Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,498,703 G→T 7.1% intergenic (‑198/‑69) manA ← / → EO946_RS18005 mannose‑6‑phosphate isomerase, class I/spore germination protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,498,7030GT 7.1% 1102.7 / 9.5 456intergenic (‑198/‑69)manA/EO946_RS18005mannose‑6‑phosphate isomerase, class I/spore germination protein
Reads supporting (aligned to +/- strand):  ref base G (244/147);  new base T (9/46);  total (254/202)
Fisher's exact test for biased strand distribution p-value = 1.05e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 469 total aligned reads displayed.

CAAACGCCGGATAAGCTGTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATC  >  NZ_CP034943/3498564‑3498843
                                                                                                                                           |                                                                                                                                            
cAAACGCCGGATAAGCTGTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATgaag                                                                                                                                              <  1:69281/140‑1 (MQ=255)
   aCGCCGGATAAGCTGTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGaat                                                                                                                                           >  1:6395847/1‑140 (MQ=255)
    cGCCGGATAAGCTGTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGaata                                                                                                                                          >  2:5574739/1‑140 (MQ=255)
    cGCCGGATAAGCTGTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGaata                                                                                                                                          >  2:7219569/1‑140 (MQ=255)
    cGCCGGATAAGCTGTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGaata                                                                                                                                          >  2:1731415/1‑140 (MQ=255)
    cGCCGGATAAGCTGTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGaata                                                                                                                                          >  1:7188411/1‑140 (MQ=255)
    cGCCGGATAAGCTGTCTTTTTCCTATGACAACATATGTACAAAGCAGTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGaata                                                                                                                                          >  2:577731/1‑140 (MQ=255)
    cGCCGGATAAGCTGTCTTTTTCCTATGACAACATATGTAAAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCGTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAataata                                                                                                                                          >  1:1747884/1‑140 (MQ=255)
         gATAAGCTGTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATtct                                                                                                                                     >  2:4925508/1‑140 (MQ=255)
         gATAAGCTGTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATtct                                                                                                                                     >  1:2546140/1‑140 (MQ=255)
                tGTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCttttt                                                                                                                              <  1:3655028/140‑1 (MQ=255)
                            aTGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTa                                                                                                                  >  2:2372784/1‑140 (MQ=255)
                            aTGACAACATATGTACAAAGCATTATGTATCAATGACCCCATTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGACTTTTCCTTATTAAGAATAATTCTCCTTTTTTTATACAAATTa                                                                                                                  >  2:4703597/1‑140 (MQ=255)
                                 aacaTATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGaaa                                                                                                             <  1:1058240/140‑1 (MQ=255)
                                         tACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCa                                                                                                     >  2:1115744/1‑140 (MQ=255)
                                         tACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACAGTCa                                                                                                     >  2:2422974/1‑140 (MQ=255)
                                           cAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAAt                                                                                                   <  2:3186329/140‑1 (MQ=255)
                                           cAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAAt                                                                                                   <  2:1645423/140‑1 (MQ=255)
                                           cAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAAt                                                                                                   <  1:4678217/140‑1 (MQ=255)
                                           cAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAAt                                                                                                   <  1:4079009/140‑1 (MQ=255)
                                           cAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAAt                                                                                                   <  2:7398291/140‑1 (MQ=255)
                                           cAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCATTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAAt                                                                                                   <  2:6508718/140‑1 (MQ=255)
                                           cAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTGTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAAt                                                                                                   <  1:5423736/140‑1 (MQ=255)
                                           cAAAGCATTATGTATCAATGACCCCTTGTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCATCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAAt                                                                                                   <  2:1179863/140‑1 (MQ=255)
                                           cAAAGCATTATGTATCAATGACCCCTATTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAAt                                                                                                   <  1:766111/140‑1 (MQ=255)
                                             aaGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCt                                                                                                 >  1:6594590/1‑140 (MQ=255)
                                              aGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTg                                                                                                >  1:3839680/1‑140 (MQ=255)
                                              aGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTg                                                                                                >  2:6423406/1‑140 (MQ=255)
                                              aGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTg                                                                                                >  1:5840145/1‑140 (MQ=255)
                                              aGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTg                                                                                                >  1:5046959/1‑140 (MQ=255)
                                              aGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTg                                                                                                >  2:5702337/1‑140 (MQ=255)
                                              aGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTg                                                                                                >  1:1418987/1‑140 (MQ=255)
                                              aGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATATg                                                                                                >  2:3989664/1‑140 (MQ=255)
                                              aGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAAACGTCAATCTg                                                                                                >  2:7047292/1‑140 (MQ=255)
                                              aGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCATTTTTTGATACAAATTATGAAAAAAAGTCACTCTg                                                                                                >  1:4589088/1‑140 (MQ=255)
                                              aGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATAACTTTCAATATAAGGTTTGTTTCAGACTTTTACTTATGAATAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAAACTg                                                                                                >  2:5969010/1‑140 (MQ=255)
                                              aGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATAACTTTCAATAGAACGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTTATACAAATTAAGAAAAACCGTCAATCTg                                                                                                >  2:5358636/1‑140 (MQ=255)
                                                cATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTAGATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGtt                                                                                              <  2:1858688/140‑1 (MQ=255)
                                                   tATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATAAAAATTAAGAAAAACCGTCAATCTGTTTta                                                                                           >  2:6757586/1‑140 (MQ=255)
                                                     tGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTtata                                                                                         <  2:141434/140‑1 (MQ=255)
                                                          cAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAggg                                                                                    >  1:2469372/1‑140 (MQ=255)
                                                          cAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAggg                                                                                    >  1:722762/1‑140 (MQ=255)
                                                          cAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAggg                                                                                    >  1:2737761/1‑140 (MQ=255)
                                                          cAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAggg                                                                                    >  2:3266398/1‑140 (MQ=255)
                                                              gACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGgaga                                                                                >  1:961913/1‑140 (MQ=255)
                                                               aCCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATATAAGGTTTGTTTCAGCCTTTTCCGTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGgagag                                                                               >  2:2563250/1‑140 (MQ=255)
                                                               aCCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAAt                                                                                                   <  1:1286045/120‑1 (MQ=255)
                                                               aCCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGTtagag                                                                               >  2:7340275/1‑140 (MQ=255)
                                                               aCCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGgagag                                                                               >  1:2017387/1‑140 (MQ=255)
                                                               aCCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTATCCTTTTTTGATACAAATTAAGAAAAACCGTCAATATGTTTTATAAAGGGgagag                                                                               >  2:3346029/1‑140 (MQ=255)
                                                               aCCCCTTTTGAGAAAACCCTTCAGTTTTATTATATCACTTTCAATATAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATCCAATTTTAGAAAATCCGTCAATCTGTTTTATAAAggggcgtg                                                                               >  1:3917793/1‑136 (MQ=255)
                                                                   cTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGaaaa                                                                           >  2:6663005/1‑139 (MQ=255)
                                                                       tGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATg                                                                       <  1:7726798/140‑1 (MQ=255)
                                                                            aaccGCTTCCTTTTTATTAAACCCCTTGCAATAGAAGGTTTGTTTCAGCCTTTACCTTATGAAGAATAATTCTCCTTTTTTGATAAAAATTAACAAAAACCGTCAATCTTTTTTATAACGGGGAGAGAAACTATGCAAAt                                                                  <  2:4259944/137‑1 (MQ=255)
                                                                            aaaCGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCa                                                                                                     <  2:156824/105‑1 (MQ=255)
                                                                            aaaCGCTTCATTTTTATTATATCACTTTAAATAGAAGTTTTTTTTCAGCCTTTTCCTTATTAATAATAATTCTCGTTTTTTTATAAAAATTAAGAAAAACCGTCAATCTTTTTTATAAAGGGGAGAGAAACTATGCAAAt                                                                  <  1:6162820/140‑1 (MQ=255)
                                                                              aCGCTTCCGTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCg                                                                >  2:2699164/1‑140 (MQ=255)
                                                                                 cTTCCTTTTTATTATATCACTTTAAATAGAAGTTTTGTTTCAGCCTTTTCCTTATGAATAATAATTATCCTTTTTTGATACAAATTAAGAAAAACCGTAAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGAct                                                             <  2:6338949/140‑1 (MQ=255)
                                                                                    ccTTTTTATTTTATCACTTTAACTAGACGGTTTTTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGAAAATCGACTCTg                                                          <  1:1606008/140‑1 (MQ=255)
                                                                                    ccTTTTTATAATATCACTTACAATCGAAGGTTTTTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATAAAAATTAAGAACAACCGACAATCGTTTTTATAAAGGGCAGATAAACTATGCAAATCGACTCTg                                                          <  1:6206454/140‑1 (MQ=255)
                                                                                    catttttATTAAATCACTTACAATACAAGGTTTGTTCCACCATTTTCCTTATGAACAATAATTCACATTTTTTGATACAAATTAACAAAAACCGTAAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTg                                                          <  1:187742/138‑1 (MQ=255)
                                                                                         ttattaTATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGAtctc                                                     <  2:7717580/140‑1 (MQ=255)
                                                                                          tattatATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTcc                                                    >  2:2968578/1‑140 (MQ=255)
                                                                                            ttatATCACTTTCATTATAAGTTTTGTTTATGCATTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCATTGTTTTTTATAATGGGGAGAGAAACTTTGCAAATCGACTCTGATCTCCAg                                                  <  2:3608066/140‑1 (MQ=255)
                                                                                              ttatCACTTTCATTTGAAGTTTTGTTTCAGCCTTTTCTTTTTTAATAATATTTCTCTTTTTTTTATAAAATTTAATAAAAACCGTCAATCTTTTTTATAAAGGGTAGATAAACTTTGCAAATCGACTCTGATCTCCAGca                                                <  1:3021497/139‑1 (MQ=255)
                                                                                              atatCATTTTAAATATTAGGTTTGTTTCATCGTTTTAGTTATGATTAATAATTCTGATTTTTTTATACAATTTATGAAAAACCTTCAATGTGTTTTATAAAGGGGTGAGAAACTTTGCAAATCGACTCTGATCTCCAGca                                                <  2:2531003/140‑1 (MQ=255)
                                                                                              atatCACTTTCAATATATGTTTTGTTTCAGCCTTTTCCTTATGAATAATAATTCTCCTTTTTTGATAAAAATTAAGAAAAACCGTCAATCTTTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGca                                                <  1:3257012/140‑1 (MQ=255)
                                                                                              atatCACTTTCAATAGAAGTTTTGTTTCATCCTTTTCCTTATGAAGAATATTTATACTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGca                                                <  2:3963263/140‑1 (MQ=255)
                                                                                              atatCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAATGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGca                                                <  1:2399512/140‑1 (MQ=255)
                                                                                              atatCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAAATATGCAAATCGACTCTGATCTCCAGca                                                <  2:7572400/140‑1 (MQ=255)
                                                                                              atatCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAACAATAATTCTCATTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGca                                                <  1:7480973/140‑1 (MQ=255)
                                                                                              atatCACTTTCAATAGAAGGTTTGTTTCAGCATTTTCCTTATGCATAATAATTCTCTTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGca                                                <  1:5321802/140‑1 (MQ=255)
                                                                                              atatCAATTTCAATCTAAGGTTTGTTTCAGACTTTTCCTTATGAAGAATAATTCTCCTTTTTTCATAAAAATTAACAAAAACCGTCAATCGTTTTTATAAAGGGTAGAGAAACTATGAAAATCGACTCTGATCTCCAGca                                                <  1:3103957/140‑1 (MQ=255)
                                                                                              atatCAATTTAATTATATGTTTTTTTTCAGCCTTTTCCTTATGAATAATAATTCTCCTTTTTTCATAAAAATTAAGAAAAACCGTCAATCTTTTTTATAAAGGGGAGAGAAACTATGAAAATCGACTCTGATCTCCAGca                                                <  1:2333175/140‑1 (MQ=255)
                                                                                               tataACTTTCAATATAAGTTTTGTTTCAGCCTTTTCATTATTATTAATATTTCTCTATTTTTTATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGcac                                               <  2:4560120/140‑1 (MQ=255)
                                                                                               tatCACTTTCAATATAAGGTTTGTTTCAGCCTTGTCCTTATGAATAATAATTCTCCTTTTTTTATACAAGTTAAGAAAAACCGTCAATCTGTTTTATAAAGGGTAGAGAAACTATGAAAATCGAATCTGATCTCCCGcac                                               >  2:826372/1‑140 (MQ=255)
                                                                                               tatCACTTTCAATAGAAGTTTTTTTTCAGCCTTTTCCTTATTAAGAATATTTCTCCTTTTTTTATACAAATTAATAAAAACCGTCATTCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGcac                                               <  2:5026226/140‑1 (MQ=255)
                                                                                               tatCAATTTCAATAGAAGTTTTTTTTCATCATTTTCCTTATTAATAATATTTATCCTTTTTTGATACAAATTAAGAAAAACCGTCAATGTGTTTTATAAAGGGGACAGAAACTATGCAAATCGACTCTGATCTCCAGcac                                               <  2:3075129/140‑1 (MQ=255)
                                                                                               tatCAATTTAAATATAAGGTTTGTTTCAGCCTTTTCCTTATGAATAATAATTCTCCTTTTTTGATACAATTTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGATAAACTATGCAAATCGACTCTGATCTCCAGcac                                               <  2:5934586/140‑1 (MQ=255)
                                                                                                    cTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAatat                                          >  2:6118932/1‑140 (MQ=255)
                                                                                                       tCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGca                                                >  2:37746/1‑131 (MQ=255)
                                                                                                             gAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACAtt                                 >  2:2069131/1‑140 (MQ=255)
                                                                                                             gAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATATCAAGCACAATAAAGAAACAtt                                 >  2:5245890/1‑140 (MQ=255)
                                                                                                                ggTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACAATGaa                              >  2:355792/1‑140 (MQ=255)
                                                                                                                   ttgtttCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAata                                           <  2:6888109/124‑1 (MQ=255)
                                                                                                                   ttgtttCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAAACGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGaaaaa                           >  2:6804430/1‑140 (MQ=255)
                                                                                                                            ccTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTggg                  >  1:4297907/1‑140 (MQ=255)
                                                                                                                            ccTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTggg                  >  1:6063411/1‑140 (MQ=255)
                                                                                                                              ttttCCTTATGAACAATAATTCACCTTTTTTGATAAAAATTAACAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGc                <  1:5144802/140‑1 (MQ=255)
                                                                                                                                ttCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCaa              >  2:17690/1‑140 (MQ=255)
                                                                                                                                 tCCTTATGAAGAATAATTCTCCTTTTTTGATACACATTAAGAAAAACAGTCAATCTGTTGTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGCAACATGGAAAACAACATTGGGGCaaa             >  1:7386669/1‑140 (MQ=255)
                                                                                                                                   ctaaTGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGATAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAAt           <  1:7522774/137‑1 (MQ=255)
                                                                                                                                     ttgtaagaaTAATTCTCCTTTTTTCATAAAAATTAAGAAAAACGGTCAATGGGTTTTTTAAAGGGTTTATAACCTATGCAAATCGACTCTGATCTCCAGCACAATATATAAACATTGAAAAAAAAATTGGGGCAAAATGa         <  1:3534605/136‑1 (MQ=255)
                                                                                                                                     tATTAAGAATAATTCTCATTTTTTGATAAAAATTAAGAAAAACCTTCAATCTTTTTTATAAAGGGGAGAGAAACTATGAAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGa         <  1:1692118/140‑1 (MQ=255)
                                                                                                                                     tATGAAGAATATTTCTACTTTTTTTATAAAAATTAATAAAAACGTTAAATCTGTTTTATAAAGGGGAGAGAAACTATGAAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGa         <  2:5165913/140‑1 (MQ=255)
                                                                                                                                      aTGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAGGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAAttag        >  1:7584452/1‑137 (MQ=255)
                                                                                                                                      aTGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGAc        >  1:6745058/1‑140 (MQ=255)
                                                                                                                                       tGAATAATAATTCTCCTTTTTTTATACAAATTAATAAAAACCGTCAATATGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACg       <  1:838896/140‑1 (MQ=255)
                                                                                                                                       tGAAGAATAATTCTCCTTTTTTTATAAAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAACTCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACg       <  1:4815559/140‑1 (MQ=255)
                                                                                                                                        gaagaaTAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGa      <  1:5702337/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
CAAACGCCGGATAAGCTGTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTGAAAAAAAAATTGGGGCAAAATGACGATATC  >  NZ_CP034943/3498564‑3498843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: