Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,846,624 G→T 6.7% Q195K (CAA→AAA)  dbpA ← ATP‑dependent RNA helicase DbpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,846,6240GT 6.7% 1160.0 / 15.4 427Q195K (CAA→AAA) dbpAATP‑dependent RNA helicase DbpA
Reads supporting (aligned to +/- strand):  ref base G (169/214);  new base T (42/2);  total (211/216)
Fisher's exact test for biased strand distribution p-value = 4.78e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 436 total aligned reads displayed.

TCGGGATTCTCCGTCATCAGCACATCTTTCAGCAAAGAAAACTTATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGACC  >  NZ_CP034943/3846485‑3846763
                                                                                                                                           |                                                                                                                                           
tCGGGATTCTCCGTCATCAGCACATCTTTCAGCAAAGAAAACTTATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTg                                                                                                                                             >  1:3878200/1‑140 (MQ=255)
tCGGGATTCTCCGTCATCAGCACATCTTTCAGCAAAGAAAACTTATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCGTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCCTATATTg                                                                                                                                             >  2:2925213/1‑140 (MQ=255)
         tcCGTCATCAGCACATCTTTCAGCAAAGAAAACTTATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCaa                                                                                                                                    <  1:1730346/140‑1 (MQ=255)
          ccGTCATCAGCACATCTTTCAGCAAAGAAAACTTATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAAt                                                                                                                                   >  2:5011299/1‑140 (MQ=255)
           cGTCATCAGCACATCTTTCAGCAAAGAAAACTTATTCTATTCTCTTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAAtt                                                                                                                                  <  2:5371700/140‑1 (MQ=255)
            gTCATCAGCACATCTTTCAGCAAAGAAAACTTATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAAttt                                                                                                                                 >  1:2738967/1‑140 (MQ=255)
              catcaGCACATCTTTCAGCAAAGAAAACTTATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTtct                                                                                                                               >  1:5580526/1‑140 (MQ=255)
                         cTTTCAGCAAAGAAAACTTATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATAtcctcc                                                                                                                    >  2:4270466/1‑140 (MQ=255)
                                cAAAGAAAACTTAGTCTCTTCGCTTACTTGAATCACCGCATGTTCAATATTATTTGTGGTTAGGCCTGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATTTATTGACGGCTCAATTTCTAAATATCCTCCGGCAGCg                                                                                                             <  2:716241/140‑1 (MQ=255)
                                   aGAAAACTTATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTGTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTGTTGCATATATTTACGGCGCAATTTCTCTATATCATCCGGCAGCGTTg                                                                                                          >  1:4507687/1‑140 (MQ=255)
                                   aGAAAACTTATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGTTTTCAATATGCTGCGGGTTTTTCATATATTGACGGCTCAATTTATAAATATCCTCCGGCAGCGTTg                                                                                                          >  1:4113437/1‑140 (MQ=255)
                                   aGAAAACTTATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCGTGGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCCTATATTTACGGCTAAATTTCTCAATATCCTCCGGCAGCCTTg                                                                                                          >  1:5128542/1‑140 (MQ=255)
                                   aGAAAACTTATTCTCTTCTCGTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTg                                                                                                          <  2:1612875/140‑1 (MQ=255)
                                     aaaaCTTATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGcc                                                                                                        <  2:7745252/140‑1 (MQ=255)
                                          ttATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGaaaa                                                                                                   <  2:3873317/140‑1 (MQ=255)
                                          ttATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGaaaa                                                                                                   <  2:4381018/140‑1 (MQ=255)
                                            aTTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGcc                                                                                                        >  2:2310907/1‑133 (MQ=255)
                                               ctcttctcttACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAacaaca                                                                                              <  2:3137072/140‑1 (MQ=255)
                                               ctcttctcttACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCGTTGATTTAAATATTCTGCGGGTTTTTCATATATTTACGGCTCAATTTCTCAATAAACTCCGGCACCGTTGCCCAAaacaaaa                                                                                              >  1:2688605/1‑138 (MQ=255)
                                                  ttctcttACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTATGCCGGCCGCTTTGATTTCAATATTCTGCGGGTTTTTCATATATTGACGGCTCAATTTCTAAATATACTCCGGCAGCGTTGCCGAAAACAACAtcg                                                                                           >  2:4475043/1‑140 (MQ=255)
                                                  ttctcttACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGTTTTCAAAATGCTGCGGGTTTTGCATAAATTTACGGCTCAATTTATCAATATACACCGGCACCGTTGCCGAAAACAACAacg                                                                                           >  1:4869842/1‑140 (MQ=255)
                                                  ttctcttACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTTCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACAtcg                                                                                           >  2:4818817/1‑140 (MQ=255)
                                                  ttctcttACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACAtcg                                                                                           >  2:1735325/1‑140 (MQ=255)
                                                  ttctcttACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACAtcg                                                                                           >  1:7585681/1‑140 (MQ=255)
                                                   tctcttACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACAtcgt                                                                                          <  1:252432/140‑1 (MQ=255)
                                                    ctcttACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTTCATATATTGACGGCTCAATTTATCAATATCCTCCGGCAGCGTTGCCGAAAACAACAtcgtc                                                                                         >  1:6192743/1‑140 (MQ=255)
                                                        tACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCACTATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTgc                                                                                     <  2:1260656/140‑1 (MQ=255)
                                                        tACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTgc                                                                                     <  1:3514345/140‑1 (MQ=255)
                                                        tACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTgc                                                                                     <  1:2796323/140‑1 (MQ=255)
                                                        tACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTgc                                                                                     <  1:365851/140‑1 (MQ=255)
                                                         aCTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTgcg                                                                                    <  2:5841200/140‑1 (MQ=255)
                                                         aCTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTgcg                                                                                    <  1:572476/140‑1 (MQ=255)
                                                           ttGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGct                                                                                  <  1:1633314/140‑1 (MQ=255)
                                                           ttGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGct                                                                                  <  1:1913566/140‑1 (MQ=255)
                                                            tGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTAAATATTCTGCGGGTTTTTGATATATTGACGGCTAAATTTATAAATATCCTCCGGCAGCGTTGCCGAAAACCACATCGTCGTgcgcgc                                                                                 >  1:2984292/1‑138 (MQ=255)
                                                              aaTCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATTCTGCGGGTTTTGGATATATTGAAGGCTCAATTTATCAAAATACTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTg                                                                               >  1:825434/1‑140 (MQ=255)
                                                              aaTCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCACTg                                                                               >  1:5541674/1‑140 (MQ=255)
                                                               aacACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTTACGGCTCAATTTCTCAATATACTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGt                                                                              >  2:2036645/3‑140 (MQ=255)
                                                               aTCACCGCATGTTCAATATTTCTTGTGGTTAGGCCGGCCGCTTTGACCTCGATATGCTCGGGGTTTTGCATATATTGACGGCTCAGCTTCTCGATATCCTGCGGAAGCGTCGCTGAAAACAGCATCGTCGTACGCTCAGt                                                                              >  2:3009983/1‑140 (MQ=255)
                                                               aTCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGt                                                                              <  1:2124226/140‑1 (MQ=255)
                                                                    cGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATTCTGCGGGGTTTGGATATATTTACGGCTCAATTTCTCAATATACTCCGGCAGCGTTGCCGAAAACAACAACGTCGTGCGCTCGGTCGGCa                                                                         >  1:6760115/1‑140 (MQ=255)
                                                                    cGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAAAATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCa                                                                         >  1:7349157/1‑140 (MQ=255)
                                                                      cATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGa                                                                       <  2:5230693/140‑1 (MQ=255)
                                                                      cATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTACGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGa                                                                       <  2:3586262/140‑1 (MQ=255)
                                                                        ttttCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATg                                                                     <  2:6419500/138‑1 (MQ=255)
                                                                        tGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGTTAAAAACATCGTCGTGCGCTCTGTCGGCAGATg                                                                     <  2:2738967/140‑1 (MQ=255)
                                                                        tGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATg                                                                     <  2:2562512/140‑1 (MQ=255)
                                                                         gTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTAAATATGCAGCGGGTTTTGCATATAATTAAGGCTCAATTTATAAAAATCATACCGCAGCGTTGCCGAAAAAAACATAGTAGTTGGCAATCTCGGCATATga                                                                    >  1:6419500/1‑139 (MQ=255)
                                                                              atatTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTAAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTAGTGCGCTCTGTAGGCAGATTCTTGAt                                                               >  2:2861632/1‑140 (MQ=255)
                                                                                 ttCTTTGTGGTTAGGCCGGCCGCTTTGATTTCACTATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAAt                                                            <  2:5525111/140‑1 (MQ=255)
                                                                                    tttGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTTCGGGTTTTGCCTATATTTAAGGATCAATTTATAAAAAACCTACGTCAGCGTTGACGAAAACAACATCTTCGTGCGCTCTGTCGGCAGATGCTTGATAATAGc                                                         >  2:3408251/1‑140 (MQ=255)
                                                                                    tttGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGc                                                         <  2:154771/140‑1 (MQ=255)
                                                                                     ttGTGGTTAGGCCGGCCGCTTTTATTTCAATATTCTGCGGGTTTTTCATATATTTACGGCACAAATTATCAATATACTCCGGCACCGTTGCCGAAAAAAACAACGTCCTGCCCTCAGTAGGCAGAAGCATGAAAAACGcc                                                        >  1:6983928/1‑140 (MQ=255)
                                                                                      tgtgGTTAGGCCGGCCGCTTTGATTTAAATATGCTGCGGGTTTTGCATATATTGAAGGATCAATTTCTCAATATCATCCGGCAGCGGTGCCGAAAACAACAACGTCGTGCGCTCTGTCCGCAGATGCTTGATAAAAGCct                                                       >  2:1874334/1‑140 (MQ=255)
                                                                                         ggTTAGGCCGGCCGCTTTGATTTCAATATTCTGCGGGTTTTGCATAAATTGACGGCACAAATTCTAAATAAACACCGGCAGCCTTGACGAAAACAAAAACGTCGTGCGCTCTTGAGGCAGATGCTTGATAATCGCCACTa                                                    >  1:6831517/1‑140 (MQ=255)
                                                                                         ggTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTa                                                    >  2:403643/1‑140 (MQ=255)
                                                                                         ggTTAGGCCGGCCGCTTTGATTTCAATATGATGCGGGTTTTGCATATATTTACGGCTCAATTTATCAATATCCTCCGGCAGCGTTGCCGAAAACAAAATCCTCGTGAGCTCTGTCGGCCGATTCTTTATAATCGCCTCTa                                                    >  2:3932346/1‑140 (MQ=255)
                                                                                          gTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTAc                                                   <  1:1218015/140‑1 (MQ=255)
                                                                                             aGGCCGGCCGCTTTCATTTCAATATGCTGCGGGTTTTGAATATATTGACGGCTCAATTTCTCAAAATCCTCCGGCCGCGTTGCCGGAAACAACATCGGCGTGCGCGCTGTGGGCAGGTCGTTGCTAATCGCCTCTACGTg                                                >  2:6793004/1‑140 (MQ=255)
                                                                                               gccggccgCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCt                                              <  1:6611026/140‑1 (MQ=255)
                                                                                               gccggccgCTTTGATTTCAAGATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCt                                              <  1:4680206/140‑1 (MQ=255)
                                                                                                   gccgCTTTGATTTCAATATTCTGCGGGTTTTTCATATATTTACGGCTCAAATTATAAATATACTCCGGCAGCGGTGCCGAAAAAAAAAACGTCGTGCGCTCTGTCGGCAGATTCTTTATAATAGCCTCTACTTTCTCGAt                                          >  1:2370860/1‑140 (MQ=255)
                                                                                                   gccgCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGAt                                          <  1:1430273/140‑1 (MQ=255)
                                                                                                   gccgCTTTGATTTCAAAATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGAt                                          >  2:5097273/1‑140 (MQ=255)
                                                                                                     cgCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGa                                        <  1:6073430/140‑1 (MQ=255)
                                                                                                     cgCTTTGATTTCAATATGCTGAGGGTTTGGCATATATTGAGGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACGTGCTCGATGa                                        <  2:3360233/140‑1 (MQ=255)
                                                                                                           gATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCa                                  <  2:5592952/140‑1 (MQ=255)
                                                                                                                 aaTATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCa                            >  1:5064597/1‑140 (MQ=255)
                                                                                                                  atatGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAAt                                                            <  1:2497963/107‑1 (MQ=255)
                                                                                                                  atatGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAAt                                                            >  2:6686881/1‑107 (MQ=255)
                                                                                                                     tgctgcGGGTTTTGCATATATTGACGGCTCAATTTCTCAAGATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCAt                        <  2:1423317/140‑1 (MQ=255)
                                                                                                                      gctgcGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCAtc                       >  1:929411/1‑140 (MQ=255)
                                                                                                                        tgcGGGTTTTCCATATATTGACGGCTCAATTTCTCTATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCAtctc                     <  2:7586203/140‑1 (MQ=255)
                                                                                                                          cGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGc                                                         >  2:1113396/1‑102 (MQ=255)
                                                                                                                          cGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCAt                   >  1:4361605/1‑140 (MQ=255)
                                                                                                                          cGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCAt                   >  1:532954/1‑140 (MQ=255)
                                                                                                                          cGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCAt                   >  1:2710043/1‑140 (MQ=255)
                                                                                                                             gTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGaaaa                                                                                                   <  2:6085121/57‑1 (MQ=255)
                                                                                                                             gTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGaaaa                                                                                                   <  2:4228204/57‑1 (MQ=255)
                                                                                                                               tttGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCt              >  1:6009201/1‑140 (MQ=255)
                                                                                                                                 tGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTtc            >  2:4756759/1‑140 (MQ=255)
                                                                                                                                 tGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTtc            >  1:7496345/1‑140 (MQ=255)
                                                                                                                                 tGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTtc            >  1:1114804/1‑140 (MQ=255)
                                                                                                                                 tGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTtc            >  1:7686762/1‑140 (MQ=255)
                                                                                                                                 tGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTtc            >  1:1254444/1‑140 (MQ=255)
                                                                                                                                       tatTGACGGCTCAGCTTCTCGATATCCTGCGGAAGCGTCGCTGAAAACAGCATCGTCGTACGCTCAGTCGGCAGATGCTTGATAATCGCCTCCACCTGCTCAATGAAGCCCATATTCAGCATCTCATCCGCTTCATCAAt      <  1:4794097/140‑1 (MQ=255)
                                                                                                                                       tatTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAAt      >  2:2483371/1‑140 (MQ=255)
                                                                                                                                       tatTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAAt      <  2:7686709/140‑1 (MQ=255)
                                                                                                                                       tatTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAAt      <  1:2931200/140‑1 (MQ=255)
                                                                                                                                       tatTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAAt      >  1:5800219/1‑140 (MQ=255)
                                                                                                                                       tatTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAAt      >  2:3766776/1‑140 (MQ=255)
                                                                                                                                       tatTGACGGCTCAATTTCTCAAGATCCTCCGGCAGCGGTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAAt      <  1:5949519/140‑1 (MQ=255)
                                                                                                                                         ttGACGGCTCACTGTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGa    <  2:7424127/140‑1 (MQ=255)
                                                                                                                                         ttGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATCTTCAGCATCTCATCGGCTTCATCAATGa    <  1:4263816/140‑1 (MQ=255)
                                                                                                                                         ttGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGa    <  1:3314097/140‑1 (MQ=255)
                                                                                                                                         ttGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGa    <  1:145819/140‑1 (MQ=255)
                                                                                                                                         ttGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCGCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGa    <  1:1001629/140‑1 (MQ=255)
                                                                                                                                          tGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGAc   <  1:4942132/140‑1 (MQ=255)
                                                                                                                                          tGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGAc   <  1:7154764/140‑1 (MQ=255)
                                                                                                                                           gACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGAcc  >  1:22925/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TCGGGATTCTCCGTCATCAGCACATCTTTCAGCAAAGAAAACTTATTCTCTTCTCTTACTTGAATCACCGCATGTTCAATATTCTTTGTGGTTAGGCCGGCCGCTTTGATTTCAATATGCTGCGGGTTTTGCATATATTGACGGCTCAATTTCTCAATATCCTCCGGCAGCGTTGCCGAAAACAACATCGTCGTGCGCTCTGTCGGCAGATGCTTGATAATCGCCTCTACTTGCTCGATGAAGCCCATATTCAGCATCTCATCGGCTTCATCAATGACC  >  NZ_CP034943/3846485‑3846763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: