Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 549,119 T→G 9.4% L44R (CTG→CGG)  EO946_RS02865 → PLP‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943549,1190TG 9.4% 1099.0 / 17.5 463L44R (CTG→CGG) EO946_RS02865PLP‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base T (241/152);  new base G (2/66);  total (244/219)
Fisher's exact test for biased strand distribution p-value = 6.62e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 463 total aligned reads displayed.

CAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAAT  >  NZ_CP034943/548981‑549257
                                                                                                                                          |                                                                                                                                          
caacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCACAAGACTCCTg                                                                                                                                           >  2:863515/1‑140 (MQ=255)
caacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTg                                                                                                                                           >  1:7761645/1‑140 (MQ=255)
 aacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGTAGCTCCCTTACAAAAGACACCTgg                                                                                                                                          >  2:7683560/1‑140 (MQ=255)
 aacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTgg                                                                                                                                          >  1:4970132/1‑140 (MQ=255)
   cccAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCg                                                                                                                                        >  2:3217048/1‑140 (MQ=255)
        aTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGACGCTCCCTTCCAAAAGACTCCTGGCGAGTCa                                                                                                                                   >  1:6866812/1‑140 (MQ=255)
           gATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCTGCt                                                                                                                                >  1:6394765/1‑140 (MQ=255)
           gATATCACGCCTTTTTTGAATAGAAATCGGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCt                                                                                                                                >  1:5343885/1‑140 (MQ=255)
             tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                                                              >  1:1277692/1‑140 (MQ=255)
             tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                                                              >  1:1716148/1‑140 (MQ=255)
             tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                                                              >  2:5296540/1‑140 (MQ=255)
             tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                                                              >  2:2079479/1‑140 (MQ=255)
             tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                                                              >  2:669905/1‑140 (MQ=255)
              atCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTc                                                                                                                             >  1:3969787/1‑140 (MQ=255)
              atCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTc                                                                                                                             >  2:1936555/1‑140 (MQ=255)
              atCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTc                                                                                                                             >  2:5111846/1‑140 (MQ=255)
              atCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTc                                                                                                                             >  2:6492555/1‑140 (MQ=255)
                  cGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATc                                                                                                                         <  2:2365663/140‑1 (MQ=255)
                  cGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATc                                                                                                                         >  1:952917/1‑140 (MQ=255)
                     cTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGt                                                                                                                      >  1:27187/1‑140 (MQ=255)
                         ttttAATATAAATCTGTATTTTCCGATTTATCAGCAGATTTATCGATATTTCAAAGAAAACATGAATCCGGGGCGTTTTCAAAAAGGATTGAAGCTCCCTTCCAAAATACTCCTGGGGATTCTGCTTTCCATCAGTCaaa                                                                                                                  <  2:3749997/140‑1 (MQ=255)
                         ttttAATATAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAACCATGCATCTGGGGCTTTTTCAAAAAGCATTGAAGCTCCCTTCCAAACCACCCCTGGCGAGTCAGCTTTCCATCAGTCaca                                                                                                                  <  2:3432539/140‑3 (MQ=255)
                         tttGAATAGAAATCTGTATATTCCGCTTTATCCGCAGCTTTATCGATTTTTCAAAGAAAACATCCATCAGGGGCGTATTCAAAAAGGAATGACGCTCCCTTCCAAACCACGCCTGGCGAGTCAGCTTTCCATCCGTCaca                                                                                                                  <  2:5858182/140‑3 (MQ=255)
                          tttaaTATAAATTTTGATTTTCGGATTTATCAGCTGCTTTATCGATATTTAAAATAAAACTTGATTCAGGGGCGTTTTAAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAAc                                                                                                                 <  2:1594575/137‑1 (MQ=255)
                          tttaaTATAAATGTGTATATTCCGCTTTATCAGCAGCTTTATCGATATTTAAAATAAAACATGCATCAGGGGCGTATTAAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAAc                                                                                                                 <  2:7109639/137‑1 (MQ=255)
                          tttaaTAGAAATGTGTATATTCCGCTTTAGCAGCAGCTTTATCGCTATTTCAAAGAAAAAATGCATCAGGGGCTTATACAAAAAGGAATGACGCTCCCTTCAAAAACACTCCGGGCGAGTCAGCTTTCCAACAGacacac                                                                                                                 <  2:1425855/137‑1 (MQ=255)
                          ttGAATAGAAATCTGGATATTCCGCTTTATAAGCAGCTTTATCGATATTTAAAAGAAAACATGCATCAGGGGCGTATTAAAAAAGGAATGAAGCGCCCTTCCAAAACACCCCGGGCGAGTCAGCTTTCCACCAGTCAAAc                                                                                                                 <  2:4603890/140‑1 (MQ=255)
                               tAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTg                                                                                                            >  2:2191263/1‑140 (MQ=255)
                               tAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTACATCAGTCAAACGACTg                                                                                                            >  2:5510213/1‑140 (MQ=255)
                               tAGAAAACTCCAGATTCCGCTTTATCAGCATCTTTATAGCTAATACAAAGAAAACATGCATCAGCGGCCAATTCAAAAACGAATGAAGCTCCCTTCCAAAAGACTCCTGGAGAGTCCCCTTTCCAGCAGTCCAACGagtc                                                                                                            >  2:6175668/1‑137 (MQ=255)
                                  aaaTCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAg                                                                                                         >  2:17840/1‑140 (MQ=255)
                                      cttgATATTCCGTTTTATCAGCAGATTTATCGCTATTAAAAATAAAACATGAATCCGGGGTTTATTAAAAAAGTAATTAAGCTCCCTTCCAAACGACTCCGGGCGAGTCAGGTTTCCATCAGTCAAACGACTGTAGAACg                                                                                                     <  2:6511216/137‑1 (MQ=255)
                                      cggtatatTCCGCTTTATCCGCAGCTTTATCGCTATTGCAAACAAAACATGCAGCAGGGGGGTATTCAAAAAGCAATGAAGCGCCCTTCCAAACGACGCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACg                                                                                                     <  1:4148305/136‑1 (MQ=255)
                                      cTGTATTTTCCGCTTTATCTGCTGCTTTATCGATATTTCAAATAAAACATGCATCAGGGGCTTATTAAAAAAGGAATGACGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGACAAACGACTGTAGAACg                                                                                                     <  2:7310076/140‑1 (MQ=255)
                                      cTGTATATTCCGCTTTATCCGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCCGGGGCTTTTACAAAAAGGAAGGACGCGCCCTTCCAAAAGACGCCGGGGGCGTCCGCTTTCCATCAGACACACGACTGTAGAACg                                                                                                     <  1:1579847/140‑1 (MQ=255)
                                      cTGTATATTCCGCTTTATCAGCAGCTTTATCGATATTACAAACAAAACATGCATCCGGGGCGTATTCAAAAAGGAATTAAGCTCCCTTCAAAAACACGCCTGGCGAGTCAGGTTTCCACCAGAAACACGACTGTAGAACg                                                                                                     <  2:430187/140‑1 (MQ=255)
                                      cTGTATATTCCGCTTTATCACCAGATTTATCGTTATTTCAAATAAAACATGAATCCGGGGCGTATTCAAAATGTAATGAAGCTCCCTTCCAAAACACTCCTGGCGAGTCAGCTTTCCATCACAAAAACGACTGTAGAACg                                                                                                     <  2:3872595/140‑1 (MQ=255)
                                      cTGGATATTCCGATTTACCCGCAGGTTTATCGATATTACAAAGAAAACATGCACCCGGGGGGTTTTCAAAAAGGAATGAAGCTCCCTCCAAAAAGACGCGGGGCGAGTCAGGTTGCCATCCGTCAAACGACTGTAGAACg                                                                                                     <  2:6840459/140‑1 (MQ=255)
                                       tGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAAAGt                                                                                                    >  2:3572078/1‑140 (MQ=255)
                                         gATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGc                                                                                                  >  1:4800892/1‑140 (MQ=255)
                                         gATATACCCATTTAACACCAGCTTTAGCGATATTACAAACAAAACAGGCAACCGGGGCGTATTCAAAACGGAATGAAGCTCCCTTCCAACCCACGCCTGGGTAGTCCGCTTTCCACCAGTCAACCGACTGTAGAACGTGc                                                                                                  <  1:5629413/140‑1 (MQ=255)
                                              tCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTCTTCAAAAAGGAATGAAGCTCCCTTCCAAAAGAGTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATg                                                                                             >  1:361414/1‑140 (MQ=255)
                                                cgatttATAAGCATGTTTTTCGCTATTTAAAAAAAAACATGCATCAGGGGCGTATTAAAAAAGTAATTAAGCTCCCTTCAAAACGCGTCCGGGGGTGTCAGCTTTCCTTCAGTAACACGACTGTAGAACGTGCCTATGaa                                                                                           <  1:305523/137‑1 (MQ=255)
                                                cGCTTTATCAGCAGCTTTATCGATATTACAAACAAAACATCCATCCGGGGCTTATTAAAAAAGGAATCAAGCTCCCTTCCAAAACCCGCCTGGCGAGTCAGCTTACCATCAGTAAAACGACTGTAGAACGTGCCTATGaa                                                                                           <  1:7541565/140‑1 (MQ=255)
                                                 gCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGaaa                                                                                          >  2:3990954/1‑139 (MQ=255)
                                                    ttATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGc                                                                                       >  1:2092658/1‑140 (MQ=255)
                                                     tATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCt                                                                                      >  1:2532043/1‑140 (MQ=255)
                                                       tCAGCAGCTTTCTCGCTATTACAAATAAAACATGCATCCGGGGCTTATTCAAAAAGGAATGACGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCACCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTtg                                                                                    <  2:3617643/140‑1 (MQ=255)
                                                            cgcTTTATCGATATTACAACCAAAACATCAATCCGGGGCGTTTCCAAAACGGAATGAAGCGCCCTACCAAAACACACCGGGCGAGTCAGCTTCCCAACCGACACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                               <  1:3122565/139‑1 (MQ=255)
                                                            agcTTTATCTAAATTTCAAACAAAACCTCCACCCGGGCCGTATTCAAAAAGCATTTAAGCCCCCTTCCAAAACACACCTGGCGAGTCAGCTTTACACCAGACACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                               <  2:2964891/140‑1 (MQ=255)
                                                            agcTTTATCGCTATTTCAAACAAAACATGCATCCGGGGCGTATTAAAAAAGGAATGACGCTCCCTTCCAAAACACGCGGGGCGAGTCAGCTTGCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                               <  2:28545/140‑1 (MQ=255)
                                                            agcTTTATCGCTATTTCAAACAAAACATGCACCAGGGGCGTATAAAAACCGGAATCAAGCTCCCTCCCAAAACCCTCCGGGCTAGTCAGCTTGCCATCACACACACGACTGTAGAACGTGCCTATGACCAGCTTgctgct                                                                               <  1:1357057/140‑1 (MQ=255)
                                                            agcTTTATCGCTATTTCAAACAAAAAATGCATCCGGGGCGTATTAAAAAAGGAATGACGCTCCCTTCCAAACCACGCCTGGCGAGTCAGCTGTCCACCAGACACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                               <  2:2532043/140‑1 (MQ=255)
                                                            agcTTTATCGATTTTTCAAACAAAAAATCCATCCGGGGCGTATTAAAAACGGAATGAAGCTCCCTTCCAAACGACTCCGGGCGAGTCAGCTTTCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                               <  1:5448953/140‑1 (MQ=255)
                                                            agcTTTATCGATATTTCAAAGAAAACATGCACCCGGGGCTTATTCAAAAAGGAATTATGCGCCCTTCCAAAACACTCCTGCCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                               <  2:6604830/140‑1 (MQ=255)
                                                            accTTTATCGCTATTTCAAACAAAACATGCATCCGGGGCTTATTAAAAAAGTAATGAAGCTCCCTTCCAAAACACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                               <  2:4205228/138‑1 (MQ=255)
                                                              cTTTATCGCTATTACAAACAAAACATCCATCCGGGGCTTATAAAAAAAGCACTGAAGCTCCCTACCAACACACCCCGGGCGAGTCAGCTTTCCAACAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                             <  1:3375259/140‑1 (MQ=255)
                                                              cTTTACCCCTATTACAAACAAAACAGCCATCCGGGGCGTATACAAAAAGGAATCAAGCTCCCTTCCAAACCACCCCTGGCGAGTCAGCTTCCCAACAGACACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                             <  1:6962747/140‑1 (MQ=255)
                                                                 tATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAggg                                                                          >  2:6375350/1‑140 (MQ=255)
                                                                   tctcTATTTAAAAGAAAACATCCATCAGGGGCTTATAAAAAAAGGATTGAAGCTCCCTTCCAAAAGACTCCGGGCGTGTCTGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCt                                                                        <  2:1240980/137‑1 (MQ=255)
                                                                   tcccTATTACAAACAAAACATGCATCCGGGGCTTATTCAAAAAGGAAGGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCt                                                                        <  1:7676530/137‑1 (MQ=255)
                                                                   tCGCTATTACAAACAAAAATTGCATCAGGGGCGTATACAAAAAGGATTGAAGCTCCCTTCCAAAACACTCCTGCCGAGTCAGCTTTCCATCACTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCt                                                                        <  1:6206557/140‑1 (MQ=255)
                                                                        atatCAAAGAAAACATGCATCACGCGCGTAATCAAAAAGGAATGAAGCTCCCATCCAAAAGACACATTGCGACACAGCATTCCATAAGTCAAACGACTGTAGAACGTCCCTATTAACAGCTAGCTGCTGAGTCCTATATc                                                                   >  2:3335127/4‑140 (MQ=255)
                                                                        aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAATCGACTGTAGAACGTGCCTTTGAACAGCTTGCTGCTGAGGGCTATATc                                                                   >  1:7605852/1‑140 (MQ=255)
                                                                        aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGGACAGCTTGCTGCTGAGGGCTATATc                                                                   >  1:7005776/1‑140 (MQ=255)
                                                                        aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTCTATc                                                                   >  1:1097405/1‑140 (MQ=255)
                                                                        aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATc                                                                   >  1:5934253/1‑140 (MQ=255)
                                                                        aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATc                                                                   >  1:6669961/1‑140 (MQ=255)
                                                                        aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATc                                                                   >  1:1029427/1‑140 (MQ=255)
                                                                        aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACACCTTGCTGCTGAGGGCTATATc                                                                   >  2:6046913/1‑140 (MQ=255)
                                                                         tttCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCg                                                                  >  1:2309299/1‑140 (MQ=255)
                                                                              aataaaaaaTGAATCTGGGGCTTATAAAAAAAGGAATCAAGCCCCCTTCCAAAACACTCCTGGCGAGACAGCTTTCCATCAGTAACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGc                                                             <  1:4420508/137‑1 (MQ=255)
                                                                              aaGAAAACAGGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCAAAAAGACTCCGGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGc                                                             <  2:5912812/140‑1 (MQ=255)
                                                                                   aaCATGCATCAGGGGCGTATTAAAAAAGTAAAGAATATCCATTAAAAAACACTCCTGGCGAGTAAGCTTTCCATCATTCAAACGACTGTAGAACGTGCCTATGAACGGCTTGCTGCTGAGGGCTATAACGTGAGAAAGcc                                                        >  2:4253703/1‑140 (MQ=255)
                                                                                      aggaaccAGGGGCGTATTCAAACAGGAACTAAGCTCCCTTCCAACCGACGCCGGGCGAGTCAGCTTTCCAACAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCaag                                                     <  2:4532082/134‑1 (MQ=255)
                                                                                            ccggggCTTATAAAAAAAGGAATGAAGCTCCCTTCCAAAAGACGCGTGGCGAGTCAGCTTTCCATAAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCgg                                               <  1:7155487/138‑1 (MQ=255)
                                                                                             aGGGGCGTATTCAAAAAGTAATTAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCATACAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGa                                              <  2:5157259/140‑1 (MQ=255)
                                                                                               gggCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATg                                            >  1:5262021/1‑140 (MQ=255)
                                                                                                  cGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGtt                                         >  2:474353/1‑140 (MQ=255)
                                                                                                    tataaaaaaaGCATGGAAGCTCCCTTCCAAAACACTCCGGGCGAGTCAGCTTTCCAACAGACACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                                       <  1:3721806/135‑1 (MQ=255)
                                                                                                    tataCAAAAAGGAATGAAGCCCCCTTCCAACACACTCCTGGCGAGTCAGCTTCCCATCACACACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                                       <  1:6492555/140‑1 (MQ=255)
                                                                                                    tATTCAAAAAGGAATTAAGCTCCATTCCAAAAGACTCGTGGCTAGTCAGATTTCCATCAGACAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                                       <  2:5641526/140‑1 (MQ=255)
                                                                                                    tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCa                                                                                                                           <  2:7266053/56‑1 (MQ=255)
                                                                                                    tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                                       >  1:3995540/1‑140 (MQ=255)
                                                                                                    tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                                       >  1:5153019/1‑140 (MQ=255)
                                                                                                      ttCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCt                                     >  2:7187478/1‑140 (MQ=255)
                                                                                                      ttCAAAAAGGAAGGAAGCTCCCTTCCAACAGACGCCTGGCGAGTCAGCTTTCCATCCTCCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCt                                     <  2:6907785/140‑1 (MQ=255)
                                                                                                         aaaaaGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAGCGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGc                                                             <  1:7292250/113‑1 (MQ=255)
                                                                                                           aaaGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGCTGGTTTGCTGATTa                                >  2:4924727/1‑140 (MQ=255)
                                                                                                                         cccTTCCAAAAGACTCCTGGCGAGTCAACTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCGGATTATCATGATTCTGAtt                  >  2:7535230/1‑140 (MQ=255)
                                                                                                                          ccTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGAttt                 >  2:3701663/1‑140 (MQ=255)
                                                                                                                          ccTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGAttt                 >  1:2835501/1‑140 (MQ=255)
                                                                                                                          ccTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGAttt                 >  1:675328/1‑140 (MQ=255)
                                                                                                                            ttCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTg               >  2:4367898/1‑140 (MQ=255)
                                                                                                                                aaaccccTCCGGGCGCGGAAGCTGTCCATCAGGCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTa           <  1:4572739/134‑1 (MQ=255)
                                                                                                                                aaaaGACTCCTGGCGAGTCAGCTTCCAATCAGACACACGACTGGAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTa           <  1:1936555/140‑1 (MQ=255)
                                                                                                                                aaaaCACTCCTGGCGATTCATCTTTCAATCATTAAATCGACTGTAGAACGTGCCTATGAACAGCTTGCTTCTGATGGATATATCTTTAGCAATAAAATAATAGGATTGTTTTATTATTATCATGATTCTGAtttttctta           <  2:6309547/140‑6 (MQ=255)
                                                                                                                                 aaaGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTAt          >  1:7386754/1‑140 (MQ=255)
                                                                                                                                       tCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTaaa    >  2:5446311/1‑140 (MQ=255)
                                                                                                                                       tCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTaaa    >  1:2426213/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                          
CAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAAT  >  NZ_CP034943/548981‑549257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑