Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,557,3000CA24.2% 377.7 / 18.3 201V886V (GTG→GTT) EO946_RS18295SpaA isopeptide‑forming pilin‑related protein
Reads supporting (aligned to +/- strand):  ref base C (62/88);  new base A (48/0);  total (113/88)
Fisher's exact test for biased strand distribution p-value = 1.66e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 204 total aligned reads displayed.

CACAGAATCGCCCGTTTTAAGGTGGACCGTCAGAGTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCACCATTATAATTGACGCCGATTTTCCACGTGATTTCCTTTGAAACTGCGTTGTATGATCCATTTTT  >  NZ_CP034943/3557162‑3557437
                                                                                                                                          |                                                                                                                                          
cacaGAATCGCCCGTTTTAAGGTGGACCGTCAGAGTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATca                                                                                                                                           <  2:2028314/140‑1 (MQ=255)
   aGAATCGCCCGTTTTAAGGTGGACCGTCAGAGTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACaa                                                                                                                                        <  2:1879541/140‑1 (MQ=255)
     aaTCGCCCGTTTTAAGGTGGACCGTCAGAGTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACg                                                                                                                                      <  2:1526410/140‑1 (MQ=255)
     aaTCGCCCGTTTTAAGGTGGACCGTCAGAGTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATAGTATATCTCTTTATAACaaag                                                                                                                                      >  2:2606768/1‑138 (MQ=255)
          cccGTTTTAAGGTGGACCGTCAGAGTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTGTTTATTGATAGTATAGGTCTTTATCACAACGGCAcc                                                                                                                                 >  1:1862735/1‑140 (MQ=255)
          cccGTTTTAAGGTGGACCGTCAGAGTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTGTTTATTAATGGTATAGCTCTTTATAAAAACGGAAcc                                                                                                                                 >  1:1496000/1‑140 (MQ=255)
          cccGTTGTAAGGTGGACCGTCAGAGTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATATCTCTTTATCACAACGGAAcc                                                                                                                                 >  2:2122773/1‑140 (MQ=255)
           ccGTTTTAAGGTGGACCGTCAGAGTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGTTCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAAccc                                                                                                                                <  1:1511219/140‑1 (MQ=255)
              ttttAAGGTGGACCGTCAGAGTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGc                                                                                                                             <  2:2807934/140‑1 (MQ=255)
               tttAAGGTGGACCGTCAGAGTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTGTTTATTAATCGGATAGCTATTTATCAAAACCGAACCCCGca                                                                                                                            >  1:1453941/1‑140 (MQ=255)
               tttAAGGTGGACCGTCAGAGTGCGTACATTTTTTTCTGAAGGCTCTTCTAACTCATACTTGGAAGGATCAATGGCGTTTCCTTCTGTGATGGAGACGTTTTTATTAAAACTATAGCTCTTTATAAAAAACGAAACCGGca                                                                                                                            >  2:1823654/1‑140 (MQ=255)
                ttAAGGTGGACCGTCAGAGTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTGATCACAACGGAACCCGGcac                                                                                                                           <  2:2701330/140‑1 (MQ=255)
                        gACCGTCAGAGTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTTATAGTTTAGCTCGTTATAAAAACGGAACCCGGCCCAAAt‑tgtt                                                                                                                  >  1:925358/1‑140 (MQ=255)
                        gACCGTCAGAGTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTGTTTTTTAATAGTATAGCTCTTTATAACAAAGGAAACCCGCACAAAa‑tgtt                                                                                                                  >  1:1187374/1‑140 (MQ=255)
                        gACCGTCAGAGTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTGTTTATTAATCGTATATCTCTTTATAACAACGGAACCCGGCACaaaaagtt                                                                                                                   >  1:2425434/1‑135 (MQ=255)
                         aCCGTCAGAGTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGGTCTTTTTCACAAAGGAACCCGGCACAAAa‑tgttg                                                                                                                 >  2:1930441/1‑140 (MQ=255)
                         aCCGTCAGAGTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAt‑tgttg                                                                                                                 <  1:969843/140‑1 (MQ=255)
                         aCCGTCAGAGTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTGTATTAATCGTATATCGATTTATAACAACGGAACCCCGCCCaaagttttt                                                                                                                  >  1:588602/1‑134 (MQ=255)
                            gTCAGAGTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAgg                                                                                                              <  1:1733870/140‑1 (MQ=255)
                             tCAGAGTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCGCGTTATAACAACGGAACCCGGCACAAAA‑TGTTGAGGa                                                                                                             >  2:2761901/1‑140 (MQ=255)
                              cAGAGTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGAt                                                                                                            <  1:362362/140‑1 (MQ=255)
                              cAGAGTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGAt                                                                                                            <  1:1221894/140‑1 (MQ=255)
                              cAGAGTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGAt                                                                                                            <  1:2600546/140‑1 (MQ=255)
                              cAGAGTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGAt                                                                                                            <  2:707325/140‑1 (MQ=255)
                              cAGAGTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACATGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGAt                                                                                                            <  2:2104635/140‑1 (MQ=255)
                              cAGAGTGCGTCCATGTGTTTCTGACGGCTCTGCGCCCCCATCCGTGGCCGGCACCCGGGCGTGTCCTTCTGTGATGGAGCCGTTGTTATTCATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGAt                                                                                                            <  2:797892/140‑1 (MQ=255)
                               agagTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATAGTATAGCTCTTTATCACAAAGGAACCCGGCACCAAA‑TGTTGAGGATc                                                                                                           >  2:588935/1‑140 (MQ=255)
                                   tGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCCCAACGGAACCCGGCCCAAAT‑GGTTGAGGATGTGtt                                                                                                       >  2:1078903/1‑140 (MQ=255)
                                     cGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATATCTGTTTATCACAACGGAACCCGGGAGCAAT‑TGTTGGGGATGTGtttt                                                                                                     >  1:675620/1‑138 (MQ=255)
                                     cGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATACCAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTAt                                                                                                     >  2:217306/1‑140 (MQ=255)
                                     cGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATAACAACGGAACCCGGCACAAAT‑TGTTTAGGTTCTGTTAt                                                                                                     >  2:1375581/1‑140 (MQ=255)
                                     cGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATAGGAAATCTCTTTATGAAAAAGGAAACCCGCAAAAAA‑TGTTGAGGATCGGTTAt                                                                                                     >  1:2283713/1‑140 (MQ=255)
                                     cGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTGTTTATTAATGGTATAGCGCTTTATAACAACGGAACCCGGCACAAAA‑TGTGGAGGATATGTTAt                                                                                                     >  2:538492/1‑140 (MQ=255)
                                       ttCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTTTTAATCGTATAGCTCTTTATAACAACCGAACCCCGCCCAAAT‑TTTTGAGGATCTGtttttg                                                                                                   >  1:1981727/1‑136 (MQ=255)
                                       ttCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATGGTATAGCTCTTTATAAAAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATgg                                                                                                   >  2:298899/1‑140 (MQ=255)
                                        tCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTCTAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGc                                                                                                  <  1:703833/140‑1 (MQ=255)
                                        tCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGc                                                                                                  <  1:2205871/140‑1 (MQ=255)
                                           tttttttCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCGTTATCACAACGGAACCCGGCACAACT‑TGGTGTGGAGCTGTTATGGCATc                                                                                               >  2:1711154/1‑140 (MQ=255)
                                            ttttttCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCt                                                                                              <  2:1325992/140‑1 (MQ=255)
                                            ttttttCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGAGGGAGCCGTTTTTATTAATCGTCTAGCTCTTTAACACAACGGAACCCGGCACAAAT‑TGTTGACGATCTGTTATGGCATCt                                                                                              <  1:444258/140‑1 (MQ=255)
                                                    gAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGAtata                                                                                      <  1:38781/140‑1 (MQ=255)
                                                        gCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGa                                                                                  <  1:1103163/140‑1 (MQ=255)
                                                         ctctTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGAt                                                                                 <  1:1238357/140‑1 (MQ=255)
                                                         ctctTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTATTTATCACAACGGAAACCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGAt                                                                                 <  1:1481687/140‑1 (MQ=255)
                                                                    cATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTTTTTATAGTATAGCTCCTTATAACAAACGAACCCCGCACAAAA‑TTTTGTTGCTCTGTTATTGGATAGTTTATATAATGATTTTTTTTGGGt                                                                      >  1:817393/1‑140 (MQ=255)
                                                                    cATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGAttttt                                                                             <  1:2029875/133‑1 (MQ=255)
                                                                    cATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGt                                                                      >  2:2118109/1‑140 (MQ=255)
                                                                    cATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAAACCGGCACAAAT‑TGTTTAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGt                                                                      >  2:2265738/1‑140 (MQ=255)
                                                                    cATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTATTTATCACAACCGAAACCCGCACAAATATGTT‑AGGAAATGTAATGGCATCTTTGATATAAGGATTTTTGTTGGGt                                                                      >  2:1497118/1‑140 (MQ=255)
                                                                    cATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCGCTTTATAACAAAGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATTGCCACGTTTATATAATGCTTTTTTGTTGGt                                                                      >  2:2707236/1‑140 (MQ=255)
                                                                     aTACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTACTCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGtt                                                                     <  1:2463769/140‑1 (MQ=255)
                                                                     aTACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGtt                                                                     <  2:1052575/140‑1 (MQ=255)
                                                                     aTACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGtt                                                                     <  1:1686343/140‑1 (MQ=255)
                                                                     aTACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGtt                                                                     <  1:1833258/140‑1 (MQ=255)
                                                                     aTACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGtt                                                                     <  1:600176/140‑1 (MQ=255)
                                                                        cTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCAc                                                                  <  1:3000803/140‑1 (MQ=255)
                                                                        cTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCAc                                                                  <  2:3036315/140‑1 (MQ=255)
                                                                        cTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCAc                                                                  <  1:805350/140‑1 (MQ=255)
                                                                        cTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCAc                                                                  <  2:1140611/140‑1 (MQ=255)
                                                                           ggAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTTTTAATAGTATATCTCTTTATCACAAACGAACCCGGCACAAAT‑TTTTGTGGATATGTTTTTGCATCTTTGATTTAAGGATTTTTTTTGGGTTCAACAt                                                               >  2:1251820/1‑140 (MQ=255)
                                                                           ggAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTTATAGTTTAGCTATTTATAACAACGGAACCCGGCAAAAAA‑TGTTGAGGTTGTGTTATGGCATCTTTGATATAAGGATTTTTTGTTGGTTAAACAt                                                               >  2:2527186/1‑140 (MQ=255)
                                                                           ggAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATATCGGTTTTTCAAAACCGAACCCGGCCAAAAA‑TGTTTATGATGTGTTATGGCATCATTGAGATAATGATTTTTTGTTGGTTCACCaa                                                               >  2:1034360/1‑139 (MQ=255)
                                                                           ggAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAAACGTATACCTAATTATAAAAAAGGAACCCGGCACAAAT‑TTTTTTAGGTGAGTTTATGCAACGTAGATATAAGGATTTTTTTTTGGTTGAACAt                                                               >  2:1174950/1‑140 (MQ=255)
                                                                           ggAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTGTTTATTAATCGTATAGCTCTTTATAACAACGGAACCCCGCACAAAT‑TGTTTAGGATCTGTTATTTCATCTTTTCTATAAGGATTTTTTTTTGGTTAACCAt                                                               >  2:814900/1‑140 (MQ=255)
                                                                           ggAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTGTTTATTAATCGGGTAGGTCTTTATAACAACGGAACCCGGGACAAAT‑TGTTGAGGATCTGTTATGTCATAGTTGATATAAGGATTTTTTTTGGGTTGAACAt                                                               >  2:464769/1‑140 (MQ=255)
                                                                             aaGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATAACAACGGAAACCGGCACAAAT‑TGTTGAGGATCTGTTATTGCATATTTTATATAAGTCTTTTTTTTTGGTTCACCATta                                                             >  2:2513665/1‑140 (MQ=255)
                                                                                gATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGGTGTTTATCACAAAGGAACCCGGCCCAAAT‑TTTTTAGGATCTGTTATGGCATCTTTTATATAAGGATTTTTTTTGGGTTGAACATTATaa                                                          >  2:366131/1‑140 (MQ=255)
                                                                                 aTCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCCCAAAT‑TGTTGAGGATCTGTTTTTGCATCTTTGATATAATGATTTTTTTTGGGTTAACCATTATAAt                                                         >  2:386977/1‑140 (MQ=255)
                                                                                 aTCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCGGTTATTGCATCGTTGATATAAGGATTTTTTGTGGGTTGAACATTATAAt                                                         >  2:2930551/1‑140 (MQ=255)
                                                                                 aTCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATAACAAAGGAACCCCGCACAAAT‑TTTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTTTGGGTTCACCATTATAAt                                                         >  2:2360927/1‑140 (MQ=255)
                                                                                 aTCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATAGTATAGCTCTTTATAAAAACGGAACCCCGCACAAAA‑TGTTTAGGATCTGTTATTGCATCTTTGATATAAAGATTTTTTGTGGGTTAACCAATATAAt                                                         >  2:1024909/1‑140 (MQ=255)
                                                                                 aTCAAGGGCGTTTCCTTCTGTGATGGAGCCGTGTTTTTTTATCGTTTAGCTCTTTATCAAAACGGAACCCGGCCCAAAA‑TGTTGTGGGTCTGTTTTTGCATCTTTTGTATAAGGATTTTTTGTGGGTTGACCagttttat                                                         >  1:2892665/1‑133 (MQ=255)
                                                                                 aTCAAGGGCGTTTCCTTCTGTGATGGAGCCGTGTTTATTTATCGTATAGCTCTTTATAAAAAACGAACCCCGCACCAAA‑TGTTTAGGATCGGTTATGTCATCTTTTATATAAGGATTTTTTTTGGGTTGACaataataat                                                         >  1:2329471/1‑140 (MQ=255)
                                                                                 aTCAAGGGCGTTTCCTTCTGTGATGGAGCCGTGTTTATTAAACGGCTAGGTCTGTATAACAAAAGAACCCGGCCCAAAT‑TTTTTAGGATCTGTTTTTGACTCTTTGATATAATGCATTTTTGTGGGTTCACAATTAAAAt                                                         >  2:970740/1‑140 (MQ=255)
                                                                                       ggCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCACCATTATAATTGACGc                                                   <  2:2071943/140‑1 (MQ=255)
                                                                                            ttCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATAACAAAGGAACCCGGCACAAAT‑TTTTGAGGATATGTTATGGGATCTTTGTTATAAGGATTTTTTTTGGGTTCACCATTATAAATTACGCCGAtt                                              >  2:1598258/1‑140 (MQ=255)
                                                                                                             ccGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCACCATTATAATTGACGCCGATTTTCCACGTGATTTCCtt                             <  2:1188336/140‑1 (MQ=255)
                                                                                                             ccGTTTTTATTAATAGTATAGCGCTTTATAACAACGGAACCCGGCACAAAT‑TGTTTAGGATCGGTTATGGCATATTTGTTATAATGATTTTTTGTTGGTTGACCAATATAATTTACGCCGATTTTACACCTGATTTCCtt                             >  2:146996/1‑140 (MQ=255)
                                                                                                               gTTTTTATTAATCGTATAGCGCTTTATCACAACGGAACCCCGCACAAAA‑AGTTGAGGAACTGTTATTGCATCTTTGATATAAGGATTTTTTGTTGGTTCACCATTATAATTGACGCCGATTTTGCACGTGAATTACGTTg                           >  2:2877944/1‑140 (MQ=255)
                                                                                                                 ttttATTAATCGTATAGCTCTTTCTCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCACCATTATAATTGACGCAGATTTTCCACGTGATTTCCTTTGaa                         <  2:420395/140‑1 (MQ=255)
                                                                                                                 ttttATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCACCATTATAATTGACGCCGATTTTCCACGTGATTTCCTTTGaa                         <  1:2118109/140‑1 (MQ=255)
                                                                                                                  tttATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCACCATTATAATTGACGCCGATTTTCCACGTGATTTCCTTTGaaa                        <  2:914179/140‑1 (MQ=255)
                                                                                                                       taATCGTATAGCTCTTTATCACAACTGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCGTTGATATAAGGATTTTTTGTGGGTTCACCATTATAATTGACGCCGATTTTCCACGTGATTTCCTTTGAAACTGCg                   <  1:399668/140‑1 (MQ=255)
                                                                                                                       taATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCACCATTATAATTGACGCCGATTTTCCACGTGATTTCCTTTGAAACTGCg                   <  1:1024767/140‑1 (MQ=255)
                                                                                                                       taATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCACCATTATAATTGACGCCGATTTTCCACGTGATTTCCTTTGAAACTGCg                   <  2:189019/140‑1 (MQ=255)
                                                                                                                       taATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCACCATTATAATTGACGCCGATTTTCCACGTGATTTCCTTTGAAACTGCg                   <  1:475651/140‑1 (MQ=255)
                                                                                                                       taATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCACCATTATAATTGACGCCGATTTTCCACGTGATTTCCTTTGAAACTGCg                   <  1:2075531/140‑1 (MQ=255)
                                                                                                                       taATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCACCATTATAATTGACGCCGATTTTCCACGTGATTTCCTTTGAAACTGCg                   <  1:2938599/140‑1 (MQ=255)
                                                                                                                               taGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCACCATTATaa                                                          <  1:1032167/93‑1 (MQ=255)
                                                                                                                               taGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCACCATTATAATTGACGCCGATTTTCCACGTGATTTCCTTTGAAACTGCGTTGTATGa           >  1:1418372/1‑140 (MQ=255)
                                                                                                                                 gCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCACCATTATAATTGACGCCGATTTTCCACGTGATTTCCTTTGAAACTGCGTTGTATGATc         <  2:1842234/140‑1 (MQ=255)
                                                                                                                                    ctTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCACCATTATAATTGACGCCGATTTTCCACGTGATGTCCTTTGAAACTGCGTTGTATGATCCAt      >  1:493855/1‑140 (MQ=255)
                                                                                                                                     tttATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCACCATTATAATTGACGCCGATTTTCCACGTGATTTCCTTTGAAACTGCGTTGTATGATCCAtt     >  2:3067876/1‑140 (MQ=255)
                                                                                                                                       tATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCACCATTATAATTGACGCCGATTTTCCACGTGATTTCCTTTGAAACTGCGTTGTATGATCCAtttt   >  1:2660713/1‑140 (MQ=255)
                                                                                                                                       tATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCACCATTATAATTGACGCCGATTTTCCACGTGATTTCCTTTGAAACTGCGTTGTATGATCCAtttt   >  2:1004176/1‑140 (MQ=255)
                                                                                                                                       tATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCACCATTATAATTGACGCCGATTTTCCACGTGATTTCCTTTGAAACTGCGTTGTATGATCCAtttt   >  1:3041330/1‑140 (MQ=255)
                                                                                                                                       tATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCACCATTATAATTGACGCCGATTTTCCACGTGATTTCCTTTGAAACTGCGTTGTATGATCCAtttt   >  1:1759176/1‑140 (MQ=255)
                                                                                                                                       tATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCACCATTATAATTGACGCCGATTTTCCACGTGATTTCCTTTGAAACTGCGTTGTATGATCCAtttt   >  2:2240018/1‑140 (MQ=255)
                                                                                                                                       tATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCACCATTATAATTGACGCCGATTTTCCACGTGATTTCCTTTGAAACTGCGTTGTATGATCCAtttt   >  2:2970875/1‑140 (MQ=255)
                                                                                                                                       tATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCACCATTATAATTGACGCCGATTTTCCACGTGATTTCCTTTGAAACTGCGTTGTATGATCCAtttt   <  1:538492/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
CACAGAATCGCCCGTTTTAAGGTGGACCGTCAGAGTGCGTTCATTTTTTTCTGAAGGCTCTTCGACCTCATACTTGGAAGGATCAAGGGCGTTTCCTTCTGTGATGGAGCCGTTTTTATTAATCGTATAGCTCTTTATCACAACGGAACCCGGCACAAAT‑TGTTGAGGATCTGTTATGGCATCTTTGATATAAGGATTTTTTGTGGGTTCACCATTATAATTGACGCCGATTTTCCACGTGATTTCCTTTGAAACTGCGTTGTATGATCCATTTTT  >  NZ_CP034943/3557162‑3557437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑