BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,702	C→T	20.5%	A182V (GCC→GTC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,702	0	C	T	20.5%	668.2 / 192.0	365	A182V (GCC→GTC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base C (165/121); new base T (62/11); total (228/137)
Fisher's exact test for biased strand distribution p-value = 1.00e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

Only 100 of 373 total aligned reads displayed.

GCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGG‑GAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGG  >  NZ_CP034943/607563‑607840

gCCCCGGGCGCCCCAGGTGCTCCTGGGGATCCCGGACCAGCCGGAGCGACAGGAGCTACCGGG‑GAAGCGGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                                                            <  2:1253271/140‑1 (MQ=11)

    cGGGAGCCACAGGGGCTAGTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGG‑GAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGGCGGAGCGACAGGAGCTACCGGGGAAGCCggc                                                                                                                                        >  1:801250/1‑139 (MQ=255)

     gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGG‑GAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGGCAGGAGCAACCGGGGCCGCCggcg                                                                                                                                       >  2:2409944/1‑138 (MQ=11)

         gCCACAGGGGCTCCTGGGGAAGCCGTACCAGCCGGAGCGACAGGAGCTACCGTG‑GAAGCCGGACCAGCCGGAGCGACAGGAGCCACCGGTGCACCAGGCCCAGCCGCAGCGACCGGAGCAACAGGCGAATCCCGAcatga                                                                                                                                   >  1:4555594/1‑136 (MQ=2)

          ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGG‑GAAGCCGGACCAGCCGGAGCGACAGGAGCCACCGGGGCCGCCGGACCACCCGGAGCGACGGGATCGCCAGGGGGAGCGGGAGCTGcc                                                                                                                                  >  1:2317326/1‑140 (MQ=11)

           cacaGGGGCTACTGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGG‑GAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCg                                                                                                                                 >  2:3890964/1‑140 (MQ=255)

             caGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGG‑GAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGa                                                                                                                               >  2:3476655/1‑140 (MQ=255)

                  gCTACGGGGGAAGCGGGACCAGCCGGAGCGACAGGAGCTACCGGT‑GAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                          <  1:3381442/140‑1 (MQ=255)

                   cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGGGACAGGG‑AAAGCAGTACCAGCCCGAGCGACCGGAGCAAACGGGGAATCCCGACCAACCGGAGCGACAGGAGCTACGGGGGGAGCCGGGCGAGCCGGCGGGAcc                                                                                                                         >  1:3975513/1‑139 (MQ=9)

                   cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGG‑GAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACa                                                                                                                         >  2:4855992/1‑140 (MQ=40)

                     aCTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGG‑GAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGGGAAGCCGCACACGGCGGAGCGACAGGAGCTACCGGGGCTGCCGGGGCGGCCGCGGCGACggg                                                                                                                       >  2:1958747/1‑140 (MQ=11)

                     aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGG‑GAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGGCGCCGGAGCAGCCGGAGCGACCgg                                                                                                                       >  2:6535289/1‑140 (MQ=11)

                     aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGG‑GAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                          <  1:1451618/137‑1 (MQ=40)

                          ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGG‑GAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                  >  2:7415905/1‑140 (MQ=255)

                            aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGG‑GAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACTGGAGCTACCGGGGAAGACGGACAATCCGGAGCGCCAGCAGCCAca                                                                                                                >  2:4614005/1‑139 (MQ=11)

                            aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGG‑GAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTAACGGGGAAGCCGGAGCAGCCGGAGCGACAGGAGCTccc                                                                                                                >  1:955871/1‑140 (MQ=255)

                            aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGG‑GAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                                                                                                >  2:927265/1‑140 (MQ=255)

                            aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGG‑GAAGCCGGAACAGCCGGAGCGACAGGAGCTACCGGCGACTCCGGAACAACCGGAGCGACAGTAGCTCCCGGGGCAGCCGGAGCAGACAGAGCGACAGGaacacac                                                                                                                >  2:1118453/1‑134 (MQ=14)

                              gCCGGACCAGCCGGAGCGACAGGAGCTACCGGG‑GAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCAGCCGGACCAACCGGACCGACCGGAGCGACAGGGGATACCGGCCCAGCCGGAGCAACCGGAGCGACCgg                                                                                                              >  2:21023/1‑140 (MQ=14)

                               ccGGACCAGCCGGAGCGACAGGAGCTACCGGG‑GAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                             <  1:4855992/140‑1 (MQ=255)

                                   aCCAGCCGGAGCGACAGGAGCTACCGGG‑GAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                         <  1:441687/140‑1 (MQ=255)

                                       gCCGGAGCGACAGGAGCTACCGGG‑GAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGAAGCCgg                                                                                                     >  1:5470493/1‑140 (MQ=255)

                                            aGCGACAGGAGCTACCGGG‑GAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCaa                                                                                                >  1:6603823/1‑140 (MQ=255)

                                                 cAGGAGCTACCGGG‑GAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGa                                                                                           >  2:3220454/1‑140 (MQ=255)

                                                      gCTACCGGG‑GAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGACGCCGGACCAACCGGAGCGAc                                                                                      >  2:6724768/1‑140 (MQ=255)

                                                       cTACCGGG‑GAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACg                                                                                     <  1:1742167/140‑1 (MQ=40)

                                                        tACCGGG‑GAAGCCGGAGCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGCCCAACGGGAGCGACTGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACgg                                                                                    <  1:6723308/140‑1 (MQ=14)

                                                         aCCGGGCGACGCCGGACCAGCCGGAGCGACAGGAGCGACCTGCGAAGCCGGAGCAACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACgg                                                                                    <  1:4969422/140‑1 (MQ=14)

                                                         aCCGGG‑GAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                   >  2:4212782/1‑140 (MQ=255)

                                                          ccGGG‑GAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCAACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGACGCCGGACCAACAGGAGCGACGCGa                                                                                  >  2:310107/1‑140 (MQ=14)

                                                          ccGGG‑GAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGCCGCCGGACCAGCCGGAGCGACCGGAGCGACCGGGGAAGCCGGAGCAACCGGAGCGACGGGa                                                                                  >  2:1986010/1‑140 (MQ=14)

                                                                gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGTAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                             <  1:2585729/140‑1 (MQ=255)

                                                                gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                                                                                                                              <  1:4092974/91‑1 (MQ=37)

                                                                gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                             <  1:7415905/140‑1 (MQ=255)

                                                                caacccGGACCCCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                             <  2:4804837/136‑1 (MQ=255)

                                                                 aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                                            <  1:5901535/140‑1 (MQ=255)

                                                                   gCCGGAGCCGCGGGAGCGACAGGCGCTCCCGGGGAAGCCCGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                          <  2:6911451/140‑1 (MQ=14)

                                                                     cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACGGGCGCCGCCGGCACAGCCGGAGCGCCGGGAGcccacgccg                                                                        >  2:5260600/1‑133 (MQ=11)

                                                                      ggACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGc                                                                       <  1:2759902/140‑1 (MQ=255)

                                                                        aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                                                            >  2:5820955/1‑69 (MQ=35)

                                                                        aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                                                            >  2:6382787/1‑69 (MQ=35)

                                                                        aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGCACCCGGACCAACCGGAGCGACGGGAGCCACAGGGGcga                                                                     >  2:3956117/1‑138 (MQ=255)

                                                                        aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTa                                                                     >  2:5196651/1‑140 (MQ=255)

                                                                        aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAACGGGGGAACCCGGACCATCCGGAGCGGGGGGATCCGCAggcggga                                                                     >  2:5430110/1‑135 (MQ=11)

                                                                        aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAGCCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCGACCGGGGcga                                                                     >  2:4423127/1‑138 (MQ=11)

                                                                                      cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                             <  2:3699396/118‑1 (MQ=255)

                                                                                      cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                             >  1:3699396/1‑118 (MQ=255)

                                                                                            cTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGcc                                                 <  1:684514/140‑1 (MQ=255)

                                                                                              aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGCCCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGCCCAACCGCAGCGACGGGAGCGACAGGGGCTACAGGGGACGGCGGACCAGCCgg                                               >  2:5131697/1‑140 (MQ=17)

                                                                                                   ccaccccGGACCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGa                                          <  1:1024975/135‑1 (MQ=17)

                                                                                                    gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAATCCGGTCCAACCGGAGCGCCCGGAGCCACAGGGGCTACAGGGGACGACGTACCAGCCGGAGCGAc                                         >  2:3778639/1‑140 (MQ=17)

                                                                                                     aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCCACAGGGGCAACCGGCGAAGGCGCACCAGCCGGAGCGACa                                        >  2:6060580/1‑140 (MQ=14)

                                                                                                      aaccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAg                                       >  2:1701759/3‑140 (MQ=14)

                                                                                                       gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                      >  1:7022677/1‑140 (MQ=255)

                                                                                                       gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                      <  2:1494821/140‑1 (MQ=255)

                                                                                                       gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                      >  1:1495121/1‑140 (MQ=255)

                                                                                                       gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                      >  1:5237475/1‑140 (MQ=255)

                                                                                                       gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                      >  1:3343241/1‑140 (MQ=255)

                                                                                                       gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTACTGGGGAAGACGGACCAGCCGGAGCGAccgc                                      >  2:5520921/1‑137 (MQ=255)

                                                                                                       gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCAGGACCAGCCGGAGCGACAGGATCTACCGGGGAACCCGGACAAACAGAAGAGACGGGAGCCACAGGGGCGACGGGGGACGTCGGACCAGCCGGAGCGACAgg                                      >  1:3561517/1‑140 (MQ=18)

                                                                                                       accGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                      >  2:701367/2‑140 (MQ=14)

                                                                                                               aaCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCg                              >  2:7233493/1‑140 (MQ=255)

                                                                                                                 ccGGAGCGACAGGAGCTACCGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                        >  2:6803234/1‑96 (MQ=255)

                                                                                                                 ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg                            >  1:606748/1‑140 (MQ=255)

                                                                                                                  gggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGCACCAGCCGTAGCGACAGGAGCTACCcggg                           >  2:103840/2‑140 (MQ=17)

                                                                                                                     cgcgcCAGGCGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGACGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                        <  1:2101350/139‑1 (MQ=255)

                                                                                                                     aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                        >  1:1980641/1‑140 (MQ=255)

                                                                                                                      gccaCAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGc                       >  2:535828/4‑140 (MQ=18)

                                                                                                                          cAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGACAGGTGCTACCGGGGAAGCCGGACAAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                   <  2:1630837/140‑1 (MQ=17)

                                                                                                                          cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                        <  1:1194869/135‑1 (MQ=255)

                                                                                                                          cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                        >  2:1194869/1‑135 (MQ=255)

                                                                                                                          cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                   >  2:7571664/1‑140 (MQ=255)

                                                                                                                          cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACTGGGGACGTCGGACCAGCCGGAGCCACAGGAGCTACCGGGGCAGCCGGa                   >  1:4289332/1‑140 (MQ=255)

                                                                                                                           aGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                  >  2:6671454/1‑140 (MQ=17)

                                                                                                                           aGGCGCTACTGCGGACGACGGACCAGCCGGAGCGACAGGAGCTTCAGGGGATTCCGCCCCACCCGGAGCGTCGGGAGCCACAGGTGCTACTGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                  <  2:5286077/140‑1 (MQ=14)

                                                                                                                               gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACGGGCGCTACTGGGGACGTCGCACCAGCCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAAc              >  2:2688503/1‑140 (MQ=18)

                                                                                                                               gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc              >  2:4093816/1‑140 (MQ=255)

                                                                                                                               gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc              >  1:530986/1‑140 (MQ=255)

                                                                                                                               gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc              >  1:538419/1‑140 (MQ=255)

                                                                                                                               gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc              >  1:6354850/1‑140 (MQ=255)

                                                                                                                                  actggggacgTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCCGCCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAACCGG           >  2:1351380‑M1/4‑140 (MQ=255)

                                                                                                                                  actggggacgTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCCGGGAAGCCGGACCACCCGc           >  2:3278598‑M1/4‑139 (MQ=255)

                                                                                                                                  actggggacgTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCCGGGAAGCCGGACCAACCGc           >  2:868399‑M1/4‑139 (MQ=255)

                                                                                                                                  actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg                            <  1:526498/120‑1 (MQ=255)

                                                                                                                                   ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGt                                                           >  2:182496/1‑91 (MQ=255)

                                                                                                                                   ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGA          >  2:4299593‑M1/1‑140 (MQ=255)

                                                                                                                                   ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGA          >  2:6247569‑M1/1‑140 (MQ=255)

                                                                                                                                   ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGA          <  1:927265‑M1/140‑1 (MQ=255)

                                                                                                                                   ccGCGGAAGCCGGCCCAGCCGGACCGACAGGCGCTCCCGGGGATTCCGGCCAAACCGGAGCGTCGGGAGCCACAGGTGCTACTGGGGACGGCGGAACAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGA          <  1:2073871‑M1/140‑1 (MQ=255)

                                                                                                                                       ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGa      >  1:708934/1‑140 (MQ=255)

                                                                                                                                       ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGa      >  1:3906679/1‑140 (MQ=255)

                                                                                                                                       ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGa      >  1:3264410/1‑140 (MQ=255)

                                                                                                                                        gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                                            >  1:4702809/1‑69 (MQ=255)

                                                                                                                                        gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc     >  1:3109652/1‑140 (MQ=255)

                                                                                                                                        gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc     >  1:2724281/1‑140 (MQ=255)

                                                                                                                                        gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc     >  2:5005506/1‑140 (MQ=255)

                                                                                                                                        gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCAACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc     >  1:5735671/1‑140 (MQ=255)

                                                                                                                                         acgCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                          <  1:130613/68‑1 (MQ=255)

                                                                                                                                         aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                          <  2:2744007/70‑1 (MQ=255)

                                                                                                                                           gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGcc                                                                               <  2:6127658/63‑1 (MQ=37)

GCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGG‑GAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGG  >  NZ_CP034943/607563‑607840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`