Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP034943 452,125 T→A K146I (AAA→ATA)  EO946_RS02320 ← dienelactone hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943452,1250TA97.7% 762.8 / ‑0.0 222K146I (AAA→ATA) EO946_RS02320dienelactone hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base T (1/4);  new base A (93/123);  total (95/127)
Fisher's exact test for biased strand distribution p-value = 3.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 225 total aligned reads displayed.

TTTCTGCCTCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCAT  >  NZ_CP034943/451988‑452261
                                                                                                                                         |                                                                                                                                         
tttCTGCCTCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGtatt                                                                                                                                         <  1:2413354/140‑1 (MQ=255)
     gCCTCGTTTTTGATGATGGAAAGCGTTTTTAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGAt                                                                                                                                    <  2:1129709/140‑1 (MQ=255)
     gCCTCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGAt                                                                                                                                    <  2:1057240/140‑1 (MQ=255)
        tCGTGTTTGATGATGGAAAGCCTGTTGAAGAAGAGTGGGCTGTCAAAATATACCGCCTCGGGATTGGCAAAGCCATGAAGAGAATCAAAATGAGAAATCTCCACTTTGCGATGCTGTGGTTGCCTCAGTCTGTTGATAAg                                                                                                                                 >  2:3077458/1‑140 (MQ=255)
             tttGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCg                                                                                                                            <  1:774822/140‑1 (MQ=255)
              ttGATTATGGAATGAGTTTTGAAGAAGTGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGc                                                                                                                           <  1:3100769/140‑1 (MQ=255)
                          cgctttttGAAGACGCGTGGCCTGACAAAATAAACCGACTCGGGATTGGCAAAGCAATGAAGAGCATCAAATTGATAAAGCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAacga                                                                                                               <  1:264761/136‑1 (MQ=255)
                          aGCTTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAacga                                                                                                               <  2:3246130/140‑1 (MQ=255)
                          aGCGTTTTGAAGACTCGTGGCGTGAAAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAacga                                                                                                               <  2:912175/140‑1 (MQ=255)
                             gTTTTGACGAAGAGTGGCCGGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACgg                                                                                                            <  2:2723801/140‑1 (MQ=255)
                                      aagaGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAacga                                                                                                               <  2:1438030/128‑1 (MQ=255)
                                      aagaGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGa                                                                                                   >  2:154308/1‑140 (MQ=255)
                                      aagaGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGa                                                                                                   >  2:811436/1‑140 (MQ=255)
                                          gTGGCCGGTAAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAggg                                                                                               <  1:985956/140‑1 (MQ=255)
                                           tGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGa                                                                                             >  2:1661808/1‑140 (MQ=255)
                                             gCCTTTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                           <  1:300135/140‑1 (MQ=255)
                                             gCCTTTAAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATGGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                           <  1:3136718/140‑1 (MQ=255)
                                             gCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                           <  2:1474734/140‑1 (MQ=255)
                                             gCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                           <  1:111768/140‑1 (MQ=255)
                                             gCCTGTAAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                           <  2:1304948/140‑1 (MQ=255)
                                                 gTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                           <  2:642024/136‑1 (MQ=255)
                                                 gTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                           >  1:642024/1‑136 (MQ=255)
                                                  tCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACaa                                                                                      >  1:2403823/1‑140 (MQ=255)
                                                  tCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACaa                                                                                      >  2:1207283/1‑140 (MQ=255)
                                                  tCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACAGCAACATCAAACGACGGTTCATATGAAGGGAAAAACac                                                                                      >  2:1609210/1‑139 (MQ=255)
                                                  tCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGAGAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAATAACAGCAACATCAAACGACGGTTCATATGAAGGGAAAAACaa                                                                                      >  1:1743753/1‑140 (MQ=255)
                                                       aTAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGa                                                                                 <  2:3091249/140‑1 (MQ=255)
                                                       aTAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGa                                                                                 <  1:1290502/140‑1 (MQ=255)
                                                       aTAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGa                                                                                 <  2:1351865/140‑1 (MQ=255)
                                                            ccGACTGGGGATTGGCAAACCCATGAAGAGCATCAAATTGATAAATCTCCAAATGGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCggg                                                                            <  2:3177039/140‑1 (MQ=255)
                                                            ccGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCggg                                                                            >  1:115316/1‑140 (MQ=255)
                                                               actggtgATTGGCAAAGCCATGAAGAGCATAAAATTGATAAATCTCCAATTTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAg                                                                         <  1:2757902/134‑1 (MQ=255)
                                                                cTCGGGATTTGCAAAGCCATGAAGAGCATCAAATTCATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGa                                                                        >  1:2352605/1‑140 (MQ=255)
                                                                cTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGa                                                                        >  1:2711581/1‑140 (MQ=255)
                                                                    ggATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAAGAGCACCGGGCAGACTgg                                                                    >  1:1298290/1‑140 (MQ=255)
                                                                     gATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGc                                                                   <  2:1039127/140‑1 (MQ=255)
                                                                     gATTGGCAAAGCAATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGc                                                                   <  1:3060321/140‑1 (MQ=255)
                                                                     gATTGGAAAAGCCATGAAGAGAATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGc                                                                   <  1:1368576/140‑1 (MQ=255)
                                                                       ttGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCggg                                                                            <  2:1486829/129‑1 (MQ=255)
                                                                       ttGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCggg                                                                            >  1:1486829/1‑129 (MQ=255)
                                                                          gaaaaGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAACCAATAGGACCGGGCAGACTGGCACGtg                                                              <  2:2486448/138‑1 (MQ=255)
                                                                          gaaaaGCCATGAAGAGCATAAAATTGATAAATCTCAAAATTGGTATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGtg                                                              <  1:2726551/138‑1 (MQ=255)
                                                                          gCAAAGCCATGAAGAGCATCAAATGGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGtg                                                              <  1:1695190/140‑1 (MQ=255)
                                                                          gCAAAGCAATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGtg                                                              <  1:338390/140‑1 (MQ=255)
                                                                            aaaCCCATGAAGAGCATCAAATTTATAAATCTACAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGtgat                                                            <  2:1743753/140‑1 (MQ=255)
                                                                               gCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCAc                                                                 <  2:2210564/132‑1 (MQ=255)
                                                                               gCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCAc                                                                 >  1:2210564/1‑132 (MQ=255)
                                                                               gCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGAGGaa                                                         >  1:3198244/1‑140 (MQ=255)
                                                                                    gAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGaa                                                    >  1:2550202/1‑140 (MQ=255)
                                                                                    gAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGaa                                                    >  1:899993/1‑140 (MQ=255)
                                                                                      tgagCATCAAATTGATAACTCTCCAACTGGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGACCCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATc                                                  <  2:2819187/139‑1 (MQ=255)
                                                                                       gagCATCAATTTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCATTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATcg                                                 <  2:1989475/140‑1 (MQ=255)
                                                                                         gCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATcgcg                                               <  1:1372361/140‑1 (MQ=255)
                                                                                         gCATCAAATTGATAAATCACCAATTTGGGATGCTGTTGTTCCCTAAGTATTTTGATAAGAACCCCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGAAGACTGGCACGTGATGAAGAGAATcgcg                                               <  1:2425672/140‑1 (MQ=255)
                                                                                         ccATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATcgcg                                               <  2:566046/139‑1 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGTAGGGACCTACAATCGGACCGGGCCGACTGGCACGTGATGAAGAGACTCGCGt                                              >  1:3097972/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                              >  1:7430/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                              >  1:3153291/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                              >  1:2702656/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                              >  1:2092884/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                              >  1:178960/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                              >  1:1452337/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                              >  1:716744/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                              >  1:2889105/1‑140 (MQ=255)
                                                                                           aTCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAAACAAAAGTACCGGGCCGACTGGAACTTGTTCAAATGAATCCCGt                                              >  2:2291206/1‑140 (MQ=255)
                                                                                                  tGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGa                                      >  2:2069478/1‑140 (MQ=255)
                                                                                                      aaaTCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCg                                  <  2:454854/140‑1 (MQ=255)
                                                                                                      aaaTCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCg                                  <  2:748148/140‑1 (MQ=255)
                                                                                                                    ggATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGc                    >  1:1523968/1‑140 (MQ=255)
                                                                                                                     gATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCt                   >  1:545198/1‑140 (MQ=255)
                                                                                                                     gATGCTCTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCt                   <  2:2362302/140‑1 (MQ=255)
                                                                                                                        gCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCg                <  1:2593846/140‑1 (MQ=255)
                                                                                                                         cTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGACTCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGc               >  1:2973649/1‑140 (MQ=255)
                                                                                                                          tgttgtTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              <  2:684456/140‑1 (MQ=255)
                                                                                                                          tgttgtTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              <  2:1621228/140‑1 (MQ=255)
                                                                                                                          tgttgtTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              >  1:2084367/1‑140 (MQ=255)
                                                                                                                          tgttgtTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              <  2:1973391/140‑1 (MQ=255)
                                                                                                                          tgttgtTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              <  2:2084367/140‑1 (MQ=255)
                                                                                                                          tgttgtTCCCTCAGTAGTTTGATAAGACCCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              <  1:1607516/140‑1 (MQ=255)
                                                                                                                           gttgttCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTc             <  2:205197/140‑1 (MQ=255)
                                                                                                                             tgttCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCAc           <  2:2849826/140‑1 (MQ=255)
                                                                                                                                tCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              >  1:2266377/1‑134 (MQ=255)
                                                                                                                                tCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              <  2:3271532/134‑1 (MQ=255)
                                                                                                                                tCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              >  1:3271532/1‑134 (MQ=255)
                                                                                                                                tCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTc        <  2:772893/140‑1 (MQ=255)
                                                                                                                                tCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTc        <  2:2707379/140‑1 (MQ=255)
                                                                                                                                 cccTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCa       >  2:3342457/1‑140 (MQ=255)
                                                                                                                                 cccTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCa       >  2:2642165/1‑140 (MQ=255)
                                                                                                                                    tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAATACAATAGGAGCGGGCAGTCTGGCACGTGATGAAGAGACTCGCGTATCCGGGACCCGTAAGACCAAACAGCTGCGCTCTCCTCAGGc    >  2:1092288/1‑140 (MQ=255)
                                                                                                                                    tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATaacaaa                        >  2:1901575/1‑120 (MQ=255)
                                                                                                                                    tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATaacaaa                        <  1:1901575/120‑1 (MQ=255)
                                                                                                                                    tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              >  1:3189710/1‑130 (MQ=255)
                                                                                                                                    tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              <  2:2698566/130‑1 (MQ=255)
                                                                                                                                    tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc    >  2:2472756/1‑140 (MQ=255)
                                                                                                                                    tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc    >  1:1122996/1‑140 (MQ=255)
                                                                                                                                    tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc    >  1:385395/1‑140 (MQ=255)
                                                                                                                                    tCAGTATGTTGATAAGAACCGCAACATCAAACGACGGTTCATATGACGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTCTCCGGGAACCGTAATGACACACAGCTCCGCTCCCCTCAGGc    >  1:3118823/1‑140 (MQ=255)
                                                                                                                                      aGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCAc           <  1:1012813/131‑1 (MQ=255)
                                                                                                                                      aGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCAt  >  1:2833850/1‑140 (MQ=255)
                                                                                                                                      aGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCAt  >  1:263450/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                         
TTTCTGCCTCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCAT  >  NZ_CP034943/451988‑452261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: