Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,408,7270CA25.0% 144.5 / 14.7 88A327S (GCG→TCG) spoIIIAEstage III sporulation protein AE
Reads supporting (aligned to +/- strand):  ref base C (38/28);  new base A (0/22);  total (38/50)
Fisher's exact test for biased strand distribution p-value = 4.33e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCGGTTATAAATTTAG  >  NZ_CP034943/2408592‑2408861
                                                                                                                                       |                                                                                                                                      
cAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                                    <  1:2645561/140‑1 (MQ=255)
cAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                                    >  2:708671/1‑140 (MQ=255)
cAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                                    >  2:708636/1‑140 (MQ=255)
  aaaaTATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACa                                                                                                                                  <  2:6329/140‑1 (MQ=255)
          aaTGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCaaaa                                                                                                                          >  2:1132987/1‑140 (MQ=255)
            ttaCGCTTTTGATTATGAGTTCGTGGCAGGTAATGCGGGGCCCGCCTCCAAGGTGCTGATGAATGGGGGGTGCCAGCTTATAAATAAAAGCGAGGGAAAGAACTTTGTTTGCGGGAAAAGCCGCAATACAGATCAAAATa                                                                                                                        <  2:345155/138‑1 (MQ=255)
              aCGCTTTTGCTTATGACGTCGAGGCAGTTAATCACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGATTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCTAAATACAGATCAAAATAGc                                                                                                                      <  2:1173611/140‑1 (MQ=255)
               cGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCg                                                                                                                     >  2:2438941/1‑140 (MQ=255)
                gCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGa                                                                                                                    >  1:187596/1‑140 (MQ=255)
                gCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGa                                                                                                                    >  1:1726650/1‑140 (MQ=255)
                          ttgaCGTCGAGGCTGTTAATGCCGGGCCCGCCTCCAAGCGGCTTAATAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATGGCCGGAAAAGCCGAAATACAGATAAAAATAGCGACTCCGAGAAt                                                                                                          <  1:1951829/139‑1 (MQ=255)
                          atgaCGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGTAGAATCGCGGGTGCCATCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAGGCCGCAATACAGATCAAAATAGCGACTCCGAGAAt                                                                                                          >  1:1030559/1‑140 (MQ=255)
                          atgaCGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCTCGAAt                                                                                                          >  1:162847/1‑140 (MQ=255)
                          atgaCGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAAt                                                                                                          >  1:162282/1‑140 (MQ=255)
                          atgaCGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAAt                                                                                                          >  2:2649625/1‑140 (MQ=255)
                          atgaCGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAAt                                                                                                          >  2:2527572/1‑140 (MQ=255)
                            gaCGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATcc                                                                                                        <  2:1726650/140‑1 (MQ=255)
                             aggTCGAGGCTGTTAATGACGGGCCCGCCGCCAAGCGGCTGAAGAAGGGCGGCTGCCTGATTATAAATAAACGCGAGGTAAATCACTTTGATTGCCGCAAAAGCCTAAATACAGATAAAACTAGCGACTCCGAGAATccc                                                                                                       <  2:1722237/138‑1 (MQ=255)
                             aCGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATccc                                                                                                       <  2:301862/140‑1 (MQ=255)
                               gTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAAGCGCGGCTGCCAGCTTATAAAAAAAAGCGAGGGAAAGCACTTTGATGGCCGGAAAAGCCGAAATACCGATCAAAATAGCGACTCCGAGAATCCCTa                                                                                                     <  2:1956559/140‑1 (MQ=255)
                               gTCGAGACTGGTAATGCCGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGGGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTa                                                                                                     <  2:1384592/140‑1 (MQ=255)
                                    ggctgttAATGACGGGCCCGCCTCCAAGCGGCTGAATAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAACGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTa                                                                                                <  2:1826697/134‑1 (MQ=255)
                                    ggCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTa                                                                                                >  2:866087/1‑140 (MQ=255)
                                    ggCAGGTAATGACCGGCCCGCCACCACGCGGCTGAAGACTCGCGGCTGCCAGCTTAAAAACACCAGCGAGGGAAAGCACGTGGATGCCCGGAACAGCCGCAATAAAGATCAAAATCGCGACTCCGAGAATCCCTACTGTa                                                                                                <  2:1827446/140‑1 (MQ=255)
                                        ggTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTAtttt                                                                                            >  2:551379/1‑140 (MQ=255)
                                         ttAATGCCGGGCCCGCCTCAACGCGGATTAACAATCGGGGCTGCCAGATTATAAATAAACGCGTGGAAACGAATTTTGTTTGCCGCAAAAGCCGAAATACACAACAAAAACGCGACTCCGAGAATCCCTACTGTAttttt                                                                                           <  1:2426128/139‑1 (MQ=255)
                                         ttAATGCCGGCCCCGCCACAACGCGGCTTAAGACTCGCGGCTGCCAGATTATAAATAAACGCGAGGTAAAGCACTTTGTTTGCCGCAAAAGCCCAAAAACAGATAAAAAACGCGACTCCGAGAATCCCTACTGTAttttt                                                                                           <  1:1842733/139‑1 (MQ=255)
                                         gTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACATATCAAAATAGCGACTCCGAGAATCCCTACTGTAttttt                                                                                           <  2:2491969/140‑1 (MQ=255)
                                         gTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTAttttt                                                                                           >  2:1873789/1‑140 (MQ=255)
                                         gTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTAttttt                                                                                           >  1:2730766/1‑140 (MQ=255)
                                         gTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTAttttt                                                                                           >  1:1617069/1‑140 (MQ=255)
                                          tattGACGGGCCCGCCACCAAGCGGCTGAACAATCGCGGGTCCCAGCTAAAAAAAAAAAGCGCGGCAACCCACTTTGATTGCCGCAAACCCCGAAATACACACAAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTc                                                                                          <  1:2046323/137‑1 (MQ=255)
                                          tAATGCCGGCCCCGCCCCCACGCGGCTGAACAATCGCGGCTGCCAGATTATAAATAAACGCGAGGGAACCCACTTTGATTGCCGCAAAAGCCGCAATACAGATAAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTc                                                                                          <  1:696563/140‑1 (MQ=255)
                                          tAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACACATCAAACTAGCGACTCCGAGAATCCCTACTGTATTTTTc                                                                                          <  2:1278009/140‑1 (MQ=255)
                                            ctgccGGGCCCGCCCCCACGCGGCTGACCAAGCGCGGCTGCCAGCTTATAAATAAAAGCGCGGCAACGCACTTTGTTGGCGGCAAACCCCGAAAAACAGAACAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTcag                                                                                        <  1:1024287/136‑1 (MQ=255)
                                            aggccGGGCCCGCCTCCACGCGGCGTAAGAATCGCGGCTGCCAGCTTATAAAGAAAAGCGAGGGAAACCAGTTTGATTGCCGGAAACGCCGCAATACAGATCAAAATAGCGACTCCGAGACTCCCTACTGTATTTTTcag                                                                                        <  2:1254460/136‑1 (MQ=255)
                                            aggACGGCCCCGCCACCCCGGGGCTGAAGAAGCGCGGCTGCAAGCTTATAAAAAAACGCTCGGCAAAGAACTTTGATTGCCGGAAAAGCCTCAATCCAGATCAAACTCGCGACTCCGAGAATCCCTACTGTATTTTTcag                                                                                        <  1:1024428/138‑1 (MQ=255)
                                            aTGCCGGGCCCGCCTCCACGCGGCTGAAGAATCGCGGCTGCCAGCTTATAACTAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAACGAGCGACTCCGAGAATCCCTACTGTATTTTTcag                                                                                        <  2:139492/140‑1 (MQ=255)
                                            aTGCCGGGCCCGCCTCCAAGCGGCTGAATAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGTAATGAACTTTTATTGCCGTAAAAGCCGAATTACAGATCAAAATCGCGACTCCGAGAATCCCTACTTTATTTTTcag                                                                                        <  1:2001546/140‑1 (MQ=255)
                                            aTGACGGGCCCGCCTCCAAGCGGCGGAAGAATCGGGGCTGCCAGCTTTTAAATAAAAGCGAGGTAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTcag                                                                                        <  2:692608/140‑1 (MQ=255)
                                                   gCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAg                                                                                 >  1:712963/1‑140 (MQ=255)
                                                   gCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAg                                                                                 >  1:32412/1‑140 (MQ=255)
                                                   gCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAg                                                                                 >  2:2742958/1‑140 (MQ=255)
                                                   gCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAg                                                                                 >  1:138189/1‑140 (MQ=255)
                                                   gCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTAATAAATAAAAGCTAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCACTACAGAGCAAAATAGCGACTCCGAGAATCCCTCCTGTATTTTTCAGCAGTAAg                                                                                 >  1:2073202/1‑140 (MQ=255)
                                                    cccGCCTCCACGCGGCTTAAGACTCGGGGCTGCAAGATTAAAAATAAACCCGAGGCAAAGCACTTTGTTGGCCGTAAACGCCCAAATACACATAAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAgg                                                                                <  1:434093/140‑1 (MQ=255)
                                                        ccTCCAAGCGGCTGTAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAgc                                                                            >  1:2534736/1‑140 (MQ=255)
                                                         ctccccgCGGCTGAAGAAGCGCGGCTGCCAGCTTATAAATAACAGCGAGGGAAAGCACTTTGATTGCCGCAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAgcg                                                                           <  2:720542/134‑1 (MQ=255)
                                                         cTCCACGCGGGTTAAGAATCGCGGCGGCCAGCTTATAAAAAAACGCGAGGCAAAGCACTTTGATGGCCGGAAAAGCCGAAATACACAACAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAgcg                                                                           <  1:1873789/140‑1 (MQ=255)
                                                         cTCAAAGCGGCTTAAGAATCGCGGCTGCCTGCTTATAAATAAAAGCGAGGCAAACCACTTTGATGGCCGTAAAAGCCGAAATACAGATAAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAgcg                                                                           <  1:2490888/140‑1 (MQ=255)
                                                           ccACGCGGCTGAAGAATCGCGGCTGCAAGTTTATAAATAAAAGCGAGGGAACGCACTTTGTTTGCCGTAAACGCCGCAATACAGAACAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCt                                                                         <  1:708636/140‑1 (MQ=255)
                                                           ccACGCGGCTGAAGAATCGCGGCTCCCAGTTTATAAATAAAAGCGTGGCAACCCAGTTTTATTGCCGGAAAAGCCCAAATACATATAAAAAAAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCt                                                                         <  1:708671/140‑1 (MQ=255)
                                                              cgCGGCTGAAGACTCGCGGCTGCCAGCTTATAAATAAAACCGAGGGAAAGCACTTTGCTTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGAt                                                                      <  2:1342318/139‑1 (MQ=255)
                                                              aGCGGCTTAAGAATCGCGGCTGCCAGTTTAAAAATAAAAGCGAGGCAAACCACTTTTATTGCCGGAAAAGCCGAAAAACAGAAAAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGAt                                                                      <  1:1132987/140‑1 (MQ=255)
                                                              aGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGAt                                                                      <  2:1987713/140‑1 (MQ=255)
                                                                  gCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGAAGTAAGGAAGCGCTGATcaca                                                                  >  2:2305779/1‑140 (MQ=255)
                                                                   cTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAg                                                                 >  2:1282480/1‑140 (MQ=255)
                                                                           tCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGAACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGta                                                         >  2:1677946/1‑139 (MQ=255)
                                                                            cgcgGCTGCCAGCTTATAAATAAACGCGAGGTAAAGCACTTTGATTGCCGCAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTcg                                                        <  1:120125/140‑1 (MQ=255)
                                                                            cgcgGCTGCAAGATTATAAATAAAAGCGAGGGAAACCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTcg                                                        <  2:1305447/140‑1 (MQ=255)
                                                                             gcgGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTcgc                                                       >  2:417756/1‑140 (MQ=255)
                                                                             gcgGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTcgc                                                       >  2:1403734/1‑140 (MQ=255)
                                                                             gcgGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTcgc                                                       >  2:1314818/1‑140 (MQ=255)
                                                                                          tataAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACAtt                                          <  2:1338324/140‑1 (MQ=255)
                                                                                            taaataaaAGCGAGGTAAAGCACTTTGTTTGCCGGAAAAGCCGAAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCg                                        <  2:1826928/140‑1 (MQ=255)
                                                                                             aaataacCGCTAGGGAAAGCATTTTTTTTGCCGTAAAAGCCTCAATACAGATAAAATTAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCgg                                       <  1:2516820/140‑1 (MQ=255)
                                                                                               atacacgCGAGGGAAAGCACTTTGATTGCCGCAAAAGCCGAAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                     <  2:2451030/134‑1 (MQ=255)
                                                                                               ataacAGCGAGGCAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAACTAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                     <  2:2129359/140‑1 (MQ=255)
                                                                                               ataaaTGCTAGGCAAATCAGTTTGATTGCCGCAAAAGCCGAAATACACATCAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                     <  2:251075/140‑1 (MQ=255)
                                                                                               ataaaCGCGAGGCAAAGCACTTTGTTTGCCGGAAAAGCCGAAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                     <  1:2438941/140‑1 (MQ=255)
                                                                                               ataaaAGCGTGGTAAAGCACTTTTATTGCGGGAAAAGCCGAAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                     <  1:741809/140‑1 (MQ=255)
                                                                                               ataaaAGCGTGGGAAAGCACTTTGATTGCCGGAAAAGCCGAAATAAAGATAAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                     <  2:521193/140‑1 (MQ=255)
                                                                                               ataaaAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                     <  2:2207291/140‑1 (MQ=255)
                                                                                               ataaaAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGAAATAAAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                     <  2:1197210/140‑1 (MQ=255)
                                                                                                   aaGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCt                                                                         <  2:1459212/100‑1 (MQ=255)
                                                                                                   aaGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCt                                                                         >  1:1459212/1‑100 (MQ=255)
                                                                                                   aaGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCTCGTCTGTAAACATTCGGCCAAGt                                 >  2:1381587/1‑140 (MQ=255)
                                                                                                   aaGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGt                                 >  2:1757308/1‑140 (MQ=255)
                                                                                                   aaGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGt                                 >  2:1728368/1‑140 (MQ=255)
                                                                                                        aGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAgg                            >  1:241697/1‑140 (MQ=255)
                                                                                                           taaaGCACTTTGTTTGCCGGAAAAGCCGAAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTTTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAAt                         <  2:1768612/139‑1 (MQ=255)
                                                                                                                   tttGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTc                 >  1:1530300/1‑140 (MQ=255)
                                                                                                                   tttGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTc                 >  1:1355508/1‑140 (MQ=255)
                                                                                                                     tGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATa                        >  1:556707/1‑131 (MQ=255)
                                                                                                                     tGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATa                        <  2:556707/131‑1 (MQ=255)
                                                                                                                          gCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCGGTtat          >  1:2593327/1‑140 (MQ=255)
                                                                                                                          gCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTAATGTAATTTTCAGCAGTAAGTAATCGCTGATCACAGTCTCTGTCGCGTATGTAAACATTCGTCCAAGTACAGGAATAAAGTTTCCTGGtat          >  2:294625/1‑140 (MQ=255)
                                                                                                                                  cgCCGCATTACACTTCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCGGTTATAAATTTAg  <  2:1223119/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCGGTTATAAATTTAG  >  NZ_CP034943/2408592‑2408861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: