BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,394,417	G→T	8.0%	A161A (GCC→GCA)	ppaX ←	pyrophosphatase PpaX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,394,417	0	G	T	8.0%	1278.7 / 18.9	484	A161A (GCC→GCA)	ppaX	pyrophosphatase PpaX
Reads supporting (aligned to +/- strand): ref base G (172/252); new base T (55/2); total (230/254)
Fisher's exact test for biased strand distribution p-value = 1.87e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 496 total aligned reads displayed.

CTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGA  >  NZ_CP034943/3394280‑3394556

cTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTACGGCGTTTTTACCTGCCAGCACATAGGGGTAATTATATACGACCATAAAGGc                                                                                                                                           >  2:1022584/1‑140 (MQ=255)

cTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGGTGCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATATCCGAACATAATGGc                                                                                                                                           >  2:5393657/1‑140 (MQ=255)

cTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTGTTGCGGCGTTTTTGCCTGCCAGCACAACGTGGTAATTATATGCGACCAAAATggg                                                                                                                                           >  1:6261143/1‑139 (MQ=255)

           aaGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCt                                                                                                                                <  1:5481154/140‑1 (MQ=255)

           aaGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCt                                                                                                                                <  1:4934108/140‑1 (MQ=255)

           aaGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCt                                                                                                                                <  1:1378819/140‑1 (MQ=255)

           aaGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCt                                                                                                                                <  1:5987391/140‑1 (MQ=255)

             gCATATAATGACGCTCATGCTTTGCCAGTGTTTCCGGCACTTTACTGGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCg                                                                                                                              <  1:3398229/140‑1 (MQ=255)

             gCATATAATCAGGCTCCTGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCg                                                                                                                              <  2:2325203/140‑1 (MQ=255)

             gCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCg                                                                                                                              <  2:5745750/140‑1 (MQ=255)

             gCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCg                                                                                                                              <  1:4207544/140‑1 (MQ=255)

             gCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCg                                                                                                                              <  1:2001261/140‑1 (MQ=255)

             gCATATAATCACGCTCCTGCTTTGCCAGTGTTTCCGGCCCTTTCATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCg                                                                                                                              <  1:3831437/140‑1 (MQ=255)

                 atacacAGGATCATGCTTTGCCAGTGTTTCCGGCCCTTTACTCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTc                                                                                                                          <  2:4489017/135‑1 (MQ=255)

                   aaTCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCt                                                                                                                        <  1:6639906/140‑1 (MQ=255)

                      cAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGc                                                                                                                     <  1:2592258/140‑1 (MQ=255)

                       aGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGGGGTAATTTTATCCGACCAAAAATGCTTCCGCCGGCTCGCGTCCTAGCt                                                                                                                    >  2:7319507/1‑140 (MQ=255)

                        ggCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTACGGCGTTTTTCCCAGCCAGCACCTCGTGGTAATTATAACCGACCCAAATGGGATCCCCCGGCTCGCTTCCTAGCTg                                                                                                                   >  2:6027533/1‑140 (MQ=255)

                        ggCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTGTTGTTGCGGCGTTTTTCCCTGCCAGCCCATCGGGGTAATTATATCCGACCAAAATGGCTTCCGCCGGCTCGCTTCCGAGCTg                                                                                                                   >  2:2714016/1‑140 (MQ=255)

                        ggCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTCCGGCGTTTTTGCCTGCCACCCCCTCGTGGTAATTATATGCGACCATAAATGCTTCCGCCCGCGCGCTTGCTAGCTg                                                                                                                   >  1:4001828/1‑140 (MQ=255)

                        ggCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTGTTGCGGCGTTTTTTCCAGCCAGCACAACGGGGTGATTATATCCGACCAAAAATGGGTCCGCCCGCGCGCGTCCGAGcgg                                                                                                                   >  1:675351/1‑138 (MQ=255)

                         gCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGGTTTTCCCTGCCACCACATCGTGGTAATTATCTCCGACCATAAATGCTTACGCCGGCTCGCTTCCAAGCGGt                                                                                                                  >  2:4877054/1‑140 (MQ=255)

                            cATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTGCGGCGTTTTTGCCTGCCCGCCCAGCCTGGTGAGTATATCCGGCCCTAAAGGCTTCCGCCGGCGCGCGGCCTAGCGGtttg                                                                                                               >  1:369013/1‑139 (MQ=255)

                             aTGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTCCGGCGTTTTTACCTGCCAGCACAACGTGGGAATTATATCCGACCAAAATTGCTTCCGCCGGCTCGCTTGCTAGCTGTTTCa                                                                                                              >  2:1582259/1‑140 (MQ=255)

                             aTGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTGCGGCGGTTTTCCCGGCCACCACAGCGTGGTAATTATATCCGCCCCTAATTGGTTCCGCCGGCTCGCTTCCTAGCGGTTTCa                                                                                                              >  1:5568508/1‑140 (MQ=255)

                              tGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGGTTTTCCCTGCCAGCACATCGTGGTAATTATCTACGACCATAAAGGCTTACGCCGGCTCGCTTACTAGCTGTTTCAg                                                                                                             >  2:5506640/1‑140 (MQ=255)

                                   tGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTGCGGGGTTTTTGCGTGCGAGCACAACGTGGTAATTTTTTCCGACCAAAATTGGTTCCGCCGGCGGGCTTCATTGCGGTTTTAGGGCTa                                                                                                        >  1:830695/1‑140 (MQ=255)

                                   tGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTCCGGCGGTTTTCCCTGCCAGCACAACGTGGTATGTTTATCCGACCATAATGTCTTGCGCCGGGTCGCTGCCTAGCTGTTTCAGCGCtt                                                                                                        >  1:5752588/1‑139 (MQ=255)

                                   tGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTGAGGCGTTTTTAGCTGCCCCCACAAAGTGGTAAATATATACGACCAAAAATGCTTACGCCGGCTCGCTTCCGAGGAGTTTAAGAGCTa                                                                                                        >  1:4405285/1‑140 (MQ=255)

                                   tGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTGCGGCGTTTTTGCCGGCCAGCACAACGTGGTAAATATATCACACCAAAAATGCTTACGCCCCCTCGCGTCCGATCGGTTTTAgcgcga                                                                                                        >  1:1013652/1‑138 (MQ=255)

                                   tGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTGTTGCGGCGTTGTTGCCAGCCAGCCCAACGGGGTACTTATATCCGACCAAAAAGGGATACGCCCGCGCGCTTCCGAGCGGTTTGAccgcga                                                                                                        >  1:3454859/1‑138 (MQ=255)

                                    gCCAGTGTTTGCGGCCCTTTAATCGTCGATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTGTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTaa                                                                                                       <  2:4429694/140‑1 (MQ=255)

                                    gCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTaa                                                                                                       <  1:5408096/140‑1 (MQ=255)

                                    gCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTaa                                                                                                       <  2:5844305/140‑1 (MQ=255)

                                    gCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTaa                                                                                                       <  2:1269501/140‑1 (MQ=255)

                                         tgtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTTCGGCGTTTTTCCCAGCCAGCACATCGTGGTAATTATCTCCGACCAAAATGTCTTCCGCCCGCTCCCGTACTAGCTGTTTCAGCGCTAAAAGca                                                                                                  >  2:201769/1‑140 (MQ=255)

                                         tgtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGca                                                                                                  <  1:5215093/140‑1 (MQ=255)

                                         tgtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGca                                                                                                  <  2:3810213/140‑1 (MQ=255)

                                         tgtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGca                                                                                                  <  2:1859267/140‑1 (MQ=255)

                                         tgtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGca                                                                                                  <  1:7319507/140‑1 (MQ=255)

                                         tgtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCGTCCTAGCTGTTTCAGCGCTAAAAGca                                                                                                  <  2:5278540/140‑1 (MQ=255)

                                         tgtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTGTGGTCCGGCGTTTTTCCCTGCCAGCACATCGGGGGAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGca                                                                                                  <  1:1551925/140‑1 (MQ=255)

                                         tgtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTGCGGCGTTTTTCCCTGCCCGCCCATCGTGGTAATTATATCCGACCATAATGGGTTACCCCGGCTCGGGGCCGATGGGTTTAAGCGGTAAAAGca                                                                                                  >  1:5631938/1‑140 (MQ=255)

                                           tgtCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCAGAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGcaca                                                                                                <  2:1967602/138‑1 (MQ=255)

                                               cGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCt                                                                                            <  2:6738364/140‑1 (MQ=255)

                                               cGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCt                                                                                            <  1:103953/140‑1 (MQ=255)

                                               cGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCt                                                                                            <  1:6892567/140‑1 (MQ=255)

                                                     tttAATCGTCCATGCGACTCCGGCCGTTTTTGTTGCGGGGGTTTTCCCTGCCCGCACCACGTGGTAATTATAACCGACCAAAAATGCGTGCGCCCGCGCGCGTGCGAGCGGTTTAAGCGCGAAAAGAACACGCTCgggct                                                                                      >  1:7206194/1‑138 (MQ=255)

                                                     tttAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGAt                                                                                      <  2:6540834/140‑1 (MQ=255)

                                                         aTCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAgg                                                                                  <  2:5631938/140‑1 (MQ=255)

                                                               cATGCGACTCCGGCCGTTTTTGTTGCGGCGTTTTTCCCTGCCAGCCCATCCTGGTAATTATGTCCGACCATAATTGCTTCCGCCCGCTCGCTTGCTAGCAGTTTCACCCCAAAAAGCACCGGCTCGGGGTGACGCttttc                                                                            >  2:6933045/1‑138 (MQ=255)

                                                               cATGCGACTCCGGCCGTTTTTGTTGCGGCGTTTTTCCCGGCCCGCACCTCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGc                                                                            >  2:739348/1‑140 (MQ=255)

                                                               cATGCGACTCCGGCCGTGTTTGTTGCGGCGGTTTTACCGGCCAGCACCTCGTGGTAATTATCGCCGACCATAATGGCTTCCGCCGGCTCGCTTCCAAGCTGGTTGAGCGCTAAAAGCACCCGCTCGGGGTCAGGCGTTGc                                                                            >  2:2979245/1‑140 (MQ=255)

                                                                       tCCGGCCGTTTTTGTTCCGGCGTTTTTACCTGCCCGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCGAAAATCACAGGCTCGGGATCAGGCGTTGCCATCGTca                                                                    >  2:1778125/1‑140 (MQ=255)

                                                                        ccGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTcac                                                                   <  1:6990799/140‑1 (MQ=255)

                                                                        ccGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTcac                                                                   <  1:1778125/140‑1 (MQ=255)

                                                                        ccGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTcac                                                                   <  1:1217315/140‑1 (MQ=255)

                                                                        ccGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTcac                                                                   <  1:4797576/140‑1 (MQ=255)

                                                                        ccGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTAGCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTcac                                                                   <  2:2604278/140‑1 (MQ=255)

                                                                         cGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTcaca                                                                  <  1:2897345/140‑1 (MQ=255)

                                                                         cGGCCGTTTTTGTTCCGGCGTTTTTCACTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTcaca                                                                  <  1:1627592/140‑1 (MQ=255)

                                                                             cGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCAcatcat                                                              <  2:4625633/140‑1 (MQ=255)

                                                                               tttttGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACAtcatca                                                            <  2:4730884/140‑1 (MQ=255)

                                                                               tttttGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACAtcatca                                                            <  2:5191233/140‑1 (MQ=255)

                                                                               tttttGGTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACAtcatca                                                            <  2:4927893/140‑1 (MQ=255)

                                                                                ttttGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCaa                                                           <  2:3888001/140‑1 (MQ=255)

                                                                                                 tCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCa                                          <  2:716415/140‑1 (MQ=255)

                                                                                                     tGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCt                                                                                                         <  1:2972117/73‑1 (MQ=255)

                                                                                                       ccAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAAt                                    >  1:4543502/1‑140 (MQ=255)

                                                                                                                cGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGccctgt                           >  2:2850643/1‑136 (MQ=255)

                                                                                                                cGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGAtt                           >  1:2425388/1‑140 (MQ=255)

                                                                                                                cGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGAtt                           >  1:4483697/1‑140 (MQ=255)

                                                                                                                cGTGGTAATTATCTCCGACCATAAT‑GCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTc                          >  2:2214795/1‑140 (MQ=255)

                                                                                                                 gTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCTTTTCAAAGAATTCGCCGATTc                          >  2:4271425/1‑140 (MQ=255)

                                                                                                                 gTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTc                          >  1:2222583/1‑140 (MQ=255)

                                                                                                                  tGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTcc                         >  2:3980543/1‑140 (MQ=255)

                                                                                                                  tGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTcc                         >  2:115051/1‑140 (MQ=255)

                                                                                                                     tAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGt                      <  2:7207376/140‑1 (MQ=255)

                                                                                                                     tAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCGAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGt                      <  2:2483547/140‑1 (MQ=255)

                                                                                                                     tAATTATCTCCGACCAGAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGt                      <  2:6803181/140‑1 (MQ=255)

                                                                                                                     tAATTATATCCGACCATAATGGCTTCCGCCTGATCGCTTCCTAGCTGTTTCATCGCTAAAAGAACAGTCTGGGTATCAGGCTTTGACATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGt                      <  1:4891989/140‑1 (MQ=255)

                                                                                                                      aaTTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTc                     <  1:5057451/140‑1 (MQ=255)

                                                                                                                      aaTTATCTCCGACCATAATGGCGTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTc                     <  2:436762/140‑1 (MQ=255)

                                                                                                                        ttATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAg                   >  2:3188397/1‑140 (MQ=255)

                                                                                                                        ttATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAg                   >  2:7547641/1‑140 (MQ=255)

                                                                                                                          aTCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCt                 >  2:506729/1‑140 (MQ=255)

                                                                                                                          aTCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCt                 >  2:1313156/1‑140 (MQ=255)

                                                                                                                           tctcCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCtt                <  1:739348/140‑1 (MQ=255)

                                                                                                                             tcCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCCTCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTa              >  1:1880144/1‑140 (MQ=255)

                                                                                                                             tcCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTa              >  2:3392258/1‑140 (MQ=255)

                                                                                                                             tcCGACCAGACTGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTCAAAGCACAGGCTCGGGATCAGGCTGTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTa              <  2:4072117/140‑1 (MQ=255)

                                                                                                                              ccGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAg             >  2:2559019/1‑140 (MQ=255)

                                                                                                                               cGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAgg            <  2:7727889/140‑1 (MQ=255)

                                                                                                                               cGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAgg            <  2:6261143/140‑1 (MQ=255)

                                                                                                                               cGACCATAATGGCTTCCGCAGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCAGCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAgg            >  1:501333/1‑140 (MQ=255)

                                                                                                                                 aCCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGcc          >  1:6438973/1‑140 (MQ=255)

                                                                                                                                  ccATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGccc         <  1:7648488/140‑1 (MQ=255)

                                                                                                                                     tAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTCGGCCCata      >  1:7526156/1‑140 (MQ=255)

                                                                                                                                       aTGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAATGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATAtt    <  2:349748/140‑1 (MQ=255)

                                                                                                                                         ggCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAACAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGa  <  1:2689483/140‑1 (MQ=255)

CTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGA  >  NZ_CP034943/3394280‑3394556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`