Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP034943 3,637,344 T→A Y44N (TAC→AAC)  EO946_RS18740 → DUF2529 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,637,3440TA99.0% 767.2 / NA 210Y44N (TAC→AAC) EO946_RS18740DUF2529 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (105/102);  minor base C (1/1);  total (107/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

TTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGTTTTGCTCAGGG  >  NZ_CP034943/3637208‑3637478
                                                                                                                                        |                                                                                                                                      
ttCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACg                                                                                                                                     >  1:1031818/1‑140 (MQ=255)
      tATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTa                                                                                                                               <  2:434343/140‑1 (MQ=255)
        tGTTGAAAATATTTACGACGCCGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCAGTTATTTAAACGGAGCTAAt                                                                                                                             >  2:1087606/1‑140 (MQ=255)
        tGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAAt                                                                                                                             <  2:1031818/140‑1 (MQ=255)
        tGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAAt                                                                                                                             <  1:2461000/140‑1 (MQ=255)
        tGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAAt                                                                                                                             >  1:2203026/1‑140 (MQ=255)
        tGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAAt                                                                                                                             <  2:2203026/140‑1 (MQ=255)
        tGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGAAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAAt                                                                                                                             <  2:2008183/140‑1 (MQ=255)
          ttGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGa                                                                                                                           >  1:2790834/1‑140 (MQ=255)
           tGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAg                                                                                                                          <  1:2388170/140‑1 (MQ=255)
             agaaTATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCt                                                                                                                        >  1:1804900/3‑140 (MQ=255)
             aaaaTATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCt                                                                                                                        >  1:1107658/1‑140 (MQ=255)
             aaaaTATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGAGGGCATTACATTTAGTTAAACGGAGCTAATGAGCt                                                                                                                        >  1:2588379/1‑140 (MQ=255)
                  atTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACgg                                                                                                                                    >  1:1327919/1‑123 (MQ=255)
                   tttACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAggg                                                                                                                  <  1:1474862/140‑1 (MQ=255)
                   tttACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAggg                                                                                                                  <  2:566783/140‑1 (MQ=255)
                     tACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCg                                                                                                                <  2:768326/140‑1 (MQ=255)
                                 aaCAGGTATTTTTTCCCGCATTCAGGTTAAGGAATCTGACGCGATTGATGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTCAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAgg                                                                                                    <  1:91612/140‑1 (MQ=255)
                                    aGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGt                                                                                                               <  1:2074935/126‑1 (MQ=255)
                                    aGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAgg                                                                                                    >  1:3066277/1‑137 (MQ=255)
                                    aGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAgg                                                                                                    <  2:3066277/137‑1 (MQ=255)
                                       tATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCg                                                                                              >  1:751006/1‑140 (MQ=255)
                                         ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaa                                                                                            >  2:2910763/1‑140 (MQ=255)
                                         ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaa                                                                                            >  2:260847/1‑140 (MQ=255)
                                         ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaa                                                                                            >  2:2880930/1‑140 (MQ=255)
                                         ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaa                                                                                            >  1:1369500/1‑140 (MQ=255)
                                         ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaa                                                                                            >  2:828610/1‑140 (MQ=255)
                                         ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaa                                                                                            >  1:1607716/1‑140 (MQ=255)
                                         ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGa                                                                                             >  2:1794795/1‑139 (MQ=255)
                                         ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGACCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaa                                                                                            >  2:3409001/1‑140 (MQ=255)
                                              tCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAagcag                                                                                       >  1:2924399/1‑140 (MQ=255)
                                                ccGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCg                                                                                     <  1:2623548/140‑1 (MQ=255)
                                                ccGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCg                                                                                     <  2:1804900/140‑1 (MQ=255)
                                                ccGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCg                                                                                     <  1:2846646/140‑1 (MQ=255)
                                                ccGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCg                                                                                     <  2:1050746/140‑1 (MQ=255)
                                                  gCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGaa                                                                                   <  2:1508574/140‑1 (MQ=255)
                                                  gCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGaa                                                                                   <  2:581537/140‑1 (MQ=255)
                                                  gCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGaa                                                                                   <  2:1583076/140‑1 (MQ=255)
                                                          gATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTGTTATGAGGCCACCGAAAGCAGCGAACCCTTccc                                                                           >  1:1990040/1‑140 (MQ=255)
                                                              aGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCt                                                                       >  1:3331638/1‑140 (MQ=255)
                                                              aGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCt                                                                       >  1:489543/1‑140 (MQ=255)
                                                               ggAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACgg                                                                                                                                    <  2:3292693/78‑1 (MQ=255)
                                                               ggAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATGTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTg                                                                      >  2:240939/1‑140 (MQ=255)
                                                                   tCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaa                                                                  <  1:1898232/140‑1 (MQ=255)
                                                                   tCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaa                                                                  <  2:2924422/140‑1 (MQ=255)
                                                                   tCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaa                                                                  <  1:150857/140‑1 (MQ=255)
                                                                    cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAGGCAGCGAACCCTTCCCATCTGTCaaa                                                                 >  2:1956430/1‑140 (MQ=255)
                                                                    cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaac                                                                 >  2:2542883/1‑139 (MQ=255)
                                                                    cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                 >  2:3151707/1‑140 (MQ=255)
                                                                    cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                 >  1:3103635/1‑140 (MQ=255)
                                                                    cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                 >  1:1026488/1‑140 (MQ=255)
                                                                     tGACGCGATTGCAGATGGTGCGAGGCTGCTTGCTCAAGCGTTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGTGCGTATTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAg                                                                <  2:208155/140‑1 (MQ=255)
                                                                        cgagATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTAAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCt                                                             >  1:584024/4‑140 (MQ=255)
                                                                          cgATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCttt                                                           <  2:1400163/140‑1 (MQ=255)
                                                                             ttGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCa                                                        >  1:2033705/1‑140 (MQ=255)
                                                                                  gATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAg                                                   <  1:387766/140‑1 (MQ=255)
                                                                                       ggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAAt                                                                                                                             >  2:656335/1‑61 (MQ=255)
                                                                                       ggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAAt                                                                                                                             <  1:656335/61‑1 (MQ=255)
                                                                                       ggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCttt                                                           <  2:2126414/127‑1 (MQ=255)
                                                                                       ggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCttt                                                           >  1:2126414/1‑127 (MQ=255)
                                                                                       ggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTg                                              >  1:1419666/1‑140 (MQ=255)
                                                                                       ggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTg                                              >  1:1207122/1‑140 (MQ=255)
                                                                                       ggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTg                                              >  1:888628/1‑140 (MQ=255)
                                                                                       ggCGCCGCTGCTTTCTAAACCCGGGAACAGCCGGCAATACATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCATTTATGAGGCCAACGAAAGCACCGAACCCTTCCCAAAAGAAAAAGCCTTTACAaaaacaccag                                              >  2:2319303/1‑134 (MQ=255)
                                                                                           cgGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGa                                          >  1:2963731/1‑140 (MQ=255)
                                                                                           cgGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGa                                          >  1:2559110/1‑140 (MQ=255)
                                                                                             gctgctTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAg                                        >  1:1085303/1‑140 (MQ=255)
                                                                                             gctgctTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAg                                        >  1:33774/1‑140 (MQ=255)
                                                                                                gcttgctCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGa                                     <  2:1290271/140‑1 (MQ=255)
                                                                                                gcttgctCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGa                                     <  2:2255504/140‑1 (MQ=255)
                                                                                                 cttgctCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGa                                                      <  2:3165132/122‑1 (MQ=255)
                                                                                                 cttgctCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGa                                                      >  1:3165132/1‑122 (MQ=255)
                                                                                                       aacggggTTATCTGCGGGCATTACATTTATTTAAAGGTAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGGTGAGGAAGTGACACAAAg                              <  2:1440981/135‑1 (MQ=255)
                                                                                                            gttGATCAGCGGCCATTCCATTTATTTAACCGGAGCTAATGTGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACa                         <  2:2048787/138‑1 (MQ=255)
                                                                                                            ggTTATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCAATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACa                         <  2:2161911/140‑1 (MQ=255)
                                                                                                            ggTTATCAGCGGGCATTCCATTTATTTAAACGGAGATAATGAGCTTCAGGGCGTATTTTATGAGGCCACCTAACGCAGCGAACCCTTCCCATCTGTCAACCCCTTTCCACAACGCGCTGAGGACGTGACAGAAAGCGACa                         <  1:1763915/140‑1 (MQ=255)
                                                                                                            ggTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCCGAAAGCGCTGAGGAAGTGACAGAAAGCGACa                         <  2:1607716/140‑1 (MQ=255)
                                                                                                            ggTGATCAGCGGGCATTCAATTTATTTAAACGGAGCTACTGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACa                         <  2:1711221/140‑1 (MQ=255)
                                                                                                            ggTGATCAGCGGGCATTACATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACa                         <  1:3304515/140‑1 (MQ=255)
                                                                                                             gTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTACTGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAg                        >  2:3256720/1‑140 (MQ=255)
                                                                                                             gTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAg                        >  2:90606/1‑140 (MQ=255)
                                                                                                             gTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAg                        >  2:2499534/1‑140 (MQ=255)
                                                                                                             gTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAg                        >  2:1361000/1‑140 (MQ=255)
                                                                                                              tGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAgg                       >  2:13117/1‑140 (MQ=255)
                                                                                                               gATCAGCGGGCATTCCATTTATTTAAACGTAGCTACTGAGCTTCTGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGACCCCTTCCCATCTGTCAACGCCTTTCCACAAAGCGCTGCGGAAGTGACAGAAAGCGACAggg                      <  1:3262299/140‑1 (MQ=255)
                                                                                                                aTCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGt                     >  2:2896761/1‑140 (MQ=255)
                                                                                                                aTCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGt                     >  2:1660222/1‑140 (MQ=255)
                                                                                                                     cGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGACAGCGCTGAGGACGTGACAGAAAGCGACAGGGTGCTGa                >  2:2679057/1‑140 (MQ=255)
                                                                                                                        gCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAATTGACAGAAAGCGACAGGGTGCTGATGt             >  1:87441/1‑140 (MQ=255)
                                                                                                                        gCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGt             >  2:1225875/1‑140 (MQ=255)
                                                                                                                         ctttCCATTTATTTAACCGGAGCTAAGTCGGTTCAGGGCGTATTTTATGAGCCCACCGAAAGCAGCGAACCCTTCCCATCTGTAAAAGCCTTTCCCGAATGCGCTGAGGAAGTGACTGAAAGCGACAGGGTGCTGATGtt            <  2:1332712/138‑1 (MQ=255)
                                                                                                                         cATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGtt            <  2:807391/140‑1 (MQ=255)
                                                                                                                          aTTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCAGCTGTCAAAGCCTTTCCAGACAGCGCTGAGGAAGTGCCAGAAAGCGACAGGGTGCTGATGttt           >  1:178116/1‑140 (MQ=255)
                                                                                                                            tCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGTTTTg         >  1:1723861/1‑140 (MQ=255)
                                                                                                                            tCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGTTTTg         >  2:500982/1‑140 (MQ=255)
                                                                                                                              cATTTATTTAACCGGAGCTATTGAGGTTCAGGGCGTCTTTTATGAGGCCACCGACAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAACGCGCGGAGGACGGGACAGAACGCGACAGGGTGCTGATGTTTTGCt       <  1:263565/140‑1 (MQ=255)
                                                                                                                               atttatttAAACGTAGCTAATGAGCTTCTGGGCGTTTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTCCCAGAAAGCGCTGTGCAAGTCACAGAAAGCGACAGGGTGCTGATGTTTTGCTc      <  1:2189381/140‑1 (MQ=255)
                                                                                                                                tttatttaAACGGAGCTACTGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGTTTTGCTCa     <  2:2952320/140‑1 (MQ=255)
                                                                                                                                   atttaaACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGTTTTGCTCAggg  >  2:401374/1‑140 (MQ=255)
                                                                                                                                    tttaaACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTg                 <  2:1766397/124‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
TTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGTTTTGCTCAGGG  >  NZ_CP034943/3637208‑3637478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: