Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 755,531 A→C 14.2% Q22H (CAA→CAC EO946_RS03880 → rhamnogalacturonan acetylesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943755,5310AC14.2% 708.7 / 28.0 346Q22H (CAA→CACEO946_RS03880rhamnogalacturonan acetylesterase
Reads supporting (aligned to +/- strand):  ref base A (203/73);  new base C (2/63);  total (206/140)
Fisher's exact test for biased strand distribution p-value = 1.15e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 350 total aligned reads displayed.

CACCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCCGGATG  >  NZ_CP034943/755392‑755670
                                                                                                                                           |                                                                                                                                           
cACCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGCATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCaa                                                                                                                                             >  2:4745048/1‑140 (MQ=255)
  ccGGTTTTTTCAAGGGAATAGCTGTCCATTATTGATGCCATATGAAAAGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAgg                                                                                                                                           <  2:6555312/140‑1 (MQ=255)
  ccGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAATAAAACGAGGGTGAATGAGATATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAgg                                                                                                                                           <  2:2236378/140‑1 (MQ=255)
            caagttaaGAGCTGTCAATGATTTATTCCATATGAAACTCAAATAACGAGGTGGAATGAGACATGTGAAATCACATTTATCTTTCTGGAGATTCAAATTTTCAAACTTATTGAGACAGCAAAAATCAAGGGGGGTggggg                                                                                                                                 <  2:3064534/134‑1 (MQ=255)
                          tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                                   >  1:1117577/1‑140 (MQ=255)
                          tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                                   >  1:1338422/1‑140 (MQ=255)
                          tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                                   >  1:2557171/1‑140 (MQ=255)
                          tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                                   >  2:1481372/1‑140 (MQ=255)
                            cATGATTGATGCACTATGAAACGAACAAAACTAGCGGGAATTAGAAATGGCAAATCACATTTATATTGCTGTCGTTTCAACTGTTAAAACGTATGGAGACACCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGc                                                                                                                 <  2:3406011/140‑1 (MQ=255)
                            cATGATTGATGCAATATGAAACGAAAAAAAAGATGGGGAATGAGAAATCGCAAATCACATTTATATTTCTGGAGATTCAACTGTTAAAACGTATGGAGACAGCACAAATCAAGGCGGGTGGGGGCAGATTCTCGGCTCGc                                                                                                                 >  2:7470256/1‑140 (MQ=255)
                             aTGATCGATGCCATATGAAACGAACACAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCTACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCa                                                                                                                <  2:1434191/140‑1 (MQ=255)
                               gattgatGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATc                                                                                                              >  2:2494463/1‑140 (MQ=255)
                               gattgatGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATc                                                                                                              >  1:3675616/1‑140 (MQ=255)
                               gattgatGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATc                                                                                                              >  2:5382001/1‑140 (MQ=255)
                                  tgaacccATAACAAACGAAAAAAACGAGGCCGAATGACAAATCGCAAATCACATTTATCTTGCTGGCGATTCACCTGTTCAAACGTATGGAGACAGCACAAATCCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGc                                                                                                           <  2:322931/135‑1 (MQ=255)
                                   gatGCCATATGAAACGAAAAAAACGAGGGGGAATGACAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTAGGGAGACACCACAACCCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGcc                                                                                                          <  1:6826198/140‑1 (MQ=255)
                                   caccccATACGACACCACAAAAACGAGGCGGAATGAGAAATGGCAAAACACATTGATCGTGCTGGCGATTCACCTCCCCACACCCCGGGAGCCAGCACCACTCCACCGGCGCGGGGGCAGTTTCTCGGCTCGCATCTGcc                                                                                                          <  2:3675616/136‑1 (MQ=255)
                                    atGCCATATGAAACGAAACAAACGAGGGGGACTGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAATCGTATGGAGACATCACAAATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                         >  2:2386464/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGTGGGGGACGGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGAACGCACAAAGCAAGGGGGGTGGGGGCAGTGTCTCGGCTCGCATCTGCCg                                                                                                         >  1:5712835/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCACACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                         >  2:7094194/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACACATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                         >  1:4402415/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                         >  1:287245/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                         >  1:1000967/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                         >  1:938389/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                         >  1:4678812/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                         >  1:4028987/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                         >  1:3054788/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                         >  1:3183466/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                         >  1:3273832/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                         >  1:356957/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                         >  1:360312/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                         >  2:5074039/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTAAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGcgg                                                                                                         >  1:2664278/1‑138 (MQ=255)
                                    atGCCATATGAAAATAAAAAAACGAGTGGGAATGATAAATTTCAAATCACATTTATCTTTCTGGCGATTCACCTGTTCAAACGTATGGAGACAGCACAACTCCAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                         <  2:4570420/140‑1 (MQ=255)
                                          tatGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGTCGATTAAACTGTTCAAACGTATGGAGACAGCACAAATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCat                                                                                                   <  2:3197119/140‑1 (MQ=255)
                                          tatGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAATGGGGTTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCat                                                                                                   >  2:4645972/1‑140 (MQ=255)
                                          tatGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCat                                                                                                   >  1:7527852/1‑140 (MQ=255)
                                          tatGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGATCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCat                                                                                                   >  2:2578635/1‑140 (MQ=255)
                                           atGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCata                                                                                                  >  1:7113057/1‑140 (MQ=255)
                                           atGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCata                                                                                                  >  1:5012787/1‑140 (MQ=255)
                                              tatctaaaaaaaTGAGTTGTAATTAGAATTGTCATTTCATTTTTTTCTTGCTTGCGATTCAACTTTTCAAACGTATTGAGACAGCACACATCAAGGGGGGTGGGCGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                               <  2:3863939/135‑1 (MQ=255)
                                              aacataaaataaCGATGGTTAATGATCAATGGCAATTCAACTTTATCTTGCTGTCTATTCAACTGTTCAAACCTATGCAGACCGCACACATCAAGGGGGCGGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                               <  2:3533412/135‑1 (MQ=255)
                                              aaaCGAAAAAAACTAGGGGTAATGAGAACTGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACACAACAAGCGGGGTGGCGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                               <  2:1583316/140‑1 (MQ=255)
                                              aaaCGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGAGTCAACTGTTCAAACGTATCGACACAGCACAACTCCCGGGGGGGGGGGGCAGTTGCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                               <  1:170905/140‑1 (MQ=255)
                                               aaCGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCa                                                                                              >  2:3366542/1‑140 (MQ=255)
                                               aaCGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCa                                                                                              >  2:6210691/1‑140 (MQ=255)
                                                actacaaaaaCGAGGCTGAATGACACATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAACTCACGGGGCGGGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                             <  1:2490432/135‑1 (MQ=255)
                                                accaaaaaaaCGAGGGCGCATGAGAAATGGCAAATCACATTTATCTTGCGGGCGATTCAACTGTCCCAACGTATGGCGCCAGCACAAATCCCGGGGGGTGGGGGCAGTTTCGCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                             <  1:2241397/137‑1 (MQ=255)
                                                aCGAAAAAATCGAGTGTGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                             <  2:646014/140‑1 (MQ=255)
                                                  taaataaaCGAGTGTTAATGATAAATTCAAAATCACATTTATCTTGCTGGCGATTCTTCTGTTCAAACTTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGt                                                                                           <  2:801530/139‑1 (MQ=255)
                                                   aaaacaaCGTGCGCGAATCACACATGCCAAATCTCATTGATATTGCTGGCGAGGCACCTGCTCAACCGTTGGGAGACCGCAGCACTCACGGCGGCGCGGCGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTg                                                                                          <  1:4772723/140‑1 (MQ=255)
                                                        aaCGAGCGGCAATGAGACATGCCAAATCACATTTATCTTGCTGGCGATACAACTGTTCAAACGTATGGACACAGCACACATCCCGGGGGGGGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTaa                                                                                     <  1:800362/140‑1 (MQ=255)
                                                        aaCGACCGGCAAAGAGACATGGAAAATCACATTGATCGTGCTGGCGATTCTACTTAGCTCAGGTCTTTACACAGCACAAATCACGGCGGGTGGGGGCAGTTTCGCGGCTCGCATCTGCCGGAGAATATTCAAGTGATTaa                                                                                     <  2:1133999/140‑1 (MQ=255)
                                                          cGAGCGCGAATCAGAAATCCCAAATCACCTTTATCGAGCTCGCGATTCAACTGTACAACCGTATGGAGACACCACACACCCAGGGGGGCGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACa                                                                                   <  1:4195463/140‑1 (MQ=255)
                                                           gAGGGGCACTGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACag                                                                                  <  1:2733242/140‑1 (MQ=255)
                                                             tttgttaTGAGATATGTCTAATCACTTTTATCTTGCTGTCTATTCCACTGTTCCATCGTATGGATACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACagag                                                                                <  2:418438/134‑1 (MQ=255)
                                                                  aaTGATAAATGCCACCTCACCCTGATCTTGGTGTCTATTGAACTTTTGATCAGTATGGAGACAGCACAAATGAAGGGGGGTGTGTGCATTTTCTCGGATCGCATCTGCCGGAGCATATTCAAGTCATTAACAGAGCAATc                                                                           <  2:6155436/140‑1 (MQ=255)
                                                                         aaTGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGgaa                                                                    >  1:3140033/1‑140 (MQ=255)
                                                                             tcAAATGATATTTTTCTTGCTGGGGATTGATCTGTTCCTACGTTTGGAGTCTGCTCATATGCTGTGGGGTGGGGGCATTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGaagaag                                                                <  2:6056357/139‑1 (MQ=255)
                                                                                      aTTTATCTTGCTGGCGATTCAACTGTTCAACCGTATCGAGACAGCACAACACCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAAc                                                       <  1:6493239/140‑1 (MQ=255)
                                                                                        ttttCTTGCTGTCTATTTAAGTGTTCCTTCGTCTGGAGACTGCACTCAGCCCGGGGGGTGGTGTCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAAGAGAGCAATCGGGGGAAGAAGCTCGAAAACAt                                                     <  2:4385696/137‑1 (MQ=255)
                                                                                        ttAGCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCGTCTGCCGGAGCTTCTTCAGGTGATGACCCGAGCAATCGGGGGAAGTGGCTCGAAAACag                                                     >  1:640184/1‑139 (MQ=255)
                                                                                            cTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTtgt                                                 >  1:384739/1‑140 (MQ=255)
                                                                                                ctgcctcttCACCTGTTCACACTGATGGCCACCGCACACATCACGCGCGGTGCGTGCAGTTTCTCGTCTCGCATCTGCCGGAGCATATTCAAGTTATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                             <  2:2709592/133‑1 (MQ=255)
                                                                                                cTGGCGATTCAACTGTTCACCCGGCTGCAGACAGCAAAAATCTAGGGGGGGTGGGGCAGTTTCTAGGCTGTCATATGCATTAGCATATTCTAGTGTTTAACATAGCAATCGGGGGAAGAAGCTCTAAAACATGTGTTGaa                                             <  2:4348739/140‑1 (MQ=255)
                                                                                                cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                             >  1:7346702/1‑140 (MQ=255)
                                                                                                cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                             >  1:6031184/1‑140 (MQ=255)
                                                                                                 tgccGATTCAACTGTTATACCGTATTGAGCCAGCACCAACCCAGGGGGGGGGGGGCAGTGTCTCGGCTCGCATCTGCCGGAGCATATTCCAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAag                                            <  2:415843/137‑1 (MQ=255)
                                                                                                          taCTGTTCAAACGTATGGAGACAGCACAAATAAAGGGGGGTGGGGGCAGTTTCTCGGATCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAAACGGGGGAAGAAGCTCGAAAACAGTTGTGGAAGAGGGCAGGc                                   >  2:681850/2‑140 (MQ=255)
                                                                                                               ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAACCAGTTGTGGTAGAGGGCAGGCTTCAg                              >  2:4872240/1‑140 (MQ=255)
                                                                                                               ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                              >  1:3764080/1‑140 (MQ=255)
                                                                                                               ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                              >  1:3405497/1‑140 (MQ=255)
                                                                                                               ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                              >  1:7446813/1‑140 (MQ=255)
                                                                                                               ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                              >  1:2349144/1‑140 (MQ=255)
                                                                                                               ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTCCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                              >  2:7349588/1‑140 (MQ=255)
                                                                                                                 cAAACGTATGGAGACAGCACAACTCCAGGGGGGTGGGGGCAGGTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGc                            <  2:2854453/140‑1 (MQ=255)
                                                                                                                 cAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACAGTTGTGGAAGAGGGCAGGCTTCAGGc                            >  1:396142/1‑140 (MQ=255)
                                                                                                                   atggtctGGAGAGAGCACAAACCCCGGGGGGTGCTGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGa                          <  2:2301855/134‑1 (MQ=255)
                                                                                                                       tATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGACGAGGGCAGGCTTCAGGCGATTCt                      >  2:1716618/1‑140 (MQ=255)
                                                                                                                       tATCGAGCCCGCACAAACCCCGGGGGCGCGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGGTTCAGGCGATTCt                      <  2:6976427/140‑1 (MQ=255)
                                                                                                                         tGGATACAGCACAAATCAAGGGGCGTTGGGGCAGTTTCTCGTCTCGCATCTGCGGGAGCAGATAAAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACTATTGTGGAAGAGGGCAGGGTTACGGCGATTCTTg                    >  1:5476942/1‑140 (MQ=255)
                                                                                                                          ggcgaCAGCACCACCCGAGCGGGGTCGCGGCAGTGTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTACCAGAGCACTCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGa                   <  1:70048/137‑1 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGc                                                                                                           >  2:245320/1‑46 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt             >  1:5271202/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt             >  2:7648158/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt             >  1:7214210/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt             >  2:3506245/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt             >  2:598444/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGCGCAGGCTTCAGGCGATTCTTGATGTGAt             >  2:6627391/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGAATAACAGAGCAATCGGGGGAACAAGCTCGAAAACATTTCTGGACGAGGGCAGGATTCAGGCGATTCTTGATGTGAt             >  2:5161629/1‑140 (MQ=255)
                                                                                                                                 gCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAtt            >  1:1908599/1‑140 (MQ=255)
                                                                                                                                 gCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAtt            >  1:5854465/1‑140 (MQ=255)
                                                                                                                                  cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg           >  1:7198528/1‑140 (MQ=255)
                                                                                                                                  cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg           >  1:6686085/1‑140 (MQ=255)
                                                                                                                                  cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg           >  2:5239323/1‑140 (MQ=255)
                                                                                                                                  cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg           >  1:1492675/1‑140 (MQ=255)
                                                                                                                                  cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACAGTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg           >  2:3014158/1‑140 (MQ=255)
                                                                                                                                  cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATAATCAAGTGATTAACAGAGCAAAAGGGGGACGAAGCTCGAAATCATTTGTGGAAGAGGGGAGGCTTCTGGCGCTTCTTGAGGTGATTg           >  2:5733543/1‑140 (MQ=255)
                                                                                                                                       aTCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCCg      >  2:501642/1‑140 (MQ=255)
                                                                                                                                           aGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCCGGATg  >  1:4887125/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CACCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCCGGATG  >  NZ_CP034943/755392‑755670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: