Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,562 G→C 9.0% G135G (GGG→GGC EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,5620GC 9.0% 655.7 / 7.6 281G135G (GGG→GGCEO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base G (116/125);  new base C (11/13);  total (137/144)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

CAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGA  >  NZ_CP034943/607423‑607699
                                                                                                                                           |                                                                                                                                         
  ggagcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCCGGGAAGCTCGACAACCACTCTCAACCGGACCCACACGCGCTACCGGGGAAGCCGGCGCAGCCGGGGc                                                                                                                                         >  2:4197805/1‑140 (MQ=17)
     gcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCAc                                                                                                                                      <  1:6379423/140‑1 (MQ=255)
      cgacaggcgcTACCGGGGAACCGGGACCACCCGGAGCGACAGGCGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGGGCCACg                                                                                                                                     <  2:400193/140‑1 (MQ=18)
       gacaggagcTACCGGGGATGCCGGACCAGCCGGAGCTACAGGAGCGACTTGAGCGACAGGAGCAACCGGGGTAGGTGGACCAGCTGGAGCGACGGGCGCGACAGGAGCTACCGGGGAAGCCGGACGAGCCGGGGCCACgg                                                                                                                                    <  2:6741040/140‑1 (MQ=18)
       caccggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACgg                                                                                                                                    <  2:4465251/136‑1 (MQ=255)
        ccaggagcTACCGGGGAAGCCGGACGAGCCGGAGCGAGCGGAGCGACAGGAGCGACAGGAGATGCCGGGGAAGCTGGACCCGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                                   <  1:5342303/139‑1 (MQ=18)
        acaggagcTACGGGGGAAGCCGGACCCGCCGGAGCGACAGGCGCGACTGGAGCGACAGGAGCTACGGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                                   <  1:5077106/140‑1 (MQ=18)
        acaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGCAGCGGGACCAGCTGGAGCGACGGGAGCGAAAGGAGCTACCGGAGACACAGGCCCAACCGGAGCCACggg                                                                                                                                   >  2:7687485/1‑140 (MQ=17)
        acaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                                   <  1:5168873/140‑1 (MQ=255)
        acaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCCACAGGAGCGACAGGAGCAGGCGCAGAAGCGGGAGCAGCAGGCGCGACGGGAGCAACAGGAGCTACCGGGGACGCCGGACCAACCGGTGCCACtgg                                                                                                                                   >  2:4579761/1‑140 (MQ=17)
        acaggagcTACCGGGGAAGCCGCACCACCCGGCGCGACAGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                                   <  1:861321/140‑1 (MQ=255)
        acaggagcTACCGGCGAACCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                                   <  1:973602/140‑1 (MQ=255)
          aggagcTACCGGGGAAGCCGGACCAGCCGGCGCGACCGGAGCGACAGGCGCGACAGGAGCTACCGGGGAAGCAGGAGCCGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGGGCCCCGGGAg                                                                                                                                 >  2:2922349/1‑140 (MQ=17)
              gcTCCCGGGGAAGCCGGACCAGCCGGTGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                                                                                             <  1:4880319/140‑1 (MQ=255)
              gcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                                                                                             >  2:3699173/1‑140 (MQ=255)
               gtACCGGGGAAGCCGGAACAGCCGGAGCGACAGGAGCCACAGGAGCCACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                                                                            <  1:6060119/139‑1 (MQ=255)
               cttccGGGTAAGCCGGACCAGCCGGAGCGACAGGAGCCACAGGAGCGACAGGAGCTCCCGGGGAAGCGGGAGCCGCTGGAGCGACGGGAGCGACAGGCGCTCCCGGGGAAGCCGGACCAGCCGGCGCCACGGGAGCcaca                                                                                                                            <  1:4623918/137‑1 (MQ=18)
               cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGTGCGACAGGAGCACACGGCCAAGCGGGACCATCAGGAGCTACGGGAGCGACAGGACCTACAGGCGAAGCCCGACCTCCCGGGGCCACGGGAGCcacc                                                                                                                            >  1:1289282/1‑139 (MQ=18)
                tACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGCGAAGCCGGACCCGCCGGGGCCCCGGGAGCcaccg                                                                                                                           >  1:1686830/1‑138 (MQ=255)
                tACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAg                                                                                                                           >  1:4426687/1‑140 (MQ=255)
                 aCCGGGGCAGCCGGCCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                                                                          <  1:3812043/140‑1 (MQ=255)
                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                                                                          <  2:6148848/140‑1 (MQ=255)
                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                                                                          <  2:1681821/140‑1 (MQ=255)
                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                                                                          <  1:399513/140‑1 (MQ=255)
                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                                                                          <  1:2041683/140‑1 (MQ=255)
                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                                                                          <  2:114048/140‑1 (MQ=255)
                 aCCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                                                                          <  1:6652621/140‑1 (MQ=255)
                      ggAAGCCGGACCAGCCGGAGCGACCGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                                                                     <  1:1972250/140‑1 (MQ=255)
                      ggAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTAGCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAGAGGAGCTCCCGGGGCAGCCGGACCTGCGGGGGCGACGGGCGCCCCAGGGGCtc                                                                                                                     >  1:4103401/1‑139 (MQ=18)
                      ggAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                                                                     <  1:6286162/140‑1 (MQ=255)
                       gAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGAGCGACAGGAGCTACCGGGGACGCTGGCCCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTAc                                                                                                                    >  1:6462694/1‑140 (MQ=255)
                       gAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                                                                            <  2:210477/132‑1 (MQ=255)
                       gAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCtcc                                                                                                                    >  1:3096726/1‑138 (MQ=255)
                           ccGGACCAGCCGGAGCGACAGGAGCCACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTggg                                                                                                                <  1:4374746/140‑1 (MQ=255)
                            cGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGACGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGCGCCACGGGCGCCACAGGGGCTACGgggg                                                                                                               >  2:1549716/1‑140 (MQ=18)
                                  aGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCg                                                                                                         >  1:1018063/1‑140 (MQ=255)
                                   gCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCCACAGGAGCTACCGGGGACGCCGGACCACCCGGGGCCACGGGAGCCCCAGGGGCTACGGGGGAAGGCgg                                                                                                        >  2:2585140/1‑140 (MQ=21)
                                     cGGAGCCCCAGGAGCGACAGGAGCGACAGGAGCGACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGAc                                                                                                      <  2:4707720/140‑1 (MQ=255)
                                        agcgacaggagcgacaggagcgacaggAGCTACCGGGGACGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCAACAGGGCCTACGCGGGAAGCCGGACCAg                                                                                                   >  1:5609210/1‑140 (MQ=21)
                                        agcgacaggagcgacaggagcgacaggAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAg                                                                                                   >  1:1163429/1‑140 (MQ=255)
                                        agcgacaggagcgacaggagcgacaggAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACAGGGGAAGCCGGACCAg                                                                                                   >  2:3871525/1‑140 (MQ=255)
                                            cccggagcgactggagcgacaggtgcTACAGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCgg                                                                                               <  2:2838465/137‑1 (MQ=255)
                                              aggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAg                                                                                             >  1:4226909/1‑140 (MQ=255)
                                              aggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAg                                                                                             >  1:5268368/1‑140 (MQ=255)
                                                  gcgccaggaccgacaggaccTACCGGCGCCGCTGGACCCCCGGGAGAGCCGGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGGGCCACGGGAGCCACCGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                         <  2:3072920/140‑1 (MQ=18)
                                                  gcgacaggagcgacaggcgcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                         <  1:2359061/140‑1 (MQ=255)
                                                   cgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACa                                                                                        >  2:1990340/1‑140 (MQ=255)
                                                     tctcgcgcgacaggtgcGCCCGGGGCAGCTGCACCAGCTGGAGCGACGGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGTGCCACGGGAGCCACAGGGGATACTGGTGAAGCCGGACCAGCCGGAGCGACAgg                                                                                      <  1:4065436/134‑1 (MQ=18)
                                                     acaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                      >  2:2096223/1‑140 (MQ=255)
                                                       aggagccacagcagcTCCCGGGCAAGCGGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGTGCCACGGGTGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                                                    <  2:1258742/140‑1 (MQ=21)
                                                          agcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGAAACAGGGGCTACTGGGGAAGCCTGACCAGCCGGAGCGACAGGAGCTa                                                                                 >  1:596025/1‑140 (MQ=255)
                                                            cgacaggagcTACCGGGGACGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAg                                                                                                            <  2:5057858/111‑1 (MQ=255)
                                                               caggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                            <  1:3699173/140‑1 (MQ=255)
                                                               cacgcgcTACCGGGGAAGCTGGACCACCTGGAGCGACGGCAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                            <  1:2745784/135‑1 (MQ=255)
                                                                   agcTACCGGGGACGCTGGACCCGCTGGAGCGACGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                        <  2:5268368/140‑1 (MQ=255)
                                                                   agcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                        <  1:455139/140‑1 (MQ=255)
                                                                     cTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCCCAGGGGCTACTGGGGAAGCCGGACCAGCCGGACCGCCAGGAGCTCCCGGGGAAGcc                                                                      >  2:4543245/1‑140 (MQ=255)
                                                                     cTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGcc                                                                      >  1:6911451/1‑140 (MQ=255)
                                                                     cTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGcc                                                                      >  2:2597622/1‑140 (MQ=255)
                                                                      tACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                      <  1:7655136/123‑1 (MQ=255)
                                                                       aCCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGTGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                                                                    >  1:7407362/1‑140 (MQ=255)
                                                                       aCCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCgg                                                                    >  2:5774981/1‑140 (MQ=255)
                                                                       aCCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                                                                    >  1:3164677/1‑140 (MQ=255)
                                                                       aCCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCGACGGGAGCCACAGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCCGCCgg                                                                    >  1:7005253/1‑140 (MQ=18)
                                                                            ggAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGAAGACGGACCTGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAccgg                                                               >  2:5385183/1‑138 (MQ=18)
                                                                             gAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCGGGCGACGGCGGACCCGCCGGAGCGACAGGAGCTCGCGGGGACGCCGGACCAGc                                                              >  2:5098274/1‑140 (MQ=18)
                                                                             gAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACGCGAGCCCCCGGGGCGACAGGGCACGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGACGCCGGACCGGc                                                              >  2:4080576/1‑140 (MQ=14)
                                                                                gCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                           <  2:3164677/140‑1 (MQ=255)
                                                                                gCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                           <  1:2596811/140‑1 (MQ=255)
                                                                                      cgcgCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                     <  1:6199674/137‑1 (MQ=255)
                                                                                          cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa                                                 >  1:6972329/1‑140 (MQ=255)
                                                                                          cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa                                                 <  1:5696182/140‑1 (MQ=255)
                                                                                          cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCGCCGGGAGCCACCGGGGCTACTGGGGAAGGCGGACCAGCCGGCGCGACAGGAGCTACCGGGGAAGCCGGACCTCCCGGAGCGCCAGGa                                                 >  2:731999/1‑140 (MQ=14)
                                                                                              cgccaCGGGAGCCACCGGCGCTACCGGCGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGCCAACTGGGGAAGCCGGACCAGCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                             <  1:2274173/136‑1 (MQ=17)
                                                                                              agctcccggAGCCACAGGCGCTACCGGCGACGCCGGACCACCCGCGGCCACGGGAGCCACAGGGCCTCCGGGTCAAGCGGGACCAGACGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAGCCGGAGCGACAGGAGCTa                                             <  1:7762266/133‑1 (MQ=14)
                                                                                              aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGa                                                                                          >  1:3280283/1‑95 (MQ=255)
                                                                                              aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                             <  1:5774981/140‑1 (MQ=255)
                                                                                              aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCACCAGGCGAAGGCGGAGCCGCCGGCGCGACAGGAGCTACCTGGCAAGCCCGAACAGCCGCAGGGACAGGAGCTa                                             >  2:2222456/1‑140 (MQ=17)
                                                                                                cGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGCAATCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCCGACAAGCCGGAGCGACAGGAGCAAcc                                           >  2:7434327/1‑140 (MQ=17)
                                                                                                  aCGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                         <  2:2791101/140‑1 (MQ=255)
                                                                                                  aCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                         >  2:4937940/1‑140 (MQ=255)
                                                                                                  aCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCAACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTCCCgg                                         >  2:3309802/1‑140 (MQ=255)
                                                                                                   cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                                   >  1:3496184/1‑49 (MQ=255)
                                                                                                   cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                                   <  2:3496184/49‑1 (MQ=255)
                                                                                                   cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACCGGGGCTACTGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                        <  2:555243/140‑1 (MQ=255)
                                                                                                   cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGAAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                        >  1:672031/1‑140 (MQ=255)
                                                                                                           acccgAGCGACCGGGGACTCCGGACCAGCCGCCGCCCAGCGAGCCACAGGGGCTACTGGCGAACCCGGACCAGCCGGAGCCACAGGCGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                                <  2:6320423/136‑1 (MQ=14)
                                                                                                                  cTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                                                                                         >  1:4744924/1‑76 (MQ=255)
                                                                                                                    aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACTGGGGCTAAAGGGGAATCCGGACCAGCCGGAGCGACAGGACCAACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGAAGCACCCACCgg                       >  1:2201335/1‑140 (MQ=17)
                                                                                                                    aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGCAGCGGGAGCAACCgg                       >  1:3417981/1‑140 (MQ=17)
                                                                                                                          gAAGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACGGGGGCCCCCGGACCAGCCGGAGCGGCCGGAGCGCCCGGGGCGGCGGGACCAGCCGCAGCggc                 >  2:1346119/1‑138 (MQ=17)
                                                                                                                          ccccccGCACCAGCCGGCGCCCCCGGAGCCCCAGGACCAGCAGGAGACGCCGGACCACCGGCAGCCACAGGAGCTACCGGGCAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                 <  2:6634342/136‑1 (MQ=2)
                                                                                                                          caaGCCGGACCTGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGAc                 <  2:6932062/139‑1 (MQ=255)
                                                                                                                             gCCGGCACAGCCGGGCCCACGGGAGCCACAGGGGCTTCTGGGTATGCCGGACCACCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg              <  2:1806194/140‑1 (MQ=17)
                                                                                                                             gCCGGAGCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg              <  2:6337876/140‑1 (MQ=255)
                                                                                                                             gCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg              >  2:2706624/1‑140 (MQ=255)
                                                                                                                             cccGGCCCACCCGGCGCCCCCGGAGCCACCGGGGCTCCGGGGGCACCCGGACCACCCGGCGCCACCGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg              <  2:785087/139‑1 (MQ=14)
                                                                                                                               cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg            <  1:2953505/140‑1 (MQ=255)
                                                                                                                                  aCCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCTGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTa         <  2:4351153/140‑1 (MQ=255)
                                                                                                                                       ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGCCTGACCCGCCCGACCGACCGGAGCTACCGGGGCAGCCGGACCGACCGGAGCGCCAGGAGCGCCCggg    >  1:7518036/1‑140 (MQ=17)
                                                                                                                                           |                                                                                                                                         
CAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGA  >  NZ_CP034943/607423‑607699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: