Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 634,108 C→A 7.6% V555F (GTT→TTT)  EO946_RS03330 ← ABC‑F family ATP‑binding cassette domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943634,1080CA 7.6% 1098.6 / 24.5 395V555F (GTT→TTT) EO946_RS03330ABC‑F family ATP‑binding cassette domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (70/282);  new base A (41/2);  total (111/284)
Fisher's exact test for biased strand distribution p-value = 2.57e-23
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 399 total aligned reads displayed.

AGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCTATATGGTTTGAAGAAAGCTCCAATACTCTTGTG  >  NZ_CP034943/633969‑634247
                                                                                                                                           |                                                                                                                                           
     ttCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTGGCTTGTTTTTCTTCGTCTTATGCAAGTTTTGAGTGAGGGTTGACCGaat                                                                                                                                        >  1:7257192/1‑139 (MQ=255)
     ttCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTATTTTTATTATTCATATGAAAGTTTTTTTTGAGGCTTAAACGAAg                                                                                                                                        >  2:773605/1‑140 (MQ=255)
       cATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGGGGTGGTTGTTTTTCCAATCGTTTTATTAGTAAAATGAAAGTTTTGATGCAGGCTTTAACGAAGCa                                                                                                                                      >  1:5570634/1‑140 (MQ=255)
       cATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGGCGTTGGCGGTCTTTGGTTTTGCCGTGTTTTTGTTGTTGCTATTCACCTTTTGAGTCAGGGGTAACAGAAGCa                                                                                                                                      >  1:464707/1‑140 (MQ=255)
        aTCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTGTTTCTGTTTGCATTATTTTTGTTCTTCATATGAAAGTTTTGGGTCAGGCTTAACCCAAACAg                                                                                                                                     >  2:4651064/1‑140 (MQ=255)
           gTTATGACTAATGTTTGCGTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCGTTCATTTTCCATTCTTTTTCTTCTTCATATGCACGTTTTGACTCAGGCTTAACCGAAGCAGGCg                                                                                                                                  <  1:1090621/140‑1 (MQ=255)
               tGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGTTTCTTTTTGCAGTATTTTTGTTCGTCATATTAAAGTTTTGAGTCACGCGTTACCACATCAGTCGtttt                                                                                                                              >  2:6127445/1‑140 (MQ=255)
                    aaTGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCgg                                                                                                                         <  2:3744438/140‑1 (MQ=255)
                     aTGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTGGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGa                                                                                                                        <  2:4212558/140‑1 (MQ=255)
                      tGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTCTTTTTACAATCTTTTTCTTCTTAATATGAACGTTTTTAGTCAGGCTTAAACGAAGCCGGCGTTTTTTCGGAt                                                                                                                       >  2:3846627/1‑140 (MQ=255)
                      tGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTACATTCTTTTTCTTATTCAAATTAACGTTTTTAGTCAGGCTTAAACCAAGCAGGCGTTTTTTCGGAt                                                                                                                       >  2:1883320/1‑140 (MQ=255)
                      tGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTATTTTTGCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACACAAGCAGGCGTTTTTTCGGag                                                                                                                       >  2:6603695/1‑139 (MQ=255)
                      tGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGTTTGTTTTTGCATTATTTTTATTATTAAAATTAAAGTTTTTTGTAAGGCTTAAACGAAGCAGGCGTTTTTTGGGAt                                                                                                                       >  2:2699010/1‑140 (MQ=255)
                          ttcttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTATTTATTTTTGCCTTCTTTTTCTTCTTGATATTAAAGTTTTGAGTAAGGCTTAAACCAAGCACGCGTGTTTTCGGATTCct                                                                                                                   >  2:4719045/1‑140 (MQ=255)
                          ttcgtaaaTGGTTTGAACAGTGGCTTCAAGTTCTTCAATCCGACGGAGTCGTTGGCCTTCTTTCTTTTGCCATTCTTTTTCTTCTTCCTATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCct                                                                                                                   <  2:70402/134‑1 (MQ=255)
                             ttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGGGGGGTGTTTTTTTGCAATGTTTTTTTTTTTGAAATGAACGGTTTTTGTGAGGCTTAAAACAAGCAGGCGTTTTTTGGGATTGCTCtt                                                                                                                >  1:3358542/1‑140 (MQ=255)
                                       tGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTGCCTTCTTTTTCTTCTTCATATGAACGTTTTGAGTGAGGCTTAAACGAACCAGGCGTTTTTTGGGATTGCTCTTTGTGATTAAt                                                                                                      >  2:508264/1‑140 (MQ=255)
                                        gAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCAtt                                                                                                     <  1:2612948/140‑1 (MQ=255)
                                        gAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCAtt                                                                                                     <  2:4516496/140‑1 (MQ=255)
                                            aGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTtct                                                                                                 <  2:3295557/140‑1 (MQ=255)
                                              tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTGTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTtcctg                                                                                               <  1:780679/140‑4 (MQ=255)
                                                gCTTCAATTTCTTCAATCCAACGGAGTCGTTGGGGTTCTTTCTTTTTGCATACTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTAGGATTACTCTTGCTGATTCATTTTGTCCaa                                                                                             >  2:352215/1‑140 (MQ=255)
                                                 cTTCAATTTCTTCAATCCGACGGCGTCGTTGGAGTTCTTTATTTTTCCATTCTTTTTCTTCGTCATAAGAACGATTTGAGTCAGGCTTAAACGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAAt                                                                                            <  1:1264591/140‑1 (MQ=255)
                                                    cAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTCATTTTCGATTGTTTTTGTTGTTAATATGAACGTTTTGAGTAATGCTTAACCGAAAAAAGCGTATTTTGGGAGTCCACATGCTTATTGATTTTATACAAttaa                                                                                         >  2:6805119/1‑138 (MQ=255)
                                                           ttcAATCCGACGGAGTCGTTGGGGGTGTTTCTTTTTGCCTTCTTTTTCTTGTTCATATGCACGGTTTTAGTGAGGGGTAACCCAAGCAGGCGTTTTTTCGGGTTCCGCTTGGGGATTGATTTTATCCAATTAAAgatgct                                                                                  >  2:76498/1‑140 (MQ=255)
                                                           ttcAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgct                                                                                  <  2:1188945/140‑1 (MQ=255)
                                                           ttcAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgct                                                                                  <  1:1329192/140‑1 (MQ=255)
                                                           ttcAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgct                                                                                  <  2:2090415/140‑1 (MQ=255)
                                                           ttcAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgct                                                                                  <  1:1918144/140‑1 (MQ=255)
                                                           ttcAATACGAAGGTTTCGTTTGCGTTCTTTATTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCTAAGCAGGAGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgct                                                                                  <  1:7348363/140‑1 (MQ=255)
                                                             cAATCCGAAGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGctct                                                                                <  2:368328/140‑1 (MQ=255)
                                                                     cGGAGTCGTTGGCGTTCTTTCTGTTTCCATTCTTGTTCTTCTGCATCTGAACGTTTGGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGttttttt                                                                        <  2:6888325/140‑1 (MQ=255)
                                                                       gAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTtct                                                                      <  2:5724843/140‑1 (MQ=255)
                                                                       gAGTCGTTGGCGTTCTTTCTTTTTCCATTATTTTTATTCTTCATATGAAGGTTTTGAGTCAGGATTAACCTAAGCATGAGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTtct                                                                      <  1:4060031/140‑1 (MQ=255)
                                                                          tCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCg                                                                   <  2:2599393/140‑1 (MQ=255)
                                                                          tCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCg                                                                   <  1:2417280/140‑1 (MQ=255)
                                                                          tCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCg                                                                   <  1:2763904/140‑1 (MQ=255)
                                                                          tCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCg                                                                   <  2:5217424/140‑1 (MQ=255)
                                                                          tCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCg                                                                   <  1:972425/140‑1 (MQ=255)
                                                                          tCGTTGGCGTTCTGTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCg                                                                   <  1:2302183/140‑1 (MQ=255)
                                                                          tCGTTGGCGTTCATTCTTTGTCCATTCTTTATCTTCTTCATATGAACGGTTTGAGTCAGGCTTAACCGAAGCATGCGTTTTTTCGGATTCCTCGTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCg                                                                   <  2:6419043/140‑1 (MQ=255)
                                                                                     ctttctttTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCg                                                        <  2:1307770/140‑1 (MQ=255)
                                                                                     ctttctttTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCg                                                        <  2:897394/140‑1 (MQ=255)
                                                                                       ttgtttgtCCATTCTTTTTCTTATTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTa                                                      <  1:6153398/137‑1 (MQ=255)
                                                                                       ttctttTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTa                                                      <  2:2257400/140‑1 (MQ=255)
                                                                                        tctttTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTTCTCTGTTTTTTTATCCGTATAATAATCGTaa                                                     >  1:1672970/1‑140 (MQ=255)
                                                                                         ctttTTCCTTTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCACGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAAt                                                    <  2:3601116/140‑1 (MQ=255)
                                                                                         ctttTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGTCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAAt                                                    <  1:1191118/140‑1 (MQ=255)
                                                                                         ctttTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAAt                                                    <  1:5046120/140‑1 (MQ=255)
                                                                                         ctttTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAAt                                                    <  1:4739350/140‑1 (MQ=255)
                                                                                         ctttTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAAt                                                    <  2:4832187/140‑1 (MQ=255)
                                                                                         ctttTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAAt                                                    <  1:1839409/140‑1 (MQ=255)
                                                                                         ctttTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAAt                                                    <  2:2329533/140‑1 (MQ=255)
                                                                                         ctttTTACCTTCTTTTTATTATTAATATGAACGTTTTGAGTCATGATTATCCGAATCTGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAAt                                                    <  2:341753/140‑1 (MQ=255)
                                                                                         ctttGTCCATTCTTTTTCTTCGTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAAt                                                    <  2:1859992/140‑1 (MQ=255)
                                                                                         ctttGTCCATTCTTGTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAAt                                                    <  2:7108612/140‑1 (MQ=255)
                                                                                              tCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGTCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTccc                                               <  1:3822751/140‑1 (MQ=255)
                                                                                              tCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTccc                                               <  2:3063895/140‑1 (MQ=255)
                                                                                              tCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTccc                                               <  1:3045453/140‑1 (MQ=255)
                                                                                              tCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTccc                                               <  2:1668017/140‑1 (MQ=255)
                                                                                               ccATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATAGTAATCTACCa                                              >  2:5519260/1‑140 (MQ=255)
                                                                                                 aTTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGa                                            <  1:4366642/140‑1 (MQ=255)
                                                                                                 aTTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGa                                            >  1:7051796/1‑140 (MQ=255)
                                                                                                   tgtttttCTCCTTCAGATGAACGTTTTGCGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCGGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa                                          <  2:7385104/138‑1 (MQ=255)
                                                                                                   tCTTTTTCTTCTTCATCTGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa                                          <  1:60038/140‑1 (MQ=255)
                                                                                                   tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa                                          <  1:4189526/140‑1 (MQ=255)
                                                                                                   tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa                                          <  2:4434208/140‑1 (MQ=255)
                                                                                                   tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa                                          <  1:3725588/140‑1 (MQ=255)
                                                                                                   tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa                                          <  1:4485249/140‑1 (MQ=255)
                                                                                                   tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa                                          <  1:4806816/140‑1 (MQ=255)
                                                                                                   tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa                                          <  2:1418878/140‑1 (MQ=255)
                                                                                                   tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATAGTAATCTCCCAGATa                                          >  2:4291435/1‑140 (MQ=255)
                                                                                                   tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGATTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa                                          <  1:4000634/140‑1 (MQ=255)
                                                                                                   tCTTTTTCGTCTGCCTATGAACGTATTGAGTCAGGCTTAACCGAAGCACGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCACTTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa                                          <  2:4625322/140‑1 (MQ=255)
                                                                                                   tCTTGTTCTACTTCATATGTAGGTTTTGATTCCCGCTTAACAGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAACCTGCTCTGTTTGTTTCTCCGTATAATAATCGTAATCTCCCAGATa                                          <  2:4313690/140‑1 (MQ=255)
                                                                                                     tttttCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATAct                                        <  2:2440156/140‑1 (MQ=255)
                                                                                                     tttttCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATAct                                        <  2:1804062/140‑1 (MQ=255)
                                                                                                     tttgtCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATAct                                        <  2:6199269/140‑1 (MQ=255)
                                                                                                      ttttCTTCTTCATATTAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATActc                                       <  2:7208114/140‑1 (MQ=255)
                                                                                                       tttCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATActct                                      >  2:603966/1‑140 (MQ=255)
                                                                                                        ttcttcttcATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACtctt                                     <  2:2504065/140‑1 (MQ=255)
                                                                                                            tcttcATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCtat                                 <  1:1569196/140‑1 (MQ=255)
                                                                                                            tcttcATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCtat                                 <  2:5980928/140‑1 (MQ=255)
                                                                                                               tcATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTtct                                                                      <  1:2098997/100‑1 (MQ=255)
                                                                                                               tcATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTACCAGATACTCTTCTATATg                              >  1:6027149/1‑140 (MQ=255)
                                                                                                                 atatGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCTATATGGt                            <  1:5151059/140‑1 (MQ=255)
                                                                                                                         gTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTCATCTCCCAGATACTCTTCTATATGGTTTgaagaa                    <  1:6270098/140‑1 (MQ=255)
                                                                                                                         gTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCTATATGGTTTgaagaa                    <  2:739662/140‑1 (MQ=255)
                                                                                                                         gTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCTATATGGTTTgaagaa                    <  1:7103380/140‑1 (MQ=255)
                                                                                                                         gTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCTATATGGTTTgaagaa                    <  2:6731458/140‑1 (MQ=255)
                                                                                                                         gTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCTATATGGTTTgaagaa                    <  1:6551313/140‑1 (MQ=255)
                                                                                                                         gTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCAGATTCATTTTCTCCAATAAAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCTATATGGTTTgaagaa                    <  2:1603018/140‑1 (MQ=255)
                                                                                                                         cttttGAGTCAGGCTTACACGACGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCTATATGGTTTgaagaa                    <  2:3968016/139‑1 (MQ=255)
                                                                                                                           tttGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCTATATGGTTTGAAGAAAg                  >  2:2900456/1‑140 (MQ=255)
                                                                                                                           tttGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATGCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCTATATGGTTTGAAGAAAg                  <  1:6851554/140‑1 (MQ=255)
                                                                                                                            ttGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCTATATGGTTTGAAGAAAGc                 <  1:2886389/140‑1 (MQ=255)
                                                                                                                            ttGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCTATATGGTTTGAAGAAAGc                 <  1:6698115/140‑1 (MQ=255)
                                                                                                                                   aGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACGCTTCTCTATGGTTTGAAGAAAGCTCCAATa          >  1:7688309/1‑140 (MQ=255)
                                                                                                                                           ccGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCTATATGGTTTGAAGAAAGCTCCAATACTCTtgtg  >  2:7075008/1‑140 (MQ=255)
                                                                                                                                           ccGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGAGTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCTATATGGTTTGAAGAAAGCTCCAATACTCTtgtg  >  2:5399122/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCTATATGGTTTGAAGAAAGCTCCAATACTCTTGTG  >  NZ_CP034943/633969‑634247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: