BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	240,190	G→T	14.5%	F114L (TTC→TTA)	EO946_RS01360 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	240,190	0	G	T	14.5%	1283.5 / 65.8	655	F114L (TTC→TTA)	EO946_RS01360	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (266/269); new base T (116/3); total (382/273)
Fisher's exact test for biased strand distribution p-value = 5.51e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 690 total aligned reads displayed.

CGGCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAG  >  NZ_CP034943/240051‑240327

cGGCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTGTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTtttgtttg                                                                                                                                           >  2:921585/1‑140 (MQ=255)

 ggCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGa                                                                                                                                          <  1:6772965/140‑1 (MQ=255)

 ggCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGa                                                                                                                                          <  1:921164/140‑1 (MQ=255)

      tggtggAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTAGGATTTTTTTTTGAAGttt                                                                                                                                     >  2:2588059/1‑140 (MQ=255)

       ggtggAGAAGGTGTACGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCTACTATACGGGCGTTTTCTGATTTTTGTTTTAACtttt                                                                                                                                    >  1:5754888/1‑140 (MQ=255)

        gtggAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTGCTAATACGAAGTCGATCATTCCGACTATACGGCCGTTTTATGATTTTTGTTTGAATttttt                                                                                                                                   >  1:6260098/1‑139 (MQ=255)

          ggAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTGTAGTTTTggg                                                                                                                                 >  1:2004081/1‑140 (MQ=255)

           gagaAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGga                                                                                                                                >  1:6480188/1‑140 (MQ=255)

                ggTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGCATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGGTTTTTTTTTGAAGTTTTTGGGGAttt                                                                                                                           >  1:346687/1‑140 (MQ=255)

                ggTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGAttt                                                                                                                           >  1:4907237/1‑140 (MQ=255)

                 gtgtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTg                                                                                                                          <  1:3059358/140‑1 (MQ=255)

                   gtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTGGGAGATTTTaa                                                                                                                        >  2:787737/1‑140 (MQ=255)

                   gtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAATTTTTTGGAGATTTTaa                                                                                                                        >  2:252807/1‑140 (MQ=255)

                   gtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGaa                                                                                                                        <  2:2901672/140‑1 (MQ=255)

                     aaGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGga                                                                                                                                <  2:3886478/130‑1 (MQ=255)

                     aaGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATg                                                                                                                      >  2:460719/1‑140 (MQ=255)

                          agagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCttt                                                                                                                 >  2:1495536/1‑140 (MQ=255)

                          agagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTTAATTTTTTGGAGATTTTTATTGCttt                                                                                                                 >  1:518572/1‑140 (MQ=255)

                           gagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGTTTTTTGTTTGAATTTTTGTGAGATTTTAATTGGtttt                                                                                                                >  1:2218638/1‑140 (MQ=255)

                           gagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCtttt                                                                                                                >  2:1069994/1‑140 (MQ=255)

                            agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATATGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTc                                                                                                               >  1:1217368/1‑140 (MQ=255)

                            agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGAGATTTTAATTGCtttta                                                                                                               >  1:2134327/1‑139 (MQ=255)

                            agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAACTTTTTGGAGATTTGAATTGGTTTTc                                                                                                               >  1:1009165/1‑140 (MQ=255)

                            agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAATTTTTTGGAGATTTTAATTGCtttta                                                                                                               >  1:1421744/1‑139 (MQ=255)

                            agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGGAGTGTGGGGATGTTTGAATGGCTTTTc                                                                                                               >  2:4172386/1‑140 (MQ=255)

                            agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTGGGATTTTTGTTTTTAGTTTTTGGTGCTTTTAATTGCGTTTc                                                                                                               >  1:3958873/1‑140 (MQ=255)

                            agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTAGGAATTTTTTTTTAATTTTTGGGAGATTTGAATGGCTTTTc                                                                                                               >  1:3565468/1‑140 (MQ=255)

                                  ggAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTGTTTAAGTTTTGGGTTTTTTGTATGGGTTTTCCGtttt                                                                                                         >  1:873594/1‑140 (MQ=255)

                                   gAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAATTTTTTGGAGATTTTAATTGCTTTTGCGttttt                                                                                                        >  1:799853/1‑140 (MQ=255)

                                        tctcTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTCCGTTTTTAAAAc                                                                                                   >  2:6195322/1‑140 (MQ=255)

                                         ctctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACa                                                                                                  <  1:985871/140‑1 (MQ=255)

                                         ctctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCAGTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACa                                                                                                  <  2:6058891/140‑1 (MQ=255)

                                              aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAgg                                                                                             <  2:5955267/140‑1 (MQ=255)

                                                  tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                         <  1:2914009/140‑1 (MQ=255)

                                                  tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                         <  2:1226044/140‑1 (MQ=255)

                                                   ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGTTCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGGTTTTGCGTTTTTAAAAAAAGAGGCAAGc                                                                                        >  1:3650768/1‑140 (MQ=255)

                                                   ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTGGGAGATTTTAATTGCTTTTGCGTTTTTAAAAAAAGATGCAAGc                                                                                        >  1:2575295/1‑140 (MQ=255)

                                                   ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATGGGTTTTACGTTTTTTAAAAAAAAGGCAAGc                                                                                        >  1:6012247/1‑140 (MQ=255)

                                                   ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGTATGGCTTTTACGTTTTTAAAAAAAAATGCAAGc                                                                                        >  1:2781735/1‑140 (MQ=255)

                                                   ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                                        >  2:1448597/1‑140 (MQ=255)

                                                   ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTGGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                                        >  1:7687823/1‑140 (MQ=255)

                                                   ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTTAAGTTTTTGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAATAGGCAAGc                                                                                        >  1:2375521/1‑140 (MQ=255)

                                                   ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTACGTTTTTAAAAAAAGAGGCAAGc                                                                                        >  2:6365962/1‑140 (MQ=255)

                                                      attattCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAATTTTTTGGAGATTTTTATTGCTTTTCCGTTTTTTAAACAAGAGGCAAGCCac                                                                                     >  1:584047/1‑139 (MQ=255)

                                                         cttCAGAGATGCTGTTTTTGTATATTTGTTCCAATACGAAGTCGATCCTTCCGACGATACGGGCGTTTTCGGATTTTGGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATa                                                                                  <  2:5842887/139‑1 (MQ=255)

                                                         attCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATa                                                                                  >  2:3263121/1‑140 (MQ=255)

                                                          ttCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGAGTTTTGAATTGCTTTTACGTTTTTAAAAAAAAAGGCAAGCCAGATaa                                                                                 >  1:3771282/1‑140 (MQ=255)

                                                          ttCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATaa                                                                                 >  2:5252738/1‑140 (MQ=255)

                                                               agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTGTAGTTTTGGGAGATTTTAATGGCTTTTACGTTTTTAAAACAAAAGGCAAGCCCGAAAAAcccc                                                                            >  1:7161571/1‑139 (MQ=255)

                                                                    cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTGGGATATTTGAATGTCTTTTACGTTTTTAAAAAAAAAGGCAAGCCAGATAAACCCGacaaa                                                                       >  2:4752169/1‑139 (MQ=255)

                                                                    cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTGAACTTTTGGGATATTTTAATTGCTTTTACCTTTTTAAAAAAAGAGGCAAGCCACAAAAAACCGacaac                                                                       >  1:4132105/1‑140 (MQ=255)

                                                                    cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACAATACGGGCGTTTGCGGATTTTTGTTTGAAATTTTGTTAGAGTTTAAATGATATTCCGTATTTAATACAATATGCAACCCAGAGAAACACGaaaac                                                                       >  2:3012426/1‑140 (MQ=255)

                                                                               tttttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTGGGTGATTTGAATTGCGTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAAACCGACAACAACaaaaaaaa                                                            >  1:1738117/1‑138 (MQ=255)

                                                                                 tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAaataat                                                          >  2:7613325/1‑140 (MQ=255)

                                                                                     ttCTAATACGAAGTCGCTCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                      <  2:5641273/140‑1 (MQ=255)

                                                                                     ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                      <  2:7259443/140‑1 (MQ=255)

                                                                                     ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                      <  2:2251179/140‑1 (MQ=255)

                                                                                     ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                      <  1:113777/140‑1 (MQ=255)

                                                                                     ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                      <  2:3337870/140‑1 (MQ=255)

                                                                                     ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                      <  1:5314870/140‑1 (MQ=255)

                                                                                          aTACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAACGGCTTTTCCCTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGt                                                 <  1:5625191/140‑1 (MQ=255)

                                                                                           tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                <  2:1910829/140‑1 (MQ=255)

                                                                                           tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                <  2:5454766/140‑1 (MQ=255)

                                                                                           acagAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                <  1:6944651/137‑1 (MQ=255)

                                                                                               aaGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAAAAGAGGCAAGCCAGATAAACACGACAACAACAAAAAATAATCTATCAAGTACACt                                            >  2:4263526/1‑140 (MQ=255)

                                                                                                aGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTc                                           <  1:1495536/140‑1 (MQ=255)

                                                                                                 gTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTcc                                          <  1:5434414/140‑1 (MQ=255)

                                                                                                 gTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTcc                                          <  1:6132209/140‑1 (MQ=255)

                                                                                                 gTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTcc                                          <  1:7517341/140‑1 (MQ=255)

                                                                                                 gTCGATCATTACGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTcc                                          <  1:2433273/140‑1 (MQ=255)

                                                                                                     aTCATTCCGACTATACGGGCTTTTTAGGATTTTTGTTTTAAGTTTTGGGAGATTTTAATGGCTTTTACGTTTTTAAAAAAAGATGCAAGCCAGATAAAACAGACAAAAAAAAAAAATAATATATCAATTAAACTAcatga                                      >  2:6726592/1‑139 (MQ=255)

                                                                                                      tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGATGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                     <  1:1325885/140‑1 (MQ=255)

                                                                                                      tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                     <  1:4439139/140‑1 (MQ=255)

                                                                                                      tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                     <  1:1591911/140‑1 (MQ=255)

                                                                                                      tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                     <  2:3689020/140‑1 (MQ=255)

                                                                                                      tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                     <  2:3489812/140‑1 (MQ=255)

                                                                                                      tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                     <  2:7103253/140‑1 (MQ=255)

                                                                                                          tCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATggg                                 <  2:2749674/140‑1 (MQ=255)

                                                                                                          tCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAAAAAACTAGCAACTAAACTCcaagcactgt                                 >  2:6944651/1‑136 (MQ=255)

                                                                                                            cGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTCCGTTTTTAAAAAAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATATAGCAAGTAAACTCCAATCATGGGGt                               >  2:768374/1‑140 (MQ=255)

                                                                                                              aCTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGCGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTACATGCATGGGGTaa                             >  2:6552399/1‑140 (MQ=255)

                                                                                                              aCTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAAGAGGCAACCCACATAAACCCGACCACAACAAAAAAAAATCTATCAAGTAAAATCCATGAATGGGGTaa                             >  2:7030397/1‑140 (MQ=255)

                                                                                                                  taCGGGCGTGTTGGGATTTTTTTTTGAAGTTTTGGGAGATTTTAATGGCGTTTCCGTTTTTAAAACAAGAGGCAACCCAGATAAAACCCCCAACAACAAAAAAAAATAAAGCAAGTAAACACCAAGCAAGGGGGaaataa                         >  1:4191858/1‑136 (MQ=255)

                                                                                                                          ttttCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATGGCTTTTCCGTTTTTAAAACAAGATGCAAGCCAGATAAACCCGACAACAACAAAAAATAAACTATCAAGTAAACTCCATGCATGGGGTAATTCATTAGTTGc                 >  2:4937063/1‑140 (MQ=255)

                                                                                                                            ttCTGATTTTTGTTTGTAGTTTTGGGATATTTGTATGGATTTTCCGTTTTTAAAACAAGAGGCAATCGATATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATTCATGGGGTAATTCATTCGTTGCaa               <  1:4606376/140‑1 (MQ=255)

                                                                                                                            ttCTGATTTTTGTTTGAAGTTTTGTGAGATTTGAATTGATTTTCCGTATTTAAAACAAGAGGCAAGCCAGATAAACCCGACAAAAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa               <  2:4451348/140‑1 (MQ=255)

                                                                                                                            ttCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATGGGTTTTACCTTTTTAAAAAAATAGGCAAGGCATATAAAACCGACAACAACAAAAAATAATATAGCAAGTAAAATACATTCAAGGGGTAATTCAATCGTTGCaa               >  2:7500663/1‑140 (MQ=255)

                                                                                                                            ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa               <  1:7642680/140‑1 (MQ=255)

                                                                                                                            ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa               <  2:6856187/140‑1 (MQ=255)

                                                                                                                            ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa               <  1:3319994/140‑1 (MQ=255)

                                                                                                                            ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa               <  2:2375521/140‑1 (MQ=255)

                                                                                                                            ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa               <  1:1849230/140‑1 (MQ=255)

                                                                                                                            ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTACATGCATGGGGTAATTCATTCGTTGCaa               <  2:5119748/140‑1 (MQ=255)

                                                                                                                            ttCGGATTTTTGTTGGCAGTTTTGGGAGAATTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa               <  1:4726251/140‑1 (MQ=255)

                                                                                                                                gATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAata           <  1:7028827/140‑1 (MQ=255)

                                                                                                                                      tgtttgAAGTTTTGGGAGATTTGAATGGCTTTTCCTTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATaaaaa     <  2:1706051/140‑1 (MQ=255)

                                                                                                                                      tgtttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATaaaaa     <  2:310207/140‑1 (MQ=255)

                                                                                                                                      tgtttgAAGTTTTGGGAGATTTGAATGGCTTTTCAGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATaaaaa     <  2:2058984/140‑1 (MQ=255)

                                                                                                                                       gtttgAAGTTTTGGTAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCTACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAAt    <  1:4995570/140‑1 (MQ=255)

                                                                                                                                         ttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag  <  1:6402328/140‑1 (MQ=255)

CGGCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAG  >  NZ_CP034943/240051‑240327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`