Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,569,139 C→T 83.1% M166I (ATG→ATA mbl ← cell shape‑determining protein Mbl

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,569,1390CT83.1% 1324.6 / 234.4 537M166I (ATG→ATAmblcell shape‑determining protein Mbl
Reads supporting (aligned to +/- strand):  ref base C (36/55);  new base T (207/239);  total (243/294)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 543 total aligned reads displayed.

GTTGCGACTTTAACCTTAATATCTTCTGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCCATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCT  >  NZ_CP034943/3569002‑3569276
                                                                                                                                         |                                                                                                                                         
gTTGCGACTTTAACCTTAATATCTTCTGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTAt                                                                                                                                         <  1:1850803/140‑1 (MQ=255)
gTTGCGACTTTAACCTTAATATCTTCTGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCCAt                                                                                                                                         <  2:6019091/140‑1 (MQ=255)
  tGCGACTTTAACCTTAATATCTTCTGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGAGAATATCGCCCATTg                                                                                                                                       >  1:6451350/1‑140 (MQ=255)
  tGCGACTTTAACCTTAATATCTTCTGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTg                                                                                                                                       >  1:6856680/1‑140 (MQ=255)
  tGCGACTTTAACCTTAATATCTTCTGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCCATTg                                                                                                                                       >  1:4658099/1‑140 (MQ=255)
         ttAACCTTAATATCTTCTGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCCATTGAAATAAc                                                                                                                                <  2:2818796/140‑1 (MQ=255)
             ccTTAATATCTTCTGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACcgcg                                                                                                                            <  2:2953150/140‑1 (MQ=255)
                tAATATCTTCTGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGata                                                                                                                         >  1:4857215/1‑140 (MQ=255)
                 aagatCTTCTGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGatat                                                                                                                        <  1:1305649/137‑1 (MQ=255)
                 aaTATCTTCTGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGatat                                                                                                                        >  1:1124261/1‑140 (MQ=255)
                 aaTATCTTCTGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGAGGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATCGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGatat                                                                                                                        >  1:3610134/1‑140 (MQ=255)
                     tcttctGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCcgt                                                                                                                    <  1:2801423/140‑1 (MQ=255)
                        tctGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCcgtcgt                                                                                                                 >  1:3852770/1‑140 (MQ=255)
                        tctGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCcgtcgt                                                                                                                 >  2:1295172/1‑140 (MQ=255)
                        tctGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCcgtcgt                                                                                                                 >  1:5965655/1‑140 (MQ=255)
                        tctGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCcgtcgt                                                                                                                 >  1:7320929/1‑140 (MQ=255)
                        tctGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCcgtcgt                                                                                                                 >  1:7616892/1‑140 (MQ=255)
                        tctGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCcgtcgt                                                                                                                 >  2:7279820/1‑140 (MQ=255)
                        tctGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCcgtcgt                                                                                                                 >  1:251777/1‑140 (MQ=255)
                        tctGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAAACGCGATATCcgtcgt                                                                                                                 >  1:6365700/1‑140 (MQ=255)
                         ctGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTACTTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCgtcgtc                                                                                                                >  2:5102798/1‑140 (MQ=255)
                          tGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTcc                                                                                                               <  1:3737640/140‑1 (MQ=255)
                          tGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTcc                                                                                                               <  1:2968959/140‑1 (MQ=255)
                          tGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCCATTGAAATAACCGCGATATCCGTCGTcc                                                                                                               <  1:4181928/140‑1 (MQ=255)
                          tGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCCATTGAAATAACCGCGATATCCGTCGTcc                                                                                                               <  1:5019313/140‑1 (MQ=255)
                           gCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTccc                                                                                                              >  1:223051/1‑140 (MQ=255)
                            cAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCg                                                                                                             >  2:6735790/1‑140 (MQ=255)
                            cAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCCATTGAAATAACCGCGATATCCGTCGTCCCg                                                                                                             >  2:5420863/1‑140 (MQ=255)
                               tACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCt                                                                                                          >  1:6069767/1‑140 (MQ=255)
                                  gTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCg                                                                                                       <  2:3987461/140‑1 (MQ=255)
                                  gTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCg                                                                                                       <  1:5168509/140‑1 (MQ=255)
                                  gTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCg                                                                                                       <  1:1702779/140‑1 (MQ=255)
                                  gTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCCATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCg                                                                                                       <  1:6683504/140‑1 (MQ=255)
                                  gTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCCATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCg                                                                                                       <  1:3281260/140‑1 (MQ=255)
                                  gTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACGTGTCCCCAGCCATTTTAATAGCAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCg                                                                                                       <  1:6927633/140‑1 (MQ=255)
                                    tCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGAt                                                                                                     >  2:1946517/1‑140 (MQ=255)
                                    tCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGAt                                                                                                     <  1:4753806/140‑1 (MQ=255)
                                    tCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGAt                                                                                                     <  1:1052202/140‑1 (MQ=255)
                                    tCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGAt                                                                                                     >  2:6458784/1‑140 (MQ=255)
                                      gCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCCATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGt                                                                                                   >  2:5233074/1‑140 (MQ=255)
                                      gCCGAACAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCCATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGt                                                                                                   <  2:5314874/140‑1 (MQ=255)
                                          aTCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTAc                                                                                               <  2:2528349/140‑1 (MQ=255)
                                           tCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCCATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACa                                                                                              >  2:1587876/1‑140 (MQ=255)
                                                  cTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGt                                                                                       >  2:6382169/1‑140 (MQ=255)
                                                    tGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTa                                                                                     >  1:6503223/1‑140 (MQ=255)
                                                        cTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGc                                                                                 <  2:3320082/140‑1 (MQ=255)
                                                             gTTTGATGTAATTTAAGATTTCCTTGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCCATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGc                                                                            <  2:6224753/140‑1 (MQ=255)
                                                             gTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTAc                                                                                               >  1:3444948/1‑121 (MQ=255)
                                                             gTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGc                                                                            <  2:6162161/140‑1 (MQ=255)
                                                             gTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGc                                                                            <  2:5508727/140‑1 (MQ=255)
                                                                tGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTgg                                                                         >  1:5546422/1‑140 (MQ=255)
                                                                  aTGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGaa                                                                       <  2:1124261/140‑1 (MQ=255)
                                                                   tGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAat                                                                      <  1:1582553/140‑1 (MQ=255)
                                                                   tGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAat                                                                      <  2:3181515/140‑1 (MQ=255)
                                                                   tGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCCATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAat                                                                      <  1:4358831/140‑1 (MQ=255)
                                                                   tGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCCATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAat                                                                      <  1:2684765/140‑1 (MQ=255)
                                                                   tGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAAGAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAat                                                                      <  1:4513684/140‑1 (MQ=255)
                                                                     taaatgaaGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGAGGTCTACACCCATGTTACCGCTTGGCTGGAatat                                                                    <  1:4073313/134‑1 (MQ=255)
                                                                     tAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAatat                                                                    <  2:4132834/140‑1 (MQ=255)
                                                                       aTTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATAttt                                                                  >  2:27131/1‑140 (MQ=255)
                                                                                 tCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCCATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCcgc                                                        >  1:2284932/1‑140 (MQ=255)
                                                                                            ctggTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCgg                                             <  2:4994951/137‑1 (MQ=255)
                                                                                            cTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCgg                                             <  1:1428153/140‑1 (MQ=255)
                                                                                            cTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCgg                                             <  1:4470941/140‑1 (MQ=255)
                                                                                            cTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCCATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCAGGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCgg                                             <  2:3709015/140‑1 (MQ=255)
                                                                                             ttGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGTCTGGAATATTTCCATACCCGCGCCGATAGCGGc                                            <  2:2477982/140‑1 (MQ=255)
                                                                                             ttGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGc                                            <  1:5696293/140‑1 (MQ=255)
                                                                                               gtcaacAGCAATTTTAATAGAAGATGACTTTAAAATATCTCCCATTGAAATAACAGCGATATCCGTCGTCCCGCCTCCGAAGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATAACCGCGCCGATAGCGTCaa                                          >  2:2073419/6‑140 (MQ=255)
                                                                                                    cAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTa                                     <  2:3744164/140‑1 (MQ=255)
                                                                                                     aGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAat                                                                      >  1:5100987/1‑106 (MQ=255)
                                                                                                     aGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCCATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAg                                    >  2:7037674/1‑140 (MQ=255)
                                                                                                        caatatAATAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATGTCCATACCCGCGCCGATAGCGGCAACTTTAGGtt                                 <  2:921938/135‑1 (MQ=255)
                                                                                                        cATTTTAATAGAAGCGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGtt                                 <  2:4388741/140‑1 (MQ=255)
                                                                                                                tAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATCGCGGCAACTTTAGGTTCTTCTTCa                         <  1:7699555/140‑1 (MQ=255)
                                                                                                                tAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCa                         <  2:4250819/140‑1 (MQ=255)
                                                                                                                tAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCa                         <  2:4648361/140‑1 (MQ=255)
                                                                                                                tAGAAGAGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCa                         <  2:7552111/140‑1 (MQ=255)
                                                                                                                tAGAAGAGGAGGTGACAATATCGCCCATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACTACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCa                         <  2:1235285/140‑1 (MQ=255)
                                                                                                                tAGAAGAGGAGGTGACAATATCGCATATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCa                         <  1:2083348/140‑1 (MQ=255)
                                                                                                                tAGAAGACGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCCTGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCa                         <  1:720921/140‑1 (MQ=255)
                                                                                                                   aagaGGAGGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAgg                      >  2:3569802/1‑140 (MQ=255)
                                                                                                                     gaggagGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAat                                                                      <  1:5269536/90‑1 (MQ=255)
                                                                                                                     gaggagGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGACTATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTa                    >  2:7467397/1‑140 (MQ=255)
                                                                                                                     gaggagGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTa                    >  2:6571687/1‑140 (MQ=255)
                                                                                                                     gaggagGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTa                    <  1:7087203/140‑1 (MQ=255)
                                                                                                                     gaggagGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTa                    >  2:2182676/1‑140 (MQ=255)
                                                                                                                     gaggagGTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTa                    <  2:4808219/140‑1 (MQ=255)
                                                                                                                       ggaggTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCGTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTaca                  <  2:2342571/140‑1 (MQ=255)
                                                                                                                       ggaggTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCAGGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTaca                  <  2:790213/140‑1 (MQ=255)
                                                                                                                        gaggTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGACTACAACCATGTTACCGCTAGGCTGGAATATTTCCATACACGCGCCGATAGCGGCAACTTTAGGTTCATCTTCAAGGTacac                 >  2:4335326/1‑140 (MQ=255)
                                                                                                                           gTGACAATATCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATg              >  2:6942571/1‑140 (MQ=255)
                                                                                                                                  tatCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTAccg       >  1:796307/1‑140 (MQ=255)
                                                                                                                                  tatCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTAccg       >  2:5790866/1‑140 (MQ=255)
                                                                                                                                  tatCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTAccg       >  2:7659486/1‑140 (MQ=255)
                                                                                                                                  tatCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTAccg       >  2:4553287/1‑140 (MQ=255)
                                                                                                                                  tatCGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTGTAGGTTCTTCTTCAAGGTACACATGTTTAccg       >  2:1029197/1‑140 (MQ=255)
                                                                                                                                     cGCCTATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTAccgccg    >  2:1384398/1‑140 (MQ=255)
                                                                                                                                      gCCCATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACcgccgc   >  2:1704043/1‑140 (MQ=255)
                                                                                                                                       cccATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCt  >  2:1485661/1‑140 (MQ=255)
                                                                                                                                       cccATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCt  >  2:1837319/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                         
GTTGCGACTTTAACCTTAATATCTTCTGCAGTACGTTCGCCGATCAGCAGCTTGTACTCGCGTTTGATGTAATTTAAGATTTCCATGTCAAACTTGTCCCCAGCCATTTTAATAGAAGAGGAGGTGACAATATCGCCCATTGAAATAACCGCGATATCCGTCGTCCCGCCTCCGATGTCTACAACCATGTTACCGCTTGGCTGGAATATTTCCATACCCGCGCCGATAGCGGCAACTTTAGGTTCTTCTTCAAGGTACACATGTTTACCGCCGCT  >  NZ_CP034943/3569002‑3569276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: