Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 316,262 C→A 7.0% Q28K (CAA→AAA)  opuAA → glycine/proline betaine ABC transporter ATP‑binding protein OpuAA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943316,2620CA 7.0% 1679.2 / 18.9 674Q28K (CAA→AAA) opuAAglycine/proline betaine ABC transporter ATP‑binding protein OpuAA
Reads supporting (aligned to +/- strand):  ref base C (392/204);  new base A (6/71);  total (398/276)
Fisher's exact test for biased strand distribution p-value = 1.16e-23
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 680 total aligned reads displayed.

TGTGGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAAC  >  NZ_CP034943/316124‑316401
                                                                                                                                          |                                                                                                                                            
    gAATGTTACATCAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGTAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAAtt                                                                                                                                         >  1:4908884/1‑139 (MQ=255)
      aTGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCt                                                                                                                                       >  2:2908467/1‑140 (MQ=255)
          tACATAAGTGTTTCTGTTATAAGTATTGTATAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGcc                                                                                                                                   <  2:4528938/140‑1 (MQ=255)
          tACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGcc                                                                                                                                   >  1:5262838/1‑140 (MQ=255)
               aaGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAATTTCTTGCCAACgg                                                                                                                              >  2:5113369/1‑140 (MQ=255)
                   gtTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAg                                                                                                                         >  2:1850195/1‑140 (MQ=255)
                    ttACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGTGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGa                                                                                                                        >  1:979035/1‑140 (MQ=255)
                    ttACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATTCTTTCCAACGGAAAGa                                                                                                                        >  1:5502517/1‑140 (MQ=255)
                    ttACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGa                                                                                                                        >  2:1281525/1‑140 (MQ=255)
                    ttACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGa                                                                                                                        >  1:2426810/1‑140 (MQ=255)
                     tACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGAc                                                                                                                       >  2:3424274/1‑140 (MQ=255)
                      aCGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAACCAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACa                                                                                                                      >  1:3522091/1‑140 (MQ=255)
                      aCGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGa                                                                                                                        >  2:6580974/1‑138 (MQ=255)
                            ataAGTATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                                >  1:6628819/1‑140 (MQ=255)
                            ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATCAAAGTCGAGAAAGTATCTAAAATTTTTGGGAACCAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                                >  2:1694356/1‑140 (MQ=255)
                            ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCCGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                                >  2:6713115/1‑140 (MQ=255)
                            ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                                >  1:1337394/1‑140 (MQ=255)
                              caGGAGTGTTTAATATGGCGCGAAACCACTAGCTTACAAGACCACCCAATTACCATCCAAGTCGATAAAGCATCTAAAATTTTTGCGAACCAAACAAAGAAAGCAGTTCAAATGCTTGCCCACGCAAAGACAAAAAAaga                                                                                                              <  2:2381745/139‑1 (MQ=255)
                              aaGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAACCACACCATGACAGCAGTTCAAATGCGTGCCAACGGAAAGACAAAAAAcga                                                                                                              >  2:7096555/1‑140 (MQ=255)
                              aaGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAACTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGATCAAATGCTTGCCAACGGACAGACAAAAAAaga                                                                                                              >  2:1178006/1‑140 (MQ=255)
                                  aTTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATc                                                                                                          >  2:721750/1‑140 (MQ=255)
                                    tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAgaga                                                                                                            <  1:2743570/136‑1 (MQ=255)
                                    tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  1:6183430/1‑140 (MQ=255)
                                    tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  2:5241606/1‑140 (MQ=255)
                                    tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  1:678191/1‑140 (MQ=255)
                                    tGTTTAATATGGAGGGAAAATATCAGTGGAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  2:3167710/1‑140 (MQ=255)
                                      tttAATATTGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAACTCGAGAAAGAATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACTGAAAGACAAAAAAAGAGATACTGa                                                                                                      >  1:5135773/1‑140 (MQ=255)
                                      tttAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGa                                                                                                      >  1:5364498/1‑140 (MQ=255)
                                      tttAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGa                                                                                                      >  2:7713037/1‑140 (MQ=255)
                                      tttAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGa                                                                                                      >  1:7420012/1‑140 (MQ=255)
                                           tatGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCg                                                                                                 >  1:4463745/1‑140 (MQ=255)
                                                atgtaaaaTATGAGTGTAGATGAGAAAAAAAAAAATATAAAAATCGAGAAAGAATATAAAATTTTTGTGAAACAAACAAATAAAGAATTTAAAATTCTTTCAAAATGAAAGACAAAAAAAGAGATACTGAAAGCGAACgg                                                                                            >  2:3684115/5‑140 (MQ=255)
                                                aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCTAGAAAGTAGCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGAATGCCAACGGAAAGAAAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  1:3685631/1‑140 (MQ=255)
                                                aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAagag                                                                                                             <  2:2332401/123‑1 (MQ=255)
                                                aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  2:4010973/1‑140 (MQ=255)
                                                aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  1:5663037/1‑140 (MQ=255)
                                                aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  1:234399/1‑140 (MQ=255)
                                                aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  2:7300632/1‑140 (MQ=255)
                                                aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  1:1005972/1‑140 (MQ=255)
                                                          tGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGtt                                                                                  >  1:5742135/1‑140 (MQ=255)
                                                            aGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCACCCGGTgg                                                                                >  2:5872124/1‑140 (MQ=255)
                                                             gtgtAGAGCACAAACCAATTAACATAAACGTCCACAAAGTAACTAAAATTTTTGGTAAAAAAACAACGAAAGCAGTTAAAATGCTTCCCAACGCAAACACAAAAAAAGAGATCCTCAAAGCGACCGGATCAACCGTTggg                                                                               <  1:1660634/140‑1 (MQ=255)
                                                              tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                              >  2:6061867/1‑140 (MQ=255)
                                                              tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                              >  1:3119198/1‑140 (MQ=255)
                                                              tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                              >  1:2531168/1‑140 (MQ=255)
                                                              tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                              >  2:6963845/1‑140 (MQ=255)
                                                              tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGt                                                                             >  1:1185859/1‑140 (MQ=255)
                                                               gtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGGTGGGGt                                                                             >  2:4248628/1‑140 (MQ=255)
                                                                tAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTa                                                                            >  2:2634170/1‑140 (MQ=255)
                                                                  gATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGGTGGGGTaaa                                                                          >  2:7532785/1‑140 (MQ=255)
                                                                   aTGAGAAACCAATTAAGATAAAAGACGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGACAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTaact                                                                         >  2:5623517/1‑138 (MQ=255)
                                                                        aaaaaaaTTAATATAAAAGTCTATAAATTATATAAAATTTTTGGCAAACAAAAAAATAAAGCAGTTCAAATGTTTGCAAACTTAAATAAAAAAAAAGTGATCTTTAAATATACATTATAAACCGTTGGGGTAAATCaacc                                                                    <  2:2718998/135‑3 (MQ=255)
                                                                        aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAATCAGTTCAAATGATTGCCAACCGAAAGACCAAAAAAGAGATCCTCAAAGCGACCTGATCAACAGTTGGGGTAAATCAAGc                                                                    >  1:3425786/1‑140 (MQ=255)
                                                                        aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGACAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                    >  1:3291533/1‑140 (MQ=255)
                                                                        aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                    >  1:4040458/1‑140 (MQ=255)
                                                                        aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                    >  1:6957279/1‑140 (MQ=255)
                                                                        aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                    >  1:4595013/1‑140 (MQ=255)
                                                                        aaaCAAATTAATATAAAAGTCGATAAATTATCTAAAATTTTTGGGAAAAAAACAAATAAAGCATTTCAATGGCTTGCCAACGCAAACACAAAAAAAGACAGCGTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                    <  2:3071648/140‑1 (MQ=255)
                                                                          aCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAg                                                                  >  1:6344583/1‑140 (MQ=255)
                                                                          aCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGGTGGGGTAAATCAAGCAg                                                                  >  1:7024964/1‑140 (MQ=255)
                                                                          aCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGGTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAg                                                                  >  1:6110439/1‑140 (MQ=255)
                                                                              aTTAAGATAAAAGTCGAGAAAGTATATAAATTTTTTGGCAAACAAACAAAGAAAGCATTTAAAAGGCTTGCCAACGGAAACACAAAAAAAGAGAGCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAttt                                                              <  2:2004995/140‑1 (MQ=255)
                                                                                taataaaaacGTCTATAAATTATCTAAAATTTTTGGCAAAAAAAAAAACAAAGCAGTAAAATTGCTTGCCACCGCAAACAAAAAAAAAGAGATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                            <  1:3349406/134‑1 (MQ=255)
                                                                                tAATATAAAAGTCGATAAATTATCTAAAATTTTTGGCAAACAAACAAAGAAAGCAGTTCAAAGGCTTGCCACCGCAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                            <  2:1431284/140‑1 (MQ=255)
                                                                                tAAGATAAACGACGAGAAAGTATCTAAATTTTTGGGGAAACAAAAAAACAAAGCAGTAAAATGGCTTGCCACCGCAAACACAAAAAAACACATCCTTAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                            <  1:1877745/140‑1 (MQ=255)
                                                                                tAAGATAAAAGTCGATAACGTATATAAAATTTTTGGCAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAACAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                            <  2:4648557/140‑1 (MQ=255)
                                                                                tAAGAAAACAGTCGATAAAGTATATAAAATTTTTGGGAAACAAACAAACAAAGCAGTACAAATGCTTCCCAACGGAAAGACAAAAAAAGAGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                            <  2:2538911/140‑1 (MQ=255)
                                                                                  aGATAAACGTCGAGAAAGTATCTAAAATTTTTGGGAAAAAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAGGAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                          <  2:6226330/140‑1 (MQ=255)
                                                                                         aGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATatg                                                   <  2:4349144/140‑1 (MQ=255)
                                                                                               taacttatataAATTTTTTGGCAAAAAAAAAAATAACCCATTTCAAATGGTTGCCACCGCAAACAAAAAAAAACAGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTg                                             <  1:2614359/135‑1 (MQ=255)
                                                                                               gaAAGTATCTAAAATTTTTGGTAAACAAAAAAAGAAAGCAGTTAAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTg                                             <  2:5502517/140‑1 (MQ=255)
                                                                                               cactgTATCTAAATTTTTTGGCAAAAAAAAAAACAAAGCATTTAAAATGCTTGCCACCGCAAACAAAAAAAAAGAGACCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTg                                             <  1:4170423/136‑1 (MQ=255)
                                                                                               caacttATCTAAATTTTTTGGCAAAAAAACAAAGAAACCATTTAAAATGGTTGCCACCGCAAAAAAAAAAAAAGAGAGCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTg                                             <  1:875931/135‑1 (MQ=255)
                                                                                                  attATCTAAAATTTTTGGCAAAAAAACAAAGAAAGCAGTTAAAAGGCTTGCAAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTgaga                                          <  2:4129358/138‑1 (MQ=255)
                                                                                                     aTCTAAAATTTTTGGTAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGatat                                       <  2:379444/140‑1 (MQ=255)
                                                                                                     aTCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACCAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGatat                                       >  1:7259736/1‑140 (MQ=255)
                                                                                                     aTCTAAAATTTTTGGGAAAAAAAAAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCGGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGatat                                       <  2:218660/140‑1 (MQ=255)
                                                                                                       cTAAAATTTTTGGTAAAAAAACAAAGAAACCATTAAAAATCCTTGCCAACGCAAAGAAAAAAAAACAGATCCTGACAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATAttt                                     <  1:1490190/140‑1 (MQ=255)
                                                                                                         aaaaTTTTTGCGAAAAAAACAACCAACCCCCTGCAAAGCCTTCCCAACCCCAAGACAACAAAAGCCAGCCGGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGt                                   <  1:2063425/140‑1 (MQ=255)
                                                                                                              ttttGGCAAACAAACAAAGAAACCAGTTAAAATGCTTGCAAACCCAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGtcatca                              <  2:4733862/140‑1 (MQ=255)
                                                                                                               tttGGGAAACAAACAAACAACGCCTTTCAAATGCTTGCAACCGCAAAGACAAAAAAAGAGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                             <  1:4956153/140‑1 (MQ=255)
                                                                                                               tttGGGAAAAAAAAAAAGAAAGCAGTTCAACTGCTTGCCACCGCAAAGAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                             <  1:7624114/140‑1 (MQ=255)
                                                                                                               ttggcgAAAAAAACCAAGAACGCAGTAAAAATGCTTGCCACCGTAAATAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                             <  2:4947797/135‑1 (MQ=255)
                                                                                                               gttGGGAAACAAACAACGAAAGCAGTTAAAATGCTTGCCAACGGAACGACAAAAAAAGAGAGCCTGACAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                             <  2:5713613/139‑1 (MQ=255)
                                                                                                                ttGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATg                            >  1:6464998/1‑140 (MQ=255)
                                                                                                                ttGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATg                            >  2:2433454/1‑140 (MQ=255)
                                                                                                                 tGGTAAACACAAAAATAAAGCAGTTAAATTGCTTCCATAGGAAACTACAAAAAAATAGATCCTTAACGCGACCGGATCAACCGTTGGGTTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATgg                           <  2:6401146/140‑1 (MQ=255)
                                                                                                                  ggcaaaaaaacAAAGAAACCAGTTAAAATGGTTGCCAACGGAAACAAAAAAAAGACATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGt                         <  1:582704/137‑1 (MQ=255)
                                                                                                                   ggAAACAAAAAAAGAAACCAGTACAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGt                         <  2:3514273/140‑1 (MQ=255)
                                                                                                                            aaaataaagCTTTTCAATTGCTTCCCAACGCAAACAAAAAAAAATAGATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAggg                <  1:2967049/139‑1 (MQ=255)
                                                                                                                                  aagcttttCAAATGCTTCCCAACGTAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGt          <  1:4697944/134‑1 (MQ=255)
                                                                                                                                  aagaatttCAAATGCTTGCCAACGTAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGt          <  2:6337854/134‑1 (MQ=255)
                                                                                                                                   agCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTGGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTa         >  1:7215691/1‑140 (MQ=255)
                                                                                                                                   agCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTGGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGACATCATGGGTCTATCAGGGAGCGGTa         >  2:3329768/1‑140 (MQ=255)
                                                                                                                                     cAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTaaa       >  1:1797450/1‑140 (MQ=255)
                                                                                                                                      aGTTCAAATGCTTGCCAACGGAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATAGGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAAt      <  1:2247022/140‑1 (MQ=255)
                                                                                                                                       gTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATc     >  1:3820331/1‑140 (MQ=255)
                                                                                                                                       gTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGGTGGGGTAAATCAAGCAGATTTTGAGGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATc     >  1:7113462/1‑140 (MQ=255)
                                                                                                                                       gTTCAAATGCTTGCAAACGGAAAGACAAAAAAAGAGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATc     <  2:4403462/140‑1 (MQ=255)
                                                                                                                                       ctacAAATGCTCGCCAACGGAAAGACAAAAAATGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATc     <  1:4352159/137‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                            
TGTGGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAAC  >  NZ_CP034943/316124‑316401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: