BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP034943	132,152	G→A	E549K (GAA→AAA)	fusA →	elongation factor G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	132,152	0	G	A	99.2%	968.1 / NA	255	E549K (GAA→AAA)	fusA	elongation factor G
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (147/106); minor base C (2/0); total (149/106)
Fisher's exact test for biased strand distribution p-value = 5.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 259 total aligned reads displayed.

TCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAA  >  NZ_CP034943/132016‑132288

tCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTaaaa                                                                                                                                       >  2:1505019/1‑140 (MQ=255)

   gTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATg                                                                                                                                    <  2:1102233/140‑1 (MQ=255)

   gTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATg                                                                                                                                    >  1:385467/1‑140 (MQ=255)

   gTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATg                                                                                                                                    >  1:1201231/1‑140 (MQ=255)

   gTTCGGACACGTTTGGAGCGAATGCGAACCAAACGAAGAAGGCGCTTGCTTTGAAGTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCGAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATg                                                                                                                                    <  2:2466176/140‑1 (MQ=255)

             gTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGc                                                                                                                          <  1:1023088/140‑1 (MQ=255)

              tttGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGGACTTAAAAATGGTGTACTGGCa                                                                                                                         >  2:530/1‑140 (MQ=255)

              tttGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTaaaa                                                                                                                                       <  1:1958187/126‑1 (MQ=255)

              tttGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCa                                                                                                                         >  2:954122/1‑140 (MQ=255)

              tttGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCa                                                                                                                         >  1:275185/1‑140 (MQ=255)

              tttGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCa                                                                                                                         >  2:2070446/1‑140 (MQ=255)

                tGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGc                                                                                                                          >  1:43879/1‑137 (MQ=255)

                     cGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTcc                                                                                                                  <  1:1528725/140‑1 (MQ=255)

                       aaTTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGc                                                                                                                          >  1:2125342/1‑130 (MQ=255)

                       aaTTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCg                                                                                                                >  2:684730/1‑140 (MQ=255)

                       aaTTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCg                                                                                                                >  1:696127/1‑140 (MQ=255)

                       aaTTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCg                                                                                                                >  1:1108251/1‑140 (MQ=255)

                       aaTTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCg                                                                                                                >  1:2645238/1‑140 (MQ=255)

                             aaCCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATc                                                                                                          <  2:2021975/140‑1 (MQ=255)

                                  aaCGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACAt                                                                                                     <  2:1813442/140‑1 (MQ=255)

                                       agaagGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAg                                                                                                >  2:353161/1‑140 (MQ=255)

                                          agGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCaa                                                                                             <  1:1425490/140‑1 (MQ=255)

                                             cgcTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTcc                                                                                                                  >  2:2674538/1‑116 (MQ=255)

                                             cgcTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAAc                                                                                          <  2:785878/140‑1 (MQ=255)

                                             cgcTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAAc                                                                                          >  2:1576056/1‑140 (MQ=255)

                                               cttgcttTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACtt                                                                                        <  2:1658055/140‑1 (MQ=255)

                                               cttgcttTGAATTTGAAAATGCGATTGTCGGTGGGGTAGGTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACtt                                                                                        <  1:2111462/140‑1 (MQ=255)

                                                   cttTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAATATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCg                                                                                    <  2:2346651/140‑1 (MQ=255)

                                                    tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                                                                                   <  1:1919929/140‑1 (MQ=255)

                                                    tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                                                                                   <  2:562371/140‑1 (MQ=255)

                                                    tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                                                                                   <  2:1680490/140‑1 (MQ=255)

                                                    tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                                                                                   <  1:2516354/140‑1 (MQ=255)

                                                    tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                                                                                   <  2:1865050/140‑1 (MQ=255)

                                                    tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                                                                                   <  2:2043147/140‑1 (MQ=255)

                                                    tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                                                                                   <  1:2028849/140‑1 (MQ=255)

                                                    tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                                                                                   <  2:2434589/140‑1 (MQ=255)

                                                    tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                                                                                   <  1:1906879/140‑1 (MQ=255)

                                                     ttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGAt                                                                                  <  2:1587878/140‑1 (MQ=255)

                                                      tgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCATGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATg                                                                                 >  2:132929/1‑140 (MQ=255)

                                                         atttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGtt                                                                              <  1:1552113/140‑1 (MQ=255)

                                                           ttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCt                                                                            <  1:720337/140‑1 (MQ=255)

                                                             gaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                                                                                   <  2:939470/131‑1 (MQ=255)

                                                                  tGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTCAACATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTACGATGGTTCTTACCatg                                                                     >  1:1654991/1‑140 (MQ=255)

                                                                  tGCGATAGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCatg                                                                     >  1:1069537/1‑140 (MQ=255)

                                                                     gtttGTCGGTGGTGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCatgatg                                                                  <  2:2116251/138‑1 (MQ=255)

                                                                      aTTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGt                                                                 <  2:1201231/140‑1 (MQ=255)

                                                                                    aGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGaaa                                                   >  1:893187/1‑140 (MQ=255)

                                                                                    aGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGaaa                                                   >  1:1743582/1‑140 (MQ=255)

                                                                                        ccACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGc                                               >  1:128583/1‑140 (MQ=255)

                                                                                           cGTGAATACCTCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                            <  1:591391/140‑1 (MQ=255)

                                                                                           cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                            >  1:1875331/1‑140 (MQ=255)

                                                                                           cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                            >  1:2555349/1‑140 (MQ=255)

                                                                                           cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                            >  2:2590634/1‑140 (MQ=255)

                                                                                           cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                            >  2:2769852/1‑140 (MQ=255)

                                                                                           cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                            >  2:1361271/1‑140 (MQ=255)

                                                                                           cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                            <  1:1998518/140‑1 (MQ=255)

                                                                                                   cATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGTCATTTAAAGTtg                                    >  2:1912183/1‑140 (MQ=255)

                                                                                                   cATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtg                                    >  2:1165673/1‑140 (MQ=255)

                                                                                                   cATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtg                                    <  1:786716/140‑1 (MQ=255)

                                                                                                    aTCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgc                                   <  1:1473128/140‑1 (MQ=255)

                                                                                                    aTCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGAtgc                                   >  2:1822527/1‑140 (MQ=255)

                                                                                                     tCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                  <  1:170006/140‑1 (MQ=255)

                                                                                                     tCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                  <  2:1043874/140‑1 (MQ=255)

                                                                                                        cacaagTTCAAGAAGGTCTGGAATATAAAATTAAAAATATAGAACTGGCAGGCTTCACTCTTATCGACATTAAAGAAAAACTTTTCGATGGTTCTTAACATGATGTTGAATAAAAAGAAATGGAATTTAAAGTTgcttct                               >  2:1053564/6‑140 (MQ=255)

                                                                                                         aGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCtt                              >  1:1406362/1‑140 (MQ=255)

                                                                                                         aGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCtt                              >  1:2393930/1‑140 (MQ=255)

                                                                                                          gCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCCTTTAAAGTTGCTGCTTc                             >  2:2168345/1‑140 (MQ=255)

                                                                                                             gTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTAt                          >  2:922423/1‑140 (MQ=255)

                                                                                                              ttCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGc                                                                                                                          >  1:544842/1‑43 (MQ=255)

                                                                                                              ttCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCaa                                                                                             <  1:2176025/72‑1 (MQ=255)

                                                                                                              ttCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                         >  2:2336014/1‑140 (MQ=255)

                                                                                                              ttCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                         >  2:661493/1‑140 (MQ=255)

                                                                                                              ttCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                         >  1:1073032/1‑140 (MQ=255)

                                                                                                              ttCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                         >  1:2667204/1‑140 (MQ=255)

                                                                                                              ttCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGAAATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTGGACTCAAAAGAAATTGCATTTAAAGTTGCTGCTTCTATg                         >  1:1669275/1‑140 (MQ=255)

                                                                                                                 aaGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCa                      <  1:661493/140‑1 (MQ=255)

                                                                                                                   gCAGGTCTGGGAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCAtt                    >  2:386677/1‑140 (MQ=255)

                                                                                                                   gCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCAtt                    >  1:211953/1‑140 (MQ=255)

                                                                                                                   gCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCAtt                    >  2:1454613/1‑140 (MQ=255)

                                                                                                                   gCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCAtt                    <  2:2203139/140‑1 (MQ=255)

                                                                                                                        tCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa               >  1:746434/1‑140 (MQ=255)

                                                                                                                        tCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCACGCTTATCGACATTAAAGCAAAAATTTTCGATGGTTCTTACAATGATTTTGAATCAAACTAAATGGCATTTAAATATGCTGCTTCTATGGCATTGaaaa               >  2:161179/1‑140 (MQ=255)

                                                                                                                             aaGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCa          <  1:2215053/140‑1 (MQ=255)

                                                                                                                                 tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATTGCATTGAAAAATGCAGtaa      >  1:1155953/1‑138 (MQ=255)

                                                                                                                                 tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa      >  2:2175842/1‑140 (MQ=255)

                                                                                                                                 tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa      >  2:2097198/1‑140 (MQ=255)

                                                                                                                                 tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa      >  2:929943/1‑140 (MQ=255)

                                                                                                                                 tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa      >  2:1224441/1‑140 (MQ=255)

                                                                                                                                 tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa      >  1:1454492/1‑140 (MQ=255)

                                                                                                                                 tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa      >  2:1964540/1‑140 (MQ=255)

                                                                                                                                 tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa      >  2:1963797/1‑140 (MQ=255)

                                                                                                                                 tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa      >  2:2837046/1‑140 (MQ=255)

                                                                                                                                 tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa      >  2:2721533/1‑140 (MQ=255)

                                                                                                                                 tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa      >  1:2825282/1‑140 (MQ=255)

                                                                                                                                 tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa      >  2:2644457/1‑140 (MQ=255)

                                                                                                                                 tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa      >  2:1892163/1‑140 (MQ=255)

                                                                                                                                   cACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGc    <  1:716673/140‑1 (MQ=255)

                                                                                                                                   cACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGc    <  2:1108251/140‑1 (MQ=255)

                                                                                                                                     cTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCaa  <  2:1743582/140‑1 (MQ=255)

                                                                                                                                     cTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCaa  <  2:893187/140‑1 (MQ=255)

TCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAA  >  NZ_CP034943/132016‑132288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`