Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,270,0640GC26.8% 225.7 / 19.5 134L39V (CTA→GTA) prsWglutamic‑type intramembrane protease PrsW
Reads supporting (aligned to +/- strand):  ref base G (42/51);  new base C (0/34);  total (43/91)
Fisher's exact test for biased strand distribution p-value = 8.26e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 150 total aligned reads displayed.

ACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCTTTATATTC  >  NZ_CP034943/2269925‑2270203
                                                                                                                                             |                                                                                                                                           
aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                             >  1:2168438/1‑140 (MQ=255)
aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                             >  1:2125253/1‑140 (MQ=255)
aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                             >  1:520900/1‑140 (MQ=255)
aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                             >  2:2351974/1‑140 (MQ=255)
aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                             >  2:1409072/1‑140 (MQ=255)
aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                             >  2:1470841/1‑140 (MQ=255)
aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                             >  1:2540155/1‑140 (MQ=255)
aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                             >  2:519669/1‑140 (MQ=255)
aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                             >  2:1930653/1‑140 (MQ=255)
aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAACCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAAAAACCCCTAg                                                                                                                                             >  2:2186280/1‑140 (MQ=255)
aCCATCAGAATAAAACATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCAAGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCAAg                                                                                                                                             >  1:818288/1‑140 (MQ=255)
     cAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGTAACGAAAAAGCTTCCGCCTCCCACATACTCTTTTTCAAGCACGTACTGTATAAACAGAGTTGGAAATACAAGAACAACCCCTCGaaaaa                                                                                                                                        >  1:2527047/1‑140 (MQ=255)
     cAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGaaaaa                                                                                                                                        >  2:899259/1‑140 (MQ=255)
            aaCCATTTTAATTATTACTCCAAAAACCATTAATATAAAAAATAAAATAAAAAGCTTCCGCCTCCCAAATTCTATTTTTAAAGCACTTACTGGATAAAAATTGTGGCAAATAAAAGAAAAACCCCTAGAAAAAACGACCg                                                                                                                                 <  2:2444505/140‑1 (MQ=255)
              catttttAATGATCCCACCAAAAACCCTCAACATAAAAAAAAAACCAAAACGCTTCCGCCTCCCACATTCTCTTTTACAAGCACTTAGTGGAAAAACTTTGTGGTAAATACAACAACAACCCCTACAAAAAACGACCGaa                                                                                                                               <  1:1347041/137‑1 (MQ=255)
               cATTTTAATGATCCCTCAAAAAACCGTCAATATAAAAAACAAACTAAAAAGCTTCCGCCTCCCACATTCTCTTTTTAAAGCACGTACTGGATAAACTTTGTGGTAAATACAATAACACCCCCTAGAAAAAACGACCGAAg                                                                                                                              <  1:123264/140‑1 (MQ=255)
                tttttAATTTTTCCTCAAAACCCCCTCAATAAAAAAAATAAACTAAAACGCTTCCGCCGCCCAATTGCTCTTTTTCAAGCACGTACTGTAAAAACTGTGGGGTAAAAACAACAACAACCCCTACAAAAAACGACCGAAGc                                                                                                                             <  1:1409072/139‑1 (MQ=255)
                tttttAATGTTTCCTCCAAAAACCCTGAATATAAAAAATAAACGAAAAAGCTTCCGCCACCCACATTCTCTTTTTCAAGCCCGTACTGCAAAAACTTTGTGGGAAATAAAACAACACCCCCTACAAAAAACGACCGAAGc                                                                                                                             <  1:2792316/139‑1 (MQ=255)
                aTTTTAATGATTCATCCAAAAACCCTGAATATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCAAATTATCTTTTTCACGCACTTACTGGATAAAAATTGTGGTAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGc                                                                                                                             <  2:2353379/140‑1 (MQ=255)
                aTTATAATGATTCATCCAAAAACCCTGAATATAAAAAACAAACGAAACAGCTTCCGCCTCCCAAATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGTAAATACAACAACAACCCCTAGAAAAAACGACCGAAGc                                                                                                                             <  1:2224810/140‑1 (MQ=255)
                  tttATTGATTCCTCAAAAAACCCTTAATATAAAAAAGAAACTAAAAAGCTTCCGCCTCCCACTTTCTATTTTTCAAGCACGTACTGTATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCAc                                                                                                                           <  2:3029412/140‑1 (MQ=255)
                  tttAATTATTCCTCCAAAAACCATGAAGATAAAAAAGAAACCAAAACGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTTTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCAc                                                                                                                           <  2:1907861/140‑1 (MQ=255)
                  tttAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCAc                                                                                                                           >  1:2635565/1‑140 (MQ=255)
                   ttAATTTTTCCTCAAAAAACCATCAATATAAAAAATAAACGAAAAAGCTTCCGCCTCCCATATTCTCTTTTTAAAGCACTTACTGTAAAAACATTGTGGTAAATACAACAACACCCCCTACAAAAAACGACCGAAGCAcc                                                                                                                          <  1:2076351/140‑1 (MQ=255)
                   ttAATGATTCCTCAAAAAACCCTCAATAAAAAAAACAAACCAAAAAGCTTCCGCCTCCAACATTATATTTTTCAAGCACGTACGGTATAAACATTGTGGTAAATACAAGACCACCCCCTACAAAAAACGACCGAAGCAcc                                                                                                                          <  1:350444/140‑1 (MQ=255)
                   ttAATGATTCATCAAAAAACCCTGAATATAAAAAATAAACCAAAAAGCTTCCGCCTCCCACATTCTATTTTTCAAGCACGTACTGTATAAACATTGTGGTAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCAcc                                                                                                                          <  2:2830326/140‑1 (MQ=255)
                   ttAATCATTCCACAAAAAACCATAAATAAAAAAAAAAAACCAAAAAGCTTCCGCCTCCCACATTATATTTTTAAAGCACGTACTGTATAAAATTTGTGGTAAATACAACAACAACCCCTACAAAACACGACCGAAGCAcc                                                                                                                          <  1:2746874/140‑1 (MQ=255)
                     aaTTATTCCTCCAAAAACCCTGAATATAAAAAATAAACCAAAAAGGTTCCGCCTCCCACATTATCTTTTTAAAGCACGTACTGTATAAAATTTGGGGTAAATACAATAACAACCCCTAGAAAAAACGACCGAAGCACCat                                                                                                                        <  2:786921/140‑1 (MQ=255)
                     aaTGATTCCCCCAAAAACCCTTAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATACTCTTTTTCACGCACGTACTGGATAAACATTGTGGCAAATACACGAACAACCCCTAGAAAAAACGACCGAAGCACCat                                                                                                                        <  2:1504526/140‑1 (MQ=255)
                      aTGATTCCTCCAAAAACCCTTAACATAAAAAAAAAACGAAAACTCTTCCGCATCCAAAATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGTTAAATACAACACCAACCCCTAGAAAAAACGACCGAAGCACCata                                                                                                                       <  2:898816/140‑1 (MQ=255)
                         aTTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGt                                                                                                                    >  2:2897602/1‑140 (MQ=255)
                          ttCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTACAAAAAACGACCGAAGCACCATATGTa                                                                                                                   <  2:720197/140‑1 (MQ=255)
                          ttCCTCAAAACACCCTGAAGATAAAAAATAAACCACAACGCGCCCGCCTCCCACATTCTGTTTTTCAAGCACGTACTGGATAAAAATTGTGGTAAAAAAAACAACACCCCCTACAAAAAACGACCGAAGCACCATATGTa                                                                                                                   <  1:1621842/140‑1 (MQ=255)
                           tcctccAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTAc                                                                                                                  <  2:2526320/140‑1 (MQ=255)
                           tcctccAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTAc                                                                                                                  <  2:2125253/140‑1 (MQ=255)
                               ccAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGt                                                                                                              >  2:960829/1‑140 (MQ=255)
                                caaacccccTGAACATAAAAAACAAACCAAAAAGCTTCCGCCTCCCAAATTCTCTTTTTCAAGCACTTACTGCATAAACATTGTGGGAAATACAAGAACAACCCCTCCAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                             <  2:2910172/134‑1 (MQ=255)
                                cAAAACCCCCCAACATAAAAAACAAACCAAAAAGCTCCCGCCACCCACATTATCTTTTTCAAGCACGTACTGGATAAACTTGGGGGGAAATACAAGAACCCCCCCTACAAAACACGACCGAAGCACCATATGTACAGGtt                                                                                                             <  1:2177403/140‑1 (MQ=255)
                                cAAAAACCTTTAATATAAAAAAGAAACGAAAAAGCTTCCTCATCCCACATTCTCTTTTTAAAGCACGTACTGTATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                             <  2:3079915/140‑1 (MQ=255)
                                cAAAAACCCTGAACATAAAAAAAAAACCAAAAAGCTACCGCCTCCCACATTCTATTTTTCAAGAACGTACTGGATAAACATTGTGGTAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                             <  2:1049696/140‑1 (MQ=255)
                                cAAAAACCCTCACTATAAAAAATAAACCAACCTGGTCCCGCCTCCCACATCTTATTTTTCAAGCACGTACTGGATAACGTTGGTGGAAAATACACTAACAACCCCTACAAAAAACGACCGAAGCACCATATGGACAGGtt                                                                                                             <  1:2321140/140‑1 (MQ=255)
                                aaaaacCCCTCAAGATAAAAAATAAACCAAAACGCGTCCGCCTCCCACATTCTCTTTTTAAAGCCCGTACTGTATAAAATTTGTGGGAAATACAACAACACCCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                             <  1:787419/139‑1 (MQ=255)
                                aaaaaaCCCTGAATAAAAAAAATAAACCAAAAAGCTTCCGCCTCCCAATTTCTATTTTTAAAGCACGTACTGTATAAAAATTGTGGTAAATAAAACAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                             <  1:2133102/139‑1 (MQ=255)
                                aaaaaaCCATTAATAAAAAAAACAAACCAAAAAGCTTCCGCCCCCCACTTTATATTTTTAAAGCCCGTACTGGATAAACTTTGTGGTAAATAAAACAACACCCCCTACAAAACACGACCGAAGCACCATATGTACAGGtt                                                                                                             <  1:988113/139‑1 (MQ=255)
                                 aaaaaCCCATCAAGAAAAAAAAAAAACCAAAAAGCTACCCCCACCCCATTGATCTTTTTCAAGCCCGTATTGGATAAAATTTGTGGCAATTCCAACAACACCCCCACCAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                             <  1:57981/140‑1 (MQ=255)
                                   acaCCCTTAATATAAAAAATAACCCAAAAAGCGCCCGCCCCCCACATTCTCTTTTTCAAGCCCGTAGTGTATAACCTTGGTGGCAAATAAAACAACAACCCCTACAAACCACGACCGAAGCACCATATGTACAGGTTCAt                                                                                                          <  1:544401/138‑1 (MQ=255)
                                    aaCCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCAtt                                                                                                         <  2:337238/140‑1 (MQ=255)
                                       ccTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTctgtctc                                                                                                      >  2:561035/1‑134 (MQ=255)
                                       ccTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATc                                                                                                      >  2:1815791/1‑140 (MQ=255)
                                                  aaaaaGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCt                                                                                            >  1:1022354/1‑140 (MQ=255)
                                                  aaaaaGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCt                                                                                            >  1:429992/1‑140 (MQ=255)
                                                  aaaaaGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATAGGTACAGGTTCATTATCATACTGATCt                                                                                            >  1:228816/1‑140 (MQ=255)
                                                            gAAAACTCTACCGCCTCCCACATTCTCTTTTTAAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCAATAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTtaaataaa                                                                                  <  2:70180/140‑1 (MQ=255)
                                                            gAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTtaaataaa                                                                                  <  2:2635565/140‑1 (MQ=255)
                                                            cacaaaGCTTCCCCCTCCCACATTCTCTTTTTAAAGCACGTACTGCATAAACCTTGTGGGAAATACACCAACACCCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTtaaataaa                                                                                  <  1:2496383/137‑1 (MQ=255)
                                                              aaacGCTACCGCCACCCACTTTCTATTTTTAAAGCACTTACTGTATAAACATTGTGGGAAATACAACAACAACCCCAAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTaaataaaaa                                                                                <  1:3062996/140‑1 (MQ=255)
                                                              aaaaGCTTCCGCCTCCCACTTTCTCTTTTTCAAGCACGTACTGGATAAACATTTTGGTAAATAAAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTaaataaaaa                                                                                <  2:1413936/140‑1 (MQ=255)
                                                                      ccGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTa                                                                                                                   >  1:1681350/1‑97 (MQ=255)
                                                                            cccACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGAc                                                                                                                                   <  2:675427/75‑1 (MQ=255)
                                                                            cccACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGAc                                                                                                                                   >  1:675427/1‑75 (MQ=255)
                                                                            cccACATTCTATTTTTCAAGAACGTACTGTATAAACATTGTGTGAAATACAACAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAgcgc                                                                  <  1:1097197/140‑1 (MQ=255)
                                                                                        tttttCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAg                                                       >  2:2731408/1‑140 (MQ=255)
                                                                                        tttttCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAg                                                       >  2:1227300/1‑140 (MQ=255)
                                                                                         ttttCAAGAACTTACTGTATAAACATTGTGGGAAAAACAACAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGc                                                      <  1:2602850/140‑1 (MQ=255)
                                                                                           ttCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTATAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCaa                                                    >  1:1323270/1‑140 (MQ=255)
                                                                                            tCAAGAACGTCCTGGATAAACATGGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAAt                                                   <  2:1261241/140‑1 (MQ=255)
                                                                                                     tACTGGATAAACATTGTGGGAAATACAAGAACACCCCCTACAAAACACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCaga                                          <  1:2959101/140‑1 (MQ=255)
                                                                                                          gATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAgatga                                     >  2:1721468/1‑140 (MQ=255)
                                                                                                            tAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGAtt                                   >  1:653797/1‑140 (MQ=255)
                                                                                                            tAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGAtt                                   >  1:1644820/1‑140 (MQ=255)
                                                                                                              aaaaTTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGc                                 <  2:332830/137‑1 (MQ=255)
                                                                                                               ccATGGTGGAATAAACAACACCACCCCCTCTAAAACACGACCGAAGCACCTTCGGTCCAGCGTCATTATCAGACTGATCTTTTAAATAAAAATAACTTACCAGCGCGATGACGGGAGCAATTCCTGCAGAGATGATTGCa                                <  1:1455656/139‑1 (MQ=255)
                                                                                                               actttGTGGTAAATACAAGAACAACCCCTACAAAACACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCa                                <  1:2355465/137‑1 (MQ=255)
                                                                                                               aaaTTTTGGTAAATACACGAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCa                                <  1:206458/138‑1 (MQ=255)
                                                                                                               aaaTTGTGGGAAATACAACAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCa                                <  1:501711/138‑1 (MQ=255)
                                                                                                               aCATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGTTTCATTATCATTCTGATCTTTTATATAAAAATAACTTAAGAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCa                                >  2:2042543/1‑140 (MQ=255)
                                                                                                               aCATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATCAAAATAACTTAACAGCGCGAAGCCGGGAGCACTTCCTGCCGAGATGTGTGCa                                >  2:1993431/1‑140 (MQ=255)
                                                                                                                  ttGTGGTAAATACAACAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAAc                             <  1:996020/140‑1 (MQ=255)
                                                                                                                    gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt                           >  2:501249/1‑140 (MQ=255)
                                                                                                                    gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt                           >  2:2519955/1‑140 (MQ=255)
                                                                                                                    gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt                           >  1:2230769/1‑140 (MQ=255)
                                                                                                                    gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt                           >  2:1584293/1‑140 (MQ=255)
                                                                                                                         ataTACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATACCTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGc                      <  1:879866/138‑1 (MQ=255)
                                                                                                                         aaaTACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGc                      <  2:1649358/140‑1 (MQ=255)
                                                                                                                                acaacaacCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACctct               <  1:1171235/138‑1 (MQ=255)
                                                                                                                                acaacaacCCCAACAAAACACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACctct               <  1:344785/138‑1 (MQ=255)
                                                                                                                                   acaccCCCTACAAAACACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTACCAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTc            <  1:1237673/140‑1 (MQ=255)
                                                                                                                                      acCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCtt         >  2:502585/1‑140 (MQ=255)
                                                                                                                                       ccccTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAAt                                                   <  2:1147946/97‑1 (MQ=255)
                                                                                                                                       ccccTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAAt                                                   >  1:902363/1‑97 (MQ=255)
                                                                                                                                       ccccTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAAt                                                   >  1:1147946/1‑97 (MQ=255)
                                                                                                                                       ccccTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCttt        >  2:2271395/1‑140 (MQ=255)
                                                                                                                                       ccccTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCttt        >  2:3080301/1‑140 (MQ=255)
                                                                                                                                        cccTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCTTta       <  1:1584293/140‑1 (MQ=255)
                                                                                                                                        cccTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCTTta       <  1:590815/140‑1 (MQ=255)
                                                                                                                                         ccTATAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATATTTTAAATAAAAATAACTTTACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGGAACCTCTTTCCTTtat      <  2:1486522/140‑1 (MQ=255)
                                                                                                                                         ccTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCTTtat      >  2:2286381/1‑140 (MQ=255)
                                                                                                                                         ccTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCTTtat      <  2:911606/140‑1 (MQ=255)
                                                                                                                                          cTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCTTtata     <  2:1644820/140‑1 (MQ=255)
                                                                                                                                            aGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCTTTATAtt   >  2:2461668/1‑140 (MQ=255)
                                                                                                                                             |                                                                                                                                           
ACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCTTTATATTC  >  NZ_CP034943/2269925‑2270203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: