Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,465,110 C→G 7.1% G711A (GGA→GCA)  EO946_RS17885 ← SpoIID/LytB domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,465,1100CG 7.1% 2392.4 / 20.8 812G711A (GGA→GCA) EO946_RS17885SpoIID/LytB domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (128/600);  new base G (74/2);  total (208/604)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 881 total aligned reads displayed.

CATCGGTATGCTTTTAAGAAAGATCCGCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGCCTATGCCGTGTCCGTATCCTTTACCGCTAATGATAAAGGCGTTTGTATTGCTTGAGACAGA  >  NZ_CP034943/3464971‑3465249
                                                                                                                                           |                                                                                                                                           
cATCGGTATGCTTTTAAGAAAGATCCGCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTc                                                                                                                                             >  2:6090961/1‑140 (MQ=255)
cATCGGTATGCTTTTAAGAAAGATCCGCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTc                                                                                                                                             >  2:3605400/1‑140 (MQ=255)
cATCGGTATGCTTTTAAGAAAGATCCGCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTc                                                                                                                                             <  2:4946845/140‑1 (MQ=255)
cATCGGTATGCTTTTAAGAAAGATCCGCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTc                                                                                                                                             >  1:5244945/1‑140 (MQ=255)
     gTATGCTTTTAAGAAAGATCCGCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGa                                                                                                                                        <  1:3436448/140‑1 (MQ=255)
               aagaaagaTCCGCAAAGTTAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTGCTGGAAAGTaaaaaa                                                                                                                              >  2:7764741/1‑139 (MQ=255)
                   aagaTCCGCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTaa                                                                                                                          <  1:6642697/140‑1 (MQ=255)
                    agaTCCGCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTGCTGGATACTAAAAACTTaat                                                                                                                         >  2:4732924/1‑139 (MQ=255)
                    agaTCCGCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAg                                                                                                                         >  1:7594689/1‑140 (MQ=255)
                     gaTCCGCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGGTTCTAAAAACGTAAGa                                                                                                                        >  2:1445760/1‑140 (MQ=255)
                      aTCCGCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGAt                                                                                                                       <  2:4275561/140‑1 (MQ=255)
                        ccGCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACTCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCg                                                                                                                     <  2:1301024/140‑1 (MQ=255)
                        ccGCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGCCTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCg                                                                                                                     <  2:7211350/140‑1 (MQ=255)
                        ccGCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATGGAGTCGTTCCTGGATAGTAAAAACTTAAGATCg                                                                                                                     <  1:6289974/140‑1 (MQ=255)
                        ccGCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCg                                                                                                                     <  2:1670513/140‑1 (MQ=255)
                        ccGCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCg                                                                                                                     <  1:826352/140‑1 (MQ=255)
                        ccGCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCg                                                                                                                     <  2:287279/140‑1 (MQ=255)
                        ccGCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCg                                                                                                                     <  1:6415699/140‑1 (MQ=255)
                        ccGCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCg                                                                                                                     <  2:750367/140‑1 (MQ=255)
                        ccGCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCg                                                                                                                     <  1:5630993/140‑1 (MQ=255)
                        ccGCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCg                                                                                                                     <  2:6551662/140‑1 (MQ=255)
                        ccGCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCg                                                                                                                     <  1:2793766/140‑1 (MQ=255)
                        ccGCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCg                                                                                                                     <  2:7282562/140‑1 (MQ=255)
                        ccGCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCg                                                                                                                     <  1:3663606/140‑1 (MQ=255)
                        ccGCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCg                                                                                                                     <  2:7054271/140‑1 (MQ=255)
                        ccGCAAAGATAGTCCCTCCAAAGATTGATAATGAGTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCg                                                                                                                     <  2:5441082/140‑1 (MQ=255)
                        ccGCAAAGATAGGCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCg                                                                                                                     <  1:2006748/140‑1 (MQ=255)
                        ccGCAAAGATAGGCCCTCCAAACATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCg                                                                                                                     <  2:5958050/140‑1 (MQ=255)
                          gCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGtt                                                                                                                   <  1:4506477/140‑1 (MQ=255)
                           cAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTTGATAGTAAAAACTTAAGATCGttt                                                                                                                  >  2:5278338/1‑139 (MQ=255)
                                   gTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCGGGATAGTAAAAAATTAATATCGTTTTATATGAt                                                                                                          >  1:3820364/1‑140 (MQ=255)
                                      ccTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTGCTGGATAGTAAAAACTTAATATCGTTGTATAGGGTTGt                                                                                                       >  2:37083/1‑140 (MQ=255)
                                      ccTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCAGGATAGAAAAAACATAAGATCGTTGTATAGGATTGt                                                                                                       >  2:184178/1‑140 (MQ=255)
                                         ccAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCg                                                                                                    >  2:3522540/1‑140 (MQ=255)
                                          cAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCGTCCCTGGACTTCTCTCAATCTTTTAATAGGTTGATAGAGTCGTTGAAGGATATGTAAAACTTAATATCGTTGTATAGGAATGtgagg                                                                                                   >  2:481594/1‑136 (MQ=255)
                                          cAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTGCCGGGATAGGAAAAACTTTAGATAGTTTTATAGGATTGTGCgg                                                                                                   >  1:5136344/1‑140 (MQ=255)
                                             aGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGgcag                                                                                                <  1:2318755/140‑1 (MQ=255)
                                              gATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTGCTGGCTAGTAAAAAATTTATGTCGGTGTATAGGATTGTGCGgcagc                                                                                               >  2:2746602/1‑140 (MQ=255)
                                                  gATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTGCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGct                                                                                           >  1:2151713/1‑140 (MQ=255)
                                                  gATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGct                                                                                           <  1:1469912/140‑1 (MQ=255)
                                                  gATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGct                                                                                           <  1:1768150/140‑1 (MQ=255)
                                                         aTTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCAt                                                                                    <  1:4186351/140‑1 (MQ=255)
                                                         aTTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCAt                                                                                    <  2:109890/140‑1 (MQ=255)
                                                         aTTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCAt                                                                                    <  2:3974534/140‑1 (MQ=255)
                                                         aTTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATAAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCAt                                                                                    <  2:3892626/140‑1 (MQ=255)
                                                            cGTACATGATATAATGAACTAAGGAGGCATACGCCTCCCTTGACTTATATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTTGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTgg                                                                                 <  2:3411719/140‑1 (MQ=255)
                                                              tACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCt                                                                               <  1:6048746/140‑1 (MQ=255)
                                                              tACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCt                                                                               <  1:4347943/140‑1 (MQ=255)
                                                              tACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCt                                                                               <  2:1223658/140‑1 (MQ=255)
                                                                   catATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATa                                                                          <  1:964473/140‑1 (MQ=255)
                                                                   aatatAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATa                                                                          <  2:6410574/139‑1 (MQ=255)
                                                                    atatAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATAc                                                                         <  2:4160458/140‑1 (MQ=255)
                                                                         aTGAACGAAGGAGGGATCCGCCTCCCTGGACTTCTATCAATCTTTTAATACCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCt                                                                    <  2:4560310/140‑1 (MQ=255)
                                                                         aTGAACGAAGGAGGCATCCGCCTCCCTGGACTTCTATCAATCTTTTAATAGCGTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCt                                                                    <  1:5968876/140‑1 (MQ=255)
                                                                         aTGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCt                                                                    <  1:7340998/140‑1 (MQ=255)
                                                                         aTGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCt                                                                    <  1:1011478/140‑1 (MQ=255)
                                                                         aTGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCt                                                                    <  1:256829/140‑1 (MQ=255)
                                                                         aTGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCt                                                                    <  2:7357737/140‑1 (MQ=255)
                                                                         aTGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCt                                                                    <  1:4673686/140‑1 (MQ=255)
                                                                         aTGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCt                                                                    <  1:5784787/140‑1 (MQ=255)
                                                                         aTGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCt                                                                    <  2:3056201/140‑1 (MQ=255)
                                                                         aTGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCt                                                                    <  2:2598162/140‑1 (MQ=255)
                                                                         aTGAACGAAGGAGGCATACGCCTACCTGGACTTCTATCAATCTTTTAATAGCTTGATAGACTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCt                                                                    <  2:1529001/140‑1 (MQ=255)
                                                                           gAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATa                                                                          <  1:2997401/132‑1 (MQ=255)
                                                                            aaCGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGgcag                                                                                                <  1:7236891/109‑1 (MQ=255)
                                                                            aaCGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTGCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTTTACGGCAGCAGCTCTGGCATTGGCTCCATACTTGCTCAt                                                                 >  2:2125968/1‑140 (MQ=255)
                                                                             aCGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTGCTGGGTAGTAAAAAATTAAGATAGTTTTATAGGATTTTGCGGCAGCAGCTCTGGGATTGGCTCCATACTTGGTCAtt                                                                >  1:6979925/1‑139 (MQ=255)
                                                                             aCGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATAGTTGTATAGGATTGTCCGGCCGCAGCTCTGGGATTGGCTCCATACTGGCTCATg                                                                >  2:6693841/1‑140 (MQ=255)
                                                                               gAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGcc                                                              <  1:6767481/140‑1 (MQ=255)
                                                                               gAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACGTAAGATCGTTGTATAGGATTTTGCGGCAGCAGCTCTGGCATTGGCTCCATACTTGCTCATGcc                                                              >  2:6189080/1‑140 (MQ=255)
                                                                                aaGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTGGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGCCt                                                             <  2:2312369/140‑1 (MQ=255)
                                                                                   gaggCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGGTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGCCTATg                                                          <  1:534922/140‑1 (MQ=255)
                                                                                     ggCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGCCTATGcc                                                        <  1:5410248/140‑1 (MQ=255)
                                                                                     ggCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGCCTATGcc                                                        <  2:244737/140‑1 (MQ=255)
                                                                                      gCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGCCTATGCCg                                                       <  2:907909/140‑1 (MQ=255)
                                                                                         tACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGCCTATGCCgtgt                                                    <  1:395904/140‑1 (MQ=255)
                                                                                           cGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGCCTATGCCGTGTcc                                                  <  1:3522540/140‑1 (MQ=255)
                                                                                             ccTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGCCTATGCCGTGTCCGt                                                <  2:4064441/140‑1 (MQ=255)
                                                                                              cTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGCCTATGCCGTGTCCGTa                                               <  1:3222112/140‑1 (MQ=255)
                                                                                              cTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGCCTATGCCGTGTCCGTa                                               <  2:5820848/140‑1 (MQ=255)
                                                                                              cTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAAATTAAGATAGTTGTATAGGAATTTACGGCACCAGCTCTGGCATTTGCTCCATACTGGCTCAAGCCGATGCCGTGTCCGTa                                               >  2:4641361/1‑140 (MQ=255)
                                                                                                   tGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGCCTATTCCGTGTCCGTATCCtt                                          <  1:1580645/140‑1 (MQ=255)
                                                                                                   tGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGCCTATGCCGTGTCCGTATCCtt                                          <  2:6943628/140‑1 (MQ=255)
                                                                                                     gACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGCCTATGCCGTGTCCGTATCCTTTa                                        <  2:5587053/140‑1 (MQ=255)
                                                                                                       cTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTGGCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTTTGCGGCAGCAGCTCTGGCATTTGCTCCATACTGGCTCATGCCTTTTACGTGTCCGTATCCTTTAcc                                      >  2:7655775/1‑140 (MQ=255)
                                                                                                        ttCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGCCTATGCCGTGTCCGTATCCTTTACCg                                     <  1:6887977/140‑1 (MQ=255)
                                                                                                          cTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGCCTATGCCGTGTCCGTATCCTTTACCGCt                                   <  1:5480962/140‑1 (MQ=255)
                                                                                                           tATCAATCTTTTAATAGCTTGATAGAGTCGTTGCGGGATAGTAAAAACTTAATATCGTTGTATAGGATTTTGCGGCAGCAGCACTGGCATTGGCTCCAAAATGGCTCATGCCTATGCCCTGTGCGTATCCTTTAACGCTa                                  >  1:1303057/1‑140 (MQ=255)
                                                                                                           tATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGGTAGTAAAAAATTAAGAAGCTTGTATAGGAGTGTGCGGCAGCAGCTCTGGCAGTGGCGCCAAACTGGCGGATGCCGATTCCGTGTCCGGATAACGTACCGCTa                                  >  2:4422008/1‑140 (MQ=255)
                                                                                                                       agtAGCTTGATAGAGTCGTTGCGGGATAGTAAAAACTTAATATCGTTGTATATGATTGTGCGGCAGCACCTCTGGCATTGGCTCCATACTGGCTCATGCCTATGCCCTGTGCGTGTCCTTTAACGCTAAAGATAAATGCg                      >  1:4832280/3‑140 (MQ=255)
                                                                                                                             ttGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGCCTATGCCGTGTCCGTATCCTTTACCGCTAATGATAAAGGCGTTTGTa                <  1:653620/140‑1 (MQ=255)
                                                                                                                             ttGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGCCTATGCCGTGTCCGTATCCTTTACCGCTAATGATAAAGGCGTTTGTa                <  1:7723669/140‑1 (MQ=255)
                                                                                                                             ttGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGCCTATGCCGTGTCCGTATCCTTTACCGCTAATGATAAAGGCGTTTGTa                <  1:3345075/140‑1 (MQ=255)
                                                                                                                               gATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATAGTTTTATAGGATTTTCCGGCAGCAGCTCTTGCCATTGCTCCATACATGCTCATTCCTATGCCGTGTCCGTAACCTTTAACCCTAAATATAAAGGGGTTTGTAtt              >  2:5043873/1‑140 (MQ=255)
                                                                                                                                         ttCCTGGATAGTAAAAACTTAAGATCGGTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGCCTATGCCGTGTCCGTATCCTTTACCGCTAATGATAAAGGCGTTTGTATTGCTTGagaca    <  2:330251/140‑1 (MQ=255)
                                                                                                                                           ccTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGCCTATGCCGTGTCCGTATCCTTTACCGCTAATGATAAAGGCGTTTGTATTGCTTGagacaga  <  1:1129448/140‑1 (MQ=255)
                                                                                                                                           ccTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGCCTATGCCGTGTCCGTATCCTTTACCGCTAATGATAAAGGCGTTTGTATTGCTTGagacaga  <  1:4968789/140‑1 (MQ=255)
                                                                                                                                           ccTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGCCTATGCCGTGTCCGTATCCTTTACCGCTAATGATAAAGGCGTTTGTATTGCTTGagacaga  <  2:5196772/140‑1 (MQ=255)
                                                                                                                                           ccTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGCCTATGCCGTGTCCGTATCCTTTACCGCTAATGATAAAGGCGTTTGTATTGCTTGagacaga  <  2:3820364/140‑1 (MQ=255)
                                                                                                                                           ccTGGAGAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGCCTATGCCGTGTCCGTATCCTTTACCGCTAATGATAAAGGCGTTTGTATTGCTTGagacaga  <  1:6182785/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CATCGGTATGCTTTTAAGAAAGATCCGCAAAGATAGTCCCTCCAAAGATTGATAATGATTCGTACATCATATAATGAACGAAGGAGGCATACGCCTCCCTGGACTTCTATCAATCTTTTAATAGCTTGATAGAGTCGTTCCTGGATAGTAAAAACTTAAGATCGTTGTATAGGATTGTCCGGCAGCAGCTCTGGCATTGGCTCCATACTGGCTCATGCCTATGCCGTGTCCGTATCCTTTACCGCTAATGATAAAGGCGTTTGTATTGCTTGAGACAGA  >  NZ_CP034943/3464971‑3465249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: