Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP034943 3,992,777 G→T L295M (CTG→ATG)  walK ← cell wall metabolism sensor histidine kinase WalK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,992,7770GT100.0% 764.3 / NA 200L295M (CTG→ATG) walKcell wall metabolism sensor histidine kinase WalK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (103/97);  total (103/97)

Only 100 of 212 total aligned reads displayed.

AATTCATCATCCCGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCT  >  NZ_CP034943/3992641‑3992913
                                                                                                                                        |                                                                                                                                        
aaTTCATCATCCCGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCCAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCAtctc                                                                                                                                       <  2:1001321/140‑1 (MQ=255)
      tcatcCCGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAgcgc                                                                                                                                 >  1:552267/1‑140 (MQ=255)
      tcatcCCGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAgcgc                                                                                                                                 >  2:429698/1‑140 (MQ=255)
        atcCCGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATCATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCg                                                                                                                               <  1:2163218/140‑1 (MQ=255)
        atcCCGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCg                                                                                                                               >  1:292373/1‑140 (MQ=255)
        atcCCGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCg                                                                                                                               >  2:2011339/1‑140 (MQ=255)
          cccGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGGAAGCTCGTAATCGGCATTTCTAAGTCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCgtg                                                                                                                             <  1:232800/140‑3 (MQ=255)
          cccGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCggg                                                                                                                             <  1:1701539/140‑1 (MQ=255)
          cccGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCggg                                                                                                                             <  2:1699499/140‑1 (MQ=255)
          cccGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCggg                                                                                                                             <  1:2218149/140‑1 (MQ=255)
          cccGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCggg                                                                                                                             <  2:1394378/140‑1 (MQ=255)
          cccGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCggg                                                                                                                             <  1:2011339/140‑1 (MQ=255)
          cccGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCggg                                                                                                                             <  2:2752680/140‑1 (MQ=255)
              cTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGt                                                                                                                         >  1:1415747/1‑140 (MQ=255)
                        agcagcATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGaat                                                                                                               >  1:1013359/1‑140 (MQ=255)
                             caTAGAATCGTTCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGATGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTg                                                                                                          >  2:583612/1‑140 (MQ=255)
                             caTAGAATCCTGCTGCTCCACTAACTCTTCAAACGTATAATTCTGTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTAAGAATCTCCAGCGCCGGGGTGTTTATGAGAATATTTg                                                                                                          >  2:948547/1‑140 (MQ=255)
                             caTAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTg                                                                                                          >  1:735114/1‑140 (MQ=255)
                             caTAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTg                                                                                                          >  1:1254695/1‑140 (MQ=255)
                               tAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGcc                                                                                                        <  2:457527/140‑1 (MQ=255)
                               tAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGcc                                                                                                        <  1:2329935/140‑1 (MQ=255)
                                       tgctgcTCCACTACATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAACCGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAAGTGCCCCGTGTCg                                                                                                >  2:336197/1‑140 (MQ=255)
                                       tgctgcTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACTTTCAGCATCTCCAGCGCCGGGCTGTTTTAGAGAATAATTGCGGCGTTTct                                                                                                >  2:1432658/1‑139 (MQ=255)
                                       tgctgcTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCg                                                                                                >  2:2833812/1‑140 (MQ=255)
                                       tgctgcTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCg                                                                                                >  2:613180/1‑140 (MQ=255)
                                       tgctgcTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCg                                                                                                >  1:1277872/1‑140 (MQ=255)
                                       tgctgcTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCg                                                                                                >  1:1154791/1‑140 (MQ=255)
                                       tgctgcTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCg                                                                                                >  1:1134873/1‑140 (MQ=255)
                                            ctCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCggg                                                                                                                             <  1:407295/106‑1 (MQ=255)
                                            ctCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCggg                                                                                                                             >  2:407295/1‑106 (MQ=255)
                                            ctCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTtg                                                                                           >  1:227013/1‑140 (MQ=255)
                                            ctCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTtg                                                                                           >  1:2543555/1‑140 (MQ=255)
                                            ctCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTtg                                                                                           >  1:95644/1‑140 (MQ=255)
                                            ctCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTtg                                                                                           >  1:2819328/1‑140 (MQ=255)
                                             tCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTtgt                                                                                          <  1:79024/140‑1 (MQ=255)
                                             tCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTtgt                                                                                          <  2:705404/140‑1 (MQ=255)
                                                 cTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTaa                                                                                                                     <  1:1072125/109‑1 (MQ=255)
                                                 cTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCg                                                                                      >  1:1959438/1‑140 (MQ=255)
                                                 cTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCg                                                                                      <  1:429698/140‑1 (MQ=255)
                                                         tCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGcc                                                                              <  2:2142344/140‑1 (MQ=255)
                                                         tCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGcc                                                                              <  2:1277872/140‑1 (MQ=255)
                                                         tCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGcc                                                                              >  1:1031890/1‑140 (MQ=255)
                                                          cAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCg                                                                             >  2:1202172/1‑140 (MQ=255)
                                                           aaaCGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGt                                                                            <  1:501956/140‑1 (MQ=255)
                                                           aaaCGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGt                                                                            <  2:552267/140‑1 (MQ=255)
                                                           aaaCGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGt                                                                            <  2:1071003/140‑1 (MQ=255)
                                                           aaaCGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGt                                                                            <  1:2585330/140‑1 (MQ=255)
                                                           aaaCGTATAATTCTCTTGAATCCCCAGCAAGATCGTAATCGGCATATCTAAGGCTGTTTCACGTGAAACGTTCAGCCTCTCCAGCGCCGCGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGt                                                                            >  2:1884495/1‑140 (MQ=255)
                                                             aCGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCt                                                                          <  1:532394/140‑1 (MQ=255)
                                                                tataATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTc                                                                       >  1:1436045/1‑140 (MQ=255)
                                                                tataATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTc                                                                       >  1:2672075/1‑140 (MQ=255)
                                                                   aagtctcTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCata                                                                    <  1:2743415/137‑1 (MQ=255)
                                                                   aagtctcTTGAATCCCCAGCAAGCCCCTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCGGTCata                                                                    <  1:2339593/137‑1 (MQ=255)
                                                                   aaTTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCata                                                                    <  1:2209564/140‑1 (MQ=255)
                                                                   aaTTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCata                                                                    <  2:1977658/140‑1 (MQ=255)
                                                                   aaTTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCata                                                                    <  1:312326/140‑1 (MQ=255)
                                                                   aaTTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCata                                                                    <  1:1866727/140‑1 (MQ=255)
                                                                   aaTTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCata                                                                    <  2:180216/140‑1 (MQ=255)
                                                                   aaTTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCata                                                                    <  1:2380871/140‑1 (MQ=255)
                                                                   aaTTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCata                                                                    <  2:1610158/140‑1 (MQ=255)
                                                                   aaTTCTCTTGAATCCCCAGCAAGCTAGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCata                                                                    <  1:1928076/140‑1 (MQ=255)
                                                                     ttCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCAtata                                                                  <  1:852108/140‑1 (MQ=255)
                                                                        tctTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCGGTTTACGAGAAGAATTGCCCCGTTTCGGGTTGTGGCGATAACGCCGTCTGGCATATAGGc                                                               >  2:2536691/1‑140 (MQ=255)
                                                                          ttGAATCCCCAGCCAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCaa                                                             <  2:2847065/140‑1 (MQ=255)
                                                                          ttGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCaa                                                             >  2:2077030/1‑140 (MQ=255)
                                                                          ttGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCaa                                                             >  2:2329249/1‑140 (MQ=255)
                                                                          ttGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCaa                                                             >  2:2713903/1‑140 (MQ=255)
                                                                          ttGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCaa                                                             >  1:696283/1‑140 (MQ=255)
                                                                          ttGAATACCCAGCAAGCAAGTAATAGGCAATACTAAGGCTGTTTCAATTGAAACGTTCAGCATCTCCAGCGCCCGGCTGTTTAAGAGAATAATTGAACCGTTTAGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCaa                                                             >  2:112690/1‑140 (MQ=255)
                                                                                           tCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCttt                                            <  2:1832400/140‑1 (MQ=255)
                                                                                           tCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCttt                                            <  2:1013359/140‑1 (MQ=255)
                                                                                             gTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCt                                          >  2:1354765/1‑140 (MQ=255)
                                                                                              tAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATCACAGAGGCAAGCTTTCTg                                         >  2:2622970/1‑140 (MQ=255)
                                                                                              tAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTg                                         >  2:1253366/1‑140 (MQ=255)
                                                                                                aTCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTgcg                                       <  1:1633608/140‑1 (MQ=255)
                                                                                                aTCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTgcg                                       <  2:236616/140‑1 (MQ=255)
                                                                                                aTCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTgcg                                       <  1:1070834/140‑1 (MQ=255)
                                                                                                  cGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGct                                     <  1:1354765/140‑1 (MQ=255)
                                                                                                  cGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGct                                     <  1:562475/140‑1 (MQ=255)
                                                                                                     cATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTGTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCACGCTTTCTGCGCtctc                                  >  2:264243/1‑140 (MQ=255)
                                                                                                        ttCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCtt                               <  1:668914/140‑1 (MQ=255)
                                                                                                              ggCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCa                         <  2:2543555/140‑1 (MQ=255)
                                                                                                               gCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCAt                        >  2:2469776/1‑140 (MQ=255)
                                                                                                                   tttCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGc                                               <  2:2396105/113‑1 (MQ=255)
                                                                                                                   tttCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGc                                               <  2:1693449/113‑1 (MQ=255)
                                                                                                                   tttCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGcc                    >  1:623578/1‑140 (MQ=255)
                                                                                                                   tttCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGcc                    >  1:2088073/1‑140 (MQ=255)
                                                                                                                   tttCAAGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCAGTGTCGGTTTGTGGCGATAAAGACGTCTGTCATAAAGGCAATAACAGAGCCAAGCTTTCTGCGCTCTCCTTCTGTCATCGcc                    >  1:1780571/1‑140 (MQ=255)
                                                                                                                       aCGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTggg                <  1:548051/140‑1 (MQ=255)
                                                                                                                       aCGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTggg                <  1:2707679/140‑1 (MQ=255)
                                                                                                                       aCGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATACGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTggg                <  1:1610442/140‑1 (MQ=255)
                                                                                                                        cGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGc               <  2:227013/140‑1 (MQ=255)
                                                                                                                             aaCGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGc                                                               >  2:2243468/1‑87 (MQ=255)
                                                                                                                             aaCGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCtt          >  1:1771865/1‑140 (MQ=255)
                                                                                                                             aaCGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCtt          >  1:246956/1‑140 (MQ=255)
                                                                                                                             aaCGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCtt          >  1:2354785/1‑140 (MQ=255)
                                                                                                                                   cagcatCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGct    <  1:1400267/140‑1 (MQ=255)
                                                                                                                                   cagcatCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGct    <  2:88167/140‑1 (MQ=255)
                                                                                                                                     gcatcTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGctct  <  1:2713903/140‑1 (MQ=255)
                                                                                                                                     gcatcTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGctct  <  2:1407647/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
AATTCATCATCCCGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCT  >  NZ_CP034943/3992641‑3992913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: