Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 316,282 G→C 14.9% K34N (AAG→AAC opuAA → glycine/proline betaine ABC transporter ATP‑binding protein OpuAA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943316,2820GC14.9% 1710.3 / 68.2 702K34N (AAG→AACopuAAglycine/proline betaine ABC transporter ATP‑binding protein OpuAA
Reads supporting (aligned to +/- strand):  ref base G (402/166);  new base C (2/110);  total (407/295)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 706 total aligned reads displayed.

GTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAAC  >  NZ_CP034943/316143‑316420
                                                                                                                                           |                                                                                                                                          
 ttACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGTGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGa                                                                                                                                           >  1:979035/1‑140 (MQ=255)
 ttACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGa                                                                                                                                           >  2:1281525/1‑140 (MQ=255)
   aCGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGACAGCAGTTCAAATGCTTGCCAACGGAAAGACa                                                                                                                                         >  1:4502831/1‑140 (MQ=255)
   aCGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGa                                                                                                                                           >  2:6580974/1‑138 (MQ=255)
   aCGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACa                                                                                                                                         >  1:3259766/1‑140 (MQ=255)
   aCGGTTAGAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACACACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACa                                                                                                                                         >  1:1995331/1‑140 (MQ=255)
         ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGa                                                                                                                                           <  2:1777864/132‑1 (MQ=255)
         ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                                                   >  1:3344787/1‑140 (MQ=255)
         ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                                                   >  1:5353052/1‑140 (MQ=255)
           aaGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAACTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGATCAAATGCTTGCCAACGGACAGACAAAAAAaga                                                                                                                                 >  2:1178006/1‑140 (MQ=255)
                 tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTACGATAAAAGTCGAGTACGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGGCAACGGACCGACAAACTAAGAGATCCt                                                                                                                           >  2:4123592/1‑140 (MQ=255)
                 tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                                           >  1:4809980/1‑140 (MQ=255)
                 tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                                           >  2:2247022/1‑140 (MQ=255)
                 tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                                           >  2:2703627/1‑140 (MQ=255)
                 tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                                           >  1:6183430/1‑140 (MQ=255)
                 tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                                           >  1:678191/1‑140 (MQ=255)
                 tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGATTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                                           >  1:2239098/1‑140 (MQ=255)
                   tttAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAATAGATCCTGa                                                                                                                         >  2:2999342/1‑140 (MQ=255)
                   tttAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGa                                                                                                                         >  1:5492511/1‑140 (MQ=255)
                          tGGAGGCAAAATATGAGTGTAGATGATAAACAAATTAAGATAAAAGTCGAGAAATTATCTAAAATTTTTGGGAAACAAAAAAAGAAAGCAGTTCAAATGCTTGCCAACGCAACGACAAAAAAAGAGATCCTGAAAGCGAc                                                                                                                  <  2:7108572/140‑1 (MQ=255)
                           ggAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGAcc                                                                                                                 >  2:4214650/1‑140 (MQ=255)
                           ggAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGAcc                                                                                                                 >  2:723513/1‑140 (MQ=255)
                             atgtaaaaTATGAGTGTAGATGAGAAAAAAAAAAATATAAAAATCGAGAAAGAATATAAAATTTTTGTGAAACAAACAAATAAAGAATTTAAAATTCTTTCAAAATGAAAGACAAAAAAAGAGATACTGAAAGCGAACgg                                                                                                               >  2:3684115/5‑140 (MQ=255)
                             aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                               >  1:1005972/1‑140 (MQ=255)
                             aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                               >  1:3514273/1‑140 (MQ=255)
                             aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                               >  1:3101569/1‑140 (MQ=255)
                             aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                               >  1:2335539/1‑140 (MQ=255)
                             aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                               >  2:7300677/1‑140 (MQ=255)
                             aGGGAAAATATGAGTGTAGATGACAAACCAATAAAGATAAAAGTAGAGAAAGTATCTAACATTTTTGGGAAACAATCAAAGTACGCAGTTCCAATGCTTGCGCAGGGAAGGGCAAAATAAGGGGTCCTGCAAGCGAGCgg                                                                                                               >  2:4398642/1‑140 (MQ=255)
                                  aaaTATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACACACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCaa                                                                                                          >  1:504758/1‑140 (MQ=255)
                                     tatGAGTTTCGATGAGAAACAACTTAACAAAAACTACTACAATTTATCTAAATTTTTTGGCAAAAAAACAAAGAAACCTTTACAAATGCTGCCAAACCGAAACAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCg                                                                                                       <  1:7298949/140‑1 (MQ=255)
                                       tGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGtt                                                                                                     >  1:5742135/1‑140 (MQ=255)
                                         aGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgg                                                                                                   >  1:2848105/1‑140 (MQ=255)
                                           tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                                                 >  1:2538911/1‑140 (MQ=255)
                                           tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                                                 >  2:6096253/1‑140 (MQ=255)
                                           tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                                                 >  2:6429614/1‑140 (MQ=255)
                                           tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                                                 >  1:815535/1‑140 (MQ=255)
                                           tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                                                 >  1:644183/1‑140 (MQ=255)
                                           tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCATGAAAGCGACCGGATCAAACGTTgggg                                                                                                 >  1:7134522/1‑140 (MQ=255)
                                           tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGt                                                                                                >  1:1185859/1‑140 (MQ=255)
                                             tAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAATTAAGCAGTTCAAATGCTTGCCAACGGAACGACAAAAAAAGAGATCATGAAATCTACCGGATCAACCGTTGGGGTa                                                                                               >  2:5715104/1‑140 (MQ=255)
                                                aTGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAAt                                                                                            >  2:4497912/1‑140 (MQ=255)
                                                aTGAGAAACCAATTAAGATAAAAGACGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGACAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTaact                                                                                            >  2:5623517/1‑138 (MQ=255)
                                                     aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAATCAGTTAAAATGATTGCCAACGGAAAGACAAAAAAATAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                                       >  1:5183923/1‑140 (MQ=255)
                                                     aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCACCCGTTGGGGTAAATCAAGc                                                                                       >  1:2447869/1‑140 (MQ=255)
                                                     aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                                       >  1:3420853/1‑140 (MQ=255)
                                                     aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                                       >  1:6957279/1‑140 (MQ=255)
                                                     aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                                       >  1:1431284/1‑140 (MQ=255)
                                                     aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                                       >  1:4188934/1‑140 (MQ=255)
                                                     aaaCCAATTAAGATAAAAGTAGAGAAAGTATCTAAAATTTGTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTTCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTGGGGGTAAATAAAGc                                                                                       >  1:6621850/1‑140 (MQ=255)
                                                       aCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAg                                                                                     >  1:6344583/1‑140 (MQ=255)
                                                       aCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGGTGGGGTAAATCAAGCAg                                                                                     >  1:7024964/1‑140 (MQ=255)
                                                       aCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGGTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAg                                                                                     >  1:6110439/1‑140 (MQ=255)
                                                          aaTTAATATAAAAGTCGATAAATTATCTAAAATTTTTGGCAAACAAAAAAACAAAGCAGTTCAAAGGCTTGCCAACGGAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAtt                                                                                  <  2:2603778/140‑1 (MQ=255)
                                                           tttAAGATAACCGTAGAGACAGTATCTAAAATTTTTGGTCAAAAAACAAAGACATCAGTTCAAATGGTTGCCCCCGGAACGACAAAAAAAGAGATCGTTAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAttt                                                                                 <  2:4308326/139‑1 (MQ=255)
                                                             taacaaaaacGTCGAGAAAGTAACAAAAATTTTTGGCAAAAAAAAAAAGAAAGCAGTAAAAATGCTTGCCAACGCAAACACAAAAAAATAGAGCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                                               <  1:2388529/134‑1 (MQ=255)
                                                             tAATATAAAATTCTACAAAGTATCTAAAATTTTTGGCAAACAAACAAACAAAGAAGTAAAAATGCTGCCCACCGTAAAGACAAAAAAAGAGATCCTCAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                                               <  2:2531168/140‑1 (MQ=255)
                                                             tAATATAAAAGTCGATAAATTATCTAAAATTTTTGGCAAACAAACAAAGAAAGCAGTTCAAAGGCTTGCCACCGCAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                                               <  2:1431284/140‑1 (MQ=255)
                                                             tAATATAAAAGCCGAGAAATTATCAAAAATTTTTGGCAAACAAACAAACACAGCAGTTCAAAGGCTTGCCAACGCAAAGACAAAAAAACACATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                                               <  1:1619779/140‑1 (MQ=255)
                                                             tAAGATAAACGTCGACAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCTGTTATTCTGCTTGCCAAGGGAAAGAAAAAAAAATAGAGCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                                               <  1:7583019/140‑1 (MQ=255)
                                                             tAAGATAAAAGTCGATAACGTATATAAAATTTTTGGCAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAACAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                                               <  2:4648557/140‑1 (MQ=255)
                                                             tAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                                               <  2:3962074/140‑1 (MQ=255)
                                                             tAAGATAAAAGTCCACAAAGTATCTAAAATTTTTGGCAAACAAACAAAGAACGCCGTACAAATGCTTGCCAACGCAAACACAAAAAAAGACATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                                               <  2:3101569/140‑1 (MQ=255)
                                                             tAACATAAAAGTCGAGAAAGTATCTAAATTTTTTGGCAAACAAACAAAGAAAGCAGTTCAACTGCTGGCCACCGGAAAGACAAAAAAAGAGACCCTGCACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                                               <  2:3420853/140‑1 (MQ=255)
                                                             aaaGATAAAACTCCACAAATTATCTAAAATTTTTGGCAAACAAAAAAAGAATCCAGTAAAAATGCTTCCCACCGCAAATACAAAAAAAGAGAACCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                                               <  2:6981794/139‑1 (MQ=255)
                                                               acaTAAAAGTCGACAAATTATCTAAAATTTTGGGCAAACAAAAAAAGAACGCAGTTCAAATGCTTCCCAACGTAAACAAAAAAAAAGACATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                                             <  1:2703627/138‑1 (MQ=255)
                                                               aGATAAAAGTCTACACATTATCTAAATTTTTGGGCAAAAAAAAAAACACAGCAGTTCAAAGGCTTGCCACCGCAACCAAAAAAAAAGACATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                                             <  1:1861805/140‑1 (MQ=255)
                                                               aGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCGGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                                             <  2:235510/140‑1 (MQ=255)
                                                                   aaaaGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGtat                                                                         >  2:6820752/1‑140 (MQ=255)
                                                                    aaaGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAACCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGAGCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGtata                                                                        <  2:5124483/140‑1 (MQ=255)
                                                                          gataaaGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCATTTAAAATGCTTGCAAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATgg                                                                  <  2:4974534/137‑1 (MQ=255)
                                                                            taaaGTATCTAAAATTTTTGGGAAACAAAAAAAGAAAGCAGTTAAATTGCTTGCCAACGGAAAGAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTg                                                                <  2:6660217/139‑1 (MQ=255)
                                                                            gaAAGTATCTAAAATTTTTGGTAAACAAAAAAAGAAAGCAGTTAAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTg                                                                <  2:5502517/140‑1 (MQ=255)
                                                                            gaAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGCGATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTg                                                                <  2:6259553/140‑1 (MQ=255)
                                                                                  aTCTAAAATTTTTGGTAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGatat                                                          <  2:379444/140‑1 (MQ=255)
                                                                                    ctaatttttttGGCAAAAAACCAAACAACGCCGTTAAAATGCTGGCAACCGTAATAACAAAAAAAGTCCACCGGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATAttt                                                        <  1:3592771/134‑1 (MQ=255)
                                                                                     tAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTg                                                       <  2:3251992/140‑1 (MQ=255)
                                                                                           ttttGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                                             >  1:5603941/1‑112 (MQ=255)
                                                                                            tttGGGAACCAAACAAAGAAAGCTGTTCAATTGCTTGCCAACGGAAAGACAAAAAAAGTGAGCGGGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                                                <  2:1987721/140‑1 (MQ=255)
                                                                                            tttGGCAAAAAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGCAAACACAAAAAAAGACATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                                                <  2:589808/140‑1 (MQ=255)
                                                                                             ttGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATg                                               >  2:2811647/1‑140 (MQ=255)
                                                                                             ttGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATg                                               >  2:240631/1‑140 (MQ=255)
                                                                                               ggcaaaaaaacAAAGAAACCAGTTAAAATGGTTGCCAACGGAAACAAAAAAAAGACATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGt                                            <  1:582704/137‑1 (MQ=255)
                                                                                                ggAAACAAAAAAAGAAACCAGTACAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGt                                            <  2:3514273/140‑1 (MQ=255)
                                                                                                gcaaacaaaaAAAGAACGCAGTACAACTGCTGGCCAACGGAAACACAAAAAAAGAGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGt                                            <  2:4809980/138‑1 (MQ=255)
                                                                                                 taaacaaacAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTc                                           <  2:163833/139‑1 (MQ=255)
                                                                                                                agCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGAGGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTa                            >  1:4944558/1‑140 (MQ=255)
                                                                                                                agCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATGTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCATGGAGCGGTa                            >  1:4631569/1‑140 (MQ=255)
                                                                                                                    gTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGtcactc                        >  1:4002955/1‑135 (MQ=255)
                                                                                                                    gTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATc                        >  1:3820331/1‑140 (MQ=255)
                                                                                                                    gTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATc                        >  1:7232172/1‑140 (MQ=255)
                                                                                                                    ctacAAATGCTCGCCAACGGAAAGACAAAAAATGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATc                        <  1:4352159/137‑1 (MQ=255)
                                                                                                                               tGCCAACGGAACGACAAAACAAGAGATCCTGTACGCGACCGGATCAACCGTTGGGGTAACTCAAGCAGATATTCATGGAGATGAAGGTGAGATATTAGGCATCAGGGGAACAGAATGGAGAGGTAACACAACGTTAGttc             >  2:7728897/1‑138 (MQ=255)
                                                                                                                               tGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATTGGTCTATCAGGGAGCGGTAAATCAACTTTAGTAc             >  2:6345041/1‑140 (MQ=255)
                                                                                                                               tGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATTGGTCTATCAGGGAGCGGTAAATCAAATTTAGTAc             >  2:7602724/1‑140 (MQ=255)
                                                                                                                               tGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTAc             >  2:205143/1‑140 (MQ=255)
                                                                                                                               tGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTAc             >  2:525872/1‑140 (MQ=255)
                                                                                                                               tGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTAc             >  1:1743616/1‑140 (MQ=255)
                                                                                                                               tGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTAc             >  1:738599/1‑140 (MQ=255)
                                                                                                                               tGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAGTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGTGAGCGGTAAATCAACTTTAGTAc             >  1:130778/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
GTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAAC  >  NZ_CP034943/316143‑316420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: