Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,446,964 T→A 7.3% M208L (ATG→TTG)  degU ← two‑component system response regulator DegU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,446,9640TA 7.3% 1161.8 / 12.2 430M208L (ATG→TTG) degUtwo‑component system response regulator DegU
Reads supporting (aligned to +/- strand):  ref base T (190/196);  new base A (39/2);  total (232/198)
Fisher's exact test for biased strand distribution p-value = 2.00e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 467 total aligned reads displayed.

TAACTCTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGT  >  NZ_CP034943/3446826‑3447103
                                                                                                                                            |                                                                                                                                           
  aCTCTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCAtttt                                                                                                                                          <  1:5356088/140‑1 (MQ=255)
  aCTCTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCAtttt                                                                                                                                          <  2:2357734/140‑1 (MQ=255)
     ctATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTg                                                                                                                                       <  2:7519388/140‑1 (MQ=255)
     ctATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAAttttttt                                                                                                                                       >  1:2630034/1‑139 (MQ=255)
     ctATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAAttttttt                                                                                                                                       >  2:5935312/1‑139 (MQ=255)
     ctATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAAATTTTTg                                                                                                                                       >  2:1888408/1‑140 (MQ=255)
       aTTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                                     <  1:6687555/140‑1 (MQ=255)
       aTTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                                     <  1:4630628/140‑1 (MQ=255)
       aTTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTATTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                                     <  1:3213384/140‑1 (MQ=255)
        ttGTAAGGGAAACGGCAGCCTAAAAAAAGAACTGCCCAGTAAAAGGCAAGTCTCTTATGATACTATCTCATTTCTACCCAGCCATTTTTAATGCCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGaaa                                                                                                                                    >  1:1816606/1‑140 (MQ=255)
         tGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTAGTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCaaa                                                                                                                                   <  2:623613/140‑1 (MQ=255)
         tGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCACTAAAAGGCAATTCTATTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCaaa                                                                                                                                   <  2:4129622/140‑1 (MQ=255)
             aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCATTTTTTGCAAAatat                                                                                                                               >  2:3116921/1‑140 (MQ=255)
              gggAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTCTTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATAtt                                                                                                                              >  1:2359230/1‑140 (MQ=255)
              gggAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTTCAAAAAAtt                                                                                                                              >  1:332990/1‑140 (MQ=255)
                gAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATCAATTTTTTCAAAAAAATGc                                                                                                                            >  1:3509117/1‑140 (MQ=255)
                 aaaCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCt                                                                                                                           <  2:7129595/140‑1 (MQ=255)
                  aaCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTGAAATTGATTTTTTTGAAAATATTGGTc                                                                                                                          >  1:2884395/1‑140 (MQ=255)
                          ccTAAAAAAAGACCTGCCCAGGAAAAGGCCAGTCTCGTGTTATACTAGCTCATTTCTACCCAGCCATTTTTACTGGCGACCACAACGGCGTGCGGCCGGTCGTGGTCATTGATTTTTTTGGAAATAGTGGTCACATGGtg                                                                                                                  >  1:390917/1‑139 (MQ=255)
                                        tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAACATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                    <  1:7114473/140‑1 (MQ=255)
                                          cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                  <  2:4320906/140‑1 (MQ=255)
                                               tAAAAGGCAAGTCTCGTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTTAATTAAATTTTTTCAAAATATTTGTCACAAGGGTTTTAACGGTTTTGTCACtttt                                                                                             >  1:1657661/1‑137 (MQ=255)
                                                 aaaGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATaa                                                                                           <  1:2213655/140‑1 (MQ=255)
                                                    ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCTGTCGTTTACATTCAATTTTTGAAAAAAATTGCACACAATGGTTTTAACAGTTTTATCACTTATaaaaa                                                                                        >  2:1448765/1‑138 (MQ=255)
                                                    ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACa                                                                                        <  2:58404/140‑1 (MQ=255)
                                                    ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACa                                                                                        <  2:5454934/140‑1 (MQ=255)
                                                    ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCATTTTTTTCAAAATATTTGTCACAAGGTTTTTAACGGTTTTCTCACTGATAAACa                                                                                        >  1:4201758/1‑140 (MQ=255)
                                                    ggCAAGTCTCTTATTATACTACCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACa                                                                                        <  2:26036/140‑1 (MQ=255)
                                                        aGTCTCTTATTATACTATCTCATTTCTACCGAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGTTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                    <  2:4427726/140‑1 (MQ=255)
                                                        aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                    <  1:623162/140‑1 (MQ=255)
                                                        aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                    <  1:4794642/140‑1 (MQ=255)
                                                        aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                    <  1:5779465/140‑1 (MQ=255)
                                                        aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                    <  2:6942041/140‑1 (MQ=255)
                                                         gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCaaa                                                                                                                                   >  1:5185430/1‑92 (MQ=255)
                                                         gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTGACATGGTTTTTCACGGTTTTATCACTGATAAACAATGAt                                                                                   >  2:4741930/1‑140 (MQ=255)
                                                         gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCaaa                                                                                                                                   <  2:5185430/92‑1 (MQ=255)
                                                         gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCGAACTGATAAACAATGAt                                                                                   >  2:1296853/1‑140 (MQ=255)
                                                         gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACCGTTTTATCACTGATAAAAAATGAt                                                                                   >  2:5579119/1‑140 (MQ=255)
                                                         gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTGAAAAAAATTGTCACATGGTTTTTCACCGTTTTCTCAAAGATAAACaaatat                                                                                   >  1:4011997/1‑136 (MQ=255)
                                                         gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCAATTTTTTCAAAAAAATTCACAAAAGGTTTTTAAAGGTTTTTTAAAAGATAAACaaatat                                                                                   >  1:843331/1‑136 (MQ=255)
                                                         gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTAAATTAATTTTTTTCAAAATATTTCTCAAAAGGTTTTTAACGGTTTTATAAATTATaaaaaaatat                                                                                   >  1:2070161/1‑136 (MQ=255)
                                                         gTCTCTTATTATACTATCTCATATCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACAATCATTTTTTGCAAAATATTGCGCACATGGTTTTTCACGGTTTTATCACTGATAAACAATTAt                                                                                   >  2:3280549/1‑140 (MQ=255)
                                                         gTCTCTTATTATACTAGCTCATTTCTACCCAGCCATTTTTAATGGCGACCACACCTGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCTAAATATTGCTCACATGGGGTTTCACGGTTGTCTGACTGATAAACCATGag                                                                                   >  2:5366758/1‑139 (MQ=255)
                                                                   ataCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATAc                                                                         <  1:589698/140‑1 (MQ=255)
                                                                   ataCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATAc                                                                         <  1:3128912/140‑1 (MQ=255)
                                                                    taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTTCTCACATGGTTTTTAACCGTTTTCTCACTGATAAACAATGATTCAACAATAcc                                                                        >  1:3696676/1‑140 (MQ=255)
                                                                    taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAAAAAATCGAAAATGGTTTTTAACATTTTTGTAAATTATAAAAAAATATTAACCaaaaac                                                                        >  1:1138953/1‑136 (MQ=255)
                                                                    taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTAATTTTTTTCAAAATATTTCTCACATGGTTTTTAACGGTTTTCTCACTGATAAAAAATTATTCACCAAAAcc                                                                        >  1:7207542/1‑140 (MQ=255)
                                                                    taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTGAGATGCAATTTTTTGAAAAAATTGCTCACATAGTTTTTATCGGTTTTATCAATGTTAAACAAATATTTACGAATaac                                                                        >  1:6016280/1‑138 (MQ=255)
                                                                    taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTAAATTTTTGAAAAAAATTTCTAAAATTGTTTTTAAAGGTTTTATAACTTATAAAAAAATAATCACCaaaaac                                                                        >  1:103021/1‑136 (MQ=255)
                                                                       tATCTCATTTCTACCCAGCCCTTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACcgcg                                                                     <  2:6087681/140‑1 (MQ=255)
                                                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTTAATTCATTTTTTTCAAAAAATTTCTCACATTGTTTTTAACGGTTTTCTCACTGATAAAAAAAGATTGACCAAAAACGCgg                                                                    >  1:7728377/1‑140 (MQ=255)
                                                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTTCTCACATTGTTTTTCACTGTTTTCTCAATGATAAACAAATAATCACCAATAcccccg                                                                    >  1:7290684/1‑136 (MQ=255)
                                                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCAAATGGTTTTTCACCGTTTTCTCACTGATAAACAATGATTAACCCAAAcccccg                                                                    >  2:912209/1‑136 (MQ=255)
                                                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAATAATGAACACATGGTTTTTCAAGGTTTTCTCACTGATAAACAAAGATTAAACAATAACCCgg                                                                    >  2:7743888/1‑140 (MQ=255)
                                                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCATTTTTTGCAAAAAATTTCTAACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAAAAACGCgg                                                                    >  2:2233115/1‑140 (MQ=255)
                                                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTGCAAAATATTTCTCACATTGTTTTTCACGGTTTTATCACTGATAAAAAATGATTAACCAATAACGCgg                                                                    >  2:524823/1‑140 (MQ=255)
                                                                         tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTTATGACTGATAAACAATGATTAAACAATAACGCGGt                                                                   >  2:6003639/1‑140 (MQ=255)
                                                                         tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATAAATTTTTTCAAAAAAATTCTCAAATGGTTTTTAAACGTTTTATAACTTATAAAAAATGATTCAACAAAAACCCGGt                                                                   >  1:7342010/1‑140 (MQ=255)
                                                                           tcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAc                                                                                                            >  2:1763901/1‑97 (MQ=255)
                                                                           tcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAc                                                                                                            >  2:4502944/1‑97 (MQ=255)
                                                                           tcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAc                                                                                                            <  1:1763901/97‑1 (MQ=255)
                                                                           tcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTAAATTAATTTTTTGCAAAAAATTGCTCACATTGTTTTTCACGGTTTTCTCACTTATAAAAAATTATTAACCAATACCGCGGTTg                                                                 >  2:7407861/1‑140 (MQ=255)
                                                                              tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTcaca                                                                                                                       >  2:7355994/1‑83 (MQ=255)
                                                                              tttCTACCCAGCCATTTATAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAATATTTAAAACATTGTTTTTAACGGTTTTATCACAGATAAACAAATATTAACAAATAACGAGGATGcat                                                              >  2:4968096/1‑138 (MQ=255)
                                                                                  tACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTc                                                          <  1:3619405/140‑1 (MQ=255)
                                                                                   aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAAAATTGCGAACAAGGTTTTTCACGCTTTTGTCACTGATAAACAAAGAGTCACCAAAAAAGCGGTTGGtttttag                                                         >  1:454103/1‑138 (MQ=255)
                                                                                    cccAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTAAATTAATTTTTTGCAAAATATTGCTCAAATGGTTTTTCAAGGTTTTCTCACTTAGAAAAAATGATTCACCAATACCGCGGTTTCTTTTTACg                                                        >  1:1465700/1‑140 (MQ=255)
                                                                                         ccATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATCCATTTTTTACAAATATTTGCGACAGGGGTTTTTACGGTTTTATAACGTTTAAAAAAAATTTAACCAAAAACGACGTTGCGTTTTACCTCTGc                                                   >  1:6988803/1‑140 (MQ=255)
                                                                                          cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                                  <  1:5425236/140‑1 (MQ=255)
                                                                                          cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                                  <  1:1899743/140‑1 (MQ=255)
                                                                                          cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                                  <  1:2510962/140‑1 (MQ=255)
                                                                                          cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTACCGTCTGCa                                                  <  1:5002596/140‑1 (MQ=255)
                                                                                             ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                               <  2:7257826/140‑1 (MQ=255)
                                                                                                      cGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcagc                                      <  1:2793389/140‑1 (MQ=255)
                                                                                                      cGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcagc                                      <  1:6727848/140‑1 (MQ=255)
                                                                                                      cGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcagc                                      <  1:4352535/140‑1 (MQ=255)
                                                                                                      cGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGGTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCCAAACCGCGGTTGCGTTTTCCGGATGCAACCATCAtcagc                                      >  2:3938907/1‑140 (MQ=255)
                                                                                                        aCCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATTATTCTCCAATACCGCGGTTTGTTTTTCCGTCTGCAAGCATCTGCAGCAc                                    >  2:4651527/1‑140 (MQ=255)
                                                                                                          cacaACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAGATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACtt                                  <  2:5042389/140‑1 (MQ=255)
                                                                                                          cacaACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACtt                                  <  2:7451912/140‑1 (MQ=255)
                                                                                                          cacaACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACtt                                  <  2:1148822/140‑1 (MQ=255)
                                                                                                                  cTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTTCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTACGGCTGCAAGCATCTGCAGCACTTCACATTcc                          >  2:4638775/1‑140 (MQ=255)
                                                                                                                    tGCGTCCGGTGGTTTACATTCATTTTTTGCAAAATATTGCTCACATTGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccg                        <  2:6474266/140‑1 (MQ=255)
                                                                                                                    tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccg                        <  1:1332463/140‑1 (MQ=255)
                                                                                                                     gCGTGCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACCTTCccgc                       <  1:5615128/140‑1 (MQ=255)
                                                                                                                      cGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgcc                      <  2:6741424/140‑1 (MQ=255)
                                                                                                                      cGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgcc                      <  2:3608948/140‑1 (MQ=255)
                                                                                                                       gTCCGGTCGTTTACATTCATTTTTTGCAAAATATTTCTAACCAGGTTTTTCAAGGTTTTATCACTGATAAAAAAAGATTGACCAATACCGCGGTTGCTTTTTCCGTCTGCAAACATCTGCAGCACTTCACATTCccgccg                     >  2:207154/1‑140 (MQ=255)
                                                                                                                       gTCCGGTCGTTTACATTAATTTTTTTCAAAAAATTTCTCACATGGTTTTTCACGGTTTTCTGACTGGTAAACAAATATTCAACAATAACGCGGTTGCTTTTTACGTCTGCAACCATCTGCAGCACTTCACATTAccgccg                     >  2:6849070/1‑140 (MQ=255)
                                                                                                                         ccGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAg                   <  1:6482380/140‑1 (MQ=255)
                                                                                                                           ggTCGTTTACATTAATTTTTTGCAAAATATTTCTCACATGGTTTTTCACGGTTTTATCACTGATAAAAAATGATTCACCAAAACCGCGGTTGCTTTTTCCGTCTGCAACCAACTGCAGCACTTAACATTCCCGCCCAGtt                 >  2:4924646/1‑140 (MQ=255)
                                                                                                                           ggTCGTTTACAATCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAAAACCGCGGTTTCTTTTTCCGTCTGCAAGCATCAGCAGCACATCACAATCCCGCCGAGtt                 >  2:5625924/1‑140 (MQ=255)
                                                                                                                            gTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGTTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTa                <  2:1973145/140‑1 (MQ=255)
                                                                                                                              cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTGGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa              <  2:2905649/140‑1 (MQ=255)
                                                                                                                              cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa              <  1:6377695/140‑1 (MQ=255)
                                                                                                                               gTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaaa             <  1:7394016/140‑1 (MQ=255)
                                                                                                                                tttACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat            <  2:2452666/140‑1 (MQ=255)
                                                                                                                                         cattTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATg    <  1:7449169/140‑1 (MQ=255)
                                                                                                                                          attTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATgg   >  1:96978/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                           
TAACTCTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGT  >  NZ_CP034943/3446826‑3447103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: