAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCg                                          >  2:1123465/1‑136 (MQ=14)
                               ccccAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATcc                                      >  2:939801/1‑140 (MQ=14)
                               ccccAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATcc                                      >  2:1255579/1‑140 (MQ=14)
                               ccccAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                       <  2:1827031/139‑1 (MQ=14)
                               ccccAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                       >  1:1827031/1‑139 (MQ=14)
                               ccccAAACGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATcc                                      >  2:600795/1‑140 (MQ=9)
                                cccAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCa                                     <  2:3341818/140‑1 (MQ=35)
                                cccAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCa                                     <  1:680601/140‑1 (MQ=35)
                                cccAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCa                                     <  2:3270668/140‑1 (MQ=11)
                                  cAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAat                                   <  1:200770/140‑1 (MQ=34)
                                   aaTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAata                                  >  2:1977845/1‑140 (MQ=34)
                                    aTCGTAAAAGCAATCCCTTCTCCATTGTATACATCTAATATACTGGAAAGGTCCAAGTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGCAAGTTCCGATCCAatag                                 >  1:1105692/1‑139 (MQ=255)
                                    aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAatat                                 >  2:1592312/1‑140 (MQ=34)
                                    aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAatat                                 >  2:3036539/1‑140 (MQ=34)
                                        tAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATAtatta                             <  2:438055/140‑1 (MQ=32)
                                        tAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATAtatta                             <  1:3005276/140‑1 (MQ=32)
                                         aaaaGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTATTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATAtattat                            >  2:747197/1‑140 (MQ=17)
                                              cAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGAcc                       <  1:110470/140‑1 (MQ=32)
                                              cAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGAcc                       <  2:2649990/140‑1 (MQ=32)
                                               aaacccTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGGTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGGTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                      <  1:2740452/135‑1 (MQ=255)
                                               aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                      <  2:2453103/140‑1 (MQ=32)
                                               aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                      <  2:879156/140‑1 (MQ=32)
                                               aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                      <  2:837345/140‑1 (MQ=32)
                                               aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                      <  1:119615/140‑1 (MQ=32)
                                               aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                      <  1:1303700/140‑1 (MQ=32)
                                               aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                      <  1:67247/140‑1 (MQ=32)
                                               aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                      <  1:1744353/140‑1 (MQ=32)
                                               aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                      >  2:3377435/1‑140 (MQ=32)
                                               aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                      <  1:1977845/140‑1 (MQ=32)
                                               aaTAACTTCTACATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGATGTCTATCAGACTGAAAGTTCCGATCAATTATATTATCgtgta                      <  1:2770655/140‑5 (MQ=11)
                                                aTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                      <  1:463231/139‑1 (MQ=32)
                                                aTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCAc                     >  2:2770655/1‑140 (MQ=32)
                                                    cTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCa                 <  2:2745329/140‑1 (MQ=32)
                                                       ctcCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                       >  2:102169/1‑115 (MQ=32)
                                                       ctcCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                       >  2:1290066/1‑115 (MQ=32)
                                                       ctcCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                       <  1:102169/115‑1 (MQ=32)
                                                       ctcCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCatat              >  1:2201695/1‑140 (MQ=32)
                                                       ctcCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGAAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATAAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCatat              >  1:1280027/1‑140 (MQ=17)
                                                         ccATTGTATACATCTAAGATCCTGGACAGCTACACTTGTTTCTGTCCATAAGGCAAATATGCGATGTCCGTTACTAGTTTTTCACGAGGGCGATCAGACTGAAAGGTCCGATCCAATATATTATCGACCACGGCATATgg            <  1:1705297/140‑1 (MQ=11)
                                                                 taCATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGtttt                                                                       <  1:823601/73‑1 (MQ=34)
                                                                 taCATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGtttt                                                                       >  2:823601/1‑73 (MQ=34)
                                                                 taCATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAat                                   >  1:1335525/1‑109 (MQ=32)
                                                                 taCATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAat                                   <  2:1335525/109‑1 (MQ=32)
                                                                 taCATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCag    >  1:3379914/1‑139 (MQ=17)
                                                                   cATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCAttc  >  1:1986600/1‑140 (MQ=11)
                                                                                                                                     |                                                                         
ATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCATTC  >  NZ_CP034943/3285580‑3285786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: