BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,430	G→C	5.4%	A91A (GCG→GCC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,430	0	G	C	5.4%	741.4 / 8.6	303	A91A (GCG→GCC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base G (88/192); new base C (2/20); total (91/212)
Fisher's exact test for biased strand distribution p-value = 2.87e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 375 total aligned reads displayed.

GGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGG  >  NZ_CP034943/607292‑607569

ggAAGCTGGACCAGCCGGAGGGACGGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcga                                                                                                                                            <  1:4373278/140‑1 (MQ=255)

 gAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                                                                                           <  2:569701/140‑1 (MQ=255)

    gCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAGCCGGACCACGCGGACGCACGGGCGCGAGGGGAGCTAGCTCGGAAGCGGGACCAGCCGGACCACCCGGcgcgccaggcgcgccagg                                                                                                                                        >  2:4106071/1‑140 (MQ=12)

    gCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGagcgacaggagcgacagg                                                                                                                                        >  2:4612923/1‑140 (MQ=255)

    gCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGagcgacaggagcgacagg                                                                                                                                        >  2:696422/1‑140 (MQ=255)

    ccgggACCAGCCGGCGCGACGGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGagcgacaggagcgacagg                                                                                                                                        <  2:2476696/137‑1 (MQ=255)

         accacacGGAGCGACGGGAGCCACTGGAGCCACCGGCGCTCCCGGCCAAGCCGGACCACCCGGCGCGACTGGAGCCACGGGAGCTACGGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTa                                                                                                                                   <  1:1633394/134‑1 (MQ=14)

         aCCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTa                                                                                                                                   <  1:3573040/140‑1 (MQ=255)

              cctgAGCGACGCGAGCGACATGAGCTACTGGGGAAGCCGGACCAGCCGGACCAACCTTAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                                                                              <  2:3528262/137‑1 (MQ=17)

              ccGGAGCGACGGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                                                                              <  2:1251733/140‑1 (MQ=255)

                  aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                                                                                          >  1:1488623/1‑140 (MQ=255)

                  aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                                                                                          <  2:5886271/140‑1 (MQ=255)

                  aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                                                                                          <  1:696422/140‑1 (MQ=255)

                       cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                                     >  1:7711764/1‑140 (MQ=255)

                       cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGACGGACCAAACGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                                     >  1:3878346/1‑140 (MQ=255)

                        gggCGCGACCGGACCTACCGGCGAACCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                                    <  2:4992499/140‑1 (MQ=17)

                             cGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGACGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                               <  1:2593116/140‑1 (MQ=255)

                               actggAGCTACGGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                             <  2:2089252/137‑1 (MQ=255)

                               aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCGACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                             >  2:1972250/1‑140 (MQ=255)

                               aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                             >  2:5422816/1‑140 (MQ=255)

                               aCAGGAGCTACCGGCGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                             <  1:3090161/140‑1 (MQ=255)

                               aCAGGAGCGACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACGGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCgg                                                                                                             <  2:428699/140‑1 (MQ=255)

                                    aGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcga                                                                                                        <  1:4721546/140‑1 (MQ=255)

                                    aGCGACCGGCGACCCCCGCCCAGCCCGACCATCAGGAGCCACCGGAGCCACGGGAGCGACCGGGGATGCCGGACCAGCCGCACCATCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcga                                                                                                        <  2:516510/140‑1 (MQ=14)

                                     gCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgac                                                                                                       <  1:520151/140‑1 (MQ=255)

                                      cgaCCGGCGAATCCGGACCAGCCGGACGAGCCGGAGCGCAGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCACCCGGACCTCCCGGAGCGACCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgaca                                                                                                      <  2:3792386/138‑1 (MQ=17)

                                        aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacagg                                                                                                    >  2:6812684/1‑140 (MQ=255)

                                        aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGAGCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacagg                                                                                                    <  2:6209131/140‑1 (MQ=255)

                                        aCCGGGGAAGCCGGACCAGCCGCACCAACCGGCGCGACGGGAGCCACGGGAGCTACCGGGGACGCCGGACCAGCCGGACCAACGGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacagg                                                                                                    <  1:785236/140‑1 (MQ=255)

                                             ggccgccggacccgccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcga                                                                                               <  2:183784/136‑1 (MQ=255)

                                              gAAGCGGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgac                                                                                              <  1:1398994/140‑1 (MQ=255)

                                              gAAGCCGGACCAGCCGGACCAACAGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCAGGACCAGCCGTACCAACCGGAGCGACAGGAGCTACAGGACCAACCGGGGACGCCGCACCACCCgcagcgacaggagccaa                                                                                              >  1:5473160/1‑137 (MQ=17)

                                               aaGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgaca                                                                                             <  1:6056637/140‑1 (MQ=255)

                                                 gccggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagg                                                                                           >  1:3970349/1‑140 (MQ=255)

                                                   cggagccgccggaccaACCGGCGCGACTGCAGCCCCGGGAGCTCCCGGGGAAGCGGGACACTCCGGACAAACCGGAGCGACTGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggag                                                                                         <  1:4167948/140‑1 (MQ=17)

                                                   cggaccagccggaccaCCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCAGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggag                                                                                         <  1:6296450/140‑1 (MQ=255)

                                                   cggaccagccggaccaACCGGAGCGACGGGAGCCACGGGCGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggag                                                                                         <  1:7570123/140‑1 (MQ=255)

                                                   cggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggag                                                                                         >  1:1114781/1‑140 (MQ=255)

                                                   cggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggag                                                                                         >  1:1806194/1‑140 (MQ=255)

                                                   cggaccagccggaccaACCGGAGCGACGGGAGCCACCGGAGCTACCGGGGAAGCCGGACCACCCGGACCAACCGGAGCGACAGGAGCGCCAGGAGCTACCGGGGAAGCAGGACCAGCCggagggacaggtgcgacaggag                                                                                         >  1:5846723/1‑140 (MQ=14)

                                                    ggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgaccggagcgacaggagc                                                                                        >  2:4828696/1‑140 (MQ=255)

                                                      accagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcga                                                                                      <  2:5957530/140‑1 (MQ=255)

                                                       cccgccggtccaCCCGGAGCGAGGGGAGCCACGGGAGCTCACGGGGAAGCCGGACGAGGCGGACAAACGGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggcgcgacaggagcgacaggagcgac                                                                                     <  2:6767814/137‑1 (MQ=17)

                                                       ccagccggaccaCCCGGAGCGCCGGGAGCCACGGGAGCCACCGGGGAACCCGGCCCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac                                                                                     <  2:5347672/140‑1 (MQ=17)

                                                       ccagccggaccaCCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac                                                                                     <  1:659848/140‑1 (MQ=255)

                                                          gccggacccAGCGTCGGGACGGGAGCCTCGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgacagg                                                                                  <  2:6342559/140‑1 (MQ=17)

                                                          gccggaccaCGGGGAGCGACTGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACTGGAGCGACAGGAGCTACCGTGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgactgg                                                                                  <  2:7747705/140‑1 (MQ=17)

                                                          gccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACGGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgacagg                                                                                  <  2:7318391/140‑1 (MQ=255)

                                                          gccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                                                                                           >  2:1480625/1‑83 (MQ=255)

                                                           ccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAgcgacaggagcgaccggcgcgaccgga                                                                                 >  1:1258742/1‑140 (MQ=255)

                                                            cggacctCCCGGAGCGCCGGGAGCCACGGGAGCTACCGGTGAAGCCGGACCAGCCGGACCAACCGGAGCGACTGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGcgacaggagcgacaggagcgacaggag                                                                                <  1:5066182/140‑1 (MQ=14)

                                                                   aCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                                    <  1:2321658/97‑1 (MQ=255)

                                                                   aCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                                    >  2:2321658/1‑97 (MQ=255)

                                                                   aCCGGACCGACGGGAGGCACTGGAGCTACGGGGGAAGCCGGCCCCGCCGGACCATCCGGATCTACAGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGTGCGACAGGAGCGACAGGAGCGACAGGAGCTACCgg                                                                         <  1:3425317/140‑1 (MQ=14)

                                                                    ccGGAGCGACGGGAGCCACGGGCGCTCCCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGACTGGAGCGACAGGAGCTACCggg                                                                        <  2:7581298/140‑1 (MQ=17)

                                                                    ccGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                        <  1:1832753/140‑1 (MQ=255)

                                                                        aGCGACTGGAGCAACGGGAGCGACCGTGTGAGCCGGAGCATCCGGACCAACCGGAGCGACAGTGTCGACAGGAGATACCGGGGCCGCCGGACCAGCTGGAGCGACAGGAGCGTCAGGAGCGCCAGGACCTACCGGGGAAg                                                                    <  2:1678481/140‑1 (MQ=9)

                                                                        aGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                                    <  1:7732160/140‑1 (MQ=255)

                                                                             ccggAGCCAGGGGAGCGACAGGGGCAGCCGGACAAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGa                                                               <  2:5562713/138‑1 (MQ=17)

                                                                             cGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTggc                                                               >  2:918721/1‑139 (MQ=255)

                                                                             cGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGa                                                               >  2:399513/1‑140 (MQ=255)

                                                                             cGGGAGCCACGGGAGCGACCGGGGAAGCCGCCCCAGCCGGACCAACCGGAGCGACAGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGa                                                               <  1:2509909/140‑1 (MQ=14)

                                                                              gggAGCCACGGGCGCGACCGGGGAAGCCGGACCAGCCGGACCCCCCGGAGCGACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCTGGAc                                                              <  2:6148379/140‑1 (MQ=17)

                                                                              gggAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAc                                                              <  1:479792/140‑1 (MQ=255)

                                                                                  gCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACCGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGAGCGACCGGAGCTACCGGGGCCGCTGGACCAGc                                                          >  2:2953505/1‑140 (MQ=17)

                                                                                  gCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGATACCGGGCAAGCGGGACCAGc                                                          >  2:266060/1‑140 (MQ=255)

                                                                                   ccccGGGAGCTACCGGCGAAGCCGCACCAGCCGGACCAAGCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGACCGACAGGTGCGACAGGAGCGTCAGGAGCTACCGGGGAAGCTGGACCAGCt                                                         <  2:6563638/137‑1 (MQ=21)

                                                                                   ccACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGTAGCGACGGGTGCGGCAGGAGCTACCGGGGAAGCTGGACCAGCt                                                         >  2:3841069/1‑140 (MQ=255)

                                                                                   ccACGGGAGCGACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTTCCGGGGAAGCTGGACCAGCt                                                         <  1:6082571/140‑1 (MQ=255)

                                                                                              acggggtAAGCCGGACCAGCCGGACCACCCGGAGCCCCAGCAGCCACTGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                              <  1:1029910/137‑1 (MQ=14)

                                                                                              aCCGGCGCACCCGGACCAGCCGCACCAACCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCCACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                              <  2:354758/140‑1 (MQ=14)

                                                                                                cGGGGACGCCGGACCCGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCGACAGGAGCGCCAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAg                                            <  1:4106071/140‑1 (MQ=255)

                                                                                                cGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAg                                            <  1:4612923/140‑1 (MQ=255)

                                                                                                cGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAg                                            <  1:4654122/140‑1 (MQ=255)

                                                                                                    gctcccggaccagccggcccaCCCGGAGCGACCGGCGCCACGGGAGCTACCGGGGAAGCCGGCCCAGCCGGAGCCCCCGGAGCGACTGGAGCGACCGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                                        <  1:569229/136‑1 (MQ=11)

                                                                                                     aaGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgaca                                                                                                      <  1:6154350/77‑1 (MQ=255)

                                                                                                       gccggaccagccgtacaaACCGGAGAGACAGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCTCCAGCCGCGACAGGAGCGACAGGAGCTCCCGGGGAAGCTGGACCTGCTGGAGCGACGGGAGCGACAgg                                     <  1:1949476/140‑1 (MQ=17)

                                                                                                       gccggaccagccggaccaCCCGGAGCCACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGG                                     <  1:7443308‑M1/140‑1 (MQ=255)

                                                                                                       cccggaccagccggagcacCCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGG                                     <  2:1074258‑M1/139‑1 (MQ=255)

                                                                                                        ccggacctggcggaccaCCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCCACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGA                                    <  2:3337738‑M1/140‑1 (MQ=255)

                                                                                                           gaccagccggaccaACCGGAGCGACAGGAGCGACAGGATCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCt                                 <  2:4368524/140‑1 (MQ=255)

                                                                                                            accagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGCGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTa                                <  1:6812684/140‑1 (MQ=255)

                                                                                                            accagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTa                                >  2:6286162/1‑140 (MQ=255)

                                                                                                                gccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac                                                                                     <  2:4470161/83‑1 (MQ=255)

                                                                                                                 ccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCggg                           >  1:1681821/1‑140 (MQ=255)

                                                                                                                 ccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACCGGAGCGACAGGGGCTACAGGCGCTCGCGGAGACGCAGGACCTCCCCGAGCGCCCCGGGCCACAGGAGGTAGCggg                           >  1:5330426/1‑140 (MQ=9)

                                                                                                                     accaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAg                       <  1:4515887/140‑1 (MQ=255)

                                                                                                                         aCCGGAGCGACAGGAGCGACAGGGGAATCCGGCGAACCCGGACCACCCGGAGCGACAGGAGCGACTGGGGCGCCCGGAGCTGCCGGCGACGCTGGAGCGCCGGGGGCGGCGGGAGCGAGAGGAGCGGCCGGCGacggggg                   >  1:7248129/1‑134 (MQ=255)

                                                                                                                          ccGGAGCGACAGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGa                  <  1:6763548/140‑1 (MQ=255)

                                                                                                                          ccGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGa                  <  1:4904385/140‑1 (MQ=255)

                                                                                                                          ccGGAGCGAAAGGAGCGACTGGAGGTCCCGGTGACGCCGGACAAGCGTTACCGACAGGCCCGACAGGAGCTACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGAGGGGAGCGACAGGAGCTACCGGGGAAGCCGGa                  <  1:2803161/140‑1 (MQ=17)

                                                                                                                           cGGAGCGACCCGAGCGACAGGCGCTACCGGGGAAGCCCGACCAGCCGGAGCGACAGGACCGCCAGGAGCGACAGGACCTCCCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                 <  1:2745656/140‑1 (MQ=18)

                                                                                                                                cgaccggagcgcccggagCTACCGGGGAAGCCGGACCAGCCGGAGCGCCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc            <  2:521349/140‑1 (MQ=17)

                                                                                                                                cgacaggagcgacaggcgCGCCCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc            <  1:651065/140‑1 (MQ=255)

                                                                                                                                cgacaggagcgacaggagCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc            <  1:7152778/140‑1 (MQ=255)

                                                                                                                                   caggagcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggg         >  2:5263376/1‑140 (MQ=255)

                                                                                                                                        gcgacaggagcTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagg                                                                                           <  2:7652905/53‑1 (MQ=255)

                                                                                                                                        gcgacaggagcTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagg                                                                                           >  1:7652905/1‑53 (MQ=255)

                                                                                                                                         cgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTGCAGCGGAAGCTGGACCAGCTGGAGCGAAGGGAGCGCCCGGACCGCCCGGGGCCGCCGGACCAGCGGGAGacacc   >  1:3477080/1‑139 (MQ=17)

                                                                                                                                         cgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGGGCCACg   >  1:6449566/1‑140 (MQ=255)

GGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGG  >  NZ_CP034943/607292‑607569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`