Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 196,535 C→G 6.2% S99* (TCA→TGA)  cdaA → diadenylate cyclase CdaA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943196,5350CG 6.2% 2039.3 / 15.3 816S99* (TCA→TGA) cdaAdiadenylate cyclase CdaA
Reads supporting (aligned to +/- strand):  ref base C (232/472);  new base G (60/2);  total (342/474)
Fisher's exact test for biased strand distribution p-value = 1.01e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 818 total aligned reads displayed.

CCAGCCAATATTTGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAGCGGGATACCGGAATGGGAGATTATATCGAG  >  NZ_CP034943/196397‑196674
                                                                                                                                          |                                                                                                                                           
   gccaATATTTGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAgg                                                                                                                                         >  1:5355373/1‑140 (MQ=255)
   gccaATATTTGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAgg                                                                                                                                         <  1:4160722/140‑1 (MQ=255)
   gccaATATTTGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGAGTTTTAGCAATTATTATTATTTTTCAGCCTCAGCTGATAAGAGCGCTTGAACAGCTTGGACGCGGCCGCTTTTTTTCAAgg                                                                                                                                         >  1:6781656/1‑140 (MQ=255)
        tatTTGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCgg                                                                                                                                    <  1:606698/140‑1 (MQ=255)
        tatTTGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCgg                                                                                                                                    <  1:2134747/140‑1 (MQ=255)
        tatTTGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCgg                                                                                                                                    <  1:3657880/140‑1 (MQ=255)
        tatTTGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGAGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTAAAGGatccg                                                                                                                                    >  1:6558475/1‑136 (MQ=255)
          tttGGGGCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCa                                                                                                                                  <  2:4359379/140‑1 (MQ=255)
          tttGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCa                                                                                                                                  <  1:6720146/140‑1 (MQ=255)
          tttGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCa                                                                                                                                  <  1:564303/140‑1 (MQ=255)
          tttGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCa                                                                                                                                  <  1:5850505/140‑1 (MQ=255)
          tttGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCa                                                                                                                                  <  1:1446593/140‑1 (MQ=255)
          tttGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCa                                                                                                                                  <  1:997309/140‑1 (MQ=255)
          tttGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCa                                                                                                                                  <  1:2508671/140‑1 (MQ=255)
          tttGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCa                                                                                                                                  >  2:7078470/1‑140 (MQ=255)
          tttGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCa                                                                                                                                  <  1:7654965/140‑1 (MQ=255)
          tttGGGCCTCAGCACGCTTAAATGGCTGATGGACCAAGCTATAACATTGTGATTTTTAGAAATTCTTATTATTTTTCAGCCTGAGCTGAGAAGAGAGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCa                                                                                                                                  <  2:7537311/140‑1 (MQ=255)
             gggCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGc                                                                                                                               >  1:7489967/1‑140 (MQ=255)
              ggCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTGGAACAGCTTGGCCGCGGCCGCTTTTTTTCCACGAGCGGCACGcc                                                                                                                              >  1:5760274/1‑140 (MQ=255)
                  tCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCa                                                                                                                                  >  2:372179/1‑132 (MQ=255)
                    aGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTCTTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTgga                                                                                                                        >  2:3277774/1‑140 (MQ=255)
                    aGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCa                                                                                                                                  <  2:6096810/130‑1 (MQ=255)
                    aGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCCCGCCTGTgga                                                                                                                        >  2:62879/1‑140 (MQ=255)
                    aGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTgga                                                                                                                        >  2:458713/1‑140 (MQ=255)
                    aGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTgga                                                                                                                        >  2:2686709/1‑140 (MQ=255)
                      cACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTggagg                                                                                                                      <  1:5928458/140‑1 (MQ=255)
                         gCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCACTTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAg                                                                                                                   >  1:1316739/1‑140 (MQ=255)
                              tattgatGATGTACCAAGCTATATCATGGGGATTTTTAGCTATTTTTATTATTTTTCATCCTGAGCTGAGAAGAGCTCTTTAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCgcag                                                                                                              <  2:1740038/134‑1 (MQ=255)
                              aaTGGCTGATGGACCAAGCGATAAGATGGGGATTTTTAGGATTTATTATTATTTTTCAGCCTTAGCTGAGAAGAGCTCTTGAACAGCTTGGCGTCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCgcag                                                                                                              <  2:6375222/140‑1 (MQ=255)
                                tGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTGAAGGAGCGGCACGCCGGTGGAGGAAGCgcagca                                                                                                            >  2:2861752/1‑140 (MQ=255)
                                tGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGCGGAAGCgcagcc                                                                                                            >  2:5049097/1‑139 (MQ=255)
                                tGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCgcagca                                                                                                            <  1:3302090/140‑1 (MQ=255)
                                   cTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGaa                                                                                                         <  1:4604333/140‑1 (MQ=255)
                                     gATGGACCAAGCGATAACCTGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGaaaa                                                                                                       <  1:4289480/140‑1 (MQ=255)
                                     gATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAATGAGCGGCACCCCTGGGGAGGACGCGCAGCAGaaaa                                                                                                       >  1:7562919/1‑140 (MQ=255)
                                     gATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGCAGCGCAGCAGaaaa                                                                                                       >  1:2856869/1‑140 (MQ=255)
                                     gATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGaaaa                                                                                                       >  2:5607928/1‑140 (MQ=255)
                                          aCCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTGAAGGAGCGGCACGCCGGTGGGGGAAGCGCAGCAGAAAACAAtt                                                                                                  >  1:316782/1‑140 (MQ=255)
                                             aaGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGATTTTTTTAAAGGAGCGGCACGCCGGTGGAGGAAGCGCAGCAGAaaaaaaattag                                                                                               >  1:3764501/1‑135 (MQ=255)
                                              aGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAgg                                                                                              >  2:109485/1‑140 (MQ=255)
                                              aGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAgg                                                                                              >  2:5687973/1‑140 (MQ=255)
                                               gCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGc                                                                                             <  1:6950154/140‑1 (MQ=255)
                                               gCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGc                                                                                             <  1:919390/140‑1 (MQ=255)
                                                       aTGTGGATTTTTAGCACTTATTATTATTTTTCCGCCTGAGCTGAGAAGAGCGCTTGAACAGCTCGGCCGCGGCCGCTTTTTTTCAAGGAGCGGGACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACaa                                                                                     <  2:2998812/140‑1 (MQ=255)
                                                         ggggATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGaaaa                                                                                                       <  1:1965061/120‑1 (MQ=255)
                                                         ggggATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACaaaa                                                                                   <  1:3867345/140‑1 (MQ=255)
                                                         ggggATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCGGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACaaaa                                                                                   >  2:1944786/1‑140 (MQ=255)
                                                          gggATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAg                                                                                  >  2:7448168/1‑140 (MQ=255)
                                                          gggATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAAAAATTGAGGCCATCACAAAAg                                                                                  >  2:6739241/1‑140 (MQ=255)
                                                          gggATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCGGTGGAGGAAGCGCCCCAGAAAAAAATTGAGGCCAACACAAAAg                                                                                  >  2:6594925/1‑140 (MQ=255)
                                                            gATTTTTATCAATTTTTTTTATTTTTCATCCTGATCTGAGAAGAGCGCTTTTACTGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGca                                                                                <  2:4024656/140‑1 (MQ=255)
                                                            gATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGca                                                                                <  1:2043444/140‑1 (MQ=255)
                                                              tttttAGCAATTATTATTATTTTTCAGCCTGAGCTTAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGcaat                                                                              <  2:919650/140‑1 (MQ=255)
                                                                    tcAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTGGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATta                                                                        <  2:1550818/139‑1 (MQ=255)
                                                                     cAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATtat                                                                       >  2:2492671/1‑140 (MQ=255)
                                                                      aaTTATTAGTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCGTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGCAAACAATTGAGGCCATCACAAAAGCAATCAATtata                                                                      <  1:4859255/140‑1 (MQ=255)
                                                                         tattattattTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAACGCACTCCATTATATgg                                                                   >  2:4293799/1‑140 (MQ=255)
                                                                              ttttttttCAGCCTGAGCTCAGAAGAGCGCGTGAACAGCTTGGCCGCGGACGCATTTTTTCATGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGaaa                                                              <  2:7277770/137‑1 (MQ=255)
                                                                              ttattTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGaaa                                                              <  1:3465341/140‑1 (MQ=255)
                                                                                 tttttGAGGCTGAGCTGAGAAGAGCGGTGGAACATCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCAATCACAAAAGCAATCAATTATATGGCGAAACGc                                                           <  2:2149273/140‑1 (MQ=255)
                                                                                      cAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGtat                                                      <  1:2686709/140‑1 (MQ=255)
                                                                                           tgagctgagAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGgcg                                                 <  1:5049174/140‑1 (MQ=255)
                                                                                           tgagctgagAAGAGCGCTTGAACAGCTAGGCCGCGGCCGCTTTTTTTCGAGGAGCGGCACGCCTGTTGAAGAAGCGCAGCAGAAAACGATTGAGGCCATTACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGgcg                                                 <  2:148222/140‑1 (MQ=255)
                                                                                             agctgagAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGcc                                               <  1:4011963/140‑1 (MQ=255)
                                                                                             agctgagAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGcc                                               <  2:4173236/140‑1 (MQ=255)
                                                                                             agctgagAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGcc                                               <  1:6382694/140‑1 (MQ=255)
                                                                                             agctgagAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGcc                                               <  1:7021630/140‑1 (MQ=255)
                                                                                             agctgagAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGcc                                               <  1:4458516/140‑1 (MQ=255)
                                                                                                  agaagaGCGCTTGAACCGCTGGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCctgct                                          <  2:5895392/140‑1 (MQ=255)
                                                                                                  agaagaGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGgcg                                                 <  1:5423132/133‑1 (MQ=255)
                                                                                                  agaagaGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTAAACGGACCGCAAGCCGGTGGTGGAACCGCACCATCAAACAAAAGACGCCAAAAAAAAAGAAATAAAATAAAATGCGAAAAACCCCATAAGCGCCctgct                                          >  1:6247913/1‑140 (MQ=255)
                                                                                                       agCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGaa                                                                                                         >  2:2277964/1‑72 (MQ=255)
                                                                                                       agCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAAtt                                                                                                  <  1:7270260/79‑1 (MQ=255)
                                                                                                       agCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCGGTGGAGGAAGCGCCGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCGGCTGACCa                                     >  2:834903/1‑140 (MQ=255)
                                                                                                       agCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCCGCACGCCAGTGGAGGAAGCCCCGCAGAAAACAAATGAGGCCATCACAAAACCAAACAAATATATGGCGAAACGCCGTATAGGCCCCCTGCTGACCa                                     >  2:5304876/1‑140 (MQ=255)
                                                                                                         cgcTTGAACAGCTTGGCCGCGGCCGCTTTTTTTGAATGATCGGCACGCCTGGGGCGGAAGCGCAGCAGAAAAAAAATGAGGCCCTCACCAAAGAAAAGAATTAAATTGCGAAACACCCTATAGGCGCCCGGCTGACCaat                                   >  1:1142889/1‑138 (MQ=255)
                                                                                                         cgcTTGAACAGCTTGGCCGCGGCCGCTTTTTTTGAAGGAGCGGCACCCCTGTGGAGGAAGCGCAGCACAAAACAAATTAGGCCAACACAAAAGCAAAAAATTATATGGCGAAACCCCCTATAGGCGCCCGGCTGACCact                                   >  1:6842052/1‑138 (MQ=255)
                                                                                                         cgcTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCCCGCCGGTGGGGGTAGCGGAGCAGAAAAAAATTGAGGCCCTCCCAAAAGCAATAAATTATATGGCGAAACGCCGGATAGGCGCCCGGCTGGcccgt                                   >  1:4731403/1‑137 (MQ=255)
                                                                                                         cgcTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGACGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACCCCGTATAGGCGCCCTGCTGACCAtt                                   >  2:341224/1‑140 (MQ=255)
                                                                                                            ttgcccAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAg                                <  2:279375/135‑1 (MQ=255)
                                                                                                            ttGAACCGCTGGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGACGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAg                                <  1:2292454/140‑1 (MQ=255)
                                                                                                            ttGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAg                                <  1:305072/140‑1 (MQ=255)
                                                                                                            ttGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAg                                <  1:101297/140‑1 (MQ=255)
                                                                                                            ttGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAg                                <  2:1316739/140‑1 (MQ=255)
                                                                                                            ttGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAg                                <  1:7148355/140‑1 (MQ=255)
                                                                                                            ttGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAg                                <  2:2061921/140‑1 (MQ=255)
                                                                                                              tatgaGCTTGGCGGCGGCATCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAATCGCATCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGGGCCCTGCTGACCATTGAGCg                              <  2:7656188/136‑1 (MQ=255)
                                                                                                              gAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAGCg                              <  1:1542043/140‑1 (MQ=255)
                                                                                                              gAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCGGTGGAGGAAGCGCAGCCGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAGCg                              >  2:3864358/1‑140 (MQ=255)
                                                                                                                    ctgggccgcggccgaTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAGCGGGATAc                        <  2:1638796/137‑1 (MQ=255)
                                                                                                                    cTTGGCCGCGGCCGCTTTTTTTCCAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCGCTGCTGACCATTGAGCGGGATAc                        <  1:7401196/140‑1 (MQ=255)
                                                                                                                    cTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAAAAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAGCGGGATAc                        >  2:6456664/1‑140 (MQ=255)
                                                                                                                    cTTCGCCGCGGCGGCTTTGTGTCAATGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGTCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAGCGGGATAc                        <  2:4926730/140‑1 (MQ=255)
                                                                                                                        gccgcggccgcTTTTTTTAAAGGAGCGGCACGCCGGGGGAGGAAGCGCCGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCAGCTGACCATTGAGCGGGATACCGGa                    >  2:3546008/1‑140 (MQ=255)
                                                                                                                         ccgcggccgcTTTTTTTCAAGGAGCGGCACGCCTGTGGCGGAAGCGCAGCAGGAAACAATTGAGGCCATCCCAAAAGCAAGCCAGTATATGGCGAAACGCCGTATAGGCGCCCTGCGGGACAGTGAGCGGGATACCGGaa                   >  2:6940071/1‑140 (MQ=255)
                                                                                                                          cgcggccgcTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAGCGGGATACCGGAAt                  <  1:5517847/140‑1 (MQ=255)
                                                                                                                           gcggccgcTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAGCGGGATACCGGAATg                 <  2:4822869/140‑1 (MQ=255)
                                                                                                                              gccgcTTTTTTTCATGGAGCGGCCCGCCTGTGGCGGAAGCGCAGCAGAAAACAATTGTGGCCATCCCCAAAGCAATCAATTATATTGCGAACCGCCGTATAGGCGCCCGGCTGACCAGTGAGCGGGAGACCggccggggc              >  2:5430942/1‑133 (MQ=255)
                                                                                                                              gccgcTTTTTTTCAAGGAGCGGCACGCCGGTGGCGGAAGCGCCGCACAAAACAATTGAGGCCATCACAAAAGCAATCAAATATATTGCGAAACGCCGTCTAGGCGCCCTGCTGACCAATGAGCGGGCTACCGGAATGGga              >  2:1195996/1‑140 (MQ=255)
                                                                                                                                         tCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAGCGGGATACCGGAATGGGAGATTATATCga   <  1:1676664/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
CCAGCCAATATTTGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAGCGGGATACCGGAATGGGAGATTATATCGAG  >  NZ_CP034943/196397‑196674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: