Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,790,764 T→G 13.4% N203H (AAC→CAC)  EO946_RS19525 ← BglG family transcription antiterminator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,790,7640TG13.4% 1185.4 / 62.1 488N203H (AAC→CAC) EO946_RS19525BglG family transcription antiterminator
Reads supporting (aligned to +/- strand):  ref base T (49/357);  new base G (80/2);  total (129/359)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 495 total aligned reads displayed.

TATTCTTTTTGATGCAGGATATGATCCATATCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTG  >  NZ_CP034943/3790625‑3790899
                                                                                                                                           |                                                                                                                                       
     tttttGATGCAGGATATGATCCATATCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATCGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGtgtttga                                                                                                                                    >  1:4580270/1‑138 (MQ=255)
     tttttGATGCAGGATATGATCCATATCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGtttttta                                                                                                                                    >  1:6530842/1‑138 (MQ=255)
       tttGATGCAGGATATGATCCATATCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCAtt                                                                                                                                  <  1:1147510/140‑1 (MQ=255)
       tttGATGCAGGATATGATCCATATCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGAtt                                                                                                                                  >  1:1042801/1‑140 (MQ=255)
          gATGCAGGATATGATCCATATCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCAttttt                                                                                                                               >  2:3244093/1‑140 (MQ=255)
               aGGATATGATCCATATCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAg                                                                                                                          <  2:3350699/140‑1 (MQ=255)
                 gacaggATCCATATCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGa                                                                                                                        <  1:4243866/135‑1 (MQ=255)
                 gATATGATCCATATCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTCTATTTTTTGAgta                                                                                                                        >  1:2841028/1‑138 (MQ=255)
                      gATCCATATCTTCCGGGAAATGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGATTTTTTTAAGGATAGCg                                                                                                                   >  2:5536383/1‑140 (MQ=255)
                           atatCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTAATTTTTTTTATGATGGGGGaaag                                                                                                              >  2:2857629/1‑138 (MQ=255)
                           atatCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTCATTTTTTTAAGGATGGCGGAACg                                                                                                              >  2:3595370/1‑140 (MQ=255)
                           atatCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTAAGTCCCAAGTTGGACAGAGGGATTCGGTCGTTTTTCATTTTTTTAAGTCATGCGGAACg                                                                                                              >  2:3506158/1‑140 (MQ=255)
                            tatCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGGTTGATTTTTTTGAGGGTGGCGGGACgg                                                                                                             >  1:5431748/1‑140 (MQ=255)
                             atCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGga                                                                                                            >  2:4463319/1‑140 (MQ=255)
                             atCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGATTTTTTTTAGGTTTGGGGGACGga                                                                                                            >  1:7100483/1‑140 (MQ=255)
                                 tCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGAGAGAGGGATGCGGTCGTTTTTCATTTTTTTGAGGATGGAGGAAAGgatgtt                                                                                                        >  2:3828145/1‑138 (MQ=255)
                                 tCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTTTTTTTTTTTAGGGTTGGGGGACgggatat                                                                                                        >  1:224974/1‑135 (MQ=255)
                                 tCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGATTTTTTTTAGGGTGGGGGGAAGgatgat                                                                                                        >  1:3319225/1‑140 (MQ=255)
                                 tCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGGTTTATTTTTTTGGTGCTGTCGGAAAGgattag                                                                                                        >  1:4003762/1‑137 (MQ=255)
                                  ccGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTTATTTTTTTAAGGATGGCGGCACGgatgata                                                                                                       >  2:4103995/1‑139 (MQ=255)
                                  ccGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGATTTTTTTAATGATTGGGGAAAGgatgata                                                                                                       >  2:4033762/1‑139 (MQ=255)
                                  ccGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGATTTTTTTAATGATTGCGGAACAGCTGAtc                                                                                                       >  2:7415777/1‑140 (MQ=255)
                                  ccGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGATTTTTTTAATGATGGCGGAACggcggata                                                                                                       >  2:3066685/1‑134 (MQ=255)
                                               tACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATTATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCAt                                                                                          <  2:5208499/140‑1 (MQ=255)
                                                     gTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCa                                                                                    <  1:7543651/140‑1 (MQ=255)
                                                     gTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCa                                                                                    <  2:1692706/140‑1 (MQ=255)
                                                      ttttGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTTATTTTTTTTATGATGGGGGAACGGATGATATCAATTTATTAAtttttgaa                                                                                   >  1:789744/1‑135 (MQ=255)
                                                           cGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAatat                                                                              <  1:5059509/140‑1 (MQ=255)
                                                           cGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAatat                                                                              <  1:1381296/140‑1 (MQ=255)
                                                              tCTGATCCGTTTACAGGCGATAGAGATATGGAGGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAg                                                                           <  2:157876/140‑1 (MQ=255)
                                                                tGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTGATTTTTTTCAGGATTGCGGAAACGCTGATCTCGATTTATTCAATTGGCAAAATATCAGcc                                                                         >  2:7077623/1‑140 (MQ=255)
                                                                 gATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCt                                                                        <  2:2322176/140‑1 (MQ=255)
                                                                   tCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCttt                                                                      <  1:2708523/140‑1 (MQ=255)
                                                                      gTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTttca                                                                   <  2:2417719/140‑1 (MQ=255)
                                                                        ttACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTttcatt                                                                 <  1:3618204/140‑1 (MQ=255)
                                                                                          tGGATGATGCGGTTCTTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGt                                               <  1:7422693/140‑1 (MQ=255)
                                                                                          tGGATGATGAGGTTATTCAGTGCCTAGTTGGACAGAGTGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATAAGCCTTTTCATTCAGAACATCAATCTCCGt                                               <  1:6589915/140‑1 (MQ=255)
                                                                                          tGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGt                                               <  1:1584461/140‑1 (MQ=255)
                                                                                              tgatgaGGTTATTCAGTCCCAAGTTTGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTtct                                           <  2:6022410/140‑1 (MQ=255)
                                                                                              ggatgaGGTTAGTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTtct                                           <  1:4378721/139‑1 (MQ=255)
                                                                                                  gaGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTACATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCttt                                       <  1:3510297/140‑1 (MQ=255)
                                                                                                   aGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTc                                      <  1:5484349/140‑1 (MQ=255)
                                                                                                     gTTATTCAGTCCCAATTTGTACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGCACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGACTTTTCATTCAGACCTTCAATCTCCGTTTCTCTTACAt                                    <  2:5006160/140‑1 (MQ=255)
                                                                                                     gTTATTCAGTCCCAAGTTGGACAGAGGTATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCAt                                    <  2:6965335/140‑1 (MQ=255)
                                                                                                     gTTATTCAGTCCCAAGTTGGACAGAGGGATGCGTTCGTTTTTCATTTTTTTCAGCATGGCGGAACGGATGATCTCGATGTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCAt                                    <  2:5411187/140‑1 (MQ=255)
                                                                                                     gTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTGATTTTTTTGAGGATTGCGGAACGGATGATTTCGATTTCTTGATTTTGGAAAATATCAGCCGTTTGATTCAGAAAATAAATAACCGGTTCTCTGTCag                                    >  2:1784118/1‑139 (MQ=255)
                                                                                                     gTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCAt                                    <  1:1925694/140‑1 (MQ=255)
                                                                                                     gTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCAt                                    <  1:1458508/140‑1 (MQ=255)
                                                                                                     gTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCAt                                    <  1:3088783/140‑1 (MQ=255)
                                                                                                     gTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCAt                                    <  2:3760524/140‑1 (MQ=255)
                                                                                                     gTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCAt                                    <  2:810782/140‑1 (MQ=255)
                                                                                                     gTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTAGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCAt                                    <  2:2566817/140‑1 (MQ=255)
                                                                                                      ttATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTAAATTTTTTGAGGGTGGGGGAAAGGCTGGTATAGATTTATTCATTTTGGAAAAAATCAGCCTTTTCAATAAGAACAACAAACTCCGTGTGTCTTTGAta                                   >  2:6146402/1‑139 (MQ=255)
                                                                                                       tATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTCATTTTTTTGATGTTGGCGGCACGGCTTATCTCGATTTATTTATTTTTCAAAAAATAAGCCTTTTCATTCAGAAAAACAAAATCCCGTTCTCTTTAATCg                                  >  2:3983353/1‑140 (MQ=255)
                                                                                                        aTTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGa                                 <  2:2107866/140‑1 (MQ=255)
                                                                                                          tCAGTCCCAATTTTGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATTATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACa                               <  2:1161797/140‑1 (MQ=255)
                                                                                                          tCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACa                               <  2:1395771/140‑1 (MQ=255)
                                                                                                          tCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACa                               <  2:1212628/140‑1 (MQ=255)
                                                                                                          tCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACa                               <  1:6278537/140‑1 (MQ=255)
                                                                                                          tCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTAGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACa                               <  1:2552952/140‑1 (MQ=255)
                                                                                                           cAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACaa                              <  1:3747625/140‑1 (MQ=255)
                                                                                                               cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                          <  1:210511/140‑1 (MQ=255)
                                                                                                               cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                          <  1:2448900/140‑1 (MQ=255)
                                                                                                               cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                          <  2:6864309/140‑1 (MQ=255)
                                                                                                               cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                          <  1:5825325/140‑1 (MQ=255)
                                                                                                               cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                          <  1:1780510/140‑1 (MQ=255)
                                                                                                                ccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTAc                         <  1:383922/140‑1 (MQ=255)
                                                                                                                ccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTAc                         <  1:5601845/140‑1 (MQ=255)
                                                                                                                ccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTAc                         <  1:1225865/140‑1 (MQ=255)
                                                                                                                 cAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAGCATCAATCTCCGTTTCTCTTTCATCGACAATGTACt                        <  2:6242988/140‑1 (MQ=255)
                                                                                                                 cAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACt                        <  1:3247298/140‑1 (MQ=255)
                                                                                                                 cAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACt                        <  2:5652029/140‑1 (MQ=255)
                                                                                                                  aaGTTGGACAGAGGGATGCGGTCGTGTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTc                       <  2:4323114/140‑1 (MQ=255)
                                                                                                                   aGTTGGACAGAGGGATGCGGTCGTTTTTAATTTTTTTGAGGCTGGGGGAAAGGATTATCTAGATTTGTTCATTTGTGAAAAAATAAGCCGTTTGATTGATAACAAAAATATCCCGTTGTATTTGATCGAACATGttatac                      >  2:4701408/1‑135 (MQ=255)
                                                                                                                    gTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCg                     <  2:1844359/140‑1 (MQ=255)
                                                                                                                    gTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCg                     <  2:530657/140‑1 (MQ=255)
                                                                                                                    gTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCg                     <  1:5984437/140‑1 (MQ=255)
                                                                                                                    gTTGGACAGAGGGATGCGGTCGTTTGTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCg                     <  1:4845856/140‑1 (MQ=255)
                                                                                                                    cttGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCGTCATTTGGCACACTATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCg                     <  1:1996063/139‑1 (MQ=255)
                                                                                                                      tGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTATTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGcc                   <  2:7609545/140‑1 (MQ=255)
                                                                                                                       ggACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCa                  <  1:4111951/140‑1 (MQ=255)
                                                                                                                       ggACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCa                  <  1:7666965/140‑1 (MQ=255)
                                                                                                                       ggACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCa                  <  2:5684903/140‑1 (MQ=255)
                                                                                                                       ggACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCa                  <  2:572096/140‑1 (MQ=255)
                                                                                                                       ggACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCAGTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCa                  <  1:7019566/140‑1 (MQ=255)
                                                                                                                          cAGAGGGATGCTGTCTTTTTTCATTTTTTTCAGGATGGCGTAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATAc               <  2:5857877/140‑1 (MQ=255)
                                                                                                                          cAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATAc               <  2:4850685/140‑1 (MQ=255)
                                                                                                                          cAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATAc               <  2:4580270/140‑1 (MQ=255)
                                                                                                                          cAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATAc               <  1:6373947/140‑1 (MQ=255)
                                                                                                                          cAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATAc               <  1:6823503/140‑1 (MQ=255)
                                                                                                                          cAGAGGGATGCGGTAGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATAc               <  2:883799/140‑1 (MQ=255)
                                                                                                                             agGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGt            <  2:6528329/140‑1 (MQ=255)
                                                                                                                               ggATGCGGTCTTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTAc          <  2:2494075/140‑1 (MQ=255)
                                                                                                                               ggATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTAc          <  2:1972602/140‑1 (MQ=255)
                                                                                                                               ggATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTAc          <  1:5672302/140‑1 (MQ=255)
                                                                                                                               ggATGCGGTCGTGTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACACCGCCATACAGTAc          <  2:6653373/140‑1 (MQ=255)
                                                                                                                               ggATGCGGTAGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTAc          <  2:421247/140‑1 (MQ=255)
                                                                                                                                 aTGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCt        <  1:5291369/140‑1 (MQ=255)
                                                                                                                                  tGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTc       <  1:6674501/140‑1 (MQ=255)
                                                                                                                                    cGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCAt     <  2:2714356/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                       
TATTCTTTTTGATGCAGGATATGATCCATATCTTCCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTTCATTTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTG  >  NZ_CP034943/3790625‑3790899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: