Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 4,040,429 C→A 6.3% R197L (CGT→CTT)  mnmG ← tRNA uridine‑5‑carboxymethylaminomethyl(34) synthesis enzyme MnmG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349434,040,4290CA 6.3% 1978.0 / 28.0 759R197L (CGT→CTT) mnmGtRNA uridine‑5‑carboxymethylaminomethyl(34) synthesis enzyme MnmG
Reads supporting (aligned to +/- strand):  ref base C (263/416);  new base A (78/2);  total (341/418)
Fisher's exact test for biased strand distribution p-value = 4.03e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 814 total aligned reads displayed.

CCAGCAAGGCAGCTGATCTGTAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCCTCTTAAATATGTCCCTGTCGTCATGACAACTGC  >  NZ_CP034943/4040290‑4040568
                                                                                                                                           |                                                                                                                                            
ccagcAAGGCAGCTGATCTGTAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAaac                                                                                                                                              >  1:1816335/1‑140 (MQ=255)
ccagcAAGGCAGCTGATCTGTAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGGTTTGCTGTAATCAAGCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAaac                                                                                                                                              >  2:3695665/1‑140 (MQ=255)
    cAAGGCAGCTGATCTGTAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAaacgaac                                                                                                                                          <  2:1237642/140‑1 (MQ=255)
    cAAGGCAGCTGATCTGTAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAaacgaac                                                                                                                                          <  1:2335131/140‑1 (MQ=255)
    cAAGGCAGCGGATCTGTAAGATATTCCACTGGTTCATATGAGAAAGCGCGACGCACATCGTCACCAGGCTGACTTTCCGTTTTGCTGTAATCAATCGCGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAaacgaac                                                                                                                                          <  2:5865015/140‑1 (MQ=255)
     caGGCATCTGATCTGTAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAaacgaacg                                                                                                                                         <  2:1991042/139‑1 (MQ=255)
     aaGGCAGCTGATCTGTAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAaacgaacg                                                                                                                                         <  1:3207677/140‑1 (MQ=255)
          agctgagctgTAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAAGTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTTACGCGAGGCGGCGTACCTGTTTTAAAACGAACGCGGtg                                                                                                                                    >  1:4075263/1‑139 (MQ=255)
                tCTGTAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGcc                                                                                                                             <  2:5373779/140‑1 (MQ=255)
                   gTAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAg                                                                                                                          <  1:6292195/140‑1 (MQ=255)
                         tatTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTTAAGCGAGGCGGCGGACCTGTTTTAAAACCAACCAGGTCAAAGCCCAGAtactc                                                                                                                    >  2:4339001/1‑140 (MQ=255)
                           ttCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTTAAGCGAGGCGGCGTACCTGTTTTAAAAAGAACGAGGTCAAATCCCAGTTCCTCCa                                                                                                                  >  2:6662961/1‑140 (MQ=255)
                            tCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAAAGAGGTCAAAGCCAAGTTCCTCCaa                                                                                                                 >  1:5748437/1‑140 (MQ=255)
                            tCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAAAGAACGAGGTCAAAGCCCAGTTGCTCCaa                                                                                                                 >  1:980140/1‑140 (MQ=255)
                            tCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCGTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCACTTCCTCCaa                                                                                                                 >  1:867905/1‑140 (MQ=255)
                              cACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAAt                                                                                                               >  2:4068380/1‑140 (MQ=255)
                              cACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAAt                                                                                                               >  1:7315248/1‑140 (MQ=255)
                              cACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCACCAAAt                                                                                                               >  2:467681/1‑140 (MQ=255)
                              cACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAAAGAACGAGGTCAAAGACCCGTTACTACAAAt                                                                                                               >  2:4414144/1‑140 (MQ=255)
                              cACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTAACAGTTTTAAAAAGAACGATGTCAAAGACCAGTTACTCCAAAt                                                                                                               >  2:2439559/1‑140 (MQ=255)
                              cACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGGACCTGGTTTAAAAAGAACGAGGTCAAAGCCCAGTTGCTCCAAAt                                                                                                               >  1:364184/1‑140 (MQ=255)
                                  gTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGAAGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTGCTCCAAATGCTc                                                                                                           >  1:4688802/1‑140 (MQ=255)
                                   tttCATATGAGAAAGCGCTAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCAGTTTTAAAACGAAACAGGTCAAAGCACACTTACTCCAAATGCTCa                                                                                                          >  2:7170817/1‑140 (MQ=255)
                                   tttCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGAGGCGTGGCGGCGTACCTGTTTTAAAAAGAACGAGGTGAAAGACCCGTTGCTCCAAATGGTCa                                                                                                          >  1:1001481/1‑140 (MQ=255)
                                   tttCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCa                                                                                                          <  2:5664048/140‑1 (MQ=255)
                                   tttCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCa                                                                                                          >  2:1751622/1‑140 (MQ=255)
                                   tttCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACATGTTTTAAAAAGAAAGAGGTAAAACGCCCAGTCCGCCAAAAGCTCa                                                                                                          >  2:6525628/1‑140 (MQ=255)
                                   tttCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAAGTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGAACCGAGGCGGCGGACCTGGTTTATAAACAAAGAGGTCAAATCCCAGTTACTCCAAATGATCa                                                                                                          >  1:5003179/1‑140 (MQ=255)
                                        tatGAGAAAGCGCGAGGCACATCGTCACCAGGCTGTCTTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGATGTCAAATCCCAGTTCCTCCCAATGCTGACAAAg                                                                                                     >  2:7506204/1‑140 (MQ=255)
                                        tatGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAg                                                                                                     <  2:3863869/140‑1 (MQ=255)
                                          tGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCt                                                                                                   <  2:6078083/140‑1 (MQ=255)
                                          tGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGGAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCt                                                                                                   <  2:2844869/140‑1 (MQ=255)
                                          tGAGAAAGCGCGAGGCACATAGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCt                                                                                                   <  2:1492991/140‑1 (MQ=255)
                                             gaAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTTACGCGAGGCGGCGTACCAGTTTTAAAAAGAACGAGGTCAAAGCCCATTTCCTCCAAATGCTCAGAAAGCTTaa                                                                                                >  1:5634421/1‑140 (MQ=255)
                                             gaAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACCAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTaa                                                                                                >  2:4957627/1‑140 (MQ=255)
                                                  cgcgAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGaa                                                                                           >  2:4199370/1‑140 (MQ=255)
                                                  cgcgAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCGTTTGACGCGAGGCGGCGTACCTGTTTTAAAAAGAACGATGTCAAAGCCCAGTTGCTCCAAATTCTCCGAAAGCTTAATATaa                                                                                           >  1:3336588/1‑140 (MQ=255)
                                                  cgcgAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCAGTTGACGCGCGGCGGCGTACCTGTTTTAAAACGAACGAGGGCAAAGCCCAGTTCCTCCAAATGCGCCGAAAGCTGAATAGaa                                                                                           >  2:7305911/1‑140 (MQ=255)
                                                       ggCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGACGgctg                                                                                      <  2:6300717/140‑1 (MQ=255)
                                                        gCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGgctgc                                                                                     <  2:2556718/140‑1 (MQ=255)
                                                        gCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGAAGCGAGGCGGCGTACCGGTTTTAAAACGAACGAGGTCAAAGCCCACTTACTCCAAATTCTAAGAAAGCTTAATAGAAGgctgc                                                                                     >  2:3071255/1‑140 (MQ=255)
                                                        gCACATCGTCACCAGGCTGAAGTTCCGTTTTGCTGTAATCAATCGAGTCGCATTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGgctgc                                                                                     <  1:3584851/140‑1 (MQ=255)
                                                          acaTCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGctgctg                                                                                   <  2:84762/140‑1 (MQ=255)
                                                                tCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTAtt                                                                             <  2:7037094/140‑1 (MQ=255)
                                                                 cACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGTCGCGAGGCGGCGGACCGGTTTTAAAAAGAAAGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATATGAGGCTGCTGGTTATTc                                                                            >  2:3329811/1‑140 (MQ=255)
                                                                    cAGGCTGAATTTCCGTTTTGCTGTAAGCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGa                                                                         <  1:6672757/140‑1 (MQ=255)
                                                                     aGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGAc                                                                        <  1:2495353/140‑1 (MQ=255)
                                                                      ggCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAAAGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGAcc                                                                       >  2:6714263/1‑140 (MQ=255)
                                                                        gtGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGc                                                                     <  2:488230/139‑1 (MQ=255)
                                                                        cccactgtCCGATGTGCTGTAACCAAGCGAGTCGCTATTGCCGCCAGGCGGCGTACCTGAGATAAAACGAACGCGGTCAAAGCCCAGTTCCTCCAACTGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGc                                                                     <  1:4184471/133‑1 (MQ=255)
                                                                        cTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGCCGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCACATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCgg                                                                     <  1:1336088/140‑2 (MQ=255)
                                                                        cTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCTGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTGCAAATGCTCAGAAAGCTTAATAGAAGGCTGATGGTTATTCGGACCGc                                                                     <  2:6002045/140‑1 (MQ=255)
                                                                        cTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGACGGCTGCTGGTTATTCGGACCGc                                                                     <  1:1747219/140‑1 (MQ=255)
                                                                        cTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGc                                                                     <  1:4613380/140‑1 (MQ=255)
                                                                        cTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGc                                                                     <  1:741534/140‑1 (MQ=255)
                                                                        cTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGc                                                                     <  1:2796185/140‑1 (MQ=255)
                                                                        cTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGc                                                                     <  1:3467430/140‑1 (MQ=255)
                                                                        cTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGc                                                                     <  1:6525666/140‑1 (MQ=255)
                                                                        cTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGc                                                                     <  1:2683120/140‑1 (MQ=255)
                                                                        cTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGc                                                                     <  2:2720054/140‑1 (MQ=255)
                                                                        cTGAATGTCCGTTTTTCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGc                                                                     <  1:7668250/140‑1 (MQ=255)
                                                                        cTGAATGTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGCTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGc                                                                     <  2:1131858/140‑1 (MQ=255)
                                                                         tGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCCCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCt                                                                    <  1:3058308/140‑1 (MQ=255)
                                                                          gAATGTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCtt                                                                   <  2:1816335/140‑1 (MQ=255)
                                                                            aTTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCAGTTTTAAAACCAACGAGGTCAAAGCCCAGTTACTCCAAATGATCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGa                                                                 >  2:2201336/1‑140 (MQ=255)
                                                                             tttCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAg                                                                <  2:3437202/140‑1 (MQ=255)
                                                                                 cGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAg                                                            <  1:7036440/140‑1 (MQ=255)
                                                                                           tAATCAATCGAGTCGCTTTTGTAGCGAGGCGGCGTACCGGTTTTAAAACGAACCACGTCAAAACCCACTTACTCCAAATGCTCACAAACCTTAATAAAAGGCTGCTGGTTATTAGGACCGCTTGAGTAAAACAGATAAcc                                                  >  1:3724822/1‑140 (MQ=255)
                                                                                           tAATCAATCGAGTCGCTTTTGGCGCGGGGCGGCGTACCTGGTTTAAAACGAACGCGGTGAAAGCCCCGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCGGCTGGTTATTAGGCCCCCTTGATTAACAAAGATCAcc                                                  >  2:3207105/1‑140 (MQ=255)
                                                                                           tAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCTGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCAcc                                                  <  2:7604664/140‑1 (MQ=255)
                                                                                            aatcaatcGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCa                                                 <  2:1563186/140‑1 (MQ=255)
                                                                                                     aGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCCAATGCTCAGAAAGCTTAATATAAGGCTGCTGGTTATTAGGACCGCTTGTGTAAGAAAGATCACCAAGAATGAtt                                        >  2:695218/1‑140 (MQ=255)
                                                                                                      gTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTc                                       <  2:5757492/140‑1 (MQ=255)
                                                                                                       tCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCg                                      <  1:4510283/140‑1 (MQ=255)
                                                                                                        cGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGt                                     <  1:1986170/140‑1 (MQ=255)
                                                                                                        cGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGt                                     <  1:793361/140‑1 (MQ=255)
                                                                                                         gCTTTTGACGCGAGGCGGCGTCCCGGATTGAAACCGAACGAGGTCCCAGCCCACTTCCACCAACTGCTCACAAAGCAGAATACAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAACAATGATTCGTc                                    <  2:2319139/140‑1 (MQ=255)
                                                                                                         gCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATCGAAGGCTGCTGGTTATTCGGACCGCTGGAGTAAGACAGATCACCAAGAATGATTCGTc                                    <  2:4594769/140‑1 (MQ=255)
                                                                                                         gCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTc                                    <  2:5287796/140‑1 (MQ=255)
                                                                                                         gCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTc                                    <  2:5582031/140‑1 (MQ=255)
                                                                                                         gCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTc                                    <  1:142960/140‑1 (MQ=255)
                                                                                                         gCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTc                                    <  1:1649153/140‑1 (MQ=255)
                                                                                                         gCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTc                                    <  2:2972622/140‑1 (MQ=255)
                                                                                                          cTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAACGGCTCAGAAAGCTTAATAGCAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTcc                                   <  1:5886883/140‑1 (MQ=255)
                                                                                                          cTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTcc                                   <  1:5173303/140‑1 (MQ=255)
                                                                                                             ttGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCctct                                <  2:7745211/140‑1 (MQ=255)
                                                                                                                     aGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCCTCTTAAATATg                        >  1:3840088/1‑140 (MQ=255)
                                                                                                                     aGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCCTCTTAAATATg                        >  2:5492284/1‑140 (MQ=255)
                                                                                                                     aGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCCTCTTAAATATg                        >  1:7579778/1‑140 (MQ=255)
                                                                                                                     aGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTGGAGTACGCCCGATCACCAAGAATGAGTCGTGCTCTGCAATATg                        >  2:6423072/1‑140 (MQ=255)
                                                                                                                          gcgTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCCTCTTAAATATGTCCCt                   <  1:6167173/140‑1 (MQ=255)
                                                                                                                           cgTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCCTCTTAAATATGTCCCTg                  <  1:1189731/140‑1 (MQ=255)
                                                                                                                             tACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCCTCTTAAATATGTCCCTgtc                >  2:1336714/1‑140 (MQ=255)
                                                                                                                                cTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCCTCTTAAATATGTCCCTgtcgtc             <  1:5413498/140‑1 (MQ=255)
                                                                                                                                cTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCCTCTTAAATATGTCCCTgtcgtc             <  1:5292905/140‑1 (MQ=255)
                                                                                                                                cTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCCTCTTAAATATGTCCCTgtcgtc             <  1:4847486/140‑1 (MQ=255)
                                                                                                                                     ttAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCCTCTTAAATATATCCCTGTCGTCATGAc        >  1:6843386/1‑140 (MQ=255)
                                                                                                                                        aaaCGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCCTCTTAAATATGTCCCTGTCGTCATGACAAc     >  1:5544174/1‑140 (MQ=255)
                                                                                                                                           cgaccgaGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCCTCTTAAATATGTCCCTGTCGTCATGACAACTGc  <  2:5128853/140‑1 (MQ=255)
                                                                                                                                           cgaacgaGGTCAAAGCCCAGTTCCTCCACATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACATATCACCAAGAATGATTCGTCCTCTTAAATATGTCCCTGTCGTCATGACAACTGc  <  2:6229066/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
CCAGCAAGGCAGCTGATCTGTAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCCTCTTAAATATGTCCCTGTCGTCATGACAACTGC  >  NZ_CP034943/4040290‑4040568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: