Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,348,441 C→G 5.5% S147C (TCT→TGT)  EO946_RS17300 → carboxylesterase/lipase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,348,4410CG 5.5% 1276.2 / 2.0 496S147C (TCT→TGT) EO946_RS17300carboxylesterase/lipase family protein
Reads supporting (aligned to +/- strand):  ref base C (196/245);  new base G (38/2);  total (243/253)
Fisher's exact test for biased strand distribution p-value = 8.33e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 499 total aligned reads displayed.

GTGTGGATTCACGGCGGCGCTTTTTATCTTGGAGCGGGCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCGGTGATCCGGATAACGTAACGGTATTTGGAGAATCCGCTGGCGGCAT  >  NZ_CP034943/3348302‑3348579
                                                                                                                                           |                                                                                                                                          
gtgtGGAGTCACGGCGGCGCTTTTTATCTTGGAGCGGGCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTc                                                                                                                                            <  1:7387638/140‑1 (MQ=255)
   tgGATTCACGGCGGCGCTTTTTATCTTGGAGCGGGCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTc                                                                                                                                         >  2:2940417/1‑140 (MQ=255)
     gATTCACGGCGGCGCTTTTTATCTTGGAGCGGGCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTTCATTTGTGTTCGt                                                                                                                                       >  1:1843830/1‑140 (MQ=255)
     gATTCACGGCGGCGCTTTTTATCTTGGAGCGGGCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGt                                                                                                                                       <  1:3792596/140‑1 (MQ=255)
         cACGGCGGCGCTTTTTATCTTGGAGCGGGCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCCGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGGTTGGATTTTTAAAGTTGTCTTGGTTtgt                                                                                                                                   >  1:2338157/1‑139 (MQ=255)
          aCGGCGGCGCTTTTTATCTTGGAGCGGGCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTtgat                                                                                                                                  <  1:4125765/140‑1 (MQ=255)
          aCGGCGGCGCTTTTTATCTTGGAGCGGGCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTtgat                                                                                                                                  <  2:6029149/140‑1 (MQ=255)
          aCGGCGGCGCTTTTTATCTTGGAGCGGGCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTtgat                                                                                                                                  <  2:1255448/140‑1 (MQ=255)
          aCGGCGGCGCTTTTTATCTTGGAGCGGACAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTtgat                                                                                                                                  <  1:7552733/140‑1 (MQ=255)
           cggcggCGCTTTTTATCTTGGAGCGGGCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTtgatg                                                                                                                                 >  2:1066439/1‑140 (MQ=255)
             gcggcgCTTTTTATCTTGGAGCGGGCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAg                                                                                                                               >  2:7239233/1‑140 (MQ=255)
             gcggcgCTTTTTATCTTGGAGCGGGCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAg                                                                                                                               <  1:5585068/140‑1 (MQ=255)
             gcggcgCTTTTTATCTTGGAGCGGGCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAg                                                                                                                               >  2:6242154/1‑140 (MQ=255)
                gcgcTTTTTATCTTGGAGCGGGCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTTTCTTAGTTTGATGAGGCg                                                                                                                            >  2:5277082/1‑140 (MQ=255)
                gcgcTTTTTATCTTGGAGCGGGCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTGTTGGTTTGATGAGGCg                                                                                                                            >  1:1123863/1‑140 (MQ=255)
                gcgcTTTTTATCTTGGAGCGGGCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCg                                                                                                                            <  2:3277693/140‑1 (MQ=255)
                        tATCTTGGAGCGGGCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGa                                                                                                                    <  1:6520894/140‑1 (MQ=255)
                        tATCTTGGAGCGGGCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGa                                                                                                                    <  1:2870498/140‑1 (MQ=255)
                        tATCTTGGAGCGGGCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGa                                                                                                                    <  2:511682/140‑1 (MQ=255)
                        gaTCTTGGAGCGGGCAGTGAGCCATTATTCGCTGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACAGTTGTCTTCGTTTGATGAGGCGTATTCCGa                                                                                                                    <  1:6297436/139‑1 (MQ=255)
                         ttCTTGGAGCGGGCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGAt                                                                                                                   <  2:1294557/139‑1 (MQ=255)
                         aTCTTGGAGCGGGCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCTCAGGGATAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCGGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGAt                                                                                                                   <  2:2429086/140‑1 (MQ=255)
                             tGGAGCGGGCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGtt                                                                                                                                      <  1:6941878/117‑1 (MQ=255)
                                aGCGGGCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCTCAGGGAGAAGTCATTGTCGTTCCACTGAATTATCGACTGGGTCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTg                                                                                                            <  2:2100875/140‑1 (MQ=255)
                                aGCGGGCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTg                                                                                                            <  1:5159703/140‑1 (MQ=255)
                                aGCGGGCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTg                                                                                                            <  1:6014640/140‑1 (MQ=255)
                                    ggCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTtgat                                                                                                                                  <  2:4422850/114‑1 (MQ=255)
                                    ggCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTtgat                                                                                                                                  >  1:4422850/1‑114 (MQ=255)
                                           aGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACCTTTGTCTTCGTTTGATGTGGCGTATTCCGATCACCATGGTGTTTTGGAc                                                                                                 >  1:5061324/1‑140 (MQ=255)
                                               attattCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTGTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAg                                                                                             >  2:6451426/1‑140 (MQ=255)
                                                 tattCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAgcc                                                                                           >  2:2279206/1‑140 (MQ=255)
                                                  attCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAgccg                                                                                          <  2:4632261/140‑1 (MQ=255)
                                                   ttCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAgccgc                                                                                         >  2:3021426/1‑140 (MQ=255)
                                                     cGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTAAATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGccgccg                                                                                       >  2:7470897/1‑140 (MQ=255)
                                                     cGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTGGGAGTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGccgccg                                                                                       >  2:3156042/1‑140 (MQ=255)
                                                      gATGGTTCAAGAATTGCGGCGCTGGTATAATTAATTGTCGTTACACTGAATTATCGTCTGGGGCCGTTTTTATTTTTACATTTGTGTTCGTTTGATGAGGCGTATTCCGATAACCTTGTTCTTTTGGACAAAGCcgccgc                                                                                      <  1:5680705/140‑1 (MQ=255)
                                                       aTGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTTTCTTCGTTTGATGAGGCGTATTCCGATAACCTTTGTCTTTTGGACCAAGCCGCCGCa                                                                                     >  1:2721094/1‑140 (MQ=255)
                                                       aTGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTGGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCa                                                                                     >  1:227405/1‑140 (MQ=255)
                                                        tGGGTCAAGACTTGCGGCGCATGGAGAAGTCATTTTCGTTACCCTGAATTATCGACAGGGGCCGTTTGGAATTTTACAGTTGTCATCGTTTGATTAGGCGTATTCCGATAAACATGGTGTTTTGGAACAAGACTCCCCAc                                                                                    >  2:5850482/1‑140 (MQ=255)
                                                        tGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGTTTTTTACCATTGTCTTGGTTTGATTAGGCGGATTCCGCTAACATTGGTCTTTTTGAACAAGCCGCCGCAc                                                                                    >  1:1436876/1‑140 (MQ=255)
                                                        tGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTAGATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCAc                                                                                    >  1:1271732/1‑140 (MQ=255)
                                                        tGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTAGTTTGATTAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCAc                                                                                    >  1:1027199/1‑140 (MQ=255)
                                                        tGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGCATTTTTACATTTGTCTTCGTTTGTTGAGGCGTATTCCGATAACCTTGTTCTTTTGGACCAAGCCGCCGCAc                                                                                    >  2:611739/1‑140 (MQ=255)
                                                          ggTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTAAATTTGTCTTGGTTTGATGTGGCGTATTACGATAAACATTGTGTTTTGGACCAAGCCGCCGCACTg                                                                                  >  1:3024493/1‑140 (MQ=255)
                                                           gTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGa                                                                                 >  2:2700063/1‑140 (MQ=255)
                                                            tCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGTATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGaa                                                                                <  1:4314500/140‑1 (MQ=255)
                                                              aaGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAAt                                                                              <  2:368503/140‑1 (MQ=255)
                                                              aaGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAAt                                                                              <  2:5598820/140‑1 (MQ=255)
                                                              aaGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAAt                                                                              <  2:773996/140‑1 (MQ=255)
                                                              aaGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAAt                                                                              <  2:4512092/140‑1 (MQ=255)
                                                              aaGACTTGCGGCGCAGGGAGAAGTCATGGTCGTTACACTGAATGATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAAt                                                                              <  2:380430/140‑1 (MQ=255)
                                                                     gcggcgCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTTATGAGGCGTATTCCGATAAACTTGGTCTTTTTGACCAAGCCGCCCCACTGAAAGTGGTGCg                                                                       >  1:6425021/1‑140 (MQ=255)
                                                                     gcggcgCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTTGACCAAGCCGCCGCACTGAAATGGGTGCg                                                                       >  1:7135324/1‑140 (MQ=255)
                                                                     gcggcgCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCg                                                                       <  1:7763320/140‑1 (MQ=255)
                                                                     gcggcgCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTAGTTTGATGAGGCGTATTCCCATAACCTTGGTCTTTTGTACCAACCCGCCGCACTGACATGGGTGCg                                                                       >  1:5431760/1‑140 (MQ=255)
                                                                     gcggcgCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTGTGGATTTTTACAGTTGTCTTGGTTTGATGAGGCGGATTCCGATAACCGTTGTGTTTTGGACCAAGCCGCCGCACGGAACTGGGTGCg                                                                       >  1:3081603/1‑140 (MQ=255)
                                                                      cggcgCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTAAATTTTTGTTCGTTTTTTGTGGCGTATTCCGATAAACATGGTGTTTTTGACCAACCCGCCCCACTGAAAATGGTGCga                                                                      >  1:2056289/1‑140 (MQ=255)
                                                                      cggcgCAGGGAGAAGTCATTGTCGGTAAACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTGTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTATTTTGGACCAAGCCGCCGCACTGAAATGGGTGCga                                                                      <  1:965889/140‑1 (MQ=255)
                                                                           cAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACaa                                                                 >  2:5710781/1‑140 (MQ=255)
                                                                           cAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACaa                                                                 <  2:5047857/140‑1 (MQ=255)
                                                                                atatgTCATTGTCGTTAAACTGAATTATCGACTGGGGCCTTTTGGATTTTTACATTTGTATTCGTTTTATGAGGCTTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATAtct                                                            <  1:917768/136‑1 (MQ=255)
                                                                                agaAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATAtct                                                            <  1:4548944/140‑1 (MQ=255)
                                                                                   aGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGACGCGTATTCCGATAACCTTGGTGTTTTGGACCAATCCGCCCCACTGACAGGGGTGCGAGACAATATCTCAg                                                         >  2:4246873/1‑140 (MQ=255)
                                                                                        ttGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTAAATTTTTGTTCGTTTTTTTATGCGTATTACGATAAACATTGTGTTTTTGACCAAGCCGCCGCACTGAAATGGGTGCGCGACAAAATATAAGCAttt                                                    >  1:585021/1‑140 (MQ=255)
                                                                                         tGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTg                                                   >  2:3638673/1‑140 (MQ=255)
                                                                                         tGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAAAATCTCAGCATTTg                                                   >  2:2576931/1‑140 (MQ=255)
                                                                                            cGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCg                                                <  2:3375313/140‑1 (MQ=255)
                                                                                            cGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCg                                                <  1:3638673/140‑1 (MQ=255)
                                                                                            cGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCg                                                <  2:6536267/140‑1 (MQ=255)
                                                                                              ttACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGTGAGACAATATCTCAGCATTTGGCGGt                                              >  1:6604699/1‑140 (MQ=255)
                                                                                              ttACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCATGGTCTTTTGGACCAAGCCGCCGCCCTGAAATGGGTGCGAGACAAAATCTCAGCCTTTGGCGGt                                              >  1:1705983/1‑140 (MQ=255)
                                                                                              ttACACTGAATTATCGACTGGGGCCGTTTGGATTTTTAAATTTTTATTAGTTTGTTGAGGCGTATTACCATAAACATTGTCTTTTTGACCAAGCCCCCGCCCAGAAAAGGGTGCGAGACAAAATAACAACAGTTGGCGGt                                              >  1:726001/1‑140 (MQ=255)
                                                                                              ttACACTGAATTATCGACTGGGGCCGTGTGGCTTTTTACATTTGTGTTCGTTTGATGAGGCGTATTCCGATAACCATGGTCTTTTGGACCAAGCCGCCGCCCTGAAATGGGTGCGAGACAATATCTAAGCATTTTGCGGt                                              >  1:6760863/1‑140 (MQ=255)
                                                                                               tACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTGTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCACGCCGCCGCACTGAAATTGGTGCGAGACAATATCTCAGCATTTGGCGGTg                                             >  2:6932336/1‑140 (MQ=255)
                                                                                                 cacTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCGGTGAt                                           <  2:155930/140‑1 (MQ=255)
                                                                                                 cacTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCGGTGAt                                           <  1:2557303/140‑1 (MQ=255)
                                                                                                 cacTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCGGTGAt                                           <  1:4865569/140‑1 (MQ=255)
                                                                                                 cacTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCGGTGAt                                           <  2:4728703/140‑1 (MQ=255)
                                                                                                      aaTTATCGACTGGGGCCGTTTTGATTTTTTAATTTGTGTTCGTTTGTTGTTGCGTATTCCGATAACCTTGGTCTTTTTGACCAAGCCCCCGCACTGAAATGGGTGCGAGACAAAATCTCAGCATTTGGCGGTGATACGGa                                      >  1:7628487/1‑140 (MQ=255)
                                                                                                      aaTTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTTGTCTTTTGGACCAAGCCGCCGCCCTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCGGTGATCCGGa                                      >  2:1836416/1‑140 (MQ=255)
                                                                                                      aaTTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCGGTGATCCGGa                                      <  1:2180263/140‑1 (MQ=255)
                                                                                                      aaTTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCGGTGATCCGGa                                      <  1:6210917/140‑1 (MQ=255)
                                                                                                      aaTTATCGAATGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCGGTGATCCGGa                                      <  2:7057711/140‑1 (MQ=255)
                                                                                                           tCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCGGTGATCCGGAtaacg                                 >  2:6712795/1‑140 (MQ=255)
                                                                                                             gccTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACGTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCGGTGTTCCGGAtaacgta                               <  2:1655252/138‑1 (MQ=255)
                                                                                                               cTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATTTGTAATCATTTTGATGTGATCCGGAtaacgtaac                             <  2:859273/140‑1 (MQ=255)
                                                                                                               cTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCGGTGATCCGGAtaacgtaac                             <  1:4657776/140‑1 (MQ=255)
                                                                                                               cTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCGGTGATCCGGAtaacgtaac                             <  1:4001186/140‑1 (MQ=255)
                                                                                                               cTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCGGTGATCCGGAtaacgtaac                             <  1:3532584/140‑1 (MQ=255)
                                                                                                               cTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCGGTGATCCGGAtaacgtaac                             <  1:6080447/140‑1 (MQ=255)
                                                                                                               cTGGGGCCGTTTGGATTTTTACATTTGGCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCGGTGATCCGGAtaacgtaac                             <  1:5256941/140‑1 (MQ=255)
                                                                                                                tGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGGCGTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAAGATCTCAACATTTGGCGGTGCTCCGGAtaacgtaacg                            >  2:6657259/1‑140 (MQ=255)
                                                                                                                       gTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTTGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGGTTTTTGCGTTTATCAGGTTAACGTAACGGTATTTg                     <  2:1633259/140‑1 (MQ=255)
                                                                                                                              tttttACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCGGTGATCCGGATAACGTAACGGTATTTGGAGAATc              <  2:4084015/140‑1 (MQ=255)
                                                                                                                                tttACATTGGTCTTCGTTTGATGAGGCGTATTCCGATAACCGTGGACTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCGGTGATCCGGATAACGTAACGGTATTTGGAGAATCCg            <  1:2796279/140‑1 (MQ=255)
                                                                                                                                  tACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCGGTGATCCGGATAACGTAACGGTATTTGGAGAATCCGCt          >  2:7341522/1‑140 (MQ=255)
                                                                                                                                    cATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCGGTGATCCGGATAACGTAACGGTATTTGGAGAATCCGCTgg        <  1:3300483/140‑1 (MQ=255)
                                                                                                                                        tGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCGGTGATCCGGATAACGTAACGGTATTTGGAGAATCCGCTggcggc    <  1:7442630/140‑1 (MQ=255)
                                                                                                                                          tCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCGGTGATCCGGATAACGTAACGGTATTTGGAGAATCCGCTGGCGGCAt  <  1:1954173/140‑1 (MQ=255)
                                                                                                                                          tCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCGGTGATCCGGATAACGTAACGGTATTTGGAGAATCCGCTGGCGGCAt  <  1:1536364/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
GTGTGGATTCACGGCGGCGCTTTTTATCTTGGAGCGGGCAGTGAGCCATTATTCGATGGGTCAAGACTTGCGGCGCAGGGAGAAGTCATTGTCGTTACACTGAATTATCGACTGGGGCCGTTTGGATTTTTACATTTGTCTTCGTTTGATGAGGCGTATTCCGATAACCTTGGTCTTTTGGACCAAGCCGCCGCACTGAAATGGGTGCGAGACAATATCTCAGCATTTGGCGGTGATCCGGATAACGTAACGGTATTTGGAGAATCCGCTGGCGGCAT  >  NZ_CP034943/3348302‑3348579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: