Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,539,5920CG25.6% 252.3 / 14.7 130E121Q (GAA→CAA) EO946_RS18215T7SS effector LXG polymorphic toxin
Reads supporting (aligned to +/- strand):  ref base C (26/61);  new base G (30/0);  total (69/61)
Fisher's exact test for biased strand distribution p-value = 9.36e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 138 total aligned reads displayed.

GATTCATCAAGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAAT  >  NZ_CP034943/3539453‑3539730
                                                                                                                                           |                                                                                                                                          
gATTCATCAAGCTTCTCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATttc                                                                                                                                            <  2:405144/140‑1 (MQ=255)
gATTCATCAAGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATttc                                                                                                                                            <  2:1116122/140‑1 (MQ=255)
gATTCATCAAGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATttc                                                                                                                                            <  2:1574036/140‑1 (MQ=255)
  ttCATCAAGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATttctt                                                                                                                                          >  1:892490/1‑140 (MQ=255)
  ttCATCAAGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATttctt                                                                                                                                          >  2:1977527/1‑140 (MQ=255)
  ttCATCAAGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATttctt                                                                                                                                          >  2:1976181/1‑140 (MQ=255)
         aGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTGTTTATg                                                                                                                                   >  1:1974893/1‑140 (MQ=255)
         aGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATg                                                                                                                                   >  1:2800051/1‑140 (MQ=255)
         aGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATg                                                                                                                                   >  1:1986988/1‑140 (MQ=255)
          gCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTAtta                                                                                                                                  >  1:78575/1‑138 (MQ=255)
             tATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCg                                                                                                                               >  2:288099/1‑140 (MQ=255)
             tATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTATTATTTATGATCg                                                                                                                               >  2:2307275/1‑140 (MQ=255)
               tCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCCTCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTGTTTATGATCGga                                                                                                                             >  2:266933/1‑140 (MQ=255)
               tCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGga                                                                                                                             >  2:2023297/1‑140 (MQ=255)
               tCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGga                                                                                                                             <  1:2014914/140‑1 (MQ=255)
                  aCTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGAc                                                                                                                          <  2:525734/140‑1 (MQ=255)
                    tgctgTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTGTTGTTTTTGAGCGGAGACaa                                                                                                                        >  1:1874450/1‑140 (MQ=255)
                      ctgTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAAtt                                                                                                                      >  2:857761/1‑140 (MQ=255)
                      ctgTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTATTAGTTATGAGAGGAGACAAtt                                                                                                                      >  2:1188577/1‑140 (MQ=255)
                        gtgATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAAatta                                                                                                                    >  2:521704/1‑139 (MQ=255)
                          gATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTg                                                                                                                  <  2:2109258/140‑1 (MQ=255)
                            ttGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAg                                                                                                                <  2:2197897/140‑1 (MQ=255)
                                gtCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTGTTTATTAGCCGGGACAAATTCTGAGGCtt                                                                                                            >  1:2503686/1‑140 (MQ=255)
                                gtCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTGTTCTTTATGAGCGGAGACAATTTCTGAGGcgt                                                                                                            >  2:427293/1‑138 (MQ=255)
                                gtCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTATTATTTATGAGCGGCGACAAATTCTTAGGCtt                                                                                                            >  2:207768/1‑140 (MQ=255)
                                 tCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTGTTTATGAGCGGAGCCAAATTCTGAGGCttt                                                                                                           >  1:911422/1‑140 (MQ=255)
                                    cGTATGATTTTTTGGGCATCGACAATTTTATGGTCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGTAGTCGATTTCTTCTTTATGAGCGGATACAATTTCTGAGGCTTTCaa                                                                                                        <  2:2644135/140‑1 (MQ=255)
                                     gTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATATTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCTATGTGTTCTTTTTGAGGGGAGAGAAATTGTGGGGGTTTGAAg                                                                                                       >  1:1798894/1‑140 (MQ=255)
                                      tCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGt                                                                                                      <  2:2214350/140‑1 (MQ=255)
                                      tCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGt                                                                                                      <  1:613789/140‑1 (MQ=255)
                                      tCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGt                                                                                                      <  2:439722/140‑1 (MQ=255)
                                      tCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGt                                                                                                      <  1:1041263/140‑1 (MQ=255)
                                      tCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGt                                                                                                      <  1:1678026/140‑1 (MQ=255)
                                      tCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGt                                                                                                      <  1:1677637/140‑1 (MQ=255)
                                      tCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGt                                                                                                      <  1:2440066/140‑1 (MQ=255)
                                      tCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAACAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGt                                                                                                      <  2:533720/140‑1 (MQ=255)
                                      tCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACAGGTCGAGATCAATTATCTCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCGGAGGCTTTCAAGt                                                                                                      <  1:1677725/140‑1 (MQ=255)
                                           ttttttGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCa                                                                                                 <  2:1986988/140‑1 (MQ=255)
                                                tGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTggg                                                                                            <  1:1620183/140‑1 (MQ=255)
                                                            aTCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATc                                                                                <  1:2106306/140‑1 (MQ=255)
                                                                 aTCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTATTCTTTATTAGCGGAGACAAATTGTGAGGCTTTAAATTGTCCATTGGGCCGCTCATGAGCCcaaa                                                                           >  1:1537677/1‑140 (MQ=255)
                                                                  tCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTGTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATGCcaaaa                                                                          >  1:601190/1‑140 (MQ=255)
                                                                      cATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAaagaa                                                                      <  2:1170484/140‑1 (MQ=255)
                                                                           tcgtcAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTc                                                                 <  1:2041176/140‑1 (MQ=255)
                                                                           tcgtcAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTc                                                                 <  1:705198/140‑1 (MQ=255)
                                                                                aaGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCGTTATTTGCGGTGACAATTTATGAGGCTTTGAAGTCTCCATTGGGCAGCTCATGATCCCAAAAATAACTTTCCACat                                                            >  1:2303099/1‑140 (MQ=255)
                                                                                aaGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTGTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGGAGCTCATGATCCAAAAAAGAAGTTTACACat                                                            >  2:1027876/1‑140 (MQ=255)
                                                                                aaGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGt                                                                                                      >  2:1445571/1‑98 (MQ=255)
                                                                                aaGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGt                                                                                                      <  1:1445571/98‑1 (MQ=255)
                                                                                aaGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACat                                                            <  1:2520569/140‑1 (MQ=255)
                                                                                aaGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATACAAAAAAGAAGTTTCCAAat                                                            >  2:2015272/1‑140 (MQ=255)
                                                                                     tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTGTTTTTTAGCGGAGACCATTTGAGAGGCTTTGAAGTCTCCAATGGGCAGCACATGATCCAAAAAAGAAGTTTGCACAGATGaa                                                       >  1:4869/1‑140 (MQ=255)
                                                                                     tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTGTTTATGAGCGGCGACAAATTCTGAGGCTTTCAAGTCTGCATTGGGGAGCACATGATCCAAAAAATAACTTTCCACATATGaa                                                       >  2:2791810/1‑140 (MQ=255)
                                                                                     tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTGTTTATGAGCGGAGACAATTTCTGAGGCTTTGAAGTCTACATTGGGCAGCTCATTTTCCAAAAAAGAAGTTTCCACAAATTaa                                                       >  2:1047772/1‑140 (MQ=255)
                                                                                     tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTATTATCGGAGACAAATTATGAGGCTTTCAAATATCCAATTGGCAGCTCAATATACAAAAAAAAAATTTGCACAAATTaa                                                       >  1:449328/1‑140 (MQ=255)
                                                                                     tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATACAAAAAAGAAATTTTcacaaaatga                                                       >  2:2788509/1‑134 (MQ=255)
                                                                                     tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTAAAGTCGACATTGGGCAGCTCAGTATCCAAAAAATAAGTTTCCACATATGaa                                                       >  1:2699876/1‑140 (MQ=255)
                                                                                     tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTGTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGaa                                                       >  2:60885/1‑140 (MQ=255)
                                                                                     tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGTGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGaa                                                       >  2:1500517/1‑140 (MQ=255)
                                                                                     tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGaa                                                       <  2:1332006/140‑1 (MQ=255)
                                                                                     tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGaa                                                       <  1:262568/140‑1 (MQ=255)
                                                                                     tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGaa                                                       <  2:324737/140‑1 (MQ=255)
                                                                                     tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGaa                                                       <  2:1332035/140‑1 (MQ=255)
                                                                                     tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTATTGTTTATTAGCGGAGACCAATTATGGGGCTTTCAATTCTCCAATGGGCAGCTCAAGATCCAAAAAAAAAGATTACACAAAAGaa                                                       >  1:1880902/1‑140 (MQ=255)
                                                                                     tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTATTGTATATGTGACCACAAAATATCTGAGGCATTCTACTATAAAAAGGGCCGCTCATGATTCAAAAAAAAATTTTTCACATATTaa                                                       >  1:1835938/1‑140 (MQ=255)
                                                                                     tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTGTTGTTTATGTGCGGCGACAAATTGGGAGGCTTTGAAGTGTCCCTTTGGCAGCTCATGATACAAAAAAGAAGTTTGCACataataa                                                       >  1:1564617/1‑136 (MQ=255)
                                                                                       tACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTATGAGCGGAGAAAAATTCTTAGGCTTTGAAGTCTCCATTGGGCAGCTCATTTTACAAAAAAAAAGTTTCCACAAATGAAta                                                     >  1:1922669/1‑139 (MQ=255)
                                                                                         cATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCC‑AAAAAGAAGTTTCCACATATGAATCAtt                                                  <  2:2453188/140‑1 (MQ=255)
                                                                                          aTGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCAtt                                                  <  1:1976181/140‑1 (MQ=255)
                                                                                          aTGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCC‑AAAAAGAAGTTTCCACATATGAATCATTc                                                 <  1:412198/140‑1 (MQ=255)
                                                                                           tGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTc                                                 <  1:842949/140‑1 (MQ=255)
                                                                                            gTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTGTTTTTGATCGGAGGCAATTTCTGAGGGTTTGAAGTGTCCATTGGGGAGCTCATTATCCAAAAAAGAAATTTGCACCTATTAATCaatga                                                >  1:1883796/1‑136 (MQ=255)
                                                                                            gTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTGATTTATGAGCGGAGACAATTTCTGTGGCGTTGAAGTCGCCAGTGGGCAGCTCATGTTCCCAAAAAGTAGTTTCACCACATAAATCAttgc                                                >  1:1371459/1‑138 (MQ=255)
                                                                                            gTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTAGGAGCGGAGACCATTTCTGAGGGTTTCAAGTCTCCAATGGGCCGCTCATGATCCAAAAAAGAAGTGTCCACATATGaagaatggg                                                >  2:1461941/1‑133 (MQ=255)
                                                                                            gTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTATTTATTAGCGGAGACAATTTATGAGGCTTTCAAGTATCCAATGGGCAGCTCATTATCCAAAAAAGAAGTTTACACATATTAATCATTCa                                                >  2:919960/1‑140 (MQ=255)
                                                                                            gTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTATTCTTTATGATCGGCGACAAATTCTGAGGCTTTCAACTATCCAATTGGCAGCTCATTATACAAAAAAGAAGTTTGCACATATGAATAAttga                                                >  2:1096137/1‑138 (MQ=255)
                                                                                            gTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTATTCTTTATGAGCGGATACAATTTCTGAGGCTTTAAATTCTCAATTGGGCAGCTCATGATCCAAAAAAGAACTTTACACAAATGAATCATTCa                                                >  2:579047/1‑140 (MQ=255)
                                                                                            gTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTATTCTTTATGAGCGGAGACCAGTTATGTGGCTTTGAAGTCTCCATTGGGCAGCACATGTTCCAAAAAATAAGTTTACACATATGAAtaaataa                                                >  2:1134282/1‑134 (MQ=255)
                                                                                            gTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTGTTCTTTATTTTCGGAGACAAGTTCTGATGCTTTCAAGTCTCCATTTGGCCGCGCATGATCCAAAAAATAAGTTTGCACAAATGTATCATTCa                                                >  1:1371488/1‑140 (MQ=255)
                                                                                            gTCGAGATCAATAATATCGCTCATGCCTGACAAAATGGAGTGGAGTTGTTCTTTATGACCGGAGACCATATATGAAGCATTCAAGTCTCCAATGGGCAGCGCAATATGACAAAAACAAGATTCCCCCAATGAATAAttta                                                >  2:1134372/1‑138 (MQ=255)
                                                                                               gagaTCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTATTTATGATCGGAGACAATTTCTGATGCTTTCAAGTATCCATTGGGCAGCTCATTATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGtt                                             >  2:794134/1‑140 (MQ=255)
                                                                                               gagaTCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTATTGTTTAATTTCGGAGAAAATTTATTATGCTTTAAATACTACAATTGGCATCACAAGATACAAAAAAAAAATTTCCACAAATTAATCAttaattt                                             >  1:2047464/1‑135 (MQ=255)
                                                                                                  aTCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCtt                                          <  1:1047772/140‑1 (MQ=255)
                                                                                                  aTCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCtt                                          <  1:1100186/140‑1 (MQ=255)
                                                                                                   tCAATAATATCGCTGATGCCTGACAAAATGGTGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTg                                         <  1:2721064/140‑1 (MQ=255)
                                                                                                   tCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTg                                         <  1:1229207/140‑1 (MQ=255)
                                                                                                   tCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTg                                         <  2:1962506/140‑1 (MQ=255)
                                                                                                   tCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTg                                         <  1:33890/140‑1 (MQ=255)
                                                                                                   ccactcatatCGCGGACGCCTGACAAACTCGAGTCGATTTCCTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCCAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTg                                         <  1:1461941/134‑1 (MQ=255)
                                                                                                       taataTCGCTGATGCCTGACAAAATGGAGTCGATTTCTTGTTTATTATCGGAGACAATTTGTGAGGCTTTCAAGTCGCCATTTGGCAGCTCATGTTACAAAAAAGAACGTTCCCCAAATGAAACATTAAGTTGGTGATCt                                     >  2:1201419/1‑140 (MQ=255)
                                                                                                          tatCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTa                                  <  1:2349245/140‑1 (MQ=255)
                                                                                                            tCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAAt                                <  2:1798894/140‑1 (MQ=255)
                                                                                                             cGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTTGGCAGCTCATGATCCAAAAAAGAAGTTTACACATATGAATCATTCAGTTATTTATATTTAAtt                               >  2:2297922/1‑139 (MQ=255)
                                                                                                             cGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCGCATGATCCAAAAAACAAGTTTACACATATGAATCATTCAGTTATTTATATTTaaag                               >  2:2795749/1‑138 (MQ=255)
                                                                                                             cGCTGATGCCTGACAAAATGGAGTCGATTTATTGTTTATGAGCGGCGACAAATTCTGAGGCTTTCAAGTCTACATTTGGCAGCTCATGATACACAAAAGAATTTTACACAAATGAATCATTGAGTTGTTTTTATTTAATg                               >  2:200653/1‑140 (MQ=255)
                                                                                                               cTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTTGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATTAATCATTAAGTTCTTGATATTTAATGtc                             >  2:1971402/1‑140 (MQ=255)
                                                                                                               cTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGtc                             <  2:82798/140‑1 (MQ=255)
                                                                                                                         aCAAAATGGAGTCGATTTGTTCTTTATTAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCAATGGGCAGCTCATTTTCCCAAAAAGAAGTTTACACATATGAATCATTCAGTTATTGATCTTTAATGTCTCCATAAAtt                   >  2:920367/1‑139 (MQ=255)
                                                                                                                            aaaTGGAGTCGATTTGTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAATTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAATTTACACATATGAATCATTAAGTTGTTGATCTTTAATTTATACTGAAATTCCa                >  2:1743021/1‑140 (MQ=255)
                                                                                                                                  aGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCAATTGGCAGCTCATGATCCAAAAAAGAATTTTCCACATATGAATCATTAAGTTCTTGATCTTTAATTTCTACTGAAATTCCCTTTaaa          >  2:2198996/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
GATTCATCAAGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAAT  >  NZ_CP034943/3539453‑3539730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑