BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,638	G→C	7.0%	A161P (GCC→CCC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,638	0	G	C	7.0%	645.6 / 8.5	287	A161P (GCC→CCC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base G (147/104); new base C (7/17); total (159/128)
Fisher's exact test for biased strand distribution p-value = 8.63e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 291 total aligned reads displayed.

GAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTAC  >  NZ_CP034943/607500‑607774

   gCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                                      <  1:2596811/140‑1 (MQ=255)

   gCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                                      <  1:6370884/140‑1 (MQ=255)

   gCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                                      <  1:3495768/140‑1 (MQ=255)

         cgcgCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                                <  1:6199674/137‑1 (MQ=255)

         ccAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                                <  1:4745656/140‑1 (MQ=255)

             cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa                                                                                                                            <  1:5696182/140‑1 (MQ=255)

             cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa                                                                                                                            >  1:2293391/1‑140 (MQ=255)

                 aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACagcagcaa                                                                                                                        >  1:3126290/1‑138 (MQ=255)

                     accggAGCCACAGGACCCACCGCCCAACCCGCACCACCCGCCGCCACGGGAGCCACAGGGGCAACTGGGGAATCCCGACCAGCAGGAGCGACAGGAGCTACCGGGGAATCCGGACCACCCGGAGCGACAGGAGCTACCgg                                                                                                                    <  2:4156196/137‑1 (MQ=9)

                     aCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                                                    >  2:4937940/1‑140 (MQ=255)

                     aCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                                                    <  2:785123/140‑1 (MQ=255)

                      cGGGAGCGACAGGAGCTTCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGCGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                                   <  1:6979551/140‑1 (MQ=255)

                      cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACCGGGGCTACTGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                                   <  2:555243/140‑1 (MQ=255)

                       gggAGCGACCGGAGATACCGGGGCAGCCGGACCACCCGGCGCCACGGGAGCCACAGGCGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgggg                                                                                                                  <  2:7655607/140‑1 (MQ=14)

                         gAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGccacgggagcc                                                                                                                <  1:1990340‑M2/139‑5 (MQ=255)

                          aGCGACCGGAGCTCCCGGCGACGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGGCTACGGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                               <  1:4485029/140‑1 (MQ=17)

                             caCAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACCGGCGCACCAGGGGAAGCCGGACCAGCCGGAGCCACAGGAGCGACCGTGGAACCCGTACCACCCGGCGCAGCAGGCGCACCCggggcggcgg                                                                                                            >  2:6723308/2‑134 (MQ=9)

                               cAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                          <  2:3096726/140‑1 (MQ=255)

                                       aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                                  >  2:2684293/1‑140 (MQ=255)

                                       aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCgg                                                                                                  >  2:1860744/1‑140 (MQ=255)

                                       aCCGGGGAAGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGACGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCCACGGGGGCACCAGGACCagcagg                                                                                                  >  2:2767785/1‑135 (MQ=11)

                                             gAAGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACGGGGGCCCCCGGACCAGCCGGAGCGGCCGGAGCGCCCGGGGCGGCGGGACCAGCCGCAGCggc                                                                                            >  2:1346119/1‑138 (MQ=17)

                                                gCCGGAGCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                                                                         <  2:6337876/140‑1 (MQ=255)

                                                         gCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCAACCGGGGAACCCGGACCACCCGGAGCGCCAGGAGCGACCgg                                                                                >  2:214354/1‑140 (MQ=14)

                                                          ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCggg                                                                               >  1:7518088/1‑140 (MQ=255)

                                                          ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                                                               >  1:5790553/1‑140 (MQ=255)

                                                          ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                                                               <  1:6694291/140‑1 (MQ=255)

                                                               gCCCCGGGCGCCCCAGGTGCTCCTGGGGATCCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCGGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGc                                                                          <  2:1253271/140‑1 (MQ=11)

                                                                   cGGGAGCCACAGGGGCTAGTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGGCGGAGCGACAGGAGCTACCGGGGAAGCCggc                                                                      >  1:801250/1‑139 (MQ=255)

                                                                    gggATCCACAGGCGCTACTGGGTCAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                     <  2:6811156/140‑1 (MQ=11)

                                                                    gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGGCAGGAGCAACCGGGGCCGCCggcg                                                                     >  2:2409944/1‑138 (MQ=11)

                                                                    gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                     >  1:5312208/1‑140 (MQ=255)

                                                                    gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                     >  1:3922388/1‑140 (MQ=255)

                                                                        gCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAACGGAGCGACCGGAGCAACCGGGGGAGCAGGACCAGc                                                                 >  1:3367334/1‑140 (MQ=14)

                                                                         ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                               >  1:6853631/1‑93 (MQ=255)

                                                                           ccaGCGGCGACGGGGCAAGCCGGCCCACCCGGAGCGCCAGGAGCGACCGGGGAAGCCGCACCAGCCGGAGCGACCGCAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                              <  2:4103401/139‑1 (MQ=14)

                                                                            caGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGa                                                             >  2:3476655/1‑140 (MQ=255)

                                                                                ggCTCCCGGGGAAGCCGGACCAGCCGGCGCGTCAGGTTCTAGCGGGGTCGCCGGTCGAGCCGGTGCGACCGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGa                                                         <  1:7192493/140‑1 (MQ=11)

                                                                                ggCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGa                                                         <  1:2684293/140‑1 (MQ=40)

                                                                                 gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc                                                                                                    <  2:4346603/96‑1 (MQ=37)

                                                                                 gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc                                                                                                    >  1:4346376/1‑96 (MQ=37)

                                                                                 gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                        <  2:6972329/140‑1 (MQ=40)

                                                                                 gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                        <  1:3841069/140‑1 (MQ=40)

                                                                                  cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGGAGCCGGAGCAGCCGGAGCGGCa                                                       >  2:4396241/1‑140 (MQ=14)

                                                                                  cTACTGGGGAAGCCGGACCAGCAGGAGCGACAGGAGCTACCTGGGAAGCAGGACCAGCCGGAGAGACAGGAGATAAAGGGGAAGACCGACAAACCGGAGCGCCAGGAGCTACCGGAGAATCCGGACACGCCGCAGCGAcc                                                       >  1:4061822/1‑139 (MQ=21)

                                                                                    aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                     >  2:6113563/1‑140 (MQ=255)

                                                                                        gggACGCCGGACCACCCGGAGCGACAGGAGCTACCGGCGCAGCCGGACCCGCCGGAGCGACAGGAGCTCACGGCGCCGCCGGACCAACCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCt                                                 <  1:2196841/140‑1 (MQ=14)

                                                                                          gAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACAGGGGAAGCCGGACCAGCCGGAGCGACAGCAGCtcc                                               >  2:7438154/1‑138 (MQ=255)

                                                                                          gAAGCCGGTCCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCTGCCGGAGCGACAGGAGGTAGGGGGGGAGCCGGACCAAGCGGAGCGCCAGGTGCTCCCGGGGCAGCCGGGCGAGAGGGAGCGAGAGGAGCTAc                                               >  2:3632714/1‑140 (MQ=14)

                                                                                           aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa                                                                                                 <  2:3858392/89‑1 (MQ=33)

                                                                                           aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTAACGGGGAAGCCGGAGCAGCCGGAGCGACAGGAGCTccc                                              >  1:955871/1‑140 (MQ=255)

                                                                                           aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                <  2:7402765/122‑1 (MQ=38)

                                                                                           aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                >  1:5467149/1‑122 (MQ=38)

                                                                                           aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                              >  2:1707905/1‑140 (MQ=255)

                                                                                           aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGACGGACCAACCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTAcc                                              >  2:2094923/1‑140 (MQ=255)

                                                                                           aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                              >  2:1113615/1‑140 (MQ=255)

                                                                                           aaGCCGGACCAACCGGAGCGACAGGAGCTACCCGGGAATCCGGACCAGCCGGAGCCACAGGAGCTACCGGGGAAGCCCGCCCAACCGGAGCGACCGGAGCAACCGGGGAAGCCGGAGCTGCCGGAGCGCCAGGAGCTccc                                              >  2:2073871/1‑140 (MQ=14)

                                                                                             gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAg                                                                           <  1:6723553/109‑1 (MQ=35)

                                                                                             gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGAGCGACCGGAGCTACCgg                                            >  2:4254078/1‑140 (MQ=255)

                                                                                             cccGGACCACCCGGAGCCCCAGGAGCTCCCGGCGACCCCGGAGCACCCGGAGCGACAGGAGCACCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                            <  1:4376731/139‑1 (MQ=14)

                                                                                              ccGGACCCGCCGGAGCGACCGGAGCTACGGGGGAAGCCGGACCCTCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                           <  2:1157353/140‑1 (MQ=14)

                                                                                               cGGTCCATCCGGAGCGACTGGATCTCCCGGGGAAGCGGGACCAGCCGGAGCGACTGGAGCTACCGGGGAAGCGGGACCAAGCGGAGCGACAGGAGCTACCGGTGAAGCCGGACCTGCCGGAGCGACAGGAGCTACCgggg                                          <  1:205132/140‑1 (MQ=14)

                                                                                               cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgggg                                          <  1:127254/140‑1 (MQ=255)

                                                                                                ggACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                        >  2:942695/1‑125 (MQ=38)

                                                                                                  acctcccGGAGCGACCGGAGCTTCCGGGGACGCCGGAGCACCAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                       <  1:5078321/135‑1 (MQ=14)

                                                                                                  aCCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGCTCGACAGGAGCTAGCGGGGATGCCGGACGTTGCGGTGAGAGTGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                       <  1:4580674/140‑1 (MQ=14)

                                                                                                   ccACCCGGAGATACAGGAGCTCCCGGGGACCCCGGACCAGCCGGAGCCCCAGGAGCTACCGGAGAAGCCGGACCAACCGGAGCGACAGGCGCTACCGGGGAAGCGGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGc                                      <  1:2532430/140‑1 (MQ=14)

                                                                                                      gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                                   >  1:1286269/1‑140 (MQ=255)

                                                                                                      gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                              >  2:6732857/1‑113 (MQ=39)

                                                                                                           aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCaa                              >  1:6603823/1‑140 (MQ=255)

                                                                                                                cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGa                         >  2:3220454/1‑140 (MQ=255)

                                                                                                                        aCCGGGGACGCCGTCCCAGCCGGAGCTACAGGCGCTACCGGTGAAGCCGGACCAACGGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                 <  2:2293391/140‑1 (MQ=14)

                                                                                                                        aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCgg                                                                                >  2:4069413/1‑77 (MQ=39)

                                                                                                                        aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCgg                                                                                <  1:145833/77‑1 (MQ=39)

                                                                                                                        aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCgg                                                                                >  2:145833/1‑77 (MQ=39)

                                                                                                                         ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGCAGCCGGACCAGCCGGAGCTCCCGGAGCGACCGGAGCCACCGGCCCAGCCGGAGCTCCGGGa                >  2:2888938/1‑140 (MQ=11)

                                                                                                                              gccgCCGGACCAGCCGGAGCGACAGGCGCTCCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAGCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac           <  2:6522067/137‑1 (MQ=255)

                                                                                                                              gacgCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac           <  2:4494857/137‑1 (MQ=255)

                                                                                                                              gAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAATCAGGACCAACCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCATCCGCAGCGACAGGAGCTACAGGCGAAACCGGACCACCCGGAGCGAAGGGAGCcac           >  2:5087430/1‑140 (MQ=11)

                                                                                                                              gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac           <  1:7415905/140‑1 (MQ=255)

                                                                                                                              gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac           <  2:5312208/140‑1 (MQ=255)

                                                                                                                              gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac           <  2:3922388/140‑1 (MQ=255)

                                                                                                                              gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACCGGCGCAGCAGGACCAGCCGGAGCGGCGGGAGCAACAGGGGCGGCAGGCCCAACCGGAGCGCCGGGTGCcac           >  2:1166951/1‑140 (MQ=14)

                                                                                                                              caacccGGACCCCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac           <  2:4804837/136‑1 (MQ=255)

                                                                                                                               aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca          <  1:5901535/140‑1 (MQ=255)

                                                                                                                                 gCCGGAGCCGCGGGAGCGACAGGCGCTCCCGGGGAAGCCCGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg        <  2:6911451/140‑1 (MQ=14)

                                                                                                                                 gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCg                                                                                 >  2:305948/1‑67 (MQ=35)

                                                                                                                                   cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCACCCGGAGCGACGGGAGCGACAgggg      >  2:5464629/1‑140 (MQ=255)

                                                                                                                                    ggACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGc     <  1:2759902/140‑1 (MQ=255)

                                                                                                                                      cccAGCCTGCGCGACCGCAGCGACCGGGGAAGCCGGACCAACCGGCGCGCCAGGAGCGACCGGGGAAGCCGCACCAGCCGGAGCCACCGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACTGGAGCCACAGGGGCTa   <  1:1643342/139‑1 (MQ=14)

                                                                                                                                      aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGCCGCCGGCCCAGCCGGAGCCGCAGGAGCGACCGGGGCCGCCGGAGCCACCGGGGCGCCGGGAGCCCCCGGGGcca   >  2:54019/1‑138 (MQ=14)

                                                                                                                                      aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGc                                                                          >  2:6657940/1‑69 (MQ=35)

                                                                                                                                      aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGc                                                                          >  2:5820955/1‑69 (MQ=35)

                                                                                                                                      aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGCACCCGGACCAACCGGAGCGACGGGAGCCACAGGGGcga   >  2:3956117/1‑138 (MQ=255)

                                                                                                                                      aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTa   >  2:7228967/1‑140 (MQ=255)

                                                                                                                                      aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGcga   >  2:1643342/1‑138 (MQ=255)

                                                                                                                                      aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTa   <  1:5196651/140‑1 (MQ=255)

                                                                                                                                      aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTa   >  2:5196651/1‑140 (MQ=255)

                                                                                                                                      aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAGCCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCGACCGGGGcga   >  2:4423127/1‑138 (MQ=11)

                                                                                                                                          gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTAc                                                                                   >  1:1442238/1‑56 (MQ=35)

GAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTAC  >  NZ_CP034943/607500‑607774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`