Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,730,0960GC24.3% 216.0 / 15.7 117E173Q (GAA→CAA) rodZcell shape determination protein RodZ
Reads supporting (aligned to +/- strand):  ref base G (53/34);  new base C (0/28);  total (53/64)
Fisher's exact test for biased strand distribution p-value = 9.03e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 122 total aligned reads displayed.

CTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGTACGCGAT  >  NZ_CP034943/1729959‑1730230
                                                                                                                                         |                                                                                                                                      
        tCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCTACAg                                                                                                                              >  2:284792/1‑140 (MQ=255)
                gCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAAc                                                                                                                      >  1:947660/1‑140 (MQ=255)
                gCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAAc                                                                                                                      >  1:2690864/1‑140 (MQ=255)
                 cAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAATAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACt                                                                                                                     >  1:449108/1‑140 (MQ=255)
                 cAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTTATAAACt                                                                                                                     >  1:454587/1‑140 (MQ=255)
                  aaatttaaagaaagTACTAAATACCAAAATATACAACGCTAAAAGAGAATCAAAGAAACAGATCTGAAAAAGAGGCAGACACTAAAAAATAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTg                                                                                                                    <  2:1844133/134‑1 (MQ=255)
                  aaaGTCAAAGCAAGTACGAACTCCCAAAAGATTCAACGATAAAAGACAATCAAACCAACAGCTCTGAAAACGAGGCAGACACTAAAAAAGAACCGACAGAAAAAGACGATAAAAACCAAGAAAGCGACAGTGATAAACTg                                                                                                                    <  2:2182836/140‑1 (MQ=255)
                           gaaattACTAAATCCCAAAATATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAATAGGCACACACTAAAAAAAAAACGAAAGAAAAACACCATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTaaa                                                                                                           <  2:1846706/135‑1 (MQ=255)
                           gaaattACGAAATCCAAAAAGATACAACGCTAAAACACAATAAAACCAACACCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAACAAAAACAACATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTaaa                                                                                                           <  2:907089/135‑1 (MQ=255)
                           gCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTaaa                                                                                                           <  2:1888069/140‑1 (MQ=255)
                              aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCa                                                                                                        >  1:434593/1‑140 (MQ=255)
                                taataaaTCCAAAAATATACAACTCTAAAATAAAATCAAACCAACCGCTATAAAAAAGAGCCACACACAAAAAAACAAACCAAACAAAAACAACAAAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                      <  1:1888820/136‑1 (MQ=255)
                                tACTAAATCCAAAAATATTAAACTCTAAAAGCCAATAAAACCAACACCTATGAAAAAGAGGCAGACACAAAAAAAGAAACGAACCAAAAACACCAAAACAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                      <  2:449108/140‑1 (MQ=255)
                                tACTAAATCCAAAAAGATTCAACTCTAAAAGAGAATCAAAGCAACAGCTCTTAAAAAGAGGAAGAAAATAAAAAACAAACGAACGAAACAGAACATAAAAAAGAAGAACGCGACACTGATAAACTGGAGATTAAAGCAAc                                                                                                      <  2:1436435/140‑1 (MQ=255)
                                tACTAAATCAAAAAACATTAAACGATAAAATATAATAAAACAAACAGCTCTTAAAAAGAGGCAGACAATAAAAAACAAACAAAAAAAAAAGAACAAAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                      <  1:1613512/140‑1 (MQ=255)
                                tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGACGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                      >  1:2676420/1‑140 (MQ=255)
                                tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCaaa                                                                                                      >  2:484457/1‑139 (MQ=255)
                                tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                      >  1:445914/1‑140 (MQ=255)
                                tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                      >  1:1092986/1‑140 (MQ=255)
                                tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                      >  1:812957/1‑140 (MQ=255)
                                tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                      >  1:2654805/1‑140 (MQ=255)
                                tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAAAGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                      >  1:2813439/1‑140 (MQ=255)
                                    aaaTCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGc                                                                                                  >  1:96371/1‑140 (MQ=255)
                                    aaaTCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGc                                                                                                  >  1:1016336/1‑140 (MQ=255)
                                    aaaTCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGc                                                                                                  >  2:936598/1‑140 (MQ=255)
                                     aaTCCCAACAGATTCAACGATAAAAGATAATCAACGCAACAGCTCTTAAAAAGAGGAACACACTAAAAAAGAAACTAAACAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCa                                                                                                 <  2:240017/140‑1 (MQ=255)
                                          caatacaTTCAAGGATAAAATACAATCAAAGCATCAGATGTGAAAAAGAGGCAGACAGTAAAAATTAAACGAAAGAAAAATAACATAAAAATGAATAAAGCGCCAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa                                                                                            <  2:1424023/134‑1 (MQ=255)
                                          cAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa                                                                                            >  1:142463/1‑140 (MQ=255)
                                            aaaGATTAAACGCTAAAAGACAATAAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAACAAACCAAAGAAAAACAAGATAAAAAACAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAgg                                                                                          <  2:2718772/140‑1 (MQ=255)
                                             acgacccAACGATAAAACCCAATCAAAGCAACTGCTCAGAAAACCAGGCACCAACTAAAAAAGAAACTACAGAAAAAGAACCTAAACAATACCCAAGCGACAGTGATAACCTGGAGATTAAAGCAACTGGCACTGAAggg                                                                                         <  2:2600061/134‑1 (MQ=255)
                                                  tCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTACAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCAtt                                                                                    >  1:853789/1‑140 (MQ=255)
                                                   cAACGCTAAACGAGAATCAAAGAAACAGCTCTGAAAAAGAGGCATACACTAAAAAAGAAACGAAAGAAACACAACATAAAAAACACCAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTa                                                                                   <  2:1192617/140‑1 (MQ=255)
                                                     ccGCAAAAAGAGAATCAAAGCAACTGATATTAAAAATAGTCAGACACTAAAAAATAAACCAAAGAAACACAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                 <  2:1873715/139‑1 (MQ=255)
                                                     actcaaaaaCATAATCAAAGAAACAGATATAAAAAAGAGGCACACACAAAAAAAGAAACCACACAAAAAGAACATAAAAAAGAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                 <  1:1989619/135‑1 (MQ=255)
                                                     actcTAAAAGAGAATCAAACCAACATCTCTGAAAAAGACGCACACACTAAAAAAGAAACGAAAAAACAAGAACATAAATAACACGAAAGCGACAGTGATAAACGGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                 <  2:2226118/137‑1 (MQ=255)
                                                     acccaaaaaTACAATCAAAGCAACCGCTCTTAAAAACAGGCAGACACTAAAAAACAAACCAAACAAAAAGAACATAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                 <  2:2387197/135‑1 (MQ=255)
                                                     aCGCTAAACTACACTCAAAGCAACAGCTCTCAAAAAGAGGCAGACACTAAAAAACAAACGAAAGAAAAACAACATAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                 <  2:1132351/140‑1 (MQ=255)
                                                     aCGCTAAAAGAGAATCAAAGCAACCGCTCTGAAAAAGAGCCAGACACTAAAAAAGAAACGAAAGAAAAACAAGAGAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                 <  2:2223634/140‑1 (MQ=255)
                                                     aCGATAAAAGAGAATAAAAGCAACAGATCTTAAAAAGAGGACTAAAATAAAAAACAAACCAAACAAACCCAATATAAAAAAGACCACCGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                 <  1:1620772/140‑1 (MQ=255)
                                                     aCGATAAAACACAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAACGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                 <  2:560029/140‑1 (MQ=255)
                                                               acaaTCAAACCAACACCTCACAAAAATAGGAAGACACTAAAAAACAAACCAAACAAAAACAACATAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                                       <  2:947660/138‑1 (MQ=255)
                                                               acaaTAAAAGCAACAGATCTGAAAAAGAGCCAGACACTAAAAAAAAAACCAAACAAAAAGACCATAAAACAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                                       <  2:2690864/138‑1 (MQ=255)
                                                                 aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAAGTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                                     >  2:13620/1‑140 (MQ=255)
                                                                   tCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATTAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGCAGGGTCATGAACGACTTTTGTAgtgt                                                                   >  2:1654048/1‑140 (MQ=255)
                                                                   tCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgtgt                                                                   >  2:1004735/1‑140 (MQ=255)
                                                                   tCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgtgt                                                                   >  1:510974/1‑140 (MQ=255)
                                                                   caaaaGCAACAGCTCTCAAAACGCCGCAGACACTAAAAAACAAACGAAACAAAAAGAAGATAAAAAACAACACAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgtgt                                                                   <  1:1314041/138‑1 (MQ=255)
                                                                    cAAAGCAACAGCTCTTAAAAAGAGCCAGAAACTAAAAAAGAAACCAAACAAAAACAAGATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTc                                                                  <  2:434593/140‑1 (MQ=255)
                                                                    cAAAGCAACAGCTCTCAAAAATAGCCACACACTAAAAAACAAACCAAACAAAAACAAGATAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTc                                                                  <  2:1016336/140‑1 (MQ=255)
                                                                           aCAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCt                                                           >  1:1052981/1‑140 (MQ=255)
                                                                           aCAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCt                                                           >  1:29218/1‑140 (MQ=255)
                                                                               ctctGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAATGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTgaga                                                       >  2:2450191/1‑140 (MQ=255)
                                                                                   taaaaaGAGGCACACACTAAAAAACAAACCAAAAAAAAACAACATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAAtt                                                   <  1:2224841/139‑1 (MQ=255)
                                                                                   caaaaaGAGCAAGACACTAAAAAACAAACCAAACAAACATATGATAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAAtt                                                   <  1:1053537/139‑1 (MQ=255)
                                                                                    aaaaaCAGGCAGACACGAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGAGTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTc                                                  >  2:958322/1‑140 (MQ=255)
                                                                                        atagGCATACACTAAAAAACAAACGAAAGAAAAAGAAGATAAAACACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGc                                              <  2:821738/138‑1 (MQ=255)
                                                                                        agagTCAGAAACTAAAAAAGAAACGAAACAAAAAGAAGATAAAAAATAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGc                                              <  2:1538529/140‑1 (MQ=255)
                                                                                        agagGCATACAATAAAAAAGAAACGAAACAAAAAGAAGATAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGc                                              <  2:1052981/140‑1 (MQ=255)
                                                                                         gagGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATCAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                                             >  1:2649595/1‑140 (MQ=255)
                                                                                         gagGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                                             >  2:2574942/1‑140 (MQ=255)
                                                                                         gagGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                                             >  1:793065/1‑140 (MQ=255)
                                                                                         gagGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                                             >  1:2047013/1‑140 (MQ=255)
                                                                                         gagGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGAGAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                                             >  1:1491452/1‑140 (MQ=255)
                                                                                         gagGCAGACACTAAAAAACAAACGAAACAAAAACAACATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                                             <  1:2066739/140‑1 (MQ=255)
                                                                                            gCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGa                                          >  1:588979/1‑140 (MQ=255)
                                                                                            gCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGa                                          >  1:1408590/1‑140 (MQ=255)
                                                                                            gCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGACCTCGa                                          >  1:2333841/1‑140 (MQ=255)
                                                                                             cacacacAAAAAAACAACCCAAACAAAAACAAGATAAAAAACAACAACGCGACAGTGATAACCTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGaa                                         <  1:2332739/137‑1 (MQ=255)
                                                                                              aGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAAc                                        >  2:2743414/1‑140 (MQ=255)
                                                                                              aGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAAc                                        >  2:411356/1‑140 (MQ=255)
                                                                                              aGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAAc                                        >  1:2488468/1‑140 (MQ=255)
                                                                                              aGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAAc                                        >  1:1377385/1‑140 (MQ=255)
                                                                                               gACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACt                                       >  1:2236163/1‑140 (MQ=255)
                                                                                               gACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACt                                       >  1:2803014/1‑140 (MQ=255)
                                                                                                 cacTAAAAAATAAACTAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                     <  2:2731750/140‑1 (MQ=255)
                                                                                                 caatAAAAAAGAACCTAAAGAAAAACAAGATAAAAAACAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                     <  1:2747943/137‑1 (MQ=255)
                                                                                                 caatAAAAAACAAACGAAACAAAAATAATAAAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                     <  1:1804119/137‑1 (MQ=255)
                                                                                                 aacTAAATAATAAACGACAGAAAAACAATATAAAAATGAAGAAAGCTAAATTGATAAACTGGAGATTAAAGCAACTTTCACTGAAGTGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                     <  2:2509804/139‑1 (MQ=255)
                                                                                                 aaatAAAAAATAAACTAAACAAAAAGAAGATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                     <  2:2317622/137‑1 (MQ=255)
                                                                                                 aaatAAAAAATAAACCAAAGAAAAATAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                     <  2:29218/137‑1 (MQ=255)
                                                                                                 aaatAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                     <  2:2173343/137‑1 (MQ=255)
                                                                                                      acaaaGAAACGAAAGAAACCGAAGCTAAAAAAGCAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATGAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCa                                <  2:1678376/138‑1 (MQ=255)
                                                                                                           taaaCGAAATAAAAACAAGATAAAAAAGACGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                           <  2:1092986/139‑1 (MQ=255)
                                                                                                           taaaCGAAAGAAAAACAACACAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                           <  2:812957/139‑1 (MQ=255)
                                                                                                           gAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                           <  2:1408590/140‑1 (MQ=255)
                                                                                                           gAAAAGAAAGAAAAATAACATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                           <  2:2835391/140‑1 (MQ=255)
                                                                                                           caaaCTAAACAAAAAGAACATAAAACAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                           <  1:2601143/139‑1 (MQ=255)
                                                                                                           caaaCCAAATAAAAAGAACATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                           <  2:2654805/139‑1 (MQ=255)
                                                                                                            aaaCGAAAGAAAAAGAAGATAAAAAAGAAGAAACCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCTAACTGAAGGCATCAGAc                          >  2:1688648/1‑140 (MQ=255)
                                                                                                              aCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAg                        >  1:2475306/1‑140 (MQ=255)
                                                                                                              aCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTCAGACATTCGAGCTCGAACTGAAGGCATCAGACAg                        >  1:989574/1‑140 (MQ=255)
                                                                                                              aCGAAACAAAAAGAAGATAAAAAAGAAGAAAGCGACATAGATAAACTGCAGATTAAAGCAACTGTCACTGAAGGCTCATTAACGACTTATGAAGAGACCTGCGCTGAGAAATTCGAGCTCGAACTTAAGACCTCAGACAg                        >  2:1677167/1‑140 (MQ=255)
                                                                                                                 aaagaaaaagcaGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTc                     <  2:628393/140‑1 (MQ=255)
                                                                                                                      aaaagaaGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGa                <  2:510974/140‑1 (MQ=255)
                                                                                                                                  aacacaagaaAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGTAcgcg    <  2:2266433/135‑1 (MQ=255)
                                                                                                                                  aaacGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGTAcgcg    <  2:622208/140‑1 (MQ=255)
                                                                                                                                  aaaaGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGTAcgcg    <  2:2649595/140‑1 (MQ=255)
                                                                                                                                  aaaaGAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGTAcgcg    <  1:1004735/140‑1 (MQ=255)
                                                                                                                                  aaaaCAACAAAGCGACAGTGATAAACTGGAGATTAAAGCCACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGTAcgcg    <  2:622003/140‑1 (MQ=255)
                                                                                                                                    aagaagAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGTACGCGAt  <  2:1377385/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
CTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGTACGCGAT  >  NZ_CP034943/1729959‑1730230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑