Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP034943 3,247,065 C→T intergenic (‑11/+110) bdbD ← / ← cadA disulfide bond formation protein BdbD/cadmium‑translocating P‑type ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,247,0650CT98.8% 900.3 / NA 249intergenic (‑11/+110)bdbD/cadAdisulfide bond formation protein BdbD/cadmium‑translocating P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  major base T (160/84);  minor base G (3/0);  total (164/85)
Fisher's exact test for biased strand distribution p-value = 5.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 249 total aligned reads displayed.

GTCCGGAAACCGCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTCATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGCATGACG  >  NZ_CP034943/3246928‑3247199
                                                                                                                                         |                                                                                                                                      
 tCCGGAAACCGCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATc                                                                                                                                     >  1:1403409/1‑140 (MQ=255)
            cTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTttaaaatt                                                                                                                          >  2:971308/1‑140 (MQ=255)
            cTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTttaaaatt                                                                                                                          >  2:1532573/1‑140 (MQ=255)
            cTTCATTGCCTTGTTCCGTTTGGTTATTAAAGATGACAATGGCTGCTAGGAAGACAACAACCACAACCGTAAGGATGACCGCGAAGTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTAGCGTTttaaaatt                                                                                                                          >  2:131506/1‑140 (MQ=255)
                 ttGCTTTTTTCGGTTTTTTTTTTAAATATCACATTGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCTAATTTCGCAGAAGACTGCTTTTTCTTTTACACTCCGACACCTTATCTTTTTAAAATTaaaaa                                                                                                                     <  1:474154/140‑1 (MQ=255)
                 ttGCGTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGATTTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATGGTTTTAAAATTaaaaa                                                                                                                     <  1:1747599/140‑1 (MQ=255)
                       tGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAaagaag                                                                                                               <  1:1112684/140‑1 (MQ=255)
                       tGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAaagaag                                                                                                               >  2:9672/1‑140 (MQ=255)
                       tGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAaagaag                                                                                                               <  2:1626428/140‑1 (MQ=255)
                       tGTTCCGTTTGGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAaagaag                                                                                                               >  1:1596299/1‑140 (MQ=255)
                         ttCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCg                                                                                                             >  2:1427043/1‑140 (MQ=255)
                           cctttttGTTTTTAAAGATGAAAATGGATGATATTAATAAAAAAACCAAAACCTTATGTATGACCGCTAATTTCGCATAAGACTGCTGTTTATTTTTCACTCCGACACATTATCGTTTTAAAATTAAAAAAAGAAGCgta                                                                                                           <  2:1148143/137‑3 (MQ=255)
                           ccGTTTTGTTATTAACGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGa                                                                                                           <  1:1105432/140‑1 (MQ=255)
                            ctttttttTATTAAAGATGAAAATGTCTGCTAGTAAGAAAACAACAACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCTTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                          <  1:1358526/138‑1 (MQ=255)
                            cGTTTTGTTATTAAAGATGCCAATGGCTGCTAGTAAGACAACAACCACAACCGTACGGATGACCGCGAATTTCGGAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                          <  1:2489238/140‑1 (MQ=255)
                            cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                          <  1:845979/140‑1 (MQ=255)
                            cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                          <  2:1492442/140‑1 (MQ=255)
                            cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                          <  1:2871151/140‑1 (MQ=255)
                            cGTTGTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                          <  2:1106531/140‑1 (MQ=255)
                              ttttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAATAAGCGGATCg                                                                                                        >  1:80955/1‑140 (MQ=255)
                              ttttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCg                                                                                                        >  2:607958/1‑140 (MQ=255)
                              ttttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCg                                                                                                        >  2:2083045/1‑140 (MQ=255)
                              ttttGTCATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCg                                                                                                        >  1:2965336/1‑140 (MQ=255)
                               tttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGc                                                                                                       <  2:1181152/140‑1 (MQ=255)
                                ttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCa                                                                                                      >  1:1315431/1‑140 (MQ=255)
                                        aaaGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                          >  1:517528/1‑128 (MQ=255)
                                        aaaGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGAccc                                                                                              >  2:1724527/1‑140 (MQ=255)
                                         aaGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACATTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCa                                                                                             <  1:2337888/140‑1 (MQ=255)
                                             tGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAATCGGATCGCAATAGACCCActtg                                                                                         >  2:1993129/1‑139 (MQ=255)
                                             tGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCActtc                                                                                         >  1:3089452/1‑140 (MQ=255)
                                              gACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCActtct                                                                                        >  1:1538534/1‑140 (MQ=255)
                                              gACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCActtct                                                                                        >  1:637276/1‑140 (MQ=255)
                                                     gctgctAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTAtttt                                                                                 <  1:2727917/140‑1 (MQ=255)
                                                        gctAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAac                                                                              <  1:956842/140‑1 (MQ=255)
                                                        gctAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAac                                                                              <  2:874572/140‑1 (MQ=255)
                                                        gctAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAac                                                                              <  2:2651988/140‑1 (MQ=255)
                                                        gctAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAac                                                                              <  1:52463/140‑1 (MQ=255)
                                                           aGTAAGACACCAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGTCTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAATCGGATCGCAATAGACACACTTCTTTATTTTAacaag                                                                           >  1:1521074/1‑140 (MQ=255)
                                                               aGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaagacaa                                                                       >  2:183587/1‑140 (MQ=255)
                                                               aGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaagacaa                                                                       >  1:2205511/1‑140 (MQ=255)
                                                               aGACAACAACCACAACCGTAAGGAGGACCGCGAATTTCGCAGAAGACTGCTGTTTATTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaagacaa                                                                       <  1:3009301/140‑1 (MQ=255)
                                                                 acaacaACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGt                                                                     <  1:407192/140‑1 (MQ=255)
                                                                    acaaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGcc                                                                  <  2:1565358/140‑1 (MQ=255)
                                                                    acaaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGcc                                                                  <  2:2622350/140‑1 (MQ=255)
                                                                    acaaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCGTATCGTGTTAAAATTAAAAAAAGAAGCGGATCGCACTAGACCCCCTGCTTTATTTTATCAAGACAAGTGcc                                                                  >  2:1666713/1‑140 (MQ=255)
                                                                     caaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTGTCACTCCGACACCTTATCGTTTTAAAATTAAAAATACAAGCGGAGCGCAATAGACCCACGTCTTTATTGTAACCATACAAGTGCCt                                                                 >  2:1221489/1‑140 (MQ=255)
                                                                            aaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGaa                                                          >  1:3025577/1‑140 (MQ=255)
                                                                                gTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAAcc                                                      >  1:1723511/1‑140 (MQ=255)
                                                                                gTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAAcc                                                      >  1:74104/1‑140 (MQ=255)
                                                                                 tAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAAccc                                                     >  2:2575257/1‑140 (MQ=255)
                                                                                 tAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAAccc                                                     >  1:2798397/1‑140 (MQ=255)
                                                                                      aTGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTaa                                                                               >  2:2371605/1‑109 (MQ=255)
                                                                                      aTGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTaa                                                                               <  1:2371605/109‑1 (MQ=255)
                                                                                      aTGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaa                                                >  1:2911114/1‑140 (MQ=255)
                                                                                        gACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaa                                              >  2:1767861/1‑140 (MQ=255)
                                                                                        gACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaa                                              >  2:2419013/1‑140 (MQ=255)
                                                                                        gACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaa                                              >  2:2318771/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                          >  1:2540713/1‑79 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAaca                                                                             >  1:2888848/1‑108 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAaca                                                                             <  2:2888848/108‑1 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAATTGCCTATAAGAAAACCCCGaacaaa                                             >  1:2442946/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  2:3112820/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  2:3202189/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  2:3048586/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  2:2529099/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  2:2183001/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  2:301828/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  2:478539/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  1:1016823/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  1:2574974/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  1:2624917/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  2:1200656/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  1:2685172/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  2:1595630/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  1:825057/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  1:2114475/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTAAAAGACAACCCCGaaaaaa                                             >  1:1911320/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTGCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  1:1157098/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTAGCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAACTGCCTATAAGACAACCCCGaaaaaa                                             >  2:452463/1‑140 (MQ=255)
                                                                                         aCCGCGAATTTCGCAGAAGACTACTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  2:1372178/1‑140 (MQ=255)
                                                                                          ccGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAAt                                            >  1:377608/1‑140 (MQ=255)
                                                                                          ccGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAAt                                            >  1:377603/1‑140 (MQ=255)
                                                                                           cgcgAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaa                                                 <  1:3072973/134‑1 (MQ=255)
                                                                                             cgAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGaa                                         <  1:989682/140‑1 (MQ=255)
                                                                                             agAATTTAGCAGAAGACTGCTGTTTCTTTTTCACTCCGACAACTTTTATTTTTAAAATTAAAAAAAGAAGCTGATAGCAATAGACACAATTCTTTATTTTAACAAGAGTTGTTTATATAAGAAAACCCCGAAAAATTGaa                                         <  2:1911320/139‑1 (MQ=255)
                                                                                                      cAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAACAAAACCCCGAAAAAATGAATTCTCTCCg                                <  1:3270496/140‑1 (MQ=255)
                                                                                                              tgctgTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                          >  1:1578048/1‑58 (MQ=255)
                                                                                                              tgctgTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTa                        >  2:2470130/1‑140 (MQ=255)
                                                                                                                ctgTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGAcc                                                                                               >  2:2974329/1‑67 (MQ=255)
                                                                                                                   tttCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGt                   >  1:1669362/1‑140 (MQ=255)
                                                                                                                     tCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGttt                 >  2:2830760/1‑140 (MQ=255)
                                                                                                                        ttttCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTg              >  2:3269676/1‑140 (MQ=255)
                                                                                                                         tttCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTga             <  1:3323826/140‑1 (MQ=255)
                                                                                                                         tttCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTga             <  2:1063991/140‑1 (MQ=255)
                                                                                                                          ttCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTgag            >  1:653435/1‑140 (MQ=255)
                                                                                                                           tCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTgaga           <  1:1768194/140‑1 (MQ=255)
                                                                                                                              cTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGc        <  1:2183001/140‑1 (MQ=255)
                                                                                                                                   acacATTATCGTTATAAAAATAAAAAAAGAAGCGGATCGCAATAGACCGAGTTCTTTATATTAACGAGACAAGTGCCTATAAGACAACCCCGAAACAATGAATGCTCGCCGACAAGTTATCCGTTTTTCAGACGCATGAc   <  2:1856984/140‑1 (MQ=255)
                                                                                                                                    cacCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGCATGAcg  <  2:377608/140‑1 (MQ=255)
                                                                                                                                    aacCTTATCGTTTTAAAATTAAAAAAAGAAGCGCCTCGCAATACACCCACTTCTTTATTTTAACAAGACACGTGCATATAAGAAAACCCCGAAAAAATGAATACTCTCCGACAATTTATCCGTTTTTGAGACGCATGAcg  <  2:377543/139‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
GTCCGGAAACCGCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTCATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGCATGACG  >  NZ_CP034943/3246928‑3247199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: