Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP034943 3,637,344 T→A Y44N (TAC→AAC)  EO946_RS18740 → DUF2529 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,637,3440TA98.4% 905.7 / ‑5.4 250Y44N (TAC→AAC) EO946_RS18740DUF2529 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (127/119);  minor base C (0/4);  total (127/123)
Fisher's exact test for biased strand distribution p-value = 5.71e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 259 total aligned reads displayed.

TTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGTTTTGCTCAGGGAC  >  NZ_CP034943/3637208‑3637480
                                                                                                                                        |                                                                                                                                        
ttCACTTATGTTGAAAATTTTTACGACGCAGTTAACAGGTATTTTGTCCCACATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTGCATTTATTTAAACg                                                                                                                                       <  1:3125191/140‑1 (MQ=255)
ttCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACg                                                                                                                                       <  2:2883180/140‑1 (MQ=255)
    cTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGc                                                                                                                                   <  2:1551014/140‑1 (MQ=255)
    cTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGc                                                                                                                                   <  2:1171676/140‑1 (MQ=255)
      tATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTa                                                                                                                                 <  2:1850500/140‑1 (MQ=255)
      tATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTa                                                                                                                                 <  2:1526875/140‑1 (MQ=255)
        tcttGAAAATAATTACGACGCAGTGAACAGGTAGTTTGTCCCGCATTCAGGATAAGGAATCTGAAGCGATTGAAGATGGGGCGCTGCTGATTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAAt                                                                                                                               <  1:1215562/138‑1 (MQ=255)
        tGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTCAt                                                                                                                               >  2:3214467/1‑140 (MQ=255)
        tGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAAt                                                                                                                               <  2:2516404/140‑1 (MQ=255)
        tGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAAt                                                                                                                               <  2:749619/140‑1 (MQ=255)
        tGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAAt                                                                                                                               <  2:2765662/140‑1 (MQ=255)
        tGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAAt                                                                                                                               <  1:2563777/140‑1 (MQ=255)
        tGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCAGTCAGTATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAAt                                                                                                                               <  1:171970/140‑1 (MQ=255)
             aaatTATTTCCGACGCAGTGCACCGGTATTTGTTCCCGCAGTCCGGATAAGGTATCTGACGGGAGTGGCGATGGGGCGCGGCTGCTTGCGCCCGCGGGGCTCCGCGGGCTTTCCCTGTCGGTAAACGGAGCTAGTGTGcg                                                                                                                          >  2:1890514/1‑139 (MQ=255)
             aaaaTATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCt                                                                                                                          >  1:1513621/1‑140 (MQ=255)
                   tttACGACGCAGTTACCCGGTATTTTTTCCCGCCTTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAggg                                                                                                                    <  1:3164391/140‑1 (MQ=255)
                   tttACGACGCAGTTAACATGTATTTTTTCCCGAATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAggg                                                                                                                    <  2:2075562/140‑1 (MQ=255)
                   tttACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAggg                                                                                                                    <  1:2666880/140‑1 (MQ=255)
                   tttACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAggg                                                                                                                    <  1:101325/140‑1 (MQ=255)
                   tttACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAggg                                                                                                                    <  2:1070784/140‑1 (MQ=255)
                   tttACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAggg                                                                                                                    <  1:417882/140‑1 (MQ=255)
                   tttACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAggg                                                                                                                    <  2:3328356/140‑1 (MQ=255)
                   tttACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAggg                                                                                                                    <  2:658524/140‑1 (MQ=255)
                      acgacgCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGt                                                                                                                 <  2:2972260/140‑1 (MQ=255)
                          cgCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCttt                                                                                                             >  1:878452/1‑140 (MQ=255)
                            cAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTa                                                                                                           >  1:41900/1‑140 (MQ=255)
                                 aaCAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAACCGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAgg                                                                                                      <  2:1284084/140‑1 (MQ=255)
                                 aaCAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAgg                                                                                                      <  1:3245635/140‑1 (MQ=255)
                                 aaCAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAgg                                                                                                      <  1:2934243/140‑1 (MQ=255)
                                 aaCAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAgg                                                                                                      <  2:2736035/140‑1 (MQ=255)
                                 aaCAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAgg                                                                                                      <  1:1944225/140‑1 (MQ=255)
                                 aaCAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAgg                                                                                                      <  1:1873000/140‑1 (MQ=255)
                                 aaCAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAgg                                                                                                      <  2:55592/140‑1 (MQ=255)
                                      gTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCAcc                                                                                                 >  1:857726/1‑140 (MQ=255)
                                       tATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCg                                                                                                >  1:3285386/1‑140 (MQ=255)
                                       tATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCg                                                                                                >  2:2347315/1‑140 (MQ=255)
                                         ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACgg                                                                                                                                      >  1:2670513/1‑100 (MQ=255)
                                         ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaa                                                                                              >  1:1994120/1‑140 (MQ=255)
                                         ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaa                                                                                              >  2:821974/1‑140 (MQ=255)
                                         ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCGAATGAGCTTCAGGGCGTCTGTTATGAGGCCACCGaa                                                                                              >  1:2398174/1‑140 (MQ=255)
                                          tttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaaa                                                                                             <  2:222135/140‑1 (MQ=255)
                                            tttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAagc                                                                                           >  1:1061540/1‑140 (MQ=255)
                                                ccGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCg                                                                                       >  2:1002552/1‑140 (MQ=255)
                                                ccGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCg                                                                                       <  1:2841645/140‑1 (MQ=255)
                                                 cGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGa                                                                                      >  1:2876985/1‑140 (MQ=255)
                                                 cGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTGAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGa                                                                                      >  1:514161/1‑140 (MQ=255)
                                                   cATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAAc                                                                                    >  2:3378031/1‑140 (MQ=255)
                                                    aTTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAAcc                                                                                   <  1:454184/140‑1 (MQ=255)
                                                      tCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCt                                                                                 <  2:2033433/140‑1 (MQ=255)
                                                            tAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCAt                                                                           <  2:1768860/140‑1 (MQ=255)
                                                            tAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCAt                                                                           <  1:3356120/140‑1 (MQ=255)
                                                            tAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCAt                                                                           <  1:2299800/140‑1 (MQ=255)
                                                              aGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCt                                                                         >  1:630630/1‑140 (MQ=255)
                                                               ggAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAAt                                                                                                                               >  1:625693/1‑85 (MQ=255)
                                                               ggAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAAt                                                                                                                               <  2:625693/85‑1 (MQ=255)
                                                                   tCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaa                                                                    >  1:1986661/1‑140 (MQ=255)
                                                                    cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                   >  2:3001632/1‑140 (MQ=255)
                                                                    cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                   >  1:1600964/1‑140 (MQ=255)
                                                                    cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                   >  2:2630682/1‑140 (MQ=255)
                                                                    cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                   >  1:1785105/1‑140 (MQ=255)
                                                                    cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                   >  1:1859150/1‑140 (MQ=255)
                                                                    cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                   >  2:830459/1‑140 (MQ=255)
                                                                     tGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAg                                                                  <  2:1260019/140‑1 (MQ=255)
                                                                           gATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAACGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTc                                                            >  1:2479464/1‑140 (MQ=255)
                                                                             ttGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCa                                                          >  1:3213762/1‑140 (MQ=255)
                                                                                  gctGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTGAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTCTGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAg                                                     <  1:979093/138‑1 (MQ=255)
                                                                                     ggggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGaa                                                                                     <  1:1963628/105‑1 (MQ=255)
                                                                                     ggggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAgcgc                                                  >  2:3268833/1‑140 (MQ=255)
                                                                                     ggggCGCGGCTGCTTGCTCAACCGGTGATCAGCGGGCATTCCATTTATATAAACGGAGCTAATGAGCTTCAGTGCGTCTTTTATGAGGCCACCGAAACCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAgcgc                                                  >  1:3313385/1‑140 (MQ=255)
                                                                                       ggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAgg                                                                                                      <  1:1344585/86‑1 (MQ=255)
                                                                                       ggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGACAGCGCTg                                                >  1:1838350/1‑140 (MQ=255)
                                                                                       ggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCtc                                                >  2:1978086/1‑139 (MQ=255)
                                                                                       ggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTg                                                >  2:2267127/1‑140 (MQ=255)
                                                                                          gcgGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAgg                                             <  1:2544521/140‑1 (MQ=255)
                                                                                                        aaGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGc                               >  2:437189/1‑140 (MQ=255)
                                                                                                            tgTGATCAGCGGGCATTCAATTTATTTAAACGGAGCTAAGGAGGTTCAGGGCGTCTTTTTTGAGCCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAACCGCTGTGGAAGTGACAGAAAGCGACa                           <  2:54439/139‑1 (MQ=255)
                                                                                                            ggTTATCCGCGGGAATTCAATTTATTTAAACGGAGCTAATGAGCTTCTGGGCGTATTTTATGAGGCCACCCAAAGCAGCGAACCCTTCCCATCTGTAAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACACAACGCGACa                           <  1:655178/140‑1 (MQ=255)
                                                                                                            ggTGCTCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACa                           <  2:2001147/140‑1 (MQ=255)
                                                                                                            ggTGATCAGCGGGCATTCCTTTTATTTAACCGGAGCTAATCAGGTTCAGGGCGTCTTTTATGAGGCCACCTAAAGCAGCGAACCCTTCCCATCTGTCAACGCCTTTCCCGAAAGCGCTGAGGATGTGACAGAAAGCGACa                           <  2:3074728/140‑1 (MQ=255)
                                                                                                            ggTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACa                           <  2:2668531/140‑1 (MQ=255)
                                                                                                            ggTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCACAAAGCGCTGAGGAATTGACAGAAAGCGACa                           <  2:2263208/140‑1 (MQ=255)
                                                                                                            ggTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTATTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACa                           <  2:604806/140‑1 (MQ=255)
                                                                                                             gtcaTCAGCGGGCTTTCCATTTATTTAAACGGAGCTAATTCGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAg                          <  2:1341606/137‑1 (MQ=255)
                                                                                                              tGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCttt                                                             <  1:3041219/104‑1 (MQ=255)
                                                                                                                aTCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGACAGCGCTGAGGAAGTGACAGAAAGCGACAGGGt                       >  2:1279178/1‑140 (MQ=255)
                                                                                                                aTCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGt                       >  2:464157/1‑140 (MQ=255)
                                                                                                                aTCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGt                       >  2:927238/1‑140 (MQ=255)
                                                                                                                aTCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGt                       >  1:2223187/1‑140 (MQ=255)
                                                                                                                aTCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGt                       >  2:163974/1‑140 (MQ=255)
                                                                                                                    gCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAACGCCTTTCCCGAAAGCGCGGAGGAAGGGACAGCACGCGACAggggggtg                   >  2:1948627/1‑135 (MQ=255)
                                                                                                                        gCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGt               >  2:376887/1‑140 (MQ=255)
                                                                                                                        gCATGCCATTTATTTAAACGGAGCTACTGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAACGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGt               <  2:2424802/140‑1 (MQ=255)
                                                                                                                         cATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAACGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGtt              <  2:713987/140‑1 (MQ=255)
                                                                                                                          aTTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGttt             >  1:3167564/1‑140 (MQ=255)
                                                                                                                          aTTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGGGCTGagggtt             >  1:2079498/1‑135 (MQ=255)
                                                                                                                             ccATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGTTTTGc          >  2:511778/1‑140 (MQ=255)
                                                                                                                                tttatttaCACGGAGCTAATGCGCTTCAGGGCTTCTTTTATGCGCCCACCCAAAGCAGCGAACCGTTCCAATCTGTCAACGCCTTCCCACAACGCGCTGAGCACGTGACAGAACGCGACAGGGTGCTGATGTTTTGCTCa       <  1:1127642/140‑1 (MQ=255)
                                                                                                                                tttatttaAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGTTTTGCTCa       <  2:2926494/140‑1 (MQ=255)
                                                                                                                                tttatttaAACGGAGCTAATGAGATTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCCAACCGTGCCAATCTGTCAAAGCCTTTCCACAAAGCGCTGAGTATGTGAAAGAAAGCGACAGGGTGCTGATGTTTTGCTCa       <  1:3072411/140‑1 (MQ=255)
                                                                                                                                     ttaaACGGAGCTAATGAGGTTCTGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCAACTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTCACAGAAAGCGACAGGGTGCTGATGTTTTGCTCAGGGAc  <  1:27098/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
TTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGTTTTGCTCAGGGAC  >  NZ_CP034943/3637208‑3637480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: