BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,991,227	0	T	G	32.8%	179.9 / 36.3	123	intergenic (+28/‑179)	gbsR/bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand): ref base T (28/54); new base G (40/0); total (68/55)
Fisher's exact test for biased strand distribution p-value = 4.86e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 131 total aligned reads displayed.

ATACGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAG  >  NZ_CP034943/2991091‑2991366

ataCGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACTGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACACAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGtttgt                                                                                                                                               >  1:994358/1‑138 (MQ=255)

ataCGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGttttt                                                                                                                                               >  1:1737219/1‑140 (MQ=255)

ataCGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAAAAAA‑AACGCCTGGGAAACCGGGCGttttt                                                                                                                                               >  1:2338632/1‑140 (MQ=255)

   cGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGACATTTTTAAATTTATACCGATTGAAACAAAGTTAAACAAA‑TACGCCTGGGACACCGGGCGttttttgt                                                                                                                                            >  1:309015/1‑138 (MQ=255)

   cGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTg                                                                                                                                            >  1:2876240/1‑140 (MQ=255)

   cGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTg                                                                                                                                            >  1:1385837/1‑140 (MQ=255)

   cGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAAAGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGtttttttt                                                                                                                                            >  1:587965/1‑139 (MQ=255)

     aGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGtt                                                                                                                                          <  2:971179/140‑1 (MQ=255)

     aGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGtt                                                                                                                                          >  2:2156822/1‑140 (MQ=255)

     aGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGtt                                                                                                                                          >  2:2858223/1‑140 (MQ=255)

      gCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑ATCGCCTGGGAAACCGGGCGTGGTTTTGttg                                                                                                                                         >  1:1931537/1‑139 (MQ=255)

      gCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGttttttttttg                                                                                                                                         >  2:1207431/1‑139 (MQ=255)

      gCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTT‑c                                                                                                                                        >  1:498750/1‑140 (MQ=255)

        aaCTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTTTT‑Gtt                                                                                                                                      >  1:1836085/1‑140 (MQ=255)

           tGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTGTTGGT‑Cttttt                                                                                                                                   >  2:2419038/1‑140 (MQ=255)

                  ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTTTT‑GTTTTTTTTTTaa                                                                                                                            >  1:2158747/1‑140 (MQ=255)

                  ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑GTTTTTTATGTaa                                                                                                                            >  1:2562428/1‑140 (MQ=255)

                  ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑GTGTTTTTTTTaa                                                                                                                            >  1:2157760/1‑140 (MQ=255)

                  ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTTTT‑TTTTTTTATGtta                                                                                                                            >  1:2315292/1‑138 (MQ=255)

                   tctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAAt                                                                                                                           <  2:77714/140‑1 (MQ=255)

                   tctAAAGCTTACTAGGCTTGGCTGAAACGGGTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAAt                                                                                                                           <  2:2315292/140‑1 (MQ=255)

                   tctAAAGATTAATATGATGGTCTGAACCGGCTGTCTCAATCGGTGCAGAGCGGTGAAATTTTTAAATTTATACCGATTCAAAAAAATTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAAt                                                                                                                           <  1:1797988/140‑1 (MQ=255)

                     tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTTTT‑GTTTTTTTTTttatta                                                                                                                         >  1:2873081/1‑140 (MQ=255)

                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTTTT‑GTTTTTTATTTAAATAATAC‑aaa                                                                                                                 >  2:1924537/1‑140 (MQ=255)

                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTTTT‑ATTTTTTATTTAATTAATAa‑aaa                                                                                                                 >  2:584730/1‑140 (MQ=255)

                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑ATTTTTTATGTAATTACTAC‑aaa                                                                                                                 >  2:2444360/1‑140 (MQ=255)

                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTGTTTTTT‑CTTTTTTTTTTAATTACaaa‑aaa                                                                                                                 >  1:1225144/1‑140 (MQ=255)

                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATAACGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTTTT‑ATTTTTTTTGTAATTAATAC‑aaa                                                                                                                 >  2:1902349/1‑140 (MQ=255)

                             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTTTT‑ATTTTTTATTTAAATAAAAC‑aaaa                                                                                                                >  2:2737643/1‑139 (MQ=255)

                               tATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AAAGCCTGGGAAACCGGGCGTTTTTTTTTT‑GGTTTTTATGTAAATACTAA‑AAA‑ttt                                                                                                             >  2:1436082/1‑140 (MQ=255)

                                   aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTTTT‑ATTTTTTATGTAATTACTAA‑AAA‑TTTTaaa                                                                                                         >  2:1091654/1‑140 (MQ=255)

                                   aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑ATTTTTTATTTAATTAATAA‑AAA‑TTTTaaa                                                                                                         >  2:1782778/1‑140 (MQ=255)

                                    ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTTTT‑GTTTTTTTTTTAATTTCTAA‑AAA‑ATTTaaaa                                                                                                        >  1:1775109/1‑140 (MQ=255)

                                    ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATTTAATTAATAA‑AAA‑tttttaaa                                                                                                        >  2:1017293/1‑135 (MQ=255)

                                    ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTTTT‑GTTTTTTATTTAATTAATAA‑AAA‑TTTTaaaa                                                                                                        >  1:3037669/1‑140 (MQ=255)

                                    ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTT‑TTTTTTTTTTTTAGTAAAAC‑AAA‑ATTTaaaa                                                                                                        >  1:1008440/1‑140 (MQ=255)

                                    ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGGGTTTGTTGTTTTTTTTTGTAA‑TGCTAC‑AAA‑ATTGtaaa                                                                                                        >  1:1925375/1‑140 (MQ=255)

                                         tcaaaCGGCTTGCTGACTCGGTGCCGAGGGGGGAAATTTTTAAATTTATACCGATTTAAACAAAGTAAAACAAA‑CACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACg                                                                                                   <  1:2542844/138‑1 (MQ=255)

                                              cGGCTTGCTGAATGGGTGCAGAGGGGTTAAATTTTTAAATTTATACCTATTTAAACAAATTAAAACAAA‑CACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑aaa                                                                                             <  1:385670/140‑1 (MQ=255)

                                                 tttgtttaaTGGGTGCAGAGCGGTTAAATTTTTAAATTTATACCTATTGAAACAATTAAAAAAAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAc                                                                                          <  1:1207431/133‑1 (MQ=255)

                                                 cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGGTTTTGTT‑TGTTTTTATGTTATTAAAACAAAA‑TTTAAAAAAAAGGGG‑aaaaa                                                                                           >  1:967528/1‑140 (MQ=255)

                                                    gCTTAATGGTTGCTGAGCGGTTTATTTTTTAAATTTTTACGGATTTAAACATATTAAAACAAA‑CACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTCCTAG‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATc                                                                                      <  1:584730/140‑1 (MQ=255)

                                                          tCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGGTTTTTTGTT‑GTTTTTTATGTTATTACTAC‑AAA‑TTTTAAAAATACGGG‑GAAAAA‑ATCCCAatt                                                                                >  2:613084/1‑140 (MQ=255)

                                                             gTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTGTTATGTAATTAATAA‑AAA‑ATTTAAAAATAAGGG‑AAAAAC‑AAACCATTTACt                                                                             >  2:2516010/1‑140 (MQ=255)

                                                                  gcgCGGGGAAATTTTTAAATTTATACCGATTGAAAAAAAGTAAAACAAA‑CACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCaa                                                                        <  1:2527910/138‑1 (MQ=255)

                                                                     cGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTT‑TTTTTTTATGTAAATAATAC‑CAA‑TTTGTCAAAAAGGGG‑AAAAAA‑ATGCCATTTTGTTACAAAat                                                                     >  2:1649765/1‑140 (MQ=255)

                                                                       gggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTTTT‑GATTTTTTTGTAATTTCGAC‑AAA‑TTTTAAAAATAAGGG‑AAAAAC‑ATGCCCTTTTCTTGCACAatat                                                                   >  2:2369865/1‑140 (MQ=255)

                                                                       gggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTT‑TTTTTTTTTTTAATTTCAAA‑AAA‑ATTGTAAAATAAGGG‑AAAAAA‑ATACCAATTACTTCCCAAatat                                                                   >  2:445251/1‑140 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑GTTTTTTATGTAATTAATAC‑AAA‑TTTGAAAAATACGGG‑AAAAAA‑AAAACATTTAATTACAAAATAtt                                                                  >  2:2560471/1‑140 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATTTAATTACTAA‑AAA‑TTTTAAAAATACGGG‑GAAAAA‑GTGCCATTTAGTTCCAAAAtttt                                                                  >  2:3048997/1‑140 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTTTT‑CTTTTTTATTTAATTAATAC‑AAA‑TATTAAAAATACGGG‑AAAAAC‑ATCCCAATTAATTCAAAAATAtt                                                                  >  2:2569753/1‑140 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTTGT‑GTTTTTTGTGTAATTTCTAC‑AAA‑GTTGGAAAAAACGGG‑AACAAA‑ATCCCAATTACTTCCAAAAtttt                                                                  >  2:923355/1‑140 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTT‑GTTTTTTTTTTAATTTCAAA‑AAA‑AATTAAAAAAAAAGG‑TAAAAC‑AAAACAATTAATTGCAAAAAAtt                                                                  >  2:2341309/1‑140 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTT‑CTTTTTTTTGTAATTAATAC‑AAA‑ATTTAAAAATACGGG‑AAAAAC‑ATACCAATTAATTGAAAAataat                                                                  >  2:338643/1‑138 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGGT‑GTTTTTTATGTTATTACTAC‑AAA‑TTTTTAAAATAAGGG‑AAAAACAAACCCATTAATTGCCaaaaatt                                                                   >  2:1094487/1‑137 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTGTTTTTT‑GTTTTTTTTGGAATTAATAC‑AAA‑TTTGAAAAATCAGGG‑CAAAAC‑AGCCCATTTACTTCCAAAatagt                                                                  >  2:1137169/1‑138 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGGTTTTTTT‑CTTTTTTATGTAATTACTAA‑AAA‑TTTGGAAAATACGGGCAAAAAA‑AACCCA‑TTAATTCCAAAAAAtt                                                                  >  2:77422/1‑140 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGGTTTTGTT‑GTTTTTTTTTTTATTTATACAAAA‑TTTTAAAAATAGGGG‑AAAAAA‑ACCCCA‑TTAGTTTCAAAAAAtt                                                                  >  1:1506098/1‑140 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACAGATTGAAACAAAGTAAAACAAA‑AACCACTGGGAAAACTGGCGTTTTTTTTTA‑GTCTTTTATTTAAATAATAA‑AAA‑TAAGTAAAAAACCGT‑CAAAAC‑CACCCATATAATTACAAAataaa                                                                  >  2:1191774/1‑138 (MQ=255)

                                                                                             gATGGAAACAAAGCAAACCAAA‑CACGCCTGCGAAACCGGGCGTATTTTTGTT‑CTTTTTTATGTAAGTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTCCTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                             <  1:2331723/140‑1 (MQ=255)

                                                                                             catacaaaCACAGTAAAACAAA‑CACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                             <  1:2052165/135‑1 (MQ=255)

                                                                                              aTTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTTTT‑TGTTTTTTTGTATATAGTAC‑AAAGTTTGAAAAAAACGGA‑CAAACA‑AGCCCATTT‑CGTAAAAAAAATTTTTTGGTAATTTTTTTTTGGTa                                            >  1:2179107/1‑140 (MQ=255)

                                                                                              aTTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTTTT‑GTTGTTTATTTAATTAAAAC‑AAA‑AATGTAAAATAAGGG‑AAAAAC‑ATAACATTTAGTTACCAAAAATTTTATGGGAATGTTATGTAGGTa                                            >  2:862871/1‑140 (MQ=255)

                                                                                              aTTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTT‑CTTTTTTATGTAATTAATAC‑CAA‑TTTTAAAAATACGGG‑AAAAAC‑ATACCAATTAATTCCAAAATATTTGATGGTAATTTTATGTAGGTa                                            >  2:2895685/1‑140 (MQ=255)

                                                                                                tGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTT‑ATTTTTTATTTAAATAATAC‑AAA‑ATTGAAAAATAAGGG‑AAAAAC‑ATACCAATTAAATACAAAAAAATTTATTGTAATTTTATTTATGTaaa                                          >  2:1166562/1‑140 (MQ=255)

                                                                                                tGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGGTGTTTTTTT‑GTTTTTTATTTAATAAAAAA‑AAA‑TATTAAAAATAACGG‑AAAAAA‑AAAACAATTACATAAAAAATATATTATTGTAATTTTtagtagcttaa                                          >  2:1841580/1‑135 (MQ=255)

                                                                                                  aaaaaaAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                        <  2:2100927/140‑1 (MQ=255)

                                                                                                  aaaCAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                        <  2:1952345/140‑1 (MQ=255)

                                                                                                  aaaCAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                        <  2:1506098/140‑1 (MQ=255)

                                                                                                       aaGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTTTT‑GTTTTTTTTTTTATTTATAC‑AAA‑ATTTTAAAATAAAGG‑AAAAAA‑AAACCAATTAAATACAAAAAAATTTTTTGTAATTTTATTTTGTTTAAAtttttt                                   >  1:1558298/1‑137 (MQ=255)

                                                                                                        aGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTGTTTGTT‑GTTTTTTATGTAATTTATAA‑AAA‑ATTGTAAAATACTGG‑CAAAAC‑ATCACAATTTATTGCAAAATATTTGTTGGTTATTTTATTGTTGTAAAAttttttt                                  >  2:1550866/1‑136 (MQ=255)

                                                                                                            aaccAAA‑CACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACTCC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                              <  2:2224377/137‑1 (MQ=255)

                                                                                                            aaacaaa‑cACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                              <  2:3037669/140‑1 (MQ=255)

                                                                                                            aaaCAAA‑ACCGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAACAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                              <  2:2575025/140‑1 (MQ=255)

                                                                                                            aaaCAAA‑AACGCCTGTGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                              <  2:1653208/140‑1 (MQ=255)

                                                                                                            aaaCAAA‑AACGCCTGGGAAACCGGGCTTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                              <  2:2790428/140‑1 (MQ=255)

                                                                                                            aaaCAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                              <  2:883360/140‑1 (MQ=255)

                                                                                                            aaaCAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                              <  1:1245469/140‑1 (MQ=255)

                                                                                                            aaaCAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                              <  2:498750/140‑1 (MQ=255)

                                                                                                            aaaCAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                              <  2:2338632/140‑1 (MQ=255)

                                                                                                            aaaCAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                              <  2:1956887/140‑1 (MQ=255)

                                                                                                             aacaaacaACCCCTGGGAAACCGGGCGTTTTTTTGTT‑CATTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                              <  1:868365/140‑1 (MQ=255)

                                                                                                                 aa‑aaCGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACTAA‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTATTTTTATTTATGTAAAATTTGTAttttggagtt                         <  2:907168/140‑8 (MQ=255)

                                                                                                                       gCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                    <  1:2156822/140‑1 (MQ=255)

                                                                                                                       gCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                    <  1:2444360/140‑1 (MQ=255)

                                                                                                                       gCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                    <  1:2737643/140‑1 (MQ=255)

                                                                                                                       cccTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATTTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                    <  1:2797778/139‑1 (MQ=255)

                                                                                                                                 ccGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAACAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaaaa          <  1:2076040/140‑1 (MQ=255)

                                                                                                                                 ccGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaaaa          <  1:2860744/140‑1 (MQ=255)

                                                                                                                                 ccGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTACCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaaaa          <  1:1597841/140‑1 (MQ=255)

                                                                                                                                      cGTTTTTTTGTT‑CTTTTTTATTTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg     <  1:1561727/140‑1 (MQ=255)

                                                                                                                                      cGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg     <  2:48814/140‑1 (MQ=255)

                                                                                                                                      cGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg     <  1:1370404/140‑1 (MQ=255)

                                                                                                                                      cGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg     <  2:1836085/140‑1 (MQ=255)

                                                                                                                                      cGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg     <  2:1512767/140‑1 (MQ=255)

                                                                                                                                      cGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg     <  1:1017293/140‑1 (MQ=255)

                                                                                                                                      cGTTTTTTTGTT‑CTTTTTTATGTAATCACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg     <  1:147348/140‑1 (MQ=255)

                                                                                                                                      cGTTTTTTTGTT‑ATTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg     <  1:2706884/140‑1 (MQ=255)

                                                                                                                                       gTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGc    <  1:1924537/140‑1 (MQ=255)

                                                                                                                                        tttttttGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCa   <  1:1782778/140‑1 (MQ=255)

ATACGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAG  >  NZ_CP034943/2991091‑2991366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`