Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,838 A→G 15.4% T227T (ACA→ACG EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,8380AG15.4% 1001.5 / 151.3 398T227T (ACA→ACGEO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base A (156/176);  new base G (24/37);  total (184/214)
Fisher's exact test for biased strand distribution p-value = 3.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 416 total aligned reads displayed.

AAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACAC  >  NZ_CP034943/607699‑607977
                                                                                                                                           |                                                                                                                                           
acgtcGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACa                                                                                                                                             >  2:1839329/5‑140 (MQ=255)
acgtcGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACa                                                                                                                                             <  2:4082960/136‑1 (MQ=255)
acgtcGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGCAGCGACa                                                                                                                                             >  1:3959995/5‑140 (MQ=255)
  gtcGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAgg                                                                                                                                           >  1:2719678/3‑140 (MQ=255)
  gtcGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                                                                                                                           >  1:3390342/3‑140 (MQ=255)
  gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCTGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                                                                           >  2:1791832/1‑140 (MQ=255)
  gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                                                                           >  2:3344396/1‑140 (MQ=255)
  gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACTGGGGACGTCGGACCAGCCGGAGCCACAGCAGCTACAGGCGAAGACGGAGCAACCGGAGAGACCgg                                                                                                                                           >  2:1421791/1‑140 (MQ=17)
    cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                                                                                                                         >  2:1216264/1‑140 (MQ=255)
    cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                                                                                                                         >  1:2146115/1‑140 (MQ=255)
           gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACagg                                                                                                                                  >  1:3114727‑M2/1‑137 (MQ=255)
            ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggg                                                                                                                                 <  1:1691433‑M2/140‑5 (MQ=255)
            ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGa                                                                                                                                 <  1:405238/140‑1 (MQ=255)
                     ccggAGCTACCGGGGAACCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggc                                                                                                                        <  1:2952095/138‑1 (MQ=255)
                     cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggc                                                                                                                        >  1:3079377/1‑140 (MQ=255)
                     cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggc                                                                                                                        >  2:809083/1‑140 (MQ=255)
                      cccaccgaCCGGGGACGCCGGTCCACCGGGCCCCCCGCGAGCCACAGGCGCTACTGGGGACGCCGGCCCAGCCGGAGCGACAGGAGCTACCGGCGACGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggca                                                                                                                       <  2:2945570/133‑1 (MQ=14)
                      aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggca                                                                                                                       >  1:1208818/1‑140 (MQ=255)
                          gCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcacagg                                                                                                                   <  1:3663114/140‑1 (MQ=255)
                             aCCGGGGAAGCCGGACCAGCCGGGGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCCCAGGCCCAGgcgc                                                                                                                >  1:1492405/1‑140 (MQ=14)
                              ccGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACtggggctactggggaagtcgca                                                                                                               <  1:94722‑M2/140‑23 (MQ=255)
                              ccGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggggctactggggacgtcgga                                                                                                               <  1:2566544‑M2/140‑23 (MQ=255)
                               cGGGGACGCCCGACCAACCGGAGCGCCGGGAGCCACATCCGCTTCCGGGGACGTCGGACCAGCCGCAGCGACAGGAGCTACCGGGGAATCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagcag                                                                                                              <  1:2393068/140‑1 (MQ=21)
                               cGGGCAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGAGGGACCAGCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagcag                                                                                                              <  1:3215145/140‑1 (MQ=255)
                                   gAAGCCGGACCAGCCGGAGCGACAGGAGCTACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggggctactggggacgtcggaccagc                                                                                                          <  1:3745902‑M2/140‑28 (MQ=255)
                                   gAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGCCAGGAGCCACGGGAGCCACAGGCACAGGTGCAGCTgg                                                                                                          >  2:3422458/1‑140 (MQ=255)
                                   gAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTgg                                                                                                          >  2:1667868/1‑140 (MQ=255)
                                    aaGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTggg                                                                                                         >  2:478494/1‑140 (MQ=255)
                                    aaGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTggg                                                                                                         >  2:2037701/1‑140 (MQ=255)
                                      gCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGCCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCACCGGAGCGACGGGAGCCACaggggctacgggggacggcggaccagccgg                                                                                                       <  2:2609997‑M2/140‑31 (MQ=255)
                                        cGGACCACCAGGAGCTACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCACCCGTAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTAt                                                                                                     <  1:130789/140‑1 (MQ=21)
                                        cGGACCAACCGGCGCGCCGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGGGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACTGGCACAGGAGCAGCTGGGTTAt                                                                                                     <  2:3669107/140‑1 (MQ=21)
                                        cGGACCAACCGGAGCGACGGGAGCCACCGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggggctactggggacgtcggaccagccggag                                                                                                     <  2:917434‑M2/140‑33 (MQ=255)
                                        cGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCACCGGAGCGACGGGAGCCACaggggctcctggggacgtcggaccagccggag                                                                                                     <  2:776603‑M2/140‑33 (MQ=255)
                                        cGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggggctactggggacgtcggaccagccggag                                                                                                     <  1:1261174‑M2/140‑33 (MQ=255)
                                             cAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGaa                                                                                                <  1:1013663/140‑1 (MQ=255)
                                               cccGGCGCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCCACAGGAGCTACCGGGGAAGACGGACCAACCCGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                              <  1:1431597/139‑1 (MQ=18)
                                               cccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                              <  1:3849351/139‑1 (MQ=255)
                                               cccGGAGCGACCGGAGCCACAGCGGCTACTGGGGAAGACGGACCACCCGGAGCGACCGGAGCTACCGGGGAAGCCGCACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                              <  2:233189/139‑1 (MQ=25)
                                               aCCGGAGCGTCGGGAGCCACAGGCGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                              <  1:2825854/140‑1 (MQ=255)
                                               aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggggctactggggacgtcggaccagccggagcgacagg                                                                                              >  1:2275219‑M2/1‑101 (MQ=255)
                                               aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACgg                                                                                                                                   <  1:1113743/103‑1 (MQ=255)
                                               aCCGCACCGTCGGCAGCCACAGGGGCTACGGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACGGGGGAAGCCGGACCAACCGCAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCTGCTGGGTTATCCGAATa                                                                                              <  1:3546418/140‑1 (MQ=21)
                                                 cGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACg                                                                                            <  1:917434/140‑1 (MQ=255)
                                                    agccccGGGAGCCACAGGCGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                         <  1:1567342/135‑1 (MQ=21)
                                                    aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                         <  1:1376484/140‑1 (MQ=255)
                                                    aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                         <  1:1667868/140‑1 (MQ=255)
                                                    aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                         <  2:1691433/140‑1 (MQ=255)
                                                    aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                         <  1:3301256/140‑1 (MQ=255)
                                                    aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGCTGATCCGAAtcaggtg                                                                                         >  2:323907/1‑134 (MQ=255)
                                                      cGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGtat                                                                                       <  1:2465618/140‑1 (MQ=255)
                                                        aCGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggggctactggggacgtcggaccagccgtagcgacaggagcgaccgg                                                                                     <  1:1825114‑M2/140‑49 (MQ=255)
                                                         cGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggggctactggggacgtcggaccagccggagcgacaggagctaccggg                                                                                    <  1:2902705‑M2/140‑50 (MQ=255)
                                                         cGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggggctactggggacgtcggaccagc                                                                                                          >  1:2081653‑M2/1‑91 (MQ=255)
                                                         cGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggggctactgggcacgtcggaccagccggagcgccaggagctaccggg                                                                                    <  1:1590195‑M2/140‑50 (MQ=255)
                                                           ggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGtatatata                                                                                  >  1:1403854/1‑140 (MQ=255)
                                                            gAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGtatatatat                                                                                 <  1:478494/140‑1 (MQ=255)
                                                              gCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGcc                                                                                                                              <  1:4124444/93‑1 (MQ=255)
                                                              gCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGcc                                                                                                                              >  2:4124444/1‑93 (MQ=255)
                                                                   aGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggac                                                                          >  1:2970641‑M2/1‑81 (MQ=255)
                                                                        cTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCAc                                                                                                                                     >  2:2491403/1‑76 (MQ=255)
                                                                        cTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCAc                                                                                                                                     <  1:2491403/76‑1 (MQ=255)
                                                                        cTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggggctactggggacgtcggaccagccggagcgacaggagcta                                                                                         <  2:877051‑M2/120‑45 (MQ=255)
                                                                          aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACTGGAGCCACaggggcaactgggcaagtcgcaccagccttagcgacatgaggtacaggcgaagacgtaccaacccc                                                                   <  2:1119545‑M2/140‑67 (MQ=255)
                                                                          aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCa                                                                   >  1:523596/1‑140 (MQ=255)
                                                                             ggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGta                                                                                        <  2:3094616/116‑1 (MQ=255)
                                                                             ggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAAc                                                                <  1:809083/140‑1 (MQ=255)
                                                                             ggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAAc                                                                <  1:783519/140‑1 (MQ=255)
                                                                               ggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACgg                                                              >  2:1465073/1‑140 (MQ=255)
                                                                               ggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACgg                                                              >  1:3446708/1‑140 (MQ=255)
                                                                                gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                              <  2:284324/107‑1 (MQ=255)
                                                                                gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                             <  1:901754/140‑1 (MQ=255)
                                                                                 aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGt                                                                                                        <  2:2727952/96‑1 (MQ=255)
                                                                                 aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTa                                                            >  1:4180615/1‑140 (MQ=255)
                                                                                 aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGGATATATATAACTTAAGCGCTCAAACGGTa                                                            >  1:4019521/1‑140 (MQ=255)
                                                                                   ggcGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCCCGGGAGCCACAGGCACAGGAGCAGCTGGGTGATCCGAATACGGGTCTATATATAACTTAAGCGCTCAAACGGTAGc                                                          >  2:3795237/3‑140 (MQ=255)
                                                                                   gTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGc                                                          >  2:3834280/1‑140 (MQ=255)
                                                                                   gTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                             >  1:667681/1‑137 (MQ=255)
                                                                                   gTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTCGc                                                          >  1:3888112/1‑140 (MQ=255)
                                                                                     cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCta                                                        >  2:2431141/1‑140 (MQ=255)
                                                                                     cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCta                                                        >  2:4187561/1‑140 (MQ=255)
                                                                                     cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCta                                                        >  2:3914084/1‑140 (MQ=255)
                                                                                       gACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCtata                                                      <  1:2051697/140‑1 (MQ=255)
                                                                                        aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACgg                                                                                                                                   >  2:1330112/1‑62 (MQ=39)
                                                                                        aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATag                                                     >  2:556190/1‑140 (MQ=255)
                                                                                        aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATag                                                     >  1:2641396/1‑140 (MQ=255)
                                                                                                     acaggagcTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAgg                                                  >  2:3885510‑M2/9‑130 (MQ=255)
                                                                                                      cAGGAGCTACCGGAGAAGCCGGACCAACCGGAGCGACAGGAGCCACCGGAGCCACAGGCACACGAGCAGCTGGCGTATCCGAATACGCGTAAAGATAGACCTTAACCGCACAAACTGAAGCTAAACAGGCGGATGACAtt                                       >  2:1498244/1‑140 (MQ=19)
                                                                                                              aCCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCCACGGGAGCCACCGGCGCAGGCGCACCTGGCTTATCCGAAGCCGGGTATAGAGATACCTTAAGCGCGCAAACGGGAGCTCGCGAGGCTGATCGCATTTTTGAGTc                               >  2:3983276/1‑140 (MQ=16)
                                                                                                                  gggAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACa                           <  2:3541681/140‑1 (MQ=255)
                                                                                                                    gAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGcc                                                                                                                              <  1:379521/39‑1 (MQ=255)
                                                                                                                       gCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGAt                      >  2:2997647/1‑140 (MQ=255)
                                                                                                                         cGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAg                                                           <  2:531756/101‑1 (MQ=255)
                                                                                                                          ggACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAac                   <  1:3344197/140‑1 (MQ=255)
                                                                                                                                aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc             <  1:1845688/140‑1 (MQ=255)
                                                                                                                                 ccGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTCTATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAAACACCCCa            >  2:1922026/1‑140 (MQ=255)
                                                                                                                                   ggAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAgg          <  1:558548/140‑1 (MQ=255)
                                                                                                                                      gCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAAt       >  2:2230028/1‑140 (MQ=255)
                                                                                                                                      gCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAAt       >  2:2146108/1‑140 (MQ=255)
                                                                                                                                           aGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTacac  >  1:1976664/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACAC  >  NZ_CP034943/607699‑607977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: