Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP034943 804,480 (T)8→7 intergenic (+49/‑146) EO946_RS04095 → / → EO946_RS04100 general stress protein/pectate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943804,4730T.98.2% 812.5 / 6.1 168intergenic (+42/‑153)EO946_RS04095/EO946_RS04100general stress protein/pectate lyase
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base . (72/93);  total (73/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 176 total aligned reads displayed.

GATGAAGCCGCTCAATTCGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGGTTTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTTGTGAGAAATTGGAATATAATCTCACAAAATAGAAAA  >  NZ_CP034943/804342‑804609
                                                                                                                                   |                                                                                                                                        
gATGAAGCCGCTCAATTCGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGGtttttttgc                                                                                                                                  <  1:1890915/140‑3 (MQ=255)
gATGAAGCCGCTCAATTCGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGGtttttttgc                                                                                                                                  <  2:2435326/140‑3 (MQ=255)
gATGAAGCCGCTCAATTCGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGGtttttttgc                                                                                                                                  <  2:241587/140‑3 (MQ=255)
gATGAAGCCGCTCAATTCGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGGtttttttgc                                                                                                                                  <  1:634720/140‑3 (MQ=255)
gATGAAGCCGCTCAATTCGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGGtttttttgc                                                                                                                                  <  2:1061241/140‑3 (MQ=255)
  tGAAGCCGCTCAATTCGAACATCGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGGtttttttgcgg                                                                                                                                <  1:2066332/140‑5 (MQ=255)
  tGAAGCCGCTCAATTCGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGGtttttttgcgg                                                                                                                                <  1:1347491/140‑5 (MQ=255)
        cGCTCAATTCGAATAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAATAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTg                                                                                                                         <  2:550082/140‑1 (MQ=255)
        cGCTCAATTCGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTg                                                                                                                         <  2:2224013/140‑1 (MQ=255)
        cGCTCAATTCGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTg                                                                                                                         <  2:2497563/140‑1 (MQ=255)
        cGCTCAATTCGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTg                                                                                                                         <  2:228765/140‑1 (MQ=255)
        cGCTCAATTCGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTg                                                                                                                         <  2:2461431/140‑1 (MQ=255)
        cGCTCAATTCGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTg                                                                                                                         <  2:1085365/140‑1 (MQ=255)
        cGCTCAATTCGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTg                                                                                                                         <  2:1140239/140‑1 (MQ=255)
        cGCTCAATTCGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTg                                                                                                                         <  2:1322836/140‑1 (MQ=255)
        cGCTCAATTCGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTg                                                                                                                         <  2:1470730/140‑1 (MQ=255)
        cGCTCAATTCGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTg                                                                                                                         <  2:1596505/140‑1 (MQ=255)
        cGCTCAATTCGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTg                                                                                                                         <  2:1817065/140‑1 (MQ=255)
        cGCTCAATTCGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTg                                                                                                                         <  2:1841873/140‑1 (MQ=255)
        cGCTCAATTCGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTg                                                                                                                         <  1:1471634/140‑1 (MQ=255)
        cGCTCAATTCGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTg                                                                                                                         <  2:692616/140‑1 (MQ=255)
        cGCTCAATTCGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTg                                                                                                                         <  1:1963022/140‑1 (MQ=255)
        cGCTCAATTCGAAAAACGTTTAGATGAAGGGAAAGTGCGTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTg                                                                                                                         <  2:1451072/140‑1 (MQ=255)
        cGCTCAATTAGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGCCCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTg                                                                                                                         <  2:376384/140‑1 (MQ=255)
        cGCCCAATTCGAAAAACCTTTAGAGGAAGGGAAAGTGCTTCTCGTTGTGACCGCTAATGAAAAAGTGAAAGCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTGCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTg                                                                                                                         <  2:2163722/140‑1 (MQ=255)
          cTCAATTCGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTg                                                                                                                         <  2:2015633/138‑1 (MQ=255)
          cTCAATTCGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCg                                                                                                                       >  1:1144307/1‑140 (MQ=255)
          cTCAATTCGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGAAGGGATATAACCCAAAACGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCg                                                                                                                       >  2:1631306/1‑140 (MQ=255)
            cAATTCGAAAAAAGTTTAGATGAAGGGAAAGTTCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGgt                                                                                                                     <  2:1007482/140‑1 (MQ=255)
              aTTCGAAAAACGTTTAGATTAAGGGAAAGTGCTTCTCTTTTTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGgtgt                                                                                                                   <  2:1198418/140‑1 (MQ=255)
                 cGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTAtg                                                                                                                <  1:415566/140‑1 (MQ=255)
                           ttAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGaa                                                                                                      <  1:1358990/140‑1 (MQ=255)
                            tAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTT‑TTTTTTTTTTGTTTTATTTTCTGTGTATGTGtttctttat                                                                                                     <  2:1342398/140‑8 (MQ=255)
                              gATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGc                                                                                                   <  1:2508771/140‑1 (MQ=255)
                              gATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGc                                                                                                   <  1:527259/140‑1 (MQ=255)
                              gATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGc                                                                                                   <  1:1441380/140‑1 (MQ=255)
                                 gAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGc                                                                                                <  1:568205/140‑1 (MQ=255)
                                   aGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTg                                                                                                                         <  2:457993/113‑1 (MQ=255)
                                   aGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTg                                                                                                                         >  1:457993/1‑113 (MQ=255)
                                   aGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCaa                                                                                              <  2:2674873/140‑1 (MQ=255)
                                   aGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCaa                                                                                              <  1:2248546/140‑1 (MQ=255)
                                         aGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGAt                                                                                        >  1:1525210/1‑140 (MQ=255)
                                         aGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGAt                                                                                        >  2:657018/1‑140 (MQ=255)
                                             cTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCg                                                                                    >  1:1035039/1‑140 (MQ=255)
                                             cTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCg                                                                                    >  1:2376237/1‑140 (MQ=255)
                                             cTTCTCGTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATCCCGCAATAAGATAGCg                                                                                    >  1:2256260/1‑140 (MQ=255)
                                                ctcgTTGTGACCTATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGGATGTGTTGCAGAATGCCGCAATAAGATCGCGGaa                                                                                 >  2:21524/1‑140 (MQ=255)
                                                     tgtgACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACAttt                                                                            >  1:1819894/1‑140 (MQ=255)
                                                     tgtgACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACAttt                                                                            >  1:2488701/1‑140 (MQ=255)
                                                     tgtgACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACAttt                                                                            >  2:734331/1‑140 (MQ=255)
                                                      gtgACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACAtttt                                                                           <  1:657018/140‑1 (MQ=255)
                                                      gtgACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACAtttt                                                                           <  2:697350/140‑1 (MQ=255)
                                                      gtgACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACAtttt                                                                           <  2:737302/140‑1 (MQ=255)
                                                      gtgACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACAtttt                                                                           <  2:1668886/140‑1 (MQ=255)
                                                      gtgACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACAtttt                                                                           <  2:1986945/140‑1 (MQ=255)
                                                           cGATAATGAAACAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCACTAAGATAGCGGAACATTTTCGGtt                                                                      <  2:2692784/140‑1 (MQ=255)
                                                             agaaTGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAATATAGCGGAACATTTTCGGTTCt                                                                    >  1:1600028/3‑140 (MQ=255)
                                                             aTAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCt                                                                    >  1:1430822/1‑140 (MQ=255)
                                                             aTAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCt                                                                    >  1:1107973/1‑140 (MQ=255)
                                                                            aaTCCTGGGCATAATGCAGGGATATAACCCAACAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAAtt                                                     <  1:828529/140‑1 (MQ=255)
                                                                            aaTCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAAtt                                                     <  1:748750/140‑1 (MQ=255)
                                                                              tCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTg                                                   <  1:1194722/140‑1 (MQ=255)
                                                                              tCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTg                                                   <  2:174461/140‑1 (MQ=255)
                                                                                        aaTGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTAt                                                                                                                 <  2:1186633/68‑1 (MQ=255)
                                                                                             aGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTtgt                                    >  2:2502102/1‑140 (MQ=255)
                                                                                             aGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTtgt                                    >  2:2388430/1‑140 (MQ=255)
                                                                                             aGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGATTCTGGATGTCCCTCAATTTGCTATCATATCTTtgt                                    >  2:2626180/1‑140 (MQ=255)
                                                                                              gggATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTtgtg                                   <  2:842641/140‑1 (MQ=255)
                                                                                               ggATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGgtgt                                                                                                                   <  1:2499203/59‑1 (MQ=255)
                                                                                               ggATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTTGTga                                  >  1:2733693/1‑140 (MQ=255)
                                                                                               ggATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTTGTga                                  >  1:1210951/1‑140 (MQ=255)
                                                                                               ggATATAACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCCGTCTGTGGGGTCTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACCTTTTCGGTGCTGGATGTCCCTCAATGTGCTATCATATCTTTGTga                                  >  1:593527/1‑140 (MQ=255)
                                                                                                    taACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAAttt                                                    >  2:325966/1‑117 (MQ=255)
                                                                                                    taACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAAttt                                                    <  1:325966/117‑1 (MQ=255)
                                                                                                    taACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTTGTGAGAAAt                             >  2:1062416/1‑140 (MQ=255)
                                                                                                    taACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTTGTGAGAAAt                             <  1:2121522/140‑1 (MQ=255)
                                                                                                    taACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTTGTGAGAAAt                             >  2:1536880/1‑140 (MQ=255)
                                                                                                    taACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTTGTGAGAAAt                             >  2:2780562/1‑140 (MQ=255)
                                                                                                    taACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTTGTGAGAAAt                             >  2:2741784/1‑140 (MQ=255)
                                                                                                    taACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTTGTGAGAAAt                             >  2:1888725/1‑140 (MQ=255)
                                                                                                    taACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTTGTGAGAAAt                             >  2:2036851/1‑140 (MQ=255)
                                                                                                    taACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTTGTGAGAAAt                             >  2:2121522/1‑140 (MQ=255)
                                                                                                    taACCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTTGTGAGAAAt                             >  1:101561/1‑140 (MQ=255)
                                                                                                     aaCCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCTCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTTGTGAGAAAtt                            <  1:731269/140‑1 (MQ=255)
                                                                                                     aaCCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTTGTGAGAAAtt                            <  2:112543/140‑1 (MQ=255)
                                                                                                      aCCCAAAAGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTTGTGAGAAATTg                           <  2:543976/140‑1 (MQ=255)
                                                                                                          aaaaGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTa                                                <  1:2447252/115‑1 (MQ=255)
                                                                                                           aaaGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCg                                                                         >  1:2677461/1‑89 (MQ=255)
                                                                                                           aaaGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTTGTGAGAAATTGGAata                      >  2:2705499/1‑140 (MQ=255)
                                                                                                           aaaGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTTGTGAGAAATTGGAata                      >  1:2780069/1‑140 (MQ=255)
                                                                                                           aaaGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTTGTGAGAAATTGGAata                      >  1:2453115/1‑140 (MQ=255)
                                                                                                           aaaGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTTGTGAGAAATTGGAata                      >  2:1940192/1‑140 (MQ=255)
                                                                                                           aaaGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAAAATTTTCTGTTCTGGATGTCCCTCAATTTGATATCATATCTTTGTGAGAAATTGTAata                      >  2:315121/1‑140 (MQ=255)
                                                                                                            aaGGCCAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTTGTGAGAAATTGGAatat                     >  1:2731624/1‑140 (MQ=255)
                                                                                                                 cAACTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTTGTGAGAAATTTGAATATAAtct                >  2:2602768/1‑140 (MQ=255)
                                                                                                                 cAACTTCGGCTTTTTGGG‑‑TTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTTGTGAGAAATTGGAATATAAtctc               >  1:433575/1‑140 (MQ=255)
                                                                                                                  aaCTTCGGCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTTGTGAGAAATTGGAATATAAtctc               >  1:1492646/1‑140 (MQ=255)
                                                                                                                         gCTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTTGTGAGAAATTGTAATATAATCTCACACAAt        >  1:1759342/1‑140 (MQ=255)
                                                                                                                          cTTTTTGGG‑TTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTTGTGAGAAATTGGAATATAATCTCACAAAATa       <  2:528548/140‑1 (MQ=255)
                                                                                                                                tgtttttttttGCTGTCTTTGCGGTGTATGTGTTTCAGAATGCCGCAATAAGTTAGCTGAACATTTTCGGTTCTGGATGTACCTCAATTTGCTATCATATCTTTGTGAGAAATTGGAATATAATCTCACAAAATAGaaac  <  2:1803197/137‑2 (MQ=255)
                                                                                                                                   |                                                                                                                                        
GATGAAGCCGCTCAATTCGAAAAACGTTTAGATGAAGGGAAAGTGCTTCTCTTTGTGACCGATAATGAAAAAGTGAAATCCTGGGCATAATGCAGGGATATAACCCAAAAGGCCAACTTCGGCTTTTTGGGTTTTTTTTGCGGTCTTTGCGGTGTATGTGTTGCAGAATGCCGCAATAAGATAGCGGAACATTTTCGGTTCTGGATGTCCCTCAATTTGCTATCATATCTTTGTGAGAAATTGGAATATAATCTCACAAAATAGAAAA  >  NZ_CP034943/804342‑804609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑