Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,375,849 T→G 5.5% N263H (AAC→CAC)  trxB ← thioredoxin‑disulfide reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,375,8490TG 5.5% 1242.7 / 9.2 480N263H (AAC→CAC) trxBthioredoxin‑disulfide reductase
Reads supporting (aligned to +/- strand):  ref base T (144/289);  new base G (45/2);  total (189/291)
Fisher's exact test for biased strand distribution p-value = 8.42e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 504 total aligned reads displayed.

TGGAGTTCTTCGACATAATGCTGAACGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTTTCTTCACCGGTTACCGTATCGACAAGCGTTA  >  NZ_CP034943/3375710‑3375986
                                                                                                                                           |                                                                                                                                         
tGGAGTTCTTCGACATAATGCTGAACGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCgtt                                                                                                                                           <  2:3713220/140‑1 (MQ=255)
 ggAGTTCTTCGACATAATGCTGAACGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCgttt                                                                                                                                          >  1:1431471/1‑140 (MQ=255)
 ggAGTTCTTCGACATAATGCTGAACGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTGTCGCGACTGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCgttt                                                                                                                                          >  1:899009/1‑140 (MQ=255)
     ttcttcGACATAATGCTGAACGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCgtttgttt                                                                                                                                      <  2:3311321/140‑1 (MQ=255)
     ttcttcGACATAATGCTGAACGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCgtttgttt                                                                                                                                      >  2:2418818/1‑140 (MQ=255)
     ttcttcGACATAATGCTGAACGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTGTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCgtgtgttt                                                                                                                                      >  2:6589448/1‑140 (MQ=255)
       cttcGACATAATGCTGAACGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCg                                                                                                                                    >  1:1277045/1‑140 (MQ=255)
       cttcGACATAATGCTGAACGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCg                                                                                                                                    >  1:5655807/1‑140 (MQ=255)
       cttcGACATAATGCTGAACGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTGTGTTTCg                                                                                                                                    >  1:2738969/1‑140 (MQ=255)
            aCATAATGCTGAACGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTa                                                                                                                               <  2:6888204/140‑1 (MQ=255)
             cATAATGCTGAACGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTGTGTTTGGATTTat                                                                                                                              >  1:2982763/1‑139 (MQ=255)
                 aTGCTGAACGGTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTGTCGATGTTGCctt                                                                                                                          >  2:2138880/1‑140 (MQ=255)
                 aTGCTGAACGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAACATACCTTCAACCTTTGTTTCCATGCGGTCGGGTGTTTCGATGTAGCctt                                                                                                                          >  2:4023952/1‑140 (MQ=255)
                 aTGCTGAACGCTTTGTGCCGCAATACTTCCGTCTCCTGGAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGGTTCCATGCGGTCGTTTGTTTCGATGTAGCctt                                                                                                                          <  2:1674247/140‑1 (MQ=255)
                  tGCTGAACGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATg                                                                                                                                 <  1:6836060/132‑1 (MQ=255)
                       aaCGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCAt                                                                                                                    <  2:7464662/140‑1 (MQ=255)
                       aaCGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTGTGTTTCGATGTAGCCTTCTTCAt                                                                                                                    >  1:4754794/1‑140 (MQ=255)
                           cTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGt                                                                                                                <  2:3394215/140‑1 (MQ=255)
                            tttGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTa                                                                                                               >  1:6519388/1‑140 (MQ=255)
                            tttGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTa                                                                                                               >  2:1020156/1‑140 (MQ=255)
                            tttGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTa                                                                                                               >  2:4257113/1‑140 (MQ=255)
                              tgtgCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTAGATGTAGCCTTCTTCATTAGTAAt                                                                                                             >  2:2116428/1‑140 (MQ=255)
                                     cAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGa                                                                                                      >  1:7575209/1‑140 (MQ=255)
                                     cAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTAGTAATTCCCAGa                                                                                                      >  2:6100877/1‑140 (MQ=255)
                                          cTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTGTGGTTCGCTGTAGCCTTGTTCATTCGTAATGCCCAGATTTTc                                                                                                 >  2:584289/1‑140 (MQ=255)
                                            tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                               <  1:1044220/140‑1 (MQ=255)
                                            tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                               <  2:2499569/140‑1 (MQ=255)
                                            tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                               <  1:7655305/140‑1 (MQ=255)
                                            tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                               <  1:621945/140‑1 (MQ=255)
                                            tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                               <  2:1415831/140‑1 (MQ=255)
                                            tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                               <  1:521543/140‑1 (MQ=255)
                                            tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                               <  1:1557406/140‑1 (MQ=255)
                                            tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                               <  2:801728/140‑1 (MQ=255)
                                            tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                               <  2:7575209/140‑1 (MQ=255)
                                            tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                               <  2:73928/140‑1 (MQ=255)
                                            tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                               <  2:6969419/140‑1 (MQ=255)
                                            tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                               <  1:2589145/140‑1 (MQ=255)
                                            tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                               <  1:3225672/140‑1 (MQ=255)
                                            tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                               <  1:3951790/140‑1 (MQ=255)
                                            tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                               <  2:5578686/140‑1 (MQ=255)
                                            tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                               <  2:5468431/140‑1 (MQ=255)
                                            tCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                               <  2:5071277/140‑1 (MQ=255)
                                             ccGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATTCCCAGATTTTCGaa                                                                                              >  1:7386390/1‑140 (MQ=255)
                                             ccGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGaa                                                                                              <  1:963004/140‑1 (MQ=255)
                                             ccGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGaa                                                                                              >  1:5756132/1‑140 (MQ=255)
                                             ccGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTGTGTTTGGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGaa                                                                                              >  1:3448215/1‑140 (MQ=255)
                                              cGTCTCCTGGAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAAc                                                                                             <  1:69652/140‑1 (MQ=255)
                                                tctcCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACgg                                                                                           >  2:3287270/1‑140 (MQ=255)
                                                  tcCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTGTGTTTCGCTGTAGCCGTCTTCATTCGTAATGCCCAGATTTTCGAACGGtt                                                                                         >  1:3733877/1‑140 (MQ=255)
                                                  tcCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTGTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGtt                                                                                         >  1:1004357/1‑140 (MQ=255)
                                                      gTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGa                                                                                     <  2:891555/140‑1 (MQ=255)
                                                      gTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGa                                                                                     <  2:4624432/140‑1 (MQ=255)
                                                      gTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGa                                                                                     <  1:388192/140‑1 (MQ=255)
                                                      gTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGa                                                                                     <  1:7206905/140‑1 (MQ=255)
                                                      gTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAAGGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGa                                                                                     <  1:4094662/140‑1 (MQ=255)
                                                         gCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACag                                                                                  <  2:2933757/140‑1 (MQ=255)
                                                              tACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                               <  1:3595891/122‑1 (MQ=255)
                                                                  aTTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCAt                                                                         <  2:4910318/140‑1 (MQ=255)
                                                                  aTTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTACTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCAt                                                                         <  2:1277045/140‑1 (MQ=255)
                                                                    ttGGCGCAGTGATTTTTCGCGGATGTCTCCGGCAGCGAAAATCCCTTCAACCTTAGTTTCCATGCGGTCGTTTGTTTCGATATAGCCTTCTTCATTCGTAATGCCCAGGTTTTCAAACGGTTTAGACAGAGGAAGCATGc                                                                       <  1:2100860/140‑1 (MQ=255)
                                                                    ttGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGc                                                                       <  1:4636335/140‑1 (MQ=255)
                                                                    ttGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGc                                                                       <  1:3798805/140‑1 (MQ=255)
                                                                    ttGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGc                                                                       <  2:3799472/140‑1 (MQ=255)
                                                                        cgcAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTGTTTTCGATGTAGCCGTCTTCATTCGTAAAGCCCAGATTTTCGAAAGGTTTATCCGGAGGAAACATGCCGAt                                                                   >  2:1575779/1‑140 (MQ=255)
                                                                           agggACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTa                                                                <  1:177339/137‑1 (MQ=255)
                                                                             tGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCCACCTTTGTTTCCATGCGGTCGTTTGTTTCGTTGGAGCCTTCTTCATTCCTAATGCCCAGCTTTTCGAAAGGTTTAGACAGAGGAAGCATGGCGATGTaaa                                                              >  2:1079797/1‑140 (MQ=255)
                                                                             tGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTGGTTGTATCCGTCTTCATTCGTAATGCCCAGTTTTTCGAACGGTTTAGACCGAGGAAGCATGCCGATGTaaa                                                              >  2:5629080/1‑140 (MQ=255)
                                                                                  tttCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTGGTTTCCATGCGGTCGGGTGTGTGGTTGTGGCCGTCGTAATTAGGAAAGCCCAGAGTTTCGAACGGGTTTGACACGGGGATCATGCCGCTGGAAATGaaa                                                         >  1:2256944/1‑139 (MQ=255)
                                                                                   ttCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATa                                                        <  1:185823/140‑1 (MQ=255)
                                                                                         aaTGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                               >  2:4443432/1‑95 (MQ=255)
                                                                                         aaTGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGa                                                                                               <  1:4443432/95‑1 (MQ=255)
                                                                                         aaTGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGt                                                  <  1:6070267/140‑1 (MQ=255)
                                                                                         aaTGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGt                                                  <  1:4963357/140‑1 (MQ=255)
                                                                                         aaTGTCTCCGGCAGCGAAAATACCGTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTACCTCCTTCGTAATGCCCAGATTTTCGAACGCTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGt                                                  <  1:5914991/140‑1 (MQ=255)
                                                                                           tGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGCTGTAGCCTTCTTCATTAGGAATGCCCAGATTTTGGAAAGGTTTATACAGAGGTAGCATGCCGATGTAAATTAAAAAGCCGTCt                                                >  1:6164949/1‑140 (MQ=255)
                                                                                                    cAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAAt                                       <  2:3073945/140‑1 (MQ=255)
                                                                                                       cGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTGTGTTTCGATGTTGCCTTCTTCATTCGTAATGCCCCGATTTTCGAACGGGTTAGACAGAGGAAGCATGCCGCTGTAAATGAATAAGCCGGCTGTTTTGAAttaa                                    >  1:5105926/1‑138 (MQ=255)
                                                                                                       cGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTGTGTTTAGCTGTATCCTTCTTCATTCGTAAAGCCCAGATTTTCGAACGGTTTAGACACAGGAAGCATGCCGATTTAAATGAATACGCCGTGTGTTTTGAATTCa                                    >  2:203191/1‑140 (MQ=255)
                                                                                                         aaaaTACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGAATTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACt                                  >  2:6266392/1‑140 (MQ=255)
                                                                                                             tcccTTCAACCTTAGTTTCCATGCGGTCGTTTGTTTCGATATAGCCTTCTTCATTCGTAATGCCCAGGTTTTCAAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTttct                              <  2:2289386/138‑1 (MQ=255)
                                                                                                              aCCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTttctt                             <  2:5062276/140‑1 (MQ=255)
                                                                                                               ccTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTttcttc                            <  1:6772707/140‑1 (MQ=255)
                                                                                                                 ttCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTGGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATAAGCCGTCTGTTTTGAATTGACTTTGTTCAc                          >  2:4398849/1‑140 (MQ=255)
                                                                                                                 ttCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTTTCTTCAc                          >  2:1818374/1‑140 (MQ=255)
                                                                                                                         ttGTTTCCATGCGGTCGTTTGTTTCGATGTATCCGTCTTCATTAGTAATTCCCCGATTTTGGAACGGTTTATACAGATGAAGCATTCCGATGTAAATTAAAAAGCCGTCTGTTTTTTATTAACTTTCTTCACCGGTTaac                  >  1:5578721/1‑138 (MQ=255)
                                                                                                                         ttGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTTTCTTCACCGGTTAcc                  <  1:5403910/140‑1 (MQ=255)
                                                                                                                          tGTTTCCATGCGGTCGTTTTTTTCGATGTAGCCTTCTTCATTAGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATTAAAAAGCCGTCTGTTTTGAATTAACTTTCTTCACCGGTTAcag                 >  2:1202036/1‑138 (MQ=255)
                                                                                                                          tGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATTCCCCGAATTTCGAAAGGTTTAGACAGAGGGAGCATGCCGAAGTAAATGAATAAGCCGTGTGTTTTTAATTAACTTTGTTCACCCGtttacg                 >  2:6779342/1‑136 (MQ=255)
                                                                                                                          tGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTAATTCGTAATTCCCAGATTTTCGAAAGGTTTAGACAGAGGAAGCATGCCGATGTAAATTAATAAGCCGTCTTTTTTGTATTCAATTTGTTCACAGGTTaaag                 >  2:5950241/1‑137 (MQ=255)
                                                                                                                                cATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTTTCTTCACCGGTTACCGTATCGa           <  1:247556/140‑1 (MQ=255)
                                                                                                                                 aTGCGTTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTTTCTTCACCGGTTACCGTATCGAc          <  2:5292596/140‑1 (MQ=255)
                                                                                                                                 aTGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTTTCTTCACCGGTTACCGTATCGAc          <  2:6719479/140‑1 (MQ=255)
                                                                                                                                 aTGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTTTCTTCACCGGTTACCGTATCGAc          <  2:7105519/140‑1 (MQ=255)
                                                                                                                                 aTGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTTTCTTCACCGGTTACCGTATCGAc          <  2:2960264/140‑1 (MQ=255)
                                                                                                                                 aTGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTATGTTTTTAATTAAATTTCTTCACCGGTTACCGTATCGAc          <  2:2736448/140‑1 (MQ=255)
                                                                                                                                 aTGCGGTCGTTTGTTTAGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAAAGAATACGCCGTCTGGTTTGAATTAACTTTCTTCACCGGTTAACGTATAGAc          >  2:7212816/1‑140 (MQ=255)
                                                                                                                                 aTGCGGTAGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCTAACTGTTTATACAGATGATTCATGCCTATGTTAATTAATACGCCGTCTGTTTTGAATTTAATTTATTCACCGGTTACCGTATCGAc          <  2:6467841/140‑1 (MQ=255)
                                                                                                                                    cGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTTTCTTCACCGGTTACCGTATCGACAAg       <  2:2537663/140‑1 (MQ=255)
                                                                                                                                      gTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTTTCTTCACCGGTTACCGTATCGACAAGcg     <  1:4285191/140‑1 (MQ=255)
                                                                                                                                        cGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTTTCTTCACCGGTTACCGTATCGACAAGcgtt   <  1:2320318/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
TGGAGTTCTTCGACATAATGCTGAACGCTTTGTGCCGCAATACTTCCGTCTCCTGTAGCCGTTACAATTTGGCGCAGTGACTTTTCGCGAATGTCTCCGGCAGCGAAAATACCTTCAACCTTTGTTTCCATGCGGTCGTTTGTTTCGATGTAGCCTTCTTCATTCGTAATGCCCAGATTTTCGAACGGTTTAGACAGAGGAAGCATGCCGATGTAAATGAATACGCCGTCTGTTTTGAATTCACTTTCTTCACCGGTTACCGTATCGACAAGCGTTA  >  NZ_CP034943/3375710‑3375986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: