Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 438,766 G→T 5.1% intergenic (‑32/‑74) gabR ← / → gabT transcriptional regulator GabR/4‑aminobutyrate‑‑2‑oxoglutarate transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943438,7660GT 5.1% 1042.2 / 8.8 370intergenic (‑32/‑74)gabR/gabTtranscriptional regulator GabR/4‑aminobutyrate‑‑2‑oxoglutarate transaminase
Reads supporting (aligned to +/- strand):  ref base G (219/118);  new base T (2/31);  total (221/149)
Fisher's exact test for biased strand distribution p-value = 1.67e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 370 total aligned reads displayed.

TGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTC  >  NZ_CP034943/438628‑438902
                                                                                                                                          |                                                                                                                                        
 gtgtCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGtt                                                                                                                                        <  2:4634485/140‑1 (MQ=255)
 gtgGCAGCAGATTACGGCTGAGAATTTCTTTTTTTCGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGtt                                                                                                                                        <  2:4887721/140‑1 (MQ=255)
 gtgGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGtt                                                                                                                                        <  1:3930184/140‑1 (MQ=255)
 gtgGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGtt                                                                                                                                        <  2:2736452/140‑1 (MQ=255)
  agcaagaagATAACGGCTGAGAATTTCTTTTTTAAGTAAATGATAAATTTGATGATATAAATAATCGGCTTCTTCTTAGAGGTCTAGATTCAATGTGATGACAATTTGAATATTCTCCTTCTGGTACAATCAAAAAGTta                                                                                                                                       >  2:5238023/8‑140 (MQ=255)
   ggCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGTCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTAAAATCAAAAAGTtat                                                                                                                                      >  1:6362197/1‑140 (MQ=255)
   ggCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTtat                                                                                                                                      >  2:3880290/1‑140 (MQ=255)
   ggCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTtat                                                                                                                                      >  1:3543223/1‑140 (MQ=255)
   ggCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTtat                                                                                                                                      >  1:240023/1‑140 (MQ=255)
       gcagATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAAtt                                                                                                                                  <  1:1784072/140‑1 (MQ=255)
        cagATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTg                                                                                                                                 >  2:4519844/1‑140 (MQ=255)
         agATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTgg                                                                                                                                <  2:3684075/140‑1 (MQ=255)
             tACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACt                                                                                                                            >  2:328079/1‑140 (MQ=255)
             tACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACt                                                                                                                            >  2:4309975/1‑140 (MQ=255)
               cGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACttt                                                                                                                          >  2:6421706/1‑140 (MQ=255)
                  cTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTtca                                                                                                                       >  1:4612301/1‑140 (MQ=255)
                    gagaATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTAGCATCAAAAAGTTATAATTGGTACTTTtcatc                                                                                                                     >  1:3083199/1‑140 (MQ=255)
                    gagaATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTtcatc                                                                                                                     <  1:7383051/140‑1 (MQ=255)
                    gagaATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTtcatc                                                                                                                     <  1:615692/140‑1 (MQ=255)
                     agaATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATCATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAACAGTTATAATTGGTACTTTtcatca                                                                                                                    >  1:2598284/1‑140 (MQ=255)
                     agaATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAACAAGTTATAATTGGTACTTTtcatca                                                                                                                    >  2:4790941/1‑140 (MQ=255)
                     agaATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGtt                                                                                                                                        <  1:5025963/120‑1 (MQ=255)
                     agaATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTtcatca                                                                                                                    >  1:7334247/1‑140 (MQ=255)
                               tttttAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAaag                                                                                                          >  1:2252298/1‑140 (MQ=255)
                                ttttAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAaaga                                                                                                         <  2:5011686/140‑1 (MQ=255)
                                      ttttcGCTAAATTTGCTGATAGATAGAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAACAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAg                                                                                                   <  1:7762234/140‑1 (MQ=255)
                                       ttttGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGa                                                                                                  <  2:7334247/140‑1 (MQ=255)
                                        tttGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGaa                                                                                                 <  2:6995828/140‑1 (MQ=255)
                                             tAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTa                                                                                            <  2:6355147/140‑1 (MQ=255)
                                                       tatagataTAATCGGTTTTTTTTTAGGGGTCGAGTTTTAATTTGTTGTCAATTTGAATATTCTCCTTCTGGTACAATAAAAAATTTATAATTGGTACTTTTCATAATACCAAAAATAATTCACATTGGTAAGAAAATAGc                                                                                  <  2:2840530/139‑1 (MQ=255)
                                                       gttagctaTATTCGGAGTGTTCTGTGCGGTCGAGTGTGTTGTTGTTGTCCATTTGAAGTTTCTCGTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATCCCAAAAATATGTCAGAATGGTAAGAAAATCGc                                                                                  <  2:1907842/138‑1 (MQ=255)
                                                         tatatatAATCGGCTTTTTCTGTGCGGTGGAGTGTGAATGTTTTGTCCTTTTGCTTATTGTCATTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAACAAGTAAGATTGGTAAGAAAATAGCGa                                                                                <  2:4908770/137‑1 (MQ=255)
                                                         tatatatAAGGGGTTTGGTCTGAGGGGGCGAGTGTGAATTTTATGGCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAATAAGTCAGAATGGTAAGAAAATCGCGa                                                                                <  2:4067952/137‑1 (MQ=255)
                                                          agaaaTAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTAAATAATACAAAAAAGAAGTCAGAATGGTAAGAAAATAGCGAc                                                                               <  2:7053918/140‑1 (MQ=255)
                                                           tatatAATCGGGTTTTTCTGAGGGGTCTTGTGTGAATGTTATTTCCATTTGCAGTTTCTCCTTCTGGTACCATAAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACa                                                                              <  2:7183348/139‑1 (MQ=255)
                                                           gataTAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACa                                                                              >  2:5904366/1‑140 (MQ=255)
                                                            atttaATCGGCTTTTTCGGAGCGTTCGAGTGTGAATGTGATGTCCTTTTGCCTATTCTCCTTCTGTTACCATCAAAAATTTATAATTGGTACTTTTCATAATACCAAAAATAAGTCATATTGGTAAGAAAATAGCGACAg                                                                             <  2:605265/137‑1 (MQ=255)
                                                            atatAATGGGGTTGTTGTGCGCGGTCGCGTGTGATTTTGATGTCCATTTGCATATTCTCCTTCTGGTACCATAAAAAAGTTATAATTGGTACTTTTCATCATACAAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAg                                                                             <  2:6723620/140‑1 (MQ=255)
                                                            atatAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAg                                                                             >  2:3764366/1‑140 (MQ=255)
                                                              ataATCGGCTTGTTCTGAGCGGTCGAGTGTGAATTTGATGTCCATTTGAAGATTCTCCTTCTGGTACAATAAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAggg                                                                           <  2:2026865/140‑1 (MQ=255)
                                                                  tCGGCTTGTTCTGTGCGGTCGAGTGTGAATGTGATGTCATTTTGCAGTTTCTCTTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAATTCAGAATGGTAAGAAAATAGCGACAGGGGGAc                                                                       <  2:1508932/140‑1 (MQ=255)
                                                                  tCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAATTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGAc                                                                       >  2:1816158/1‑140 (MQ=255)
                                                                    ggCTTGTTCTGAGCGGTCGAGTTTGAATGTTATGTCAATTTGCAGATTCTCCTTCTTGTAACATAAAAAATTTTTAATGGGTACTTTTCATCATACAAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACAt                                                                     <  2:7545973/140‑1 (MQ=255)
                                                                     gCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAAAAGCGACAGGGGGACATa                                                                    >  1:5665938/1‑140 (MQ=255)
                                                                      cTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATAc                                                                   >  1:7634935/1‑140 (MQ=255)
                                                                       ttGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACg                                                                  >  2:2519466/1‑140 (MQ=255)
                                                                         ttcgtGAGCGGTCTTGTTTCATTGTGATGCCATTTTGCAGATTCCCCTTCGGGACCCAAAAAAATTTTATATTTGGAACTTTAAAACACCCAAAAAAGAAGTCAGATTGGTAACAAAATAGCGACAGGGGGACATACGCa                                                                <  1:3261235/136‑1 (MQ=255)
                                                                          ttCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGCCATACGCAt                                                               >  2:789775/1‑140 (MQ=255)
                                                                             tGAGGGGTCGAGTGTGATTGTGATGTCCTTTTGCAGATTCTCCTTCTGGTACAATAAAAAATTTATATTTGGTACTTTTCATCATACCAAAAAGAAGTCATAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAg                                                            <  2:1233180/140‑1 (MQ=255)
                                                                             tGAGCGGTCGTGTGTGAATGTGCTGTCAATTTGCATTTTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAg                                                            <  2:2412664/140‑1 (MQ=255)
                                                                               aGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTc                                                          >  2:5550589/1‑140 (MQ=255)
                                                                               aGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTc                                                          >  1:661609/1‑140 (MQ=255)
                                                                               aGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTc                                                          >  1:361424/1‑140 (MQ=255)
                                                                               aGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTc                                                          >  2:4828506/1‑140 (MQ=255)
                                                                               aGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATCAGTc                                                          >  2:4431539/1‑140 (MQ=255)
                                                                                gCGGTCGTGTGTGATTTTTATGTCCTTTTCCATATTATCCTTCTGGTACAAAAAAAATTTTATATTTGTTAGTTTTAAACAAACAAAAAATAATTCACATTGTTAACAAAAAAGCGACAGGGGGACATACGCATGAGTCa                                                         <  1:678870/140‑1 (MQ=255)
                                                                                   gTCGAGTGTTAATGTGTTGTCATTTTGCATATTCTCCTTCTGTTACCATAAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaac                                                      <  2:1140584/140‑1 (MQ=255)
                                                                                    tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                     >  1:1006611/1‑140 (MQ=255)
                                                                                    tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                     >  1:4723703/1‑140 (MQ=255)
                                                                                    tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                     >  1:1382961/1‑140 (MQ=255)
                                                                                    tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                     >  1:4425711/1‑140 (MQ=255)
                                                                                    tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                     >  1:2096911/1‑140 (MQ=255)
                                                                                    tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                     >  1:2691063/1‑140 (MQ=255)
                                                                                    tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTAATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                     >  2:3935905/1‑140 (MQ=255)
                                                                                    tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCGGGTACCATCAAAAAGTTCTAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAAGGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAatca                                                     >  1:825095/1‑140 (MQ=255)
                                                                                       attgtgAATGTGATGTCCTTTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGACTGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAacaaca                                                  <  2:7687942/138‑1 (MQ=255)
                                                                                          tttattgtgATGTCCTTTTGCATTTTCTCCTTCTGGTACAATCAAAATTTTATATTTGGTATTTTTCATCATACCAAAAAGAAGTAATATTGTTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACagca                                               <  1:5998099/135‑1 (MQ=255)
                                                                                          gttattttgATGTCCATTTGCAGATGCTCCTTCTGTTACAATAAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAACAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACagca                                               <  2:3543220/133‑1 (MQ=255)
                                                                                          gttattgtgATGTCCATTTGCATTTTCTCCTTCTGGTACCATAAAAAATTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACagca                                               <  2:661609/135‑1 (MQ=255)
                                                                                          gttattgtgATGTCATTTTGCTTATTCTCCTTCTGTTAACATAAAAATTTTATATTTGGTATTTTTAAAAATACAAAAAATAAGTCAGAATGGTAACAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACagca                                               <  1:88771/135‑1 (MQ=255)
                                                                                              aTGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACagcaagca                                           >  2:4037328/1‑140 (MQ=255)
                                                                                                gtgATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATc                                         >  2:915978/1‑140 (MQ=255)
                                                                                                 ttaTGTCATTTTGAAGATTCTCCTTCTGTTACCAAAAAAAAGTTATAATTGGTACTTTTCATCATACAAAAAAGAAGTCAGAATGGTAACAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCa                                        <  1:4309975/138‑1 (MQ=255)
                                                                                                   aTGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAgggg                                                                          >  1:4853521/1‑104 (MQ=255)
                                                                                                   aTGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACg                                      >  2:3166114/1‑140 (MQ=255)
                                                                                                   aTGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACg                                      >  1:5231815/1‑140 (MQ=255)
                                                                                                     gTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGAc                                    >  1:1237851/1‑140 (MQ=255)
                                                                                                     gTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGAc                                    >  1:3394179/1‑140 (MQ=255)
                                                                                                        cgtttGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGc                                 >  1:5505031/3‑140 (MQ=255)
                                                                                                        cATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTCAGAAAATAGCGACACGGGGACATACGCATGAGTCAAACAACACCAAGCATCACGACttc                                 >  2:6180431/1‑138 (MQ=255)
                                                                                                        cATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACACCAGCAAGCATCACGACTGc                                 >  1:6947918/1‑140 (MQ=255)
                                                                                                        cATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGAGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGc                                 >  2:4228587/1‑140 (MQ=255)
                                                                                                        cATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTATTCATCATACCAAAAAGAAGTAAGAATGGTAAGAAAATAGCGACAGGGGTACATACTCATGAGTCAAACAACAGCAAGCATCACGACTGc                                 >  2:5091015/1‑140 (MQ=255)
                                                                                                           ttGCATTTTCTCCTTCTGGTACCATAAAAAAGTTATAATTGGTACTTTTCATCATCCAAAAAAGAAGTCAGAATGGAACGAAACTAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCa                              <  2:3017405/140‑1 (MQ=255)
                                                                                                                   tctcCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAATTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAAAAGAAAGCATCACGACTGCCCAGTGgcaga                      >  2:1274172/1‑139 (MQ=255)
                                                                                                                     tcCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCaa                    >  2:1708340/1‑140 (MQ=255)
                                                                                                                          cTGGTACCAACAAAAAGTTATATTTGGTACTTTTCATCAGACAAAAAACACGTCAGACTGGAAAGAAAATCGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACg               <  1:2856856/140‑1 (MQ=255)
                                                                                                                           tGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCCTGAGTCCAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACgg              >  1:1530229/1‑140 (MQ=255)
                                                                                                                             gTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGa            >  2:2637361/1‑140 (MQ=255)
                                                                                                                             gTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGa            >  2:6810229/1‑140 (MQ=255)
                                                                                                                               aCCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGc          >  2:1037741/1‑140 (MQ=255)
                                                                                                                                 caaaaaaaaTTTATAATTGGTACTTTCCATCAACCCAAAAATAACTCAGACGGGAACGAAAAGAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCaa        <  1:2978525/136‑1 (MQ=255)
                                                                                                                                 cATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAGAGAAGTCAGAATGATAAGAAAATACCGACAGGGGGATATTCACATGAGTCAAACAACAGCAAGCATCACAACTGCACAATGGCAGCAAAAACGGGATCaa        >  2:5365195/1‑140 (MQ=255)
                                                                                                                                   tCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAAtt      >  2:2918230/1‑140 (MQ=255)
                                                                                                                                   tCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATCCGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAACCGGGAGCCAtt      >  2:6303766/1‑140 (MQ=255)
                                                                                                                                    cAAAAATTTATAATTGTTACTTTTCAAAATACCAAAAAGAAGTAAGATTGGTAACAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTc     <  1:3734283/140‑1 (MQ=255)
                                                                                                                                    aaaaaaGTTATATTGGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTc     <  2:2208940/139‑1 (MQ=255)
                                                                                                                                    aaaaaaGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCATAATGGTAATAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTc     <  2:578632/139‑1 (MQ=255)
                                                                                                                                    aaaaaaGTTATAATTGGTACTTTACATCATACCAAAAATAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTc     <  1:1496605/139‑1 (MQ=255)
                                                                                                                                       aaaGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGtc  >  1:6915287/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                        
TGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTC  >  NZ_CP034943/438628‑438902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: