Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,851,254 G→C 6.2% L41V (CTT→GTT)  EO946_RS19820 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,851,2540GC 6.2% 1123.7 / 10.4 511L41V (CTT→GTT) EO946_RS19820hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (343/122);  new base C (11/30);  total (359/152)
Fisher's exact test for biased strand distribution p-value = 3.19e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 541 total aligned reads displayed.

CTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAG  >  NZ_CP034943/3851115‑3851393
                                                                                                                                            |                                                                                                                                           
ctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAg                                                                                                                                             >  1:4212563/1‑140 (MQ=255)
 tctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAACCATAACCTTCAAAGTACTCGGACATGTCTTCTATAAATGAACCAGTCGTTAATACCTGTATCATGATTAGATCGTaagcaagc                                                                                                                                            >  2:381885/1‑140 (MQ=255)
   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                                          >  1:2735548/1‑140 (MQ=255)
   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                                          >  1:6725229/1‑140 (MQ=255)
   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                                          >  2:7265532/1‑140 (MQ=255)
   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                                          >  2:2810302/1‑140 (MQ=255)
    gTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCt                                                                                                                                         >  1:4703549/1‑140 (MQ=255)
        tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATCGTATGCAAGCCCTTccc                                                                                                                                     >  2:1443403/1‑140 (MQ=255)
           ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                                                                  <  1:7478027/140‑1 (MQ=255)
           ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                                                                  >  2:649894/1‑140 (MQ=255)
             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCAAGCCCTTCCCaaaaa                                                                                                                                >  1:5404821/1‑140 (MQ=255)
             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                >  1:7396197/1‑140 (MQ=255)
             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                >  1:164589/1‑140 (MQ=255)
             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                >  2:4141081/1‑140 (MQ=255)
             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                >  1:3325534/1‑140 (MQ=255)
             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                >  1:3480156/1‑140 (MQ=255)
             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                                                                  <  2:2962456/138‑1 (MQ=255)
             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATGCAAGCCCTTCCCaaaaa                                                                                                                                >  2:7694459/1‑140 (MQ=255)
             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATGCAAGCCCTTCCCaaaaa                                                                                                                                >  2:2446818/1‑140 (MQ=255)
             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCGGTATCATGATTAGATCGTATGCAAGCCCGTCCCaaaaa                                                                                                                                >  1:2925108/1‑140 (MQ=255)
             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCAGTATCATGATTCGATCGTATTCAACCCCTTACCaaaaa                                                                                                                                >  2:4736869/1‑140 (MQ=255)
             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACACGGACAGGTCTTCTATAAATGAACCATTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                >  2:5970000/1‑140 (MQ=255)
               tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACg                                                                                                                              >  2:5780211/1‑140 (MQ=255)
               tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGGTCTTACCTGTATCATGATTCGATCGTATGCAACCCCTTCCCCAAAACg                                                                                                                              >  1:286124/1‑140 (MQ=255)
                            ctctTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGActct                                                                                                                 >  2:5176350/1‑140 (MQ=255)
                               ttttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGGTATTACCTGTATCATTATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTATGct                                                                                                              >  1:4403613/1‑140 (MQ=255)
                                  tCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAAAGACTCTGcttct                                                                                                           >  1:4120376/1‑140 (MQ=255)
                                  tCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAATGATTAGATCGTATGCAAGCACTGACCAAAAAACATAAAACTAATCAGcttct                                                                                                           >  1:522242/1‑140 (MQ=255)
                                  tCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCAGTATCATGATTCGATAGTATTCAAGCCCTTCCCAAAAACCATAAAACGAATCTGcttat                                                                                                           >  1:3974645/1‑138 (MQ=255)
                                           ttATCACATTTTAAGAATAACATTCAAAGTACTCGGACTGTTATTATATAAATCAACCCGTGGTTATTACCTTTATCTTTATTCGATCGTATGCAACCCATACCAAAAAACGATAACACGACTCTGCTTCTTCAAGTTCg                                                                                                  <  2:6137307/140‑1 (MQ=255)
                                           ttATAACTTTTTAACAATAACCTTAAATGTACTCGCACTTGTATTATATAAATCAACCATTCTTTTTTACCTGTATATTGATTCGATCGTATGCAAGCCCTTCCAAAAACCGAAAACACGACTCTGCTTCTTCAAGTTCg                                                                                                  <  2:3058905/140‑1 (MQ=255)
                                                  cTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACa                                                                                           >  2:4911302/1‑140 (MQ=255)
                                                  cTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTACCAAAAACGATAAAACGACTCTGCTTATTCAAGTTCGCAAGACa                                                                                           >  2:6708837/1‑140 (MQ=255)
                                                   ttGTGAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAACTTCGCAATACaa                                                                                          >  1:7223613/1‑139 (MQ=255)
                                                   ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCAAGCCCTTCCCAAAAACGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACAt                                                                                          >  1:3072715/1‑140 (MQ=255)
                                                   ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTAGCAAGACAt                                                                                          >  2:1115046/1‑140 (MQ=255)
                                                   ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGAAAGCCCTTCCCAAAAACGATAAAACTAATCTGCTTCTTCAAGTTCGCAAGCCAt                                                                                          >  2:4457625/1‑140 (MQ=255)
                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTTTGCAAGCCCGTCCCAAAAACGATAAAACGCCTCTGCTTCTTCAAGTTCGCAAGACATAACACCTtg                                                                                 >  1:4505523/1‑140 (MQ=255)
                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATCAAACGACTCTGCTTCTTCAAGTTCGCAACACATAACAACTtg                                                                                 >  2:6818086/1‑140 (MQ=255)
                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACAtg                                                                                 >  1:3675802/1‑140 (MQ=255)
                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                 >  1:2013688/1‑140 (MQ=255)
                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATGCAAGCCCTTCCCAAAAACGATAAAACGACACTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                 >  2:357107/1‑140 (MQ=255)
                                                               ccTTCAATGTACTGGGACTGTTTTTCTATAAATCACCCAGTCTTTATTACATTTATATTGTTTGGATCTTATCAAAGCCCTACCAAAAACCCAAACCACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  1:6440954/140‑1 (MQ=255)
                                                               ccTTCAATGTACTCGGACAGGTTTTCTATAAATGAACCAGTCTTTTTTACCTGTATATTTTTTCGATCGTATGCAAGCCCTACCAAAAAACCAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:7493798/140‑1 (MQ=255)
                                                               ccTTCAAAGTACTCGCACCGGTCTTCTATAAATGAACCAGTCTTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAACCCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:438665/140‑1 (MQ=255)
                                                               ccTTCAAAGTACCCGCACTGTTATTATATAATTCACCCCGTCTTTTTTCCTGTTATCATGATTCTATCGTAGCCACGCCCTCCCAAAAAACCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:1023147/140‑1 (MQ=255)
                                                               ccTTAAAAGTACTCGTACCGTATTTATATAAATCACCCTGTGTTTTTTACTTGTATCTTTATTCTATCTTATGCACCCCCTCCCCAAAAACGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:5500620/140‑1 (MQ=255)
                                                               ccTACAAAGTACTCGTACAGTTTTTATATAAATCAACCAGTCTTTATTCCCTGTATCATTATGCGATCGTATCCAACCCCTTCCCAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:16965/140‑1 (MQ=255)
                                                               actccAACGTACTCGGACCTGTCTTCTATAAATCACCCCGTCTTTTTTACCTCTATCATGATCCGTGCGTATCCACGCCCTCCCCAAAAACAATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:2013688/136‑1 (MQ=255)
                                                                    aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTAGTCAAGTTCGCAAGACATAACAACTTGATGATGCt                                                                         >  2:662127/1‑140 (MQ=255)
                                                                     aattACTCGCACTGTACTTCTATAAATCACCCACTCTTTATTACCTGTATCATTATTCGATCGTATGCACGCCCTCCCCAAAACCGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCtt                                                                        <  1:7685695/137‑1 (MQ=255)
                                                                          cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCACGACATAACACAGTGATGAGGCTTAagag                                                                   >  2:4351978/1‑140 (MQ=255)
                                                                          cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGTCATAACAACTTGATGAGGCTTAagag                                                                   >  1:5520438/1‑140 (MQ=255)
                                                                          cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAAATTGATGAGGCTTAagag                                                                   >  1:1143379/1‑140 (MQ=255)
                                                                          cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACGCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                                   >  1:4968715/1‑140 (MQ=255)
                                                                                  ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTTATGAGGCTTAAGAGCGATCATg                                                           >  1:6076869/1‑140 (MQ=255)
                                                                                      ttctATAAATGAACCAGTCTTTATTACCTGTATCATGATTCTATCTTATGCAAGCCCTTCCAAAAAACCAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTa                                                       <  2:1274001/140‑1 (MQ=255)
                                                                                      ttctATAAATCAACCATTCTTTTTTACGTTTATATTGATTCGATGTTATGCAAGCCCTCCCAAAAAACGAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTa                                                       <  2:324824/140‑1 (MQ=255)
                                                                                       tctATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                                                      >  1:2113065/1‑140 (MQ=255)
                                                                                         tataAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGAAACCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACAAGATCAGGCTTAAGAGCGATCATGTCTaaaa                                                    >  2:5990025/1‑139 (MQ=255)
                                                                                          ataCATGAACCCGTGGTTATTACCTGTATCTTTATTCTTTGTTCTCCACCCCCTTCCCAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCAGGTCTAAAtt                                                   <  2:870571/140‑1 (MQ=255)
                                                                                            aaaTCAACCAGTCGTTATTACCTTTATCTTTATTCGCTCGTATGCAAGCCCTTCCCAAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACACCTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGc                                                 <  2:3924113/140‑1 (MQ=255)
                                                                                              aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTAAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCg                                               >  1:623784/1‑140 (MQ=255)
                                                                                              aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCg                                               >  2:2583745/1‑140 (MQ=255)
                                                                                              aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCg                                               >  1:5794743/1‑140 (MQ=255)
                                                                                                 aaCCAGTTTTTTTTACCTTTATAATGATTCGATCGTATCCACGCCCGCCCCAAAAACCATACCACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                            <  1:101234/140‑1 (MQ=255)
                                                                                                 aaCCAGTGTTTTTTACGTTTATCATGATTCGATCTTATGCAAGCCCTGCCAAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                            <  2:5327741/140‑1 (MQ=255)
                                                                                                 aaCCAGTCTTTATTACCTTTATAATGATTCGATCTTATGCAACCCCTTCCCAAAACAGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                            <  2:2526467/140‑1 (MQ=255)
                                                                                                 aaCCAGTCTTTATTACCTGTATCTTGATTCGATCGTCTGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                            <  2:719071/140‑1 (MQ=255)
                                                                                                 aaCCAGTCGTTATTCCCTTTATCATGATTCGATCGTATGCAAGCCCTTCCCAAACACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                            <  2:4233269/140‑1 (MQ=255)
                                                                                                  aCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGGTTCTTCAAGTTCGCAAGTCATAACAACTTGATGATGCTTAAGAGCGATCATGTCTAAATTGCCGATaa                                           >  1:1563447/1‑139 (MQ=255)
                                                                                                  aCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATaa                                           >  2:6967309/1‑139 (MQ=255)
                                                                                                  aCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTAAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGATCGATCATGTCTAAATTGCCGATAc                                           >  1:2260146/1‑140 (MQ=255)
                                                                                                  aCCAGTCGTTATTACCTGTATCATGATTAGATCGTATGCAACCCCTTCCCAAAAACGATAAAACCACTCAGCTTCTTCAAGTTAGCAAGACATAACAACTTTATGAGGCTTAATAGCGATCATGTCTAAATTGCCGATaa                                           >  1:7031745/1‑139 (MQ=255)
                                                                                                   ccAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                                          >  2:6403069/1‑140 (MQ=255)
                                                                                                   ccAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                                          >  2:5661899/1‑140 (MQ=255)
                                                                                                            attaCCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGACCGATCATGTCTAAATTGCCGATACTGCTtatatc                                 >  2:332010/1‑138 (MQ=255)
                                                                                                            attaCCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTAGCAATACATAACAACTTTATTAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGc                                 >  2:1855902/1‑140 (MQ=255)
                                                                                                            attaCCTGTATAATGATTCGATCGTATGCAACCCCTTCCCAAAAACGATAAAACGACACTGCTTCTTAAAGTTCGCAAGAAAAAACAACTTGATGAGGCGTAAGAGCGATCATGTCTAAATTGCCGATACTGCTtatatc                                 >  1:683213/1‑138 (MQ=255)
                                                                                                                     tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt                         >  2:1531464/1‑140 (MQ=255)
                                                                                                                      aTCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTc                        >  1:3258831/1‑140 (MQ=255)
                                                                                                                          tGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATa                    >  2:6236573/1‑140 (MQ=255)
                                                                                                                               ggagcGTATGAAAGCCGTTCCCCAACCCTATAAAACGACTCTGCTTCTTCATGTTCTCAAGACATAACAACTTGTTGAGTCTTATGAGCGATCATGTCTAAATTGCCTTTACTGATTATAGCGGTTATTTCCATAAAATc               <  2:5358258/136‑1 (MQ=255)
                                                                                                                               cGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATc               >  1:1715938/1‑140 (MQ=255)
                                                                                                                               cGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTACTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATc               >  1:5121554/1‑140 (MQ=255)
                                                                                                                                 aTCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCtt             >  2:4513388/1‑140 (MQ=255)
                                                                                                                                 aTCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATAtt             >  2:5868171/1‑140 (MQ=255)
                                                                                                                                    ttATGCAAGCCCTCCCAAAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATATCAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTccc          <  2:1640051/139‑1 (MQ=255)
                                                                                                                                       tGCACGCCCTCCCAAAAAACTAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt       <  1:6935480/140‑1 (MQ=255)
                                                                                                                                       tGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt       >  1:359390/1‑140 (MQ=255)
                                                                                                                                       tGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCGTATAGCGGTTATTTCCATAAAAGCTTCCCGtt       >  2:7604845/1‑140 (MQ=255)
                                                                                                                                         cAAGCCCTTCCAAAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTAt     <  2:7395338/140‑1 (MQ=255)
                                                                                                                                         cAACCCCTCCCCAAACACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTAt     <  1:6319936/140‑1 (MQ=255)
                                                                                                                                            gCCCTTCCCAAAACCGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg  <  2:5071978/140‑1 (MQ=255)
                                                                                                                                            gCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCACGACATAACAACTTGATGAGGCTTAAGAGCTATCATGTCTAAAGTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg  >  1:2636814/1‑140 (MQ=255)
                                                                                                                                            gCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg  >  2:6925384/1‑140 (MQ=255)
                                                                                                                                            gCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg  >  1:3789134/1‑140 (MQ=255)
                                                                                                                                            gCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg  >  2:3417659/1‑140 (MQ=255)
                                                                                                                                            gCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg  >  1:5324313/1‑140 (MQ=255)
                                                                                                                                            gCCCTTCACAAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg  <  2:2167200/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
CTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAG  >  NZ_CP034943/3851115‑3851393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: