Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP034943 1,176,173 G→T G135V (GGT→GTT)  med → transcriptional regulator Med

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,176,1730GT98.6% 795.3 / ‑0.9 215G135V (GGT→GTT) medtranscriptional regulator Med
Reads supporting (aligned to +/- strand):  ref base G (3/0);  new base T (129/83);  total (132/83)
Fisher's exact test for biased strand distribution p-value = 2.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 218 total aligned reads displayed.

CTATGGCCATGGGAGTGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAGAAGTCAAC  >  NZ_CP034943/1176036‑1176309
                                                                                                                                         |                                                                                                                                        
      ccAGGGGAGTGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTg                                                                                                                                  >  1:1813545/1‑140 (MQ=255)
         tGGGAGTGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGACGCAATGGTTTTTTTTggag                                                                                                                               >  2:3242700/1‑140 (MQ=255)
         tGGGAGTGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTggag                                                                                                                               >  1:2283636/1‑140 (MQ=255)
         tGGGAGTGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTggag                                                                                                                               >  2:602084/1‑140 (MQ=255)
           ggAGTGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTggagga                                                                                                                             >  1:158556/1‑140 (MQ=255)
               tGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGa                                                                                                                         >  2:1048870/1‑140 (MQ=255)
               tGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGa                                                                                                                         >  2:194006/1‑140 (MQ=255)
               tGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGa                                                                                                                         >  1:1685789/1‑140 (MQ=255)
                gAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGAc                                                                                                                        <  1:1866284/140‑1 (MQ=255)
                    atGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAAGGGTTTTTTTTGGAGGAATGACAGCt                                                                                                                    >  1:107222/1‑140 (MQ=255)
                     tGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTg                                                                                                                   <  1:2953639/140‑1 (MQ=255)
                      gCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGc                                                                                                                  >  2:1407831/1‑140 (MQ=255)
                         gagGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGACCa                                                                                                               >  2:1171213/1‑140 (MQ=255)
                          agGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCAt                                                                                                              >  1:1381376/1‑140 (MQ=255)
                           ggTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCACTGGTTTTTTTTGGAGGAATGACAGCTGCCCATc                                                                                                             >  1:2473951/1‑140 (MQ=255)
                           ggTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATc                                                                                                             >  2:2426571/1‑140 (MQ=255)
                               tttAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTc                                                                                                         >  1:1498080/1‑140 (MQ=255)
                               tttAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTc                                                                                                         >  1:9139/1‑140 (MQ=255)
                               tttAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTc                                                                                                         >  1:2826905/1‑140 (MQ=255)
                               tttAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTc                                                                                                         >  1:2199431/1‑140 (MQ=255)
                               tttAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTc                                                                                                         >  1:656338/1‑140 (MQ=255)
                                       ggTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAActa                                                                                                 >  1:561437/1‑138 (MQ=255)
                                       ggTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGa                                                                                                 >  2:743939/1‑140 (MQ=255)
                                       ggTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGa                                                                                                 >  1:3042174/1‑140 (MQ=255)
                                       ggTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGa                                                                                                 >  1:236650/1‑140 (MQ=255)
                                       ggTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGa                                                                                                 >  2:733374/1‑140 (MQ=255)
                                         tCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAAt                                                                                               >  2:70085/1‑140 (MQ=255)
                                            gCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAg                                                                                            <  1:1920148/140‑1 (MQ=255)
                                             cGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAgg                                                                                           >  1:232480/1‑140 (MQ=255)
                                             cGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAgg                                                                                           >  2:2164899/1‑140 (MQ=255)
                                              gAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGACGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGt                                                                                          <  1:3242700/140‑1 (MQ=255)
                                              gAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGt                                                                                          <  2:1268000/140‑1 (MQ=255)
                                              caaGATTATCGAGATATGGAATTCGTCAATTCTAATGCTAAAGCAAAAGCTGATACTGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGCAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGt                                                                                          <  1:3396373/139‑1 (MQ=255)
                                                     aTCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTg                                                                                   <  2:2700062/140‑1 (MQ=255)
                                                       ccAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGAt                                                                                 >  2:2156136/1‑140 (MQ=255)
                                                         aGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTg                                                                               <  1:1322888/140‑1 (MQ=255)
                                                         aGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTg                                                                               <  2:343031/140‑1 (MQ=255)
                                                          gATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGc                                                                              <  1:2916313/140‑1 (MQ=255)
                                                          gATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGc                                                                              <  2:2806540/140‑1 (MQ=255)
                                                           atatGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCt                                                                             >  1:2800541/1‑140 (MQ=255)
                                                           atatGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCt                                                                             >  1:1467883/1‑140 (MQ=255)
                                                            tatGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCtt                                                                            >  2:1702030/1‑140 (MQ=255)
                                                            tatGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCtt                                                                            >  2:1455075/1‑140 (MQ=255)
                                                             atGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGc                                                                              <  1:1255410/137‑1 (MQ=255)
                                                             atGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGc                                                                              >  2:1255410/1‑137 (MQ=255)
                                                                     cGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGt                                                                   >  2:1622391/1‑140 (MQ=255)
                                                                     cGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGt                                                                   >  1:2724283/1‑140 (MQ=255)
                                                                     cGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGt                                                                   >  2:3052046/1‑140 (MQ=255)
                                                                      gTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTg                                                                  <  2:158556/140‑1 (MQ=255)
                                                                       tCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTgg                                                                 <  1:67389/140‑1 (MQ=255)
                                                                           ttCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGc                                                             <  2:2333660/140‑1 (MQ=255)
                                                                           ttCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGc                                                             <  1:1037134/140‑1 (MQ=255)
                                                                           ttCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGc                                                             <  1:1972893/140‑1 (MQ=255)
                                                                                aTGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAg                                                        >  1:851828/1‑140 (MQ=255)
                                                                                aTGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAg                                                        >  1:639786/1‑140 (MQ=255)
                                                                                     aaaGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGa                                                   >  1:2886794/1‑140 (MQ=255)
                                                                                      aaGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTggagg                                                                                                                              >  2:1195783/1‑64 (MQ=255)
                                                                                      aaGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTggagg                                                                                                                              <  1:1195783/64‑1 (MQ=255)
                                                                                      aaGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGAc                                                  >  2:2862695/1‑140 (MQ=255)
                                                                                       aGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACg                                                 >  2:2993996/1‑140 (MQ=255)
                                                                                         cAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCACTGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTGTACGTGGCAGCCGGAGGTAGACGGt                                               <  1:16059/140‑1 (MQ=255)
                                                                                         cAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGt                                               <  2:2628639/140‑1 (MQ=255)
                                                                                         cAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGt                                               <  1:2049634/140‑1 (MQ=255)
                                                                                            aaGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAg                                                    >  2:528338/1‑132 (MQ=255)
                                                                                            aaGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGa                                                   <  2:3107843/133‑1 (MQ=255)
                                                                                            aaGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGtttt                                            >  1:993990/1‑140 (MQ=255)
                                                                                            aaGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATGGCTTCCTTTACGTGGCAGCCGGAGGTCGACGGTTTTa                                           >  2:2723039/1‑140 (MQ=255)
                                                                                             aGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTAGGTGGCAGCCGGAGGTAGACGGTTTTa                                           >  1:2753975/1‑140 (MQ=255)
                                                                                                        tgACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGcc                                                                                                                 >  1:3029988/1‑59 (MQ=255)
                                                                                                        tgACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGcc                                <  2:1498080/140‑1 (MQ=255)
                                                                                                         gACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTATACGGTTTTATCAAAGGGGCCa                               >  1:261790/1‑140 (MQ=255)
                                                                                                         gACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCa                               >  2:2076341/1‑140 (MQ=255)
                                                                                                         gACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCa                               >  2:3076659/1‑140 (MQ=255)
                                                                                                         gACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCa                               >  1:657958/1‑140 (MQ=255)
                                                                                                         gACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCa                               >  1:327983/1‑140 (MQ=255)
                                                                                                         gACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCa                               >  1:3159332/1‑140 (MQ=255)
                                                                                                          aCGAGTGTCCATTTCAGCGGCGCAGCAATGGTTTTTTTTGGAGGAAGGACAGCTGCCCCTCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGTCGGTTTTATCAAAGGGGGCaa                              >  2:3027766/1‑140 (MQ=255)
                                                                                                          aCGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCac                              >  2:855769/1‑139 (MQ=255)
                                                                                                          aCGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCaa                              >  1:2145336/1‑140 (MQ=255)
                                                                                                          aCGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCaa                              >  1:382500/1‑140 (MQ=255)
                                                                                                          aCGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCaa                              <  1:2519374/140‑1 (MQ=255)
                                                                                                               tgtCCATTTCAGCGGCGAAGCAATGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGa                        >  2:2234976/1‑140 (MQ=255)
                                                                                                                 tCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATg                         >  2:1362100/1‑138 (MQ=255)
                                                                                                                 tCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGaa                       >  1:2879504/1‑140 (MQ=255)
                                                                                                                 tCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGaa                       >  1:3372624/1‑140 (MQ=255)
                                                                                                                 tCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGaa                       >  2:2191086/1‑140 (MQ=255)
                                                                                                                 tCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCCAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTGTTATCACAGGGGCCAAATATGaa                       >  1:2999870/1‑140 (MQ=255)
                                                                                                                  ccATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCATGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGaaa                      <  1:891249/140‑1 (MQ=255)
                                                                                                                    aTTTCAGCGGCGAAGCAATTGTTTTTTTTGGAGGATTGACAGCTGCCCATCTGTCGAAAACGAATAAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGTTAGACGGTTTTATCAAAGGGGCCAAATATGTAAAt                    <  2:2027014/140‑1 (MQ=255)
                                                                                                                    aTTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAAt                    <  2:293984/140‑1 (MQ=255)
                                                                                                                    aTTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAAt                    <  2:1613752/140‑1 (MQ=255)
                                                                                                                    aTTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAAt                    <  2:1381376/140‑1 (MQ=255)
                                                                                                                           cggcgAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGata             >  1:705320/1‑140 (MQ=255)
                                                                                                                           cggcgAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTGTACGTGGCAGCGGGAGGTAGACGGGTTTATCACAGGGGCCCAATATGAAAATCCGGata             >  2:2557902/1‑140 (MQ=255)
                                                                                                                              cgAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAg          >  2:332405/1‑140 (MQ=255)
                                                                                                                                  gCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAGAAGt      <  1:3076659/140‑1 (MQ=255)
                                                                                                                                  gCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAGAAGt      <  1:1622391/140‑1 (MQ=255)
                                                                                                                                      tGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAGAAGTCAac  <  1:1779539/140‑1 (MQ=255)
                                                                                                                                      tGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAGAAGTCAac  <  2:639786/140‑1 (MQ=255)
                                                                                                                                      tGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAGAAGTCAac  <  2:851828/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
CTATGGCCATGGGAGTGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAGAAGTCAAC  >  NZ_CP034943/1176036‑1176309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: