Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,496,440 G→T 6.1% Q330K (CAA→AAA)  EO946_RS17995 ← glucosaminidase domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,496,4400GT 6.1% 1451.8 / 22.9 503Q330K (CAA→AAA) EO946_RS17995glucosaminidase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (103/356);  new base T (42/2);  total (145/358)
Fisher's exact test for biased strand distribution p-value = 9.35e-23
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 503 total aligned reads displayed.

AGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCACTGTATTCAGCCCTTTGAAATA  >  NZ_CP034943/3496302‑3496578
                                                                                                                                          |                                                                                                                                          
agGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGc                                                                                                                                           <  2:853373/140‑1 (MQ=255)
    tGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGGCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGGTGGGTGACGTGTAATGCCATTTTTTGTTTTTAAAGAAATTGGCTATGTTTTGCGgct                                                                                                                                       >  1:5554162/1‑140 (MQ=255)
          tttgtttgCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGtt                                                                                                                                 <  1:6753908/140‑1 (MQ=255)
          ttggtgtgcttgctGCTCGCCTAAAATCGGCGCCGTCGTTATTGGAGAGGCTGTAGCCATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGtt                                                                                                                                 <  2:6709782/134‑1 (MQ=255)
                 gcttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACTTGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTaa                                                                                                                          <  1:2282003/140‑1 (MQ=255)
                 gcttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTaa                                                                                                                          <  2:5585797/140‑1 (MQ=255)
                 gcttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTaa                                                                                                                          <  2:205330/140‑1 (MQ=255)
                 gcttgctGCTCGCATAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTaa                                                                                                                          <  1:4566336/140‑1 (MQ=255)
                   ttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGt                                                                                                                                  >  2:5804917/1‑130 (MQ=255)
                   ttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcc                                                                                                                        <  2:2552494/140‑1 (MQ=255)
                   ttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcc                                                                                                                        <  2:6002623/140‑1 (MQ=255)
                     gctgctCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTTTAGACATGGTCGATTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcccc                                                                                                                      <  2:4231479/140‑1 (MQ=255)
                       tgctCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCtt                                                                                                                    <  2:6205643/140‑1 (MQ=255)
                        gctCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTGTTTTTGATGCAGGCTGACATGTAATGCCATTTTTTAATTTTAAAAAAATTTTGGATATTTTGAAACAGCTGTTTAGTTAAcaccata                                                                                                                   >  2:3201001/1‑137 (MQ=255)
                           cGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCata                                                                                                                <  2:7202471/140‑1 (MQ=255)
                           cGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCata                                                                                                                <  2:7326369/140‑1 (MQ=255)
                             ccTAAAATCTGCGGCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                                                                              <  1:5653030/140‑1 (MQ=255)
                             ccTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                                                                              <  1:2862924/140‑1 (MQ=255)
                             ccTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                                                                              <  1:1991805/140‑1 (MQ=255)
                             acTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                                                                              <  2:4350513/139‑1 (MQ=255)
                              cTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTCGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTc                                                                                                             <  1:3475731/140‑1 (MQ=255)
                              cTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTc                                                                                                             <  1:7337553/140‑1 (MQ=255)
                              cTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTc                                                                                                             <  1:2009622/140‑1 (MQ=255)
                               tAATATGTGCGGCTTCGTTATTGTATAGGGGGTTGTCATTGTCTTTTTTTTGATGCTGGATTACGTGTAATGAAATATTTTCTTGTTAATTAAAGTGTATATCTTTTGCAGCTGCTGTTTCTTTAACCCATTCATATTCa                                                                                                            <  2:5399565/140‑1 (MQ=255)
                               tAAAATCTGCGCCGTCGTTATTGGCTAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCa                                                                                                            <  2:6454426/140‑1 (MQ=255)
                               tAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCa                                                                                                            <  1:3384686/140‑1 (MQ=255)
                               tAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCa                                                                                                            <  2:3100776/140‑1 (MQ=255)
                               tAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCa                                                                                                            <  1:1050633/140‑1 (MQ=255)
                               tAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCa                                                                                                            <  2:7065558/140‑1 (MQ=255)
                               tAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCa                                                                                                            <  1:5248913/140‑1 (MQ=255)
                               tAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCa                                                                                                            <  1:1674735/140‑1 (MQ=255)
                               tAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGGTTCGTTAACCCCTTCATATTCa                                                                                                            <  1:6221217/140‑1 (MQ=255)
                               tAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGACATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCa                                                                                                            <  1:5086198/140‑1 (MQ=255)
                                aacaTCTGCGCCCTCCTTATGGGATAGGCTGTAGACATGGTCGTTTGTTTGCTCCTGGATGACGTGTAACGCCATTTTTTCTTTTTAAAGAAAGGGTCTATCTTTTGCAGCTGCAGGATCGTTAACCCCTACATATTCAc                                                                                                           <  2:82513/137‑1 (MQ=255)
                                aaaaTCTGCGCCGTCGTTATTGTATAGGCTGTAGAGATGGTCGTTTTTTTGATGCCGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                                                                                           <  2:1523852/140‑1 (MQ=255)
                                aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTTGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                                                                                           <  1:2759946/140‑1 (MQ=255)
                                aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                                                                                           <  2:468878/140‑1 (MQ=255)
                                aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                                                                                           <  1:4981397/140‑1 (MQ=255)
                                aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                                                                                           <  1:1447000/140‑1 (MQ=255)
                                aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                                                                                           <  1:2456606/140‑1 (MQ=255)
                                aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTATATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                                                                                           <  2:1904062/140‑1 (MQ=255)
                                aaaaTCTGCGCCGTCGTTATTGGAGAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTCAAGAAAGTGTCTATCTTTTGCTGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                                                                                           <  2:6579515/140‑1 (MQ=255)
                                      tGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATTCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAATAAATTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAACCCTTCATAATCAAAGtata                                                                                                     >  2:3706928/1‑140 (MQ=255)
                                        cgcCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATatg                                                                                                   <  1:5785995/140‑1 (MQ=255)
                                        cgcCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATatg                                                                                                   <  1:5919746/140‑1 (MQ=255)
                                        cgcCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATatg                                                                                                   <  1:3039492/140‑1 (MQ=255)
                                        cgcCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATatg                                                                                                   <  2:2718132/140‑1 (MQ=255)
                                          ccGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCCCTGTATatgat                                                                                                 <  2:1702053/140‑1 (MQ=255)
                                          ccGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATatgat                                                                                                 <  2:6907937/140‑1 (MQ=255)
                                          ccGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATatgat                                                                                                 <  2:238292/140‑1 (MQ=255)
                                                      gATAGGCTGGAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCa                                                                                     <  1:2609969/140‑1 (MQ=255)
                                                        tAGGCTGTAGACATGGTCGTTTTTTTTATGCTGGATTAAGTGTAATGCCAATTTTTGTTTTTAAAAAAATTTTGGAAGATTTTCAGCTGCTGTTTCGTTAAACCCATAAAATTAAATTTATATGATGTGGATTTTTCAAc                                                                                   >  2:7490888/1‑140 (MQ=255)
                                                        tAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAAc                                                                                   <  1:3949836/140‑1 (MQ=255)
                                                        tAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAAc                                                                                   <  1:1900433/140‑1 (MQ=255)
                                                         aGGCTGTAGACATGGTCGTTTTTTTGATTCTGGGTGTCGTGGAATGCCATTTTTTATTTTTAAAGAACGTGTCTTTCTTTTGCAGCTGCTGTTTGGTTAAACCCATAATATTGACAGTATATGATGTGGTTTTTTCaaac                                                                                  >  2:2178841/1‑138 (MQ=255)
                                                             tGTAGACATGGTCGTTTTTTTGATGGGGGATTACGTGTAATTCCATTTTTTGTTTTTAAAGAAAGTGGGTATCTTTTGCAGCTGCTGTTTCGTTAACCCCGTCATATTCACAGTAAATTATTTTGTTTTGTAAACCttgt                                                                              >  2:1818862/1‑137 (MQ=255)
                                                                aGACATGGTCGTTTTTTTGTTGCTGGATGACGTGTAATGCCCTTTTTTCTTTTTAAAGAAACTGTCGATCTTTTGGAGCTGCTGTTTGGTTAAACCCATAATATTAACTGTATATGATGTTGTTTTTTAAAACTGTTGAt                                                                           >  2:1983559/1‑140 (MQ=255)
                                                                aGACATGGTCGTTTTTTTGATGCTGGATGTCGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTTCATCTGCTGTTTCGTTAACCCCTTCATATTGACTGTATATGATTTTCTTTTTTCAACCTGTTGAt                                                                           >  2:4847174/1‑140 (MQ=255)
                                                                aGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGAt                                                                           <  2:1372430/140‑1 (MQ=255)
                                                                aGACATGGTCGTTTTTTTGATGCGGGATGACGTGTAATGGCATTTTTTGTTTTTAAATAAAGTGTCTATCGTTTGCAGCTGCTGTTTGGTTAACCCCTTCATATTGACTGTATATGATCTGGTTTTTTAAACCTGTTGAt                                                                           >  2:4045982/1‑140 (MQ=255)
                                                                    aTGGTAGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACt                                                                       <  1:3201001/140‑1 (MQ=255)
                                                                      ggTCGTTTTTTTTTTGCTGGATGACGTGTAATTCCATTTTTTCTTTTTAAAGAAAGTGTGTATCTTTTTGAGCTGCTGTTTGGTTAAACCCTTAATATTGAATGTATATGATTTGGTTTTTTAAAACTGTTGATAACTGc                                                                     >  2:699969/1‑140 (MQ=255)
                                                                      ggTCGTTTTTTTGTTGGAGGCTGACGTGTAATTCCATTTTTTCTTTTTAAAGAAAGTGTGTATGGTTTTCAGGGTGAGTTTCGTTTAGCCCGTAATATTCACTGTATATTCTGTGCGTTTTTAAACCGGGTGAttaaggc                                                                     >  2:5525497/1‑134 (MQ=255)
                                                                      ggTCGTTTTTTTGATGGTGGATGACGTGTAATGCCATTTTTTGTTTTTAAAGAAAGTGTCTATCTTTTTCAGCGGCTGTTTGGTTAACACCTACATATTCACTCTATATTATTTGCTTTTTTCAACCTTTTGATAACTGc                                                                     >  2:2908695/1‑140 (MQ=255)
                                                                      ggTCGTGTTTTTTTTGCTGGATGACGTGTAATGCCCATTTTTGTTTTTAAAGAAATTGTGTAAGTTTTTCAGCTTCTGTTTCGTTAACCCCATAATATTAACGGTAAATTATTTGGTTTTTTAAACCTGGTGTTAACAGc                                                                     >  2:3941338/1‑140 (MQ=255)
                                                                       gTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGcc                                                                    <  1:1834545/140‑1 (MQ=255)
                                                                               tttGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGc                                                            <  1:5177468/140‑1 (MQ=255)
                                                                                    tGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTg                                                       <  2:1252270/140‑1 (MQ=255)
                                                                                              cGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGa                                             >  2:1126509/1‑140 (MQ=255)
                                                                                                tgtAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGc                                                      <  2:3530333/129‑1 (MQ=255)
                                                                                                       ccATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGa                                    >  2:520203/1‑140 (MQ=255)
                                                                                                       ccATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGa                                    <  1:1536883/140‑1 (MQ=255)
                                                                                                        cATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGAt                                   >  2:5614083/1‑140 (MQ=255)
                                                                                                         aTTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATg                                  <  1:882417/140‑1 (MQ=255)
                                                                                                         aTTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATg                                  <  2:2984527/140‑1 (MQ=255)
                                                                                                         aTTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATg                                  <  2:500319/140‑1 (MQ=255)
                                                                                                         aTTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATg                                  <  2:7717349/140‑1 (MQ=255)
                                                                                                         aTTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAGTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATg                                  <  1:469910/140‑1 (MQ=255)
                                                                                                          ttttttCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATgg                                 >  2:2830506/1‑140 (MQ=255)
                                                                                                                cTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCt                           <  1:1183710/140‑1 (MQ=255)
                                                                                                                cTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCt                           <  1:6639384/140‑1 (MQ=255)
                                                                                                                   tttAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCa                        <  1:4847174/140‑1 (MQ=255)
                                                                                                                   tttAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCa                        <  1:7490888/140‑1 (MQ=255)
                                                                                                                   tttAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCa                        <  1:4128063/140‑1 (MQ=255)
                                                                                                                   tttAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCa                        <  1:5377704/140‑1 (MQ=255)
                                                                                                                     tAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCACt                      <  2:1595223/140‑1 (MQ=255)
                                                                                                                     tAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCACt                      <  1:7098610/140‑1 (MQ=255)
                                                                                                                     tAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCACt                      <  1:2158805/140‑1 (MQ=255)
                                                                                                                             gtgtCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCACTGTATTCAg              <  2:6123340/140‑1 (MQ=255)
                                                                                                                              tgtCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCACTGTATTCAGc             >  2:585868/1‑140 (MQ=255)
                                                                                                                               gtCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCACTGTATTCAGcc            >  1:3599012/1‑140 (MQ=255)
                                                                                                                               gtCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCACTGTATTCAGcc            >  1:6940813/1‑140 (MQ=255)
                                                                                                                                 cTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCACTGTATTCAGCCCt          <  2:3864085/140‑1 (MQ=255)
                                                                                                                                 cTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACATGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCACTGTATTCAGCCCt          <  2:275887/140‑1 (MQ=255)
                                                                                                                                  tataTTTTGCAGCTGCTGTTTCGTTAAAAAAAAAATAAAAAATTTATATGATTTGATTTTGTCAACCTGTTGATAAATGCCTGATACTCCGGTGCTTTTCTTGATTTTTCTGATTTCGTACTTCACTGTATTAAgacatt         >  2:6374598/1‑135 (MQ=255)
                                                                                                                                   aTCTTTTGCAGCGGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCACTGTATTCAGCCCttt        >  2:7664672/1‑140 (MQ=255)
                                                                                                                                        ttGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATTTGCTTTTGTCAACATGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCACTGTATTCAGCCCTTTGAAAt   >  1:6521598/1‑140 (MQ=255)
                                                                                                                                        ttGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCACTGTATTCAGCCCTTTGAAAt   >  1:4419360/1‑140 (MQ=255)
                                                                                                                                        ttGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTGGATTTGGCTGATTGCGTCCTTCACTGTATTCAGCGCTTTGAAAt   >  1:7720222/1‑140 (MQ=255)
                                                                                                                                        ttGCAGCTGCTGTTTCGTTAACCCCTACATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGTTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCACTGTATTCAGCCCTTTGaaa  >  2:3396149/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                                          
AGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCACTGTATTCAGCCCTTTGAAATA  >  NZ_CP034943/3496302‑3496578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: