Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,688 A→G 8.7% G177G (GGA→GGG EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,6880AG 8.7% 738.9 / 55.5 305G177G (GGA→GGGEO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base A (154/115);  new base G (21/5);  total (176/129)
Fisher's exact test for biased strand distribution p-value = 2.14e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

Only 100 of 305 total aligned reads displayed.

CGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACC  >  NZ_CP034943/607550‑607825
                                                                                                                                          |                                                                                                                                         
cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                                                                                                                          <  1:2953505/140‑1 (MQ=255)
       gCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCgg                                                                                                                                   >  1:4472224/1‑140 (MQ=255)
                 cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                                         >  1:3136927/1‑140 (MQ=255)
                  gggATCCACAGGCGCTACTGGGTCAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                                        <  2:6811156/140‑1 (MQ=11)
                  gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                                        >  1:3922388/1‑140 (MQ=255)
                  gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                                        <  1:2706624/140‑1 (MQ=255)
                      gcgaccggggCTACGGGGGAAGCCGGAGCCGCCGGAGCGACAGGTGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                                                                                                                    <  2:1163429/134‑1 (MQ=11)
                          caGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGa                                                                                                                >  2:3476655/1‑140 (MQ=255)
                               gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                           <  2:6972329/140‑1 (MQ=40)
                               gCTACGGGGGAAGCGGGACCAGCCGGAGCGACAGGAGCTACCGGTGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                           <  1:3381442/140‑1 (MQ=255)
                                cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACa                                                                                                          >  2:4855992/1‑140 (MQ=40)
                                cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACa                                                                                                          >  2:1444590/1‑140 (MQ=40)
                                cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGGAGCCGGAGCAGCCGGAGCGGCa                                                                                                          >  2:4396241/1‑140 (MQ=14)
                                cTACTGGGGAAGCCGGACCAGCAGGAGCGACAGGAGCTACCTGGGAAGCAGGACCAGCCGGAGAGACAGGAGATAAAGGGGAAGACCGACAAACCGGAGCGCCAGGAGCTACCGGAGAATCCGGACACGCCGCAGCGAcc                                                                                                          >  1:4061822/1‑139 (MQ=21)
                                      gggACGCCGGACCACCCGGAGCGACAGGAGCTACCGGCGCAGCCGGACCCGCCGGAGCGACAGGAGCTCACGGCGCCGCCGGACCAACCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCt                                                                                                    <  1:2196841/140‑1 (MQ=14)
                                        gAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACAGGGGAAGCCGGACCAGCCGGAGCGACAGCAGCtcc                                                                                                  >  2:7438154/1‑138 (MQ=255)
                                         aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                                   >  1:7402765/1‑122 (MQ=38)
                                         aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                                                                                 >  2:927265/1‑140 (MQ=255)
                                         aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                                                                                 >  2:7575270/1‑140 (MQ=255)
                                         aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                                                                                 >  2:1113615/1‑140 (MQ=255)
                                         aaGCCGGACCAACCGGAGCGACAGGAGCTACCCGGGAATCCGGACCAGCCGGAGCCACAGGAGCTACCGGGGAAGCCCGCCCAACCGGAGCGACCGGAGCAACCGGGGAAGCCGGAGCTGCCGGAGCGCCAGGAGCTccc                                                                                                 >  2:2073871/1‑140 (MQ=14)
                                           gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGCCGCCGGACCAGCCGGAGCGCCTGGAGCTACCgg                                                                                               >  1:5308407/1‑140 (MQ=255)
                                           gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                                              <  1:6723553/109‑1 (MQ=35)
                                           gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                               >  2:4115359/1‑140 (MQ=255)
                                           gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGAGCGACCGGAGCTACCgg                                                                                               >  2:4254078/1‑140 (MQ=255)
                                           gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCAGCCGGACCAACCGGACCGACCGGAGCGACAGGGGATACCGGCCCAGCCGGAGCAACCGGAGCGACCgg                                                                                               >  2:21023/1‑140 (MQ=14)
                                            ccGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                              <  1:4855992/140‑1 (MQ=255)
                                             cGGTCCATCCGGAGCGACTGGATCTCCCGGGGAAGCGGGACCAGCCGGAGCGACTGGAGCTACCGGGGAAGCGGGACCAAGCGGAGCGACAGGAGCTACCGGTGAAGCCGGACCTGCCGGAGCGACAGGAGCTACCgggg                                                                                             <  1:205132/140‑1 (MQ=14)
                                             cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGCAGCGCCCGGAGCAACAggcg                                                                                             >  2:3082843/1‑138 (MQ=11)
                                              ggACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                           <  1:942695/125‑1 (MQ=38)
                                                acctcccGGAGCGACCGGAGCTTCCGGGGACGCCGGAGCACCAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                          <  1:5078321/135‑1 (MQ=14)
                                                aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                          <  1:441687/140‑1 (MQ=255)
                                                aCCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGCTCGACAGGAGCTAGCGGGGATGCCGGACGTTGCGGTGAGAGTGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                          <  1:4580674/140‑1 (MQ=14)
                                                 ccACCCGGAGATACAGGAGCTCCCGGGGACCCCGGACCAGCCGGAGCCCCAGGAGCTACCGGAGAAGCCGGACCAACCGGAGCGACAGGCGCTACCGGGGAAGCGGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGc                                                                                         <  1:2532430/140‑1 (MQ=14)
                                                    gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                                                                                      >  1:1286269/1‑140 (MQ=255)
                                                    gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGAAGCCgg                                                                                      >  1:5470493/1‑140 (MQ=255)
                                                    gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                 >  2:6732857/1‑113 (MQ=39)
                                                              cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGa                                                                            >  2:3220454/1‑140 (MQ=255)
                                                                    cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACg                                                                      <  1:1742167/140‑1 (MQ=40)
                                                                      aCCGGGGACGCCGTCCCAGCCGGAGCTACAGGCGCTACCGGTGAAGCCGGACCAACGGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                    <  2:2293391/140‑1 (MQ=14)
                                                                      aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCgg                                                                                                                                   >  2:7088598/1‑77 (MQ=39)
                                                                      aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCgg                                                                                                                                   <  1:7088598/77‑1 (MQ=39)
                                                                      aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCgg                                                                                                                                   <  1:145833/77‑1 (MQ=39)
                                                                      aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCgg                                                                                                                                   >  2:4069413/1‑77 (MQ=39)
                                                                      aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCAACCGGGGAAGCAGGAACAGCCGGAGCGGCCTGAGCTCCACGCGAAGCGGGTCCAACCGGAGCGACcgg                                                                    >  2:3806238/1‑140 (MQ=14)
                                                                       ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGCAGCCGGACCAGCCGGAGCTCCCGGAGCGACCGGAGCCACCGGCCCAGCCGGAGCTCCGGGa                                                                   >  2:2888938/1‑140 (MQ=11)
                                                                            gacgCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                              <  2:4494857/137‑1 (MQ=255)
                                                                            gAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAATCAGGACCAACCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCATCCGCAGCGACAGGAGCTACAGGCGAAACCGGACCACCCGGAGCGAAGGGAGCcac                                                              >  2:5087430/1‑140 (MQ=11)
                                                                            gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGTAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                              <  1:2585729/140‑1 (MQ=255)
                                                                            gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                              <  2:3922388/140‑1 (MQ=255)
                                                                            caacccGGACCCCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                              <  2:4804837/136‑1 (MQ=255)
                                                                               gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                           >  1:5904119/1‑140 (MQ=255)
                                                                               gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGCGACGCCGGACCAGCCGGAGCGGCAGGAGCTCGCGGGGAAGCGGGACCAAACGGACCGACGGGAGCCACCgg                                                           >  1:5800946/1‑140 (MQ=14)
                                                                               gCCGGACCACCAGGATCTACAGGAGCTTGGGGGTAAGCCGGACCTACCGGAGCTACAGGAGATACGGGGTAAGCGGGACCAGCCGTATCGAGAGGAGATACCGGGGAAGCCGGACCAACCGGAGCGACGTGAGCCACAgg                                                           <  2:596025/140‑1 (MQ=9)
                                                                                 cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACGGGCGCCGCCGGCACAGCCGGAGCGCCGGGAGcccacgccg                                                         >  2:5260600/1‑133 (MQ=11)
                                                                                    accccacGGAGCTCCCGGAGCCACCGGCGCCACCGGACCAACCGGAGCCCCAGGAGCTCCCGGCCCAGCCGGACCCCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTa                                                      <  2:896402/134‑1 (MQ=9)
                                                                                    aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                                             >  2:5820955/1‑69 (MQ=35)
                                                                                    aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                                             <  1:6382787/69‑1 (MQ=35)
                                                                                    aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                                             <  1:6657940/69‑1 (MQ=35)
                                                                                    aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                                             >  2:6382787/1‑69 (MQ=35)
                                                                                    aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAACGGGGGAACCCGGACCATCCGGAGCGGGGGGATCCGCAggcggga                                                      >  2:5430110/1‑135 (MQ=11)
                                                                                    aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAGCCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCGACCGGGGcga                                                      >  2:4423127/1‑138 (MQ=11)
                                                                                        gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTAc                                                                                                                                      >  1:1442238/1‑56 (MQ=35)
                                                                                         ccGGAGCCACAGGTTCTACCGGGGATGGCCGACGACCCGGAGCGACAGGAGCTACCGCGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                                 <  2:672031/140‑1 (MQ=14)
                                                                                         caggAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                              >  2:6127079/3‑127 (MQ=255)
                                                                                                  cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                              >  1:3699396/1‑118 (MQ=255)
                                                                                                          cccGGGGAAGCCGGACCTACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                <  2:1286250/139‑1 (MQ=255)
                                                                                                               ccaccccGGACCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGa                           <  1:1024975/135‑1 (MQ=17)
                                                                                                                gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGACGCCGGACCACCCGGAGCTACGGGAGCCCCAGGGGCTACTGGGTACGTCGCACCAGCCGGAGCGAc                          >  1:746543/1‑140 (MQ=18)
                                                                                                                gAAGCCGGACCAACCGGAGCGACAGGAGCCACCGGGGAAGCCGGACCAGGCGGAGCGCCAGGAGCGACCGGCGAAGCCGGACCACCCGGAGCGACGGGCGCGACAGGAGCACCGGGCGCCGGCGGACCAGCCGCAGCggc                          >  2:371827/1‑138 (MQ=14)
                                                                                                                   gCCGGACCAACCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGCCCGGGGACGCGGGACCTACCGGTGCGACGGGAGCCAAAGCTCCAACGGGGGAAGGCGGAACAGGCGCAGAGACatg                       >  2:2613489/1‑138 (MQ=2)
                                                                                                                   gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGACGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                       >  2:3635558/1‑140 (MQ=255)
                                                                                                                   gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                       >  1:1495121/1‑140 (MQ=255)
                                                                                                                   gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                       >  1:3343241/1‑140 (MQ=255)
                                                                                                                   gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                       <  2:1494821/140‑1 (MQ=255)
                                                                                                                   gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                       >  1:5237475/1‑140 (MQ=255)
                                                                                                                   gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTACTGGGGAAGACGGACCAGCCGGAGCGAccgc                       >  2:5520921/1‑137 (MQ=255)
                                                                                                                   gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCAGGACCAGCCGGAGCGACAGGATCTACCGGGGAACCCGGACAAACAGAAGAGACGGGAGCCACAGGGGCGACGGGGGACGTCGGACCAGCCGGAGCGACAgg                       >  1:3561517/1‑140 (MQ=18)
                                                                                                                   accGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGTGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                       >  2:5171479/2‑140 (MQ=14)
                                                                                                                        aCCAACCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGCAGCGAGCGGAGCTCCCGCCGACTCCGGACCAACCGGAGCGCGGGGAGCCACCGGCGCGACTGGGGACCTCGGACCGGCCGGAGCGCCCGGAGCtc                  >  2:1974320/1‑139 (MQ=12)
                                                                                                                        aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa                  >  2:7300613/1‑140 (MQ=255)
                                                                                                                           aaCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCg               >  2:7233493/1‑140 (MQ=255)
                                                                                                                             cgggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg             >  2:168127/3‑140 (MQ=18)
                                                                                                                             ccGGAGCGACAGGAGCTACCGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                         <  1:6803234/96‑1 (MQ=255)
                                                                                                                             ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg             >  1:606748/1‑140 (MQ=255)
                                                                                                                                 aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg         >  1:1980641/1‑140 (MQ=255)
                                                                                                                                 aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg         <  1:975370/140‑1 (MQ=255)
                                                                                                                                  gccaCAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGc        >  1:5416058/4‑140 (MQ=18)
                                                                                                                                  gccaCAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGc        >  2:535828/4‑140 (MQ=18)
                                                                                                                                   cgccAGGAGCGACCGGCGCCGCCCGACCCGCCGGAGCGACAGGAGCTTCCTGGGCAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGcc       <  1:7627336/137‑1 (MQ=14)
                                                                                                                                     aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg     <  1:4115359/140‑1 (MQ=255)
                                                                                                                                      cAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa    >  1:6947327/1‑140 (MQ=255)
                                                                                                                                      cAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGACAGGTGCTACCGGGGAAGCCGGACAAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa    <  2:1630837/140‑1 (MQ=17)
                                                                                                                                      cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg         >  2:1194869/1‑135 (MQ=255)
                                                                                                                                      cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg         <  1:1194869/135‑1 (MQ=255)
                                                                                                                                      cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACTGGGGACGTCGGACCAGCCGGAGCCACAGGAGCTACCGGGGCAGCCGGa    >  1:4289332/1‑140 (MQ=255)
                                                                                                                                       aGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc   >  2:6671454/1‑140 (MQ=17)
                                                                                                                                       aGGCGCTACTGCGGACGACGGACCAGCCGGAGCGACAGGAGCTTCAGGGGATTCCGCCCCACCCGGAGCGTCGGGAGCCACAGGTGCTACTGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc   <  2:5286077/140‑1 (MQ=14)
                                                                                                                                       aGGCGCTACGGGGGACGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc   <  2:6603823/140‑1 (MQ=255)
                                                                                                                                        ggAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAAAGGAGCTACCGGGGAAGCCGGAcc  >  2:17254/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                         
CGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACC  >  NZ_CP034943/607550‑607825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: