Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,349,666 T→G 5.4% intergenic (+1/+26) EO946_RS17305 → / ← padC hypothetical protein/phenolic acid decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,349,6660TG 5.4% 1022.5 / 6.9 473intergenic (+1/+26)EO946_RS17305/padChypothetical protein/phenolic acid decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (285/135);  new base G (22/27);  total (309/164)
Fisher's exact test for biased strand distribution p-value = 2.27e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 480 total aligned reads displayed.

TATTTTGTCGGCCGTGTTATGTTTACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCAAATTCAGG  >  NZ_CP034943/3349527‑3349805
                                                                                                                                           |                                                                                                                                           
       tCGGCCGTGTTATGTTTACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCttt                                                                                                                                      >  1:6478919/1‑140 (MQ=255)
                    gTTTACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGACGTGATTCTCTTTTCCGGGTTTTTAt                                                                                                                         <  1:3544043/140‑1 (MQ=255)
                     tttACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATg                                                                                                                        >  1:6096276/1‑140 (MQ=255)
                     tttACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATg                                                                                                                        >  1:7407088/1‑140 (MQ=255)
                     tttACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGAAAAAAGAAAACCCGGGAAAGAGACGGGATTCTCTGTTACGGGTGTTTATg                                                                                                                        >  2:2464463/1‑140 (MQ=255)
                              cacaGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATtata                                                                                                               >  1:138450/1‑140 (MQ=255)
                               acaGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATaa                                                                                                              >  1:5581703/1‑140 (MQ=255)
                                 aGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAAtt                                                                                                            >  2:7041123/1‑140 (MQ=255)
                                     tATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTcc                                                                                                        <  2:5581703/140‑1 (MQ=255)
                                     tATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTcc                                                                                                        <  1:6266282/140‑1 (MQ=255)
                                      aTTACCAGCCTATGGGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAACAAGAAATCCCGGGAAAGAGAGGTGATTCTCTTTGCCGGGGTTTTATGTTTATTAGAATTTGccc                                                                                                       >  1:2331468/1‑140 (MQ=255)
                                       ttACCAGCCTATGCGCTGGACTGTCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCTCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcg                                                                                                      <  1:5832044/140‑1 (MQ=255)
                                       ttACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcg                                                                                                      <  1:5694155/140‑1 (MQ=255)
                                       ttACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcg                                                                                                      <  1:4642498/140‑1 (MQ=255)
                                       ttACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcg                                                                                                      <  2:6114819/140‑1 (MQ=255)
                                       ttACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcg                                                                                                      <  2:2892744/140‑1 (MQ=255)
                                       ttACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcg                                                                                                      <  2:5793931/140‑1 (MQ=255)
                                       ttACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcg                                                                                                      <  2:7615447/140‑1 (MQ=255)
                                       ttACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcg                                                                                                      <  2:5376750/140‑1 (MQ=255)
                                       ttACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGAGTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcg                                                                                                      <  1:5406928/140‑1 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGTTTCCCGCATACTCAGCAAATAGAAAACCCGGGAAAGAGAAGTGAGTCTCTTTTCCGGGTTTTGAGGGTTATTATAATTTTCCCGCGCGta                                                                                                >  1:4455586/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAAGCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTGTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                                >  2:4530340/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGCTTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                                >  2:7170524/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTGCGGGTGTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                                >  1:4910830/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAAt                                                                                                             <  2:7494831/127‑1 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAAt                                                                                                             >  1:7494831/1‑127 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCGCGCGGGta                                                                                                >  2:2365943/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                                >  2:7318523/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                                >  1:1873822/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                                >  2:6750685/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                                >  1:668886/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                                >  2:3788996/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                                >  2:441282/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                                >  2:512047/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTGTTATGTTTATTATAATTTTCCCGCGCGta                                                                                                >  2:4000066/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTGTTATGTTTATTATAATTTTCCCGCGCGta                                                                                                >  2:4114313/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTGCCGGGTTTTTATGTTTATTATAAt                                                                                                             >  1:3653134/1‑127 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACGCAGCAAAAAGACAACCCGGGAAAGAGACGTGATTCTCTGTTGGGGGTTTTTATGTTTGGTATAATTTTCGCGCGCGtc                                                                                                >  2:5202380/1‑139 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAAGTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTGTTCCGGGTGTTTATGTGGATTATAATTTTCCCGCGCGta                                                                                                >  2:5878930/1‑140 (MQ=255)
                                                  tGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCg                                                                                           >  1:3863996/1‑140 (MQ=255)
                                                  tGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCg                                                                                           >  1:5904523/1‑140 (MQ=255)
                                                  tGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTGTTTATGTTTCTTATAATTTTCCCGCGCGTATATCg                                                                                           >  1:1035665/1‑140 (MQ=255)
                                                  tGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTGTTTATGTTTATTGTAATTTTCGCGCGCGTATCTCg                                                                                           >  2:88274/1‑140 (MQ=255)
                                                  tGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGAGTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCg                                                                                           >  2:722163/1‑140 (MQ=255)
                                                       tGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGt                                                                                      <  2:1137542/140‑1 (MQ=255)
                                                         gACTGGCATCCTACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCa                                                                                    <  2:6209632/140‑1 (MQ=255)
                                                         gACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGACGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCa                                                                                    <  2:742946/140‑1 (MQ=255)
                                                         gACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCa                                                                                    >  1:6806550/1‑140 (MQ=255)
                                                         gACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCa                                                                                    >  1:1744/1‑140 (MQ=255)
                                                         gACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCa                                                                                    <  2:3401252/140‑1 (MQ=255)
                                                         gACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCa                                                                                    >  1:1542753/1‑140 (MQ=255)
                                                         gACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATAGTCTGTCa                                                                                    >  2:2689238/1‑140 (MQ=255)
                                                         gACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTGCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCa                                                                                    >  2:4833457/1‑140 (MQ=255)
                                                         gACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGAAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCa                                                                                    <  2:3105544/140‑1 (MQ=255)
                                                           cTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCAtt                                                                                  <  1:7747438/140‑1 (MQ=255)
                                                           cTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCAtt                                                                                  <  2:3640066/140‑1 (MQ=255)
                                                           cTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCAtt                                                                                  <  2:3152299/140‑1 (MQ=255)
                                                           cTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCAtt                                                                                  <  2:2212806/140‑1 (MQ=255)
                                                             ggCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGAGTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTcc                                                                                <  1:905759/140‑1 (MQ=255)
                                                              gCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGTAAACCCGGGAAAGAGAATTGAGTCTCTTTTGCGGGTGTTTATGTTTATTATAATTTTCCCGAGCTTCTATCTTCTGTAATTcat                                                                               >  2:1757287/1‑138 (MQ=255)
                                                              gCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGGAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTcc                                                                                >  1:2575205/1‑139 (MQ=255)
                                                              gCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCt                                                                               >  1:1243847/1‑140 (MQ=255)
                                                              gCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCt                                                                               >  1:4481207/1‑140 (MQ=255)
                                                              gCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCt                                                                               >  1:2930030/1‑140 (MQ=255)
                                                                   atACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATa                                                                          >  2:1871334/1‑140 (MQ=255)
                                                                   atACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATa                                                                          >  1:2954964/1‑140 (MQ=255)
                                                                      cTGTTTTTCGCGCGAATTGAGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAgg                                                                       <  2:832514/140‑1 (MQ=255)
                                                                        gTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAATGAAAACCCGGGAAAGAGAAGTGATTGTCTTTTCCGGGTTTTTATGTTTCTTATACTTTGCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAg                                                                     >  2:1256395/1‑140 (MQ=255)
                                                                        gTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAATGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGGTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAg                                                                     >  1:3210873/1‑140 (MQ=255)
                                                                         tttttCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGTAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCGCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGc                                                                    >  1:1603790/1‑140 (MQ=255)
                                                                         tttttCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGACAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGc                                                                    >  1:600649/1‑140 (MQ=255)
                                                                         tttttCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGc                                                                    >  1:1659286/1‑140 (MQ=255)
                                                                         tttttCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGAGCGTAGATCGTCTGTCATTCCTTCATAAGTAGc                                                                    >  2:4672487/1‑140 (MQ=255)
                                                                         tttttCGCGGGAATTAAGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGt                                                                                                                       <  1:432718/89‑1 (MQ=255)
                                                                            ttCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGACGTGATTGTCTTTTCCGGGGTTTTAGGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCttt                                                                 >  1:6931412/1‑140 (MQ=255)
                                                                                       tATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTActt                                                      <  1:512047/140‑1 (MQ=255)
                                                                                            gacagcccGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGAGTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCg                                                 <  2:1445755/135‑1 (MQ=255)
                                                                                              gTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTAGAAATTACTTCTTAGtt                                               >  1:6371014/1‑140 (MQ=255)
                                                                                                 cccGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTc                                            >  2:4228422/1‑140 (MQ=255)
                                                                                                 cccGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTc                                            >  1:3323395/1‑140 (MQ=255)
                                                                                                 cccGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTc                                            >  1:530732/1‑140 (MQ=255)
                                                                                                   cGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGa                                          <  1:201358/140‑1 (MQ=255)
                                                                                                      aTACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTc                                       <  2:331442/140‑1 (MQ=255)
                                                                                                            atcAAAAAGAAAACCCGGGAAAGATAAGTGAATCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCtgtc                                 >  1:1445755/3‑137 (MQ=255)
                                                                                                            aGCAAAGAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCAt                                 >  2:1003885/1‑140 (MQ=255)
                                                                                                            aGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCAt                                 >  2:4337610/1‑140 (MQ=255)
                                                                                                            aGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCGTTTCCGGGTTTTTATGTGTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATCAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCAt                                 >  2:1519661/1‑140 (MQ=255)
                                                                                                            aGCAAAAAGAAAACCCGGGAAAGAGAAGTGAGTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCAtt                                                                                  <  1:4835262/91‑1 (MQ=255)
                                                                                                              cAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTGTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTGATAAGGAGCTTTGGAAATTACTTGTTGGGTGTCGACTCCCTccgtt                               >  2:4986814/1‑136 (MQ=255)
                                                                                                              cAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCAttt                               >  1:283283/1‑140 (MQ=255)
                                                                                                              cAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCAttt                               >  1:5170748/1‑140 (MQ=255)
                                                                                                                  aaGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATACGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCa                           <  1:7669200/140‑1 (MQ=255)
                                                                                                                  aaGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCa                           <  1:7182475/140‑1 (MQ=255)
                                                                                                                  aaGAAAACCCGGGAAAGAGAAGTGAGTCTATTTTACGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTAGATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCa                           <  2:6371014/140‑1 (MQ=255)
                                                                                                                        aCCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAgg                     >  2:6593014/1‑140 (MQ=255)
                                                                                                                            gggAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAAt                 <  1:1158236/140‑1 (MQ=255)
                                                                                                                                   agaAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCa          >  1:7227674/1‑140 (MQ=255)
                                                                                                                                   agaAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCa          >  1:2086074/1‑140 (MQ=255)
                                                                                                                                    gaAGTGATTCTCTTTTCCGGGTTTTTAGGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCaa         >  1:3108542/1‑140 (MQ=255)
                                                                                                                                        tGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCAAATTc     >  1:3971259/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TATTTTGTCGGCCGTGTTATGTTTACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCAAATTCAGG  >  NZ_CP034943/3349527‑3349805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: