Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,584,291 G→T 5.6% S324S (TCG→TCT EO946_RS18420 → N‑acetylneuraminate synthase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,584,2910GT 5.6% 1788.6 / 17.6 656S324S (TCG→TCTEO946_RS18420N‑acetylneuraminate synthase family protein
Reads supporting (aligned to +/- strand):  ref base G (296/293);  new base T (3/63);  total (300/356)
Fisher's exact test for biased strand distribution p-value = 1.39e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 662 total aligned reads displayed.

TGACAGCCAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTA  >  NZ_CP034943/3584152‑3584429
                                                                                                                                           |                                                                                                                                          
tgACAGCCAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCg                                                                                                                                            <  2:2372146/140‑1 (MQ=255)
 gACAGCCAACCAGAATATAAAAAAAGGAGAACTCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCACCTGCTGATTTATCGt                                                                                                                                           <  2:1610354/140‑1 (MQ=255)
 gACAGCCAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGt                                                                                                                                           >  1:4692787/1‑140 (MQ=255)
      ccAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAAc                                                                                                                                      >  1:6718530/1‑140 (MQ=255)
      ccAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTAAAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGATAAAc                                                                                                                                      >  2:4383644/1‑140 (MQ=255)
      ccAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTAAAAGACGAGGACGCATTTACTCAAGTAGCAACTGATGATTTATCGTTaaaa                                                                                                                                      >  2:7238288/1‑139 (MQ=255)
        aaCCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCa                                                                                                                                    >  2:785754/1‑140 (MQ=255)
        aaCCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCa                                                                                                                                    >  2:1907710/1‑140 (MQ=255)
        aaCCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCa                                                                                                                                    <  2:3561959/140‑1 (MQ=255)
         aCCAGAATATAAAAAAAGGAGACATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAg                                                                                                                                   <  1:4306317/140‑1 (MQ=255)
          ccAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGc                                                                                                                                  <  2:4611638/140‑1 (MQ=255)
                   aaaaaaaGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTacac                                                                                                                         >  1:5086623/1‑140 (MQ=255)
                        aaGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAg                                                                                                                                   >  2:6638769/1‑125 (MQ=255)
                        aaGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAAc                                                                                                                    >  2:910391/1‑140 (MQ=255)
                          ggAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGa                                                                                                                  >  2:533598/1‑140 (MQ=255)
                          ggAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGa                                                                                                                  >  2:1762435/1‑140 (MQ=255)
                                aTCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATaaa                                                                                                            <  1:3170635/140‑1 (MQ=255)
                                aTCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATaaa                                                                                                            <  2:2304216/140‑1 (MQ=255)
                                aTCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATaaa                                                                                                            <  2:5684659/140‑1 (MQ=255)
                                            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                >  2:4986311/1‑140 (MQ=255)
                                            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                >  2:5230676/1‑140 (MQ=255)
                                            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                >  2:6117115/1‑140 (MQ=255)
                                            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                >  1:7751117/1‑140 (MQ=255)
                                            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                >  1:1059349/1‑140 (MQ=255)
                                            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                >  1:3089094/1‑140 (MQ=255)
                                            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                >  1:3405835/1‑140 (MQ=255)
                                               aaaaCAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGc                                                                                             >  2:3721839/1‑140 (MQ=255)
                                                 aaCAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCCTTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCCACGAAATAAAAAGTCTAAGAATTGCGa                                                                                           >  2:1130006/1‑140 (MQ=255)
                                                 aaCAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTgaga                                                                                           >  2:4106394/1‑140 (MQ=255)
                                                 aaCAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGa                                                                                           >  2:2446375/1‑140 (MQ=255)
                                                    aaGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTg                                                                                        <  2:6939629/140‑1 (MQ=255)
                                                     aGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGc                                                                                       >  1:5907685/1‑140 (MQ=255)
                                                      gCTCCACAAGATGCGGAAATGAGAATGCATTAATTCCTTTACAATCCGAGGACTCATTTCCTCAAGTAGCAACTGCTGATTTATCCTTAAACCAGCCCATTACACCCAACGAAAAAAAAAGTCTAAGAAATGCGATTCCa                                                                                      >  2:7359059/1‑140 (MQ=255)
                                                      gCTCCAAAAGATGCGGAACTGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                      >  1:299722/1‑140 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAATCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACAACCAACGAAATAAAAAGTCTAAGACTTGCGATTGCa                                                                                      >  1:3858399/1‑140 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                      <  2:5533345/140‑1 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                      >  2:2601335/1‑140 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                      >  2:2212565/1‑140 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                      <  1:2483127/140‑1 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                      <  1:910391/140‑1 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                      >  1:4064550/1‑140 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                      >  1:7095699/1‑140 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                      >  1:6841114/1‑140 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAACAATTGCGATTGCa                                                                                      >  2:449158/1‑140 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCAATACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                      >  2:716821/1‑140 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAg                                                                                     >  2:3452834/1‑140 (MQ=255)
                                                        tCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGt                                                                                    <  1:6500343/140‑1 (MQ=255)
                                                          cAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGa                                                                                  >  2:1263687/1‑140 (MQ=255)
                                                                  gCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAgg                                                                          >  2:7448853/1‑140 (MQ=255)
                                                                    ggAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTGTCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCt                                                                        >  2:416842/1‑140 (MQ=255)
                                                                                         ccTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTTCAGTGATATGTAGGCTAAAATCGACTAGACTTCCGaa                                                   >  2:5871326/1‑140 (MQ=255)
                                                                                           tttACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCAAAAATCGACTCGTCTGCCGaaaa                                                 >  2:6744892/1‑140 (MQ=255)
                                                                                           tttACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACACAACGAAATAAAAAGTCTAAGAATTGCGATAGCAGTGATATGTAGGCTAAAATCGAATCTTCTGCCGaaca                                                 >  2:6247070/1‑138 (MQ=255)
                                                                                            ttACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGaaaaa                                                >  1:5247570/1‑140 (MQ=255)
                                                                                              aCAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAg                                              >  2:1047380/1‑140 (MQ=255)
                                                                                                  gCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCgct                                          >  1:7416920/1‑140 (MQ=255)
                                                                                                   ccGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCATTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAATCgctg                                         >  2:3212761/1‑140 (MQ=255)
                                                                                                   ccGAGGACGCATTTCCACAAGTAGCAACTGCTGATTTATCGTTAAACCACCCCATTACACCCACCGAAAAAAAAAGAATAAGAATTGCGATTGCAGTGATATGTAGGCTAAACTCGACTCGTCTGCCGAAAAAAGCgctg                                         <  1:1677241/140‑1 (MQ=255)
                                                                                                    cGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCgctgc                                        >  1:1422037/1‑140 (MQ=255)
                                                                                                      cggACGCATTTCCTCACGTAGCAACTGCTGATTTATCGTTAAACCAGCCAATTACACCCACCGAAAAAAACAGTCTAAGAATTTCGATTGCAGTCTTTTTTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTc                                      <  2:752852/139‑1 (MQ=255)
                                                                                                      atgACGCTTTTGCTAAATTAGCAGCTGATTTTTTATTTTTAAACACGCCAATTACACCAAACTAATTAAAAAGTCTAATAATTGCTTTTGCATTTTTTTGTAGGGTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTc                                      <  1:270176/138‑1 (MQ=255)
                                                                                                      aGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAATTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTc                                      >  1:1277711/1‑140 (MQ=255)
                                                                                                      aGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTc                                      <  2:7080625/140‑1 (MQ=255)
                                                                                                      aGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTc                                      >  2:7658019/1‑140 (MQ=255)
                                                                                                      aGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTc                                      <  2:4893138/140‑1 (MQ=255)
                                                                                                      aGGACGCATTTCCTAAATTAGCAACTGCTGATTTATCTTTAAACCAGCCCTTTACACCAAAGGAAATAAAAAGTCTAATAATTGCGATTGCTTTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTc                                      <  1:6117115/140‑1 (MQ=255)
                                                                                                      aGGACGAATTTCCTCAAGTAGAAACTGCTTATTTAACGTTAAACCACCAAATTACACCCAACCAATTAAAAAGTCTAATAATTCCGATTGCAGTGATATGTAGTCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTc                                      <  1:3721839/140‑1 (MQ=255)
                                                                                                       ggACTCATTTCCTAAAGTAGAAACTGGTTTTTTTTCTTTAAACCAGCCCATTACACCCAACGAAAAAAAAAGTATAATATTTGCGATTGCAGTTATATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTcc                                     <  1:2392125/140‑1 (MQ=255)
                                                                                                       ggACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCCGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTcc                                     <  2:3872717/140‑1 (MQ=255)
                                                                                                       ggACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCTATTGAATTGATATGTAGGCTAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTcc                                     <  1:349689/140‑1 (MQ=255)
                                                                                                         aCGAATTTCCTAAAGTAGCAACTGCTTATTTATCTTTAAACCAGCCCATTACACCCAACTAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCt                                   <  2:6820147/140‑1 (MQ=255)
                                                                                                           tcATTTCCTAAAGTAGAAACTGCTTATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAt                                 <  2:2009167/139‑1 (MQ=255)
                                                                                                           gaaTTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCACCCAATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGTTTGCAGTGATATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAt                                 <  1:4816192/138‑1 (MQ=255)
                                                                                                           gCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGGCTAAGATTTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAt                                 <  2:2845342/140‑1 (MQ=255)
                                                                                                           gCATTTCATCAAGTAGCAACTGCTGTTTTATCGTAAAACCACCCCTTTACACCCAACGAAATAAAACGTCTAAGAATTGCGATTGCAGTTATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAt                                 <  2:3092008/140‑1 (MQ=255)
                                                                                                             aTTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGAGATTGCAGTGATATGTAGGCTAAAATCGACTCTTCTGCAGAAAAAAGCTCTTCTCACTAttt                               >  2:6486987/1‑140 (MQ=255)
                                                                                                              tttCCTCAAGTAGCAACTGCTTATTTATCTTTAAACCAGCCCATTACACCCAACGAAATAAAACGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTa                              <  1:5437071/140‑1 (MQ=255)
                                                                                                              tttCCTCAAGTAGCAACTGATGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTa                              <  1:4463053/140‑1 (MQ=255)
                                                                                                               ttCCTCATGTAGCAAGTGCTTTTTTGTCTTTAAACCAGCCAATTACACCCAACTAAAAAAAAAGTTTAAGAATTGCGATTGCAGTTATATGTCGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTAc                             <  1:416842/140‑1 (MQ=255)
                                                                                                               ttCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTAc                             <  1:1792397/140‑1 (MQ=255)
                                                                                                               ttCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCAAACTAAATAAAACGTCAACTAATTGCGATTGCCTTGATATGTAGGATAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTAc                             <  2:5421533/140‑1 (MQ=255)
                                                                                                               ttCCTCAAGTAGAAACGGCGGATTTTACGTAAACCCACCCCATTACCCCCAACGAAATAAAAAGTCTACTAATGGCGTTTGCCGTGATTTGTTGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTAc                             <  1:7713413/140‑1 (MQ=255)
                                                                                                               ttCCTAAAGTAGCAACTGCTGATTTATCGTTAAACCACCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTAc                             <  2:4822960/140‑1 (MQ=255)
                                                                                                               ttCCTAAAGTAGAAACTTCTTATTTATCGTTAAACACGCCCTTTACCCCCCACGAAATAAAAAGTCTAACAATTGCTATTGCAGTGATATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTAc                             <  1:1204614/140‑1 (MQ=255)
                                                                                                               taccTCAATTAGCAACTGCTGATTTATCGTTAAACCCGCCCAGTACACCCAACCAAATAAAAAGTCTAAGAATGGCTATTGCAGTGAAATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTAc                             <  1:3313898/138‑1 (MQ=255)
                                                                                                                  ctaaaGTAGCAACTGCTTATTTTTCTTTAAACCAGCCCATTACACCCAACGAATTAAAAAGTCTAAGATTTGCGTTTGCTGTGATATGTAGGCTAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGt                          <  2:473841/137‑1 (MQ=255)
                                                                                                                  ctaaaGTAGCAACTGCTGATTTATCGTAAAACCAGCCCATTACCCCCCACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTTATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGt                          <  2:2926897/137‑1 (MQ=255)
                                                                                                                  cTCAAGTATAAACTGCTTATTTATCTTTAAACCAGCCCATTACACCCACCCAAATAAAAAGTCTAAGAATTGCGATTGCAGTTATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGt                          <  1:6952954/140‑1 (MQ=255)
                                                                                                                  cTCAAGTAGCAAGTGCTGTTTTATGGTTAAACCAGCCCATTACACCCAACTAAATAAAAAGTCTAAGATTTGCGATTGCAGTTATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGt                          <  1:7539902/140‑1 (MQ=255)
                                                                                                                  cTCAAGTAGCAACTGCTTATTTATCGTTAAACCAGCCCATTCCACCCAACCAAATAAAAAGTCTAAGAATTGCGATTGCAGTTATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGt                          <  1:5586245/140‑1 (MQ=255)
                                                                                                                       gTAGCAACTGCTGATTTATCGTTAAACCACCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATAGGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATAcc                     <  1:5709994/140‑1 (MQ=255)
                                                                                                                         aGCAACTGCTGATTTATCTTTAAACCAGCCCATTACACCCAACTAAATAAAAAGTATAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCAt                   <  1:7196743/140‑1 (MQ=255)
                                                                                                                         aGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCAt                   <  1:2274599/140‑1 (MQ=255)
                                                                                                                         aGCAACTGCTGATTTATCGTAACACCAGCCCATTACACCCAACGAAAAAAAACGTCTACCAATTGCGTTGGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCAt                   <  1:379055/140‑1 (MQ=255)
                                                                                                                           cAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAGATCGACTCGTCTGCCGAAAAAAGCGCTGCTc                                      >  1:1029250/1‑119 (MQ=255)
                                                                                                                               tgctgATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGACGAAAAAAGCGCTGCTCACTATTTACGGTATACCATCTGTTg             >  2:3311280/1‑140 (MQ=255)
                                                                                                                                 ctgATTTATCGTTAAACCAGCCCATTACACCCACCGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTTGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAt                                 <  2:1498716/118‑1 (MQ=255)
                                                                                                                                 ctgATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGaa           >  1:6256221/1‑140 (MQ=255)
                                                                                                                                 ctgATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGaa           >  1:2113322/1‑140 (MQ=255)
                                                                                                                                 ctgATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGaa           >  1:4937889/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TGACAGCCAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTA  >  NZ_CP034943/3584152‑3584429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: