Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 316,300 G→C 9.0% L40L (CTG→CTC opuAA → glycine/proline betaine ABC transporter ATP‑binding protein OpuAA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943316,3000GC 9.0% 1486.6 / 20.1 637L40L (CTG→CTCopuAAglycine/proline betaine ABC transporter ATP‑binding protein OpuAA
Reads supporting (aligned to +/- strand):  ref base G (331/207);  new base C (3/67);  total (335/302)
Fisher's exact test for biased strand distribution p-value = 5.50e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 637 total aligned reads displayed.

TTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCGGCTTATTGAACCGAC  >  NZ_CP034943/316162‑316437
                                                                                                                                           |                                                                                                                                         
tttAATATGGAGGGAAAATATTAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACACAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGa                                                                                                                                          >  1:4988556/1‑140 (MQ=255)
tttAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGa                                                                                                                                          >  1:5492511/1‑140 (MQ=255)
tttAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGa                                                                                                                                          >  2:7713037/1‑140 (MQ=255)
tttAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGa                                                                                                                                          >  1:7083986/1‑140 (MQ=255)
       tGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGAc                                                                                                                                   <  2:3730337/140‑1 (MQ=255)
          agtgAAAATATGAGTTTAGATGAGAAACCAATTAAGATACAAGTCGAGAAAGTATCTAAAATTTTTGCTAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACCGAAAGAAACAAAAAGAGATCCTGAAAGAGACCgc                                                                                                                                >  1:6401146/4‑139 (MQ=255)
          aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                >  1:3689953/1‑140 (MQ=255)
          aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                >  1:3101569/1‑140 (MQ=255)
          aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                >  1:2477572/1‑140 (MQ=255)
          aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                >  2:4010973/1‑140 (MQ=255)
          aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                >  1:234399/1‑140 (MQ=255)
          aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                >  1:2182561/1‑140 (MQ=255)
          aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                >  1:1005972/1‑140 (MQ=255)
          aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                >  1:5733847/1‑140 (MQ=255)
          aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                >  1:5663037/1‑140 (MQ=255)
          aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTAAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                >  1:6981794/1‑140 (MQ=255)
          aGGGAAAATATGAGTGTAGATGACAAACCAATAAAGATAAAAGTAGAGAAAGTATCTAACATTTTTGGGAAACAATCAAAGTACGCAGTTCCAATGCTTGCGCAGGGAAGGGCAAAATAAGGGGTCCTGCAAGCGAGCgg                                                                                                                                >  2:4398642/1‑140 (MQ=255)
          aGGGAAAATATGAGGGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                >  1:3274446/1‑140 (MQ=255)
                    tGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGtt                                                                                                                      >  2:567215/1‑140 (MQ=255)
                      aGTTTAGATCAGAAACCAATAAAGATAAAAGTATAGAAAGTATCTAAAATTGTTTGGAAACTAACAAAGAAAGCAGTTCAATTGCGAGGGAACGGAAAGAGAAAAAAGGAGTTCGTGAAATCGGCCCGATCTACCGTggg                                                                                                                    >  2:5422828/1‑140 (MQ=255)
                        tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                                                                  >  2:6455665/1‑140 (MQ=255)
                        tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                                                                  >  2:2380693/1‑140 (MQ=255)
                        tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGt                                                                                                                 >  1:1185859/1‑140 (MQ=255)
                         gtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGGTGGGGt                                                                                                                 >  2:4248628/1‑140 (MQ=255)
                          tAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTa                                                                                                                >  2:629509/1‑140 (MQ=255)
                             aTGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAAt                                                                                                             >  1:693555/1‑140 (MQ=255)
                             aTGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAAt                                                                                                             >  1:4911833/1‑140 (MQ=255)
                                agaAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGCTAAATCaa                                                                                                          >  2:205612/1‑140 (MQ=255)
                                  aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGAc                                                                                                                                   >  1:4399811/1‑113 (MQ=255)
                                  aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                                                        >  1:5344718/1‑140 (MQ=255)
                                  aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                                                        >  1:6086913/1‑140 (MQ=255)
                                  aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                                                        >  2:7258408/1‑140 (MQ=255)
                                  aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                                                        >  1:4040458/1‑140 (MQ=255)
                                  aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                                                        >  1:1675328/1‑140 (MQ=255)
                                  aaaCAAATTAATATAAAAGTCGATAAATTATCTAAAATTTTTGGGAAAAAAACAAATAAAGCATTTCAATGGCTTGCCAACGCAAACACAAAAAAAGACAGCGTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                                                        <  2:3071648/140‑1 (MQ=255)
                                    aCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAg                                                                                                      >  1:5174380/1‑140 (MQ=255)
                                    aCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAg                                                                                                      >  2:344568/1‑140 (MQ=255)
                                    aCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAg                                                                                                      >  2:6998710/1‑140 (MQ=255)
                                    aCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTGGGGGTAAATCAAGCAg                                                                                                      >  1:4544073/1‑140 (MQ=255)
                                       aaTTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGGTTGCCAACGGACAGACACAAAAAGAGTTCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAtt                                                                                                   >  1:2874583/1‑140 (MQ=255)
                                       aaTTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAtt                                                                                                   <  2:4490794/140‑1 (MQ=255)
                                       aaTTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAtt                                                                                                   >  2:4336565/1‑140 (MQ=255)
                                       aaTTAACATAAATGTCTAGAAATTATCTAAATTTTTTGGCAAACAAACAAAGAACGCATTTCAAAGCCTTGCCAACGGAAAGAAAAAAAAATAGACCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAtt                                                                                                   <  2:2924845/140‑1 (MQ=255)
                                          tAATATAAAAGTCGACAAAGTATATAAAATTTTTGGGAAAAAAACAAACAAACCAGTTCAAATGCTTCCCAACGCAAACAAAAAAAAAGAGAACCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                                                                <  1:7291928/140‑1 (MQ=255)
                                          tAATATAAAAGTCGACAAAGTAAATAAATTTTTTGGGAAACAAACAAAGAACGCAGTTCAAAGGCTTGCCACCGCAAACACAAAAAAACAGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                                                                <  1:2393154/140‑1 (MQ=255)
                                          tAAGATAAAAGTCGAGAAATTATCAAAAATTTTTGGCAAACAAACAAAGAACGCAGTTCAAATGCTTGCCAACGCAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                                                                <  1:7449708/140‑1 (MQ=255)
                                          tAAGATAAAAGACCAGACAGTATCAAAAATTTTGGGGAAACAAACAAAGAAAGCAGTTCAACTGCTTGCCACCGGAAAGACAAAAAAAGAGAACCGGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                                                                <  2:314900/140‑1 (MQ=255)
                                            ataTAAACGTCGACAAAGTATCTAAAATTTTTGCCAAAAAAACAAACAAACCCGTACAAATGCTTCCCAACGCAAACACAAAAAAAGAGAGCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                                                              <  1:2704305/138‑1 (MQ=255)
                                            acaTAAAAGTCTATAAAGTATCTAAATTTTTTGGGAAACAAACAAAGAAAGCAGTTAAAATGCTTGCCAACGCAAAGACAAAAAAAGAGATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                                                              <  2:3536103/138‑1 (MQ=255)
                                            aGATAAACTTCGAGAATCTATATAAAATTTTTGGGCAACAAACAAAGAAAGCCGTGCAACTGCTTGCCAACGGAAAGACAAAAAAAGACAGCCGGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                                                              <  2:1991247/140‑1 (MQ=255)
                                              aTAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGt                                                                                            >  1:2552355/1‑140 (MQ=255)
                                                aaaaGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGtat                                                                                          >  2:277369/1‑140 (MQ=255)
                                                 aaaGTCGAGAAAGTATCTAAAATTTTGGGCAAACAAACAAAGAAAGCATTTCAAATGCTTCCCAACGCAAAGAAAAAAAAAGAGAGCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGtata                                                                                         <  2:4630146/140‑1 (MQ=255)
                                                  aaGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTatat                                                                                        >  1:3424440/1‑140 (MQ=255)
                                                       tataacgTATATAAAATTTTTGGCAAACAAACAAAGAAAGAAGTTCAAATGCTTGCAAACGCAAAGAAAAAAAAAGACATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATgg                                                                                   <  1:6663044/134‑1 (MQ=255)
                                                       gataatttatataAATTTTTTGGGAAACAAACAAACAAAGCTGTTCAAATGCTTGCCAACGCAAAGAAAAAAAAAGAGATCCTCAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATgg                                                                                   <  1:1850195/133‑1 (MQ=255)
                                                       gataaaGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCATTTAAAATGCTTGCAAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATgg                                                                                   <  2:4974534/137‑1 (MQ=255)
                                                        tgaACGTATATAAATTTTTTGGTAAACAAAATAAGAAAGCAGTTCAAATGCTTGCCAACGGAACGACAAAAAAAGACATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGt                                                                                  <  2:1760434/139‑1 (MQ=255)
                                                        agaAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCTGTTCAAATGCTTGCCAACGCAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGt                                                                                  <  2:1204202/140‑1 (MQ=255)
                                                        agaAAGTATATAAAATTTTTGGCAAAAAAACAAAGAAAGCAGTTCAATTGCTTGCCAACGGAAACAAAAAAAAAGAGATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGt                                                                                  <  2:2639787/140‑1 (MQ=255)
                                                         caacttATCTAAATTTTTTGGCAAAAAAACAAAGAAACCATTTAAAATGGTTGCCACCGCAAAAAAAAAAAAAGAGAGCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTg                                                                                 <  1:875931/135‑1 (MQ=255)
                                                            ctaaTCTAAATTTTTTGGCAAACAAAAAAATTTAGAATTTCAAAGGATTGCCAACGTAAACAAAAAAAAAGAGTTCATGAAAGCGACCGGATCAACCTTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTgaga                                                                              <  2:2474700/137‑1 (MQ=255)
                                                               ctCTAAATTTTTTGGGAAACAAAAAACGAACGCAGTTCAAAGGGTTGCCACCGGATAAAAAAAAAAACACATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAAACAAGCAGATTTTGATGTATATGATGGTGAGatat                                                                           <  1:3531997/139‑1 (MQ=255)
                                                                 cTAAAATTTTTGGTAAAAAAACAAAGAAACCATTAAAAATCCTTGCCAACGCAAAGAAAAAAAAACAGATCCTGACAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATAttt                                                                         <  1:1490190/140‑1 (MQ=255)
                                                                   aaaaTTTTTGTTAAACAAACAAAGAAAGCAGTTAAAATGGTTGCCAACGGAAACACAAAAAAAGACATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGt                                                                       <  2:4811726/140‑1 (MQ=255)
                                                                         tttGGGAAAAAAAAAAAGAAAGCAGTTCAACTGCTTGCCACCGCAAAGAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                                                                 <  1:7624114/140‑1 (MQ=255)
                                                                         tttGGCAAACAAACAAACAAACCCGTTCAAAGGCTTCCCAACGCAAACACAAAAAAAGAGAGCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                                                                 <  1:6142198/140‑1 (MQ=255)
                                                                         tttGGCAAAAAAACAAAGAAAGCAGTTCAAATGCTTGCAAACGGAAACAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                                                                 <  2:3898611/140‑1 (MQ=255)
                                                                         ttgggcaaaaaaacAAATAACGCAGTCAAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCGGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                                                                 <  2:5110503/134‑1 (MQ=255)
                                                                         ttgggcaaaaaaacAAAGAAAGCAGTTAAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                                                                 <  2:1005972/134‑1 (MQ=255)
                                                                          ttGGGCAACCAAAAAACGAACGCAGTCCAAATGCTGGCCAACGGAAAGAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                                                                 <  2:5240943/140‑1 (MQ=255)
                                                                           gggtaaaaaaacAAAGAAAGCATTTAAAATGCTTCCCAACGCAAACACAAAAAAACACATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATgg                                                               <  1:3844303/136‑1 (MQ=255)
                                                                             ggAAACAAACAAAGAAAGCAGTTCAACTGCTTGCCAACGGAAACACAAAAAACGAGAGCCTGAAAGCGACCGGATCAACCGTTGGGGTAACTCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGt                                                             <  2:2555625/140‑1 (MQ=255)
                                                                             gcaaactaacAATGAAAGCACTCCAAAGGCTTGCCAACTGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCATGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGt                                                             <  1:7367217/138‑1 (MQ=255)
                                                                              gcacaaaaacacaCACAGCAGTGCAAATCCTTGCCAACGCAAAGACAAAAAAAGAGCGCCGGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGt                                                             <  2:137142/134‑1 (MQ=255)
                                                                                       caaaGAACGCCGTACAAATGGTTCCCACGGTAAAGAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAggg                                                    <  1:421683/140‑1 (MQ=255)
                                                                                          agaaagCATTTCAAATGCTTGCCAACGCAAATAAAAAAAAACAGAGCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGc                                                 <  1:5826516/140‑1 (MQ=255)
                                                                                           caatgatgTTCAAATGCTTGCAAACGTAAACAAAAAAAAAGAGATCATTAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCg                                                <  1:3336302/133‑1 (MQ=255)
                                                                                              agCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTGGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTa                                             >  1:7215691/1‑140 (MQ=255)
                                                                                               gCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTaa                                            <  2:3330801/140‑1 (MQ=255)
                                                                                                 actgcAAATGCTTGCCACCGGAAAGACAACAAAAGAGATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAAt                                          <  2:6934002/136‑1 (MQ=255)
                                                                                                   ttCAAATGCTTGCCAACGGACAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTGGGGGTAAATCAAGCAGTTTTTGATGTCTATGATGGTGAGATATTTGTCAGCATGGGTCTATCAGGGAGCGGTAAATCa                                        >  2:4132150/1‑140 (MQ=255)
                                                                                                    tCAAATGCTTGCCAACGAAAAGAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCaa                                       <  2:575593/140‑1 (MQ=255)
                                                                                                     cAATTGCTTCCAAACGTAATGAAAAAAAAAGTGTTCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAAc                                      <  2:613370/140‑1 (MQ=255)
                                                                                                     cAAATGCTTGCCAACGCAAAGACAAAAAAAGAGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAAc                                      <  1:1981534/140‑1 (MQ=255)
                                                                                                     cAAATGATTCCCAACGTAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAAc                                      <  2:6620888/140‑1 (MQ=255)
                                                                                                            ttGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                                                              <  2:1458393/77‑1 (MQ=255)
                                                                                                             tGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCTGGGGTGTATAAGGGAGCGGTAAATCAACTTTAGTAc                              >  2:7755768/1‑140 (MQ=255)
                                                                                                             tGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATTGGTCTATCAGGGAGCGGTAAATCAACTTTAGTAc                              >  2:6345041/1‑140 (MQ=255)
                                                                                                             tGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTAc                              >  1:4684530/1‑140 (MQ=255)
                                                                                                             tGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAGTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGTGAGCGGTAAATCAACTTTAGTAc                              >  1:130778/1‑140 (MQ=255)
                                                                                                                       aaGAAAAAAAACGAGATCCGCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTaaa                    <  2:6760179/140‑1 (MQ=255)
                                                                                                                        aGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATTATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAAc                   <  2:7144798/140‑1 (MQ=255)
                                                                                                                        aGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAAc                   >  2:554918/1‑140 (MQ=255)
                                                                                                                          aCAAAAAAAGAGATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCg                 <  1:6338697/140‑1 (MQ=255)
                                                                                                                              aaaaaGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCGGCtt             >  2:5927974/1‑140 (MQ=255)
                                                                                                                              aaaaaGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCGGCtt             >  2:7501154/1‑140 (MQ=255)
                                                                                                                                      aTCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCGGCTTATTGAAcc     >  2:4684251/1‑140 (MQ=255)
                                                                                                                                      aTCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCGGCTTATTGAAcc     <  2:2254466/140‑1 (MQ=255)
                                                                                                                                         cTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCGGCTTATTGAACCGAc  <  2:1150657/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
TTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCGGCTTATTGAACCGAC  >  NZ_CP034943/316162‑316437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: