Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 986,801 G→T 9.3% D372Y (GAT→TAT)  spoVR → stage V sporulation protein SpoVR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943986,8010GT 9.3% 872.5 / 12.5 374D372Y (GAT→TAT) spoVRstage V sporulation protein SpoVR
Reads supporting (aligned to +/- strand):  ref base G (146/169);  new base T (2/51);  total (148/226)
Fisher's exact test for biased strand distribution p-value = 1.47e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 378 total aligned reads displayed.

GCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATATTAAGCATTGGTA  >  NZ_CP034943/986664‑986940
                                                                                                                                         |                                                                                                                                           
gCGTGAAGCCTGGCTCAGGCCGGGAGAAAATCTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTGATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGAt                                                                                                                                           >  2:1166155/1‑140 (MQ=255)
gCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGAt                                                                                                                                           >  2:3256104/1‑140 (MQ=255)
          tGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAAtcg                                                                                                                                 >  1:7127514/1‑140 (MQ=255)
          tGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAAtcg                                                                                                                                 >  2:6845164/1‑140 (MQ=255)
            gCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCATAGATTATAAAAtcgtc                                                                                                                               >  1:2761663/1‑140 (MQ=255)
                aggctgtGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGTTATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGAGAGGATGTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACa                                                                                                                           <  1:4228437/140‑1 (MQ=255)
                aggcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACa                                                                                                                           <  1:4935113/140‑1 (MQ=255)
                aggcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACa                                                                                                                           <  1:600199/140‑1 (MQ=255)
                 ggcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACaa                                                                                                                          >  1:2386165/1‑140 (MQ=255)
                  gcaggGAGAACATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACaaa                                                                                                                         >  1:2910031/1‑140 (MQ=255)
                  gcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACaaa                                                                                                                         >  1:1722448/1‑140 (MQ=255)
                  gcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACaaa                                                                                                                         >  1:6074727/1‑140 (MQ=255)
                  gcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACaaa                                                                                                                         >  1:2812963/1‑140 (MQ=255)
                     gggAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCATTATTTGTTTCAAACACAGGGCAGAGATTATAAAATCGTCGACAAAGaa                                                                                                                      <  1:2613737/140‑1 (MQ=255)
                      ggAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAAt                                                                                                                     >  1:1005307/1‑140 (MQ=255)
                      ggAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAAt                                                                                                                     >  2:5787868/1‑140 (MQ=255)
                      ggAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAAt                                                                                                                     >  1:861500/1‑140 (MQ=255)
                      ggAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAAt                                                                                                                     >  1:6912452/1‑140 (MQ=255)
                            aaTGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAATCAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAAt                                                                                                               >  1:3725426/1‑140 (MQ=255)
                             aTGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATc                                                                                                              <  2:6912463/140‑1 (MQ=255)
                             aTGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATc                                                                                                              <  1:1488184/140‑1 (MQ=255)
                             aTGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATc                                                                                                              <  1:4710431/140‑1 (MQ=255)
                                 ttGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTa                                                                                                          >  1:7314659/1‑140 (MQ=255)
                                 ttGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTa                                                                                                          >  1:6481069/1‑140 (MQ=255)
                                  tGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAATCAGGGCAGATATTATAAAATCGTCGACAAAGAATGGAAATCGGTAc                                                                                                         >  2:1388338/1‑140 (MQ=255)
                                  tGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCTACAAAGAATGGAAATCGGTAc                                                                                                         >  2:6631482/1‑140 (MQ=255)
                                     ggTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTAcgcg                                                                                                      <  1:6995034/140‑1 (MQ=255)
                                     ggTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTAcgcg                                                                                                      <  2:4903027/140‑1 (MQ=255)
                                       tCATGGAAATTGAATCCGATATCACTCTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                                    <  1:1976698/140‑1 (MQ=255)
                                       tCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                                    <  2:5469377/140‑1 (MQ=255)
                                       tCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                                    <  2:5690391/140‑1 (MQ=255)
                                       tCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                                    <  1:5537045/140‑1 (MQ=255)
                                       tCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                                    <  1:1769311/140‑1 (MQ=255)
                                       tCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                                    <  1:7387028/140‑1 (MQ=255)
                                        cAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTCATTTGTTTCAAAAACAGGGCAGAGATGATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAcc                                                                                                   <  1:1911559/140‑1 (MQ=255)
                                         aGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCa                                                                                                  >  1:5196133/1‑140 (MQ=255)
                                          gggAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGa                                                                                                                       <  2:2083771/118‑1 (MQ=255)
                                          gggAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGa                                                                                                                       >  1:2083771/1‑118 (MQ=255)
                                          gggAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAg                                                                                                 >  2:7568057/1‑140 (MQ=255)
                                               aTTGAATCCGATATCTCTTTCATCCGCAATTATTTAACCAAAGAATTAGTCATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCCGAGATTATAAAATCATCGATAAACAGTGGAAGTCGGTGCGCGACCAGCTCGt                                                                                            <  1:2172693/140‑1 (MQ=255)
                                                ttGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTg                                                                                           >  1:6447126/1‑140 (MQ=255)
                                                ttGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTg                                                                                           >  2:3072318/1‑140 (MQ=255)
                                                      ccGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATg                                                                                     <  2:7162814/140‑1 (MQ=255)
                                                                tttCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATc                                                                                                              <  1:5892430/105‑1 (MQ=255)
                                                                  tCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAAcggc                                                                         >  1:1248536/1‑140 (MQ=255)
                                                                     tCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGc                                                                                                >  2:383784/1‑114 (MQ=255)
                                                                     tCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCggg                                                                      >  2:6369944/1‑140 (MQ=255)
                                                                      ccGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGt                                                                     >  1:4842552/1‑140 (MQ=255)
                                                                          aaTTACTTAACACAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTcc                                                                 <  2:4021018/140‑1 (MQ=255)
                                                                          aaTTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTcc                                                                 <  2:6501606/140‑1 (MQ=255)
                                                                                  aaCAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGa                                                         <  2:6138373/140‑1 (MQ=255)
                                                                                    cAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACa                                                       <  2:1248536/140‑1 (MQ=255)
                                                                                    cAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACa                                                       <  2:2911199/140‑1 (MQ=255)
                                                                                    aaaaaGACTTTGTTATGCGCGAGGATATTTATTTTTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACa                                                       <  1:7665831/139‑1 (MQ=255)
                                                                                              aGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAacgacg                                             >  2:263989/1‑140 (MQ=255)
                                                                                              aGTTAGGCGCGTGTATCTTTATTTGTTAAAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGATTGCAAATCGTTACGCGACCAGCTCGTGAGCTTGGGGTTAAACGGCGGGTTTCCTTATTTGACAGTAAacgacg                                             <  1:5334786/140‑1 (MQ=255)
                                                                                                ttATGCGCTCGTATCTTTATTTGTTTCAAAAACAGGGCAGATATTATAAAATGGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCAGGCGGGTAAACGGCGGGTTTCCATATTTGACATTAAACGACGGt                                           <  2:4822094/140‑1 (MQ=255)
                                                                                                ttATGCGCTAGTATCTTTATTTTTTTCAAAAACAGGGCAGATATTATAAAATCGTCGAAAAATAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGt                                           <  2:1018853/140‑1 (MQ=255)
                                                                                                     cgcgAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTa                                      >  1:4618513/1‑140 (MQ=255)
                                                                                                     cgcgAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTa                                      >  2:3140224/1‑140 (MQ=255)
                                                                                                         aGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTTACAGTAAACGACGGTGATTACATg                                  >  2:4389204/1‑140 (MQ=255)
                                                                                                          ggATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGa                                 >  2:423029/1‑140 (MQ=255)
                                                                                                                ttATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATa                           >  2:7707736/1‑140 (MQ=255)
                                                                                                                  tttttttTCAAAAACTGGGCAGAGATTATAAAATCGTCGACAAAGAAGGGAAATGGGAACGCGACCAGCTGGTGAGCATGGGGGTAAAGGGGGGGTTTCAATATTTGACAGTACACGACGGTGATTACATGAAAAATAAc                         <  1:7707736/139‑1 (MQ=255)
                                                                                                                  tttttttTAAAAAACAGGGCACATTTTATAAAATCGTCGAAAAATAATGGAAATCGGTACGCCACCCGCTCGTGAGCATGCGGTTAAACGGCGGGTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc                         <  1:6333460/139‑1 (MQ=255)
                                                                                                                  tttgttttAAAAAACAGGGAATATATTATAAAATCGTCTAAAAATATGGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCAATATTTGACAGTACACGACGGTGATTACATGAAAAATAAc                         <  1:3581151/135‑1 (MQ=255)
                                                                                                                  aTTTGTTTCAAAAACTGGGCATCTATTATAAAATCGTCGACAAAGATTGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc                         <  2:7450811/140‑1 (MQ=255)
                                                                                                                  aTTTGTTTCAAAAACAGGGCAGCCATTATAAAATCGTCGACAAAGAATGGAAATCGGTCCGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc                         <  2:2260544/140‑1 (MQ=255)
                                                                                                                  aTTTGTTTCAAAAACAGGGCAGATATTAAAAAATCGTCGACAAAGAAGGGAAATCGGTACGCGACCAGCTGGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTACACGACGGTGATTACATGAAAAATAAc                         <  1:5787868/140‑1 (MQ=255)
                                                                                                                  aTTTGTTTAAAAACCAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAAGCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc                         <  2:469187/140‑1 (MQ=255)
                                                                                                                  aTTTGTTTAAAAAACAGGGCATAGATTATAAATTCGTCGACAAATAATGCAAATGGGTACGCGACCAGCTCGTGCGCATGCGGTTAACCGGCGTTTTTCCATATTTGACATTAAACGACGGTGTTTACATGAAAAATAAc                         <  2:1552409/140‑1 (MQ=255)
                                                                                                                  aTTTGTTTAAAAAACAGGGCAGATTTTATAAAATCGTCGAAAAAGAATGGAAATCGTTACGCGACCCGCTCTTGAGCATGCGGGTAAACGGCGGTTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc                         <  2:6746635/140‑1 (MQ=255)
                                                                                                                  aTTTGTTTAAAAAACAGGGCAGACATTATAAAATCGTCGACAAACAATGGAAATCGGTACGCCACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc                         <  2:1797577/140‑1 (MQ=255)
                                                                                                                  aTTTGTTAAAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAATTGGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTTACATTAAACGACGGTGATTACATGAAAAATAAc                         <  2:6042850/140‑1 (MQ=255)
                                                                                                                  aTTGGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCTACAAAGAATGGAAAGCGGTACGCGACCAGCTCGTGAGCATGGGGGTAACCGGCGGGTTTCCATATTTGACATTAAACGACGGTGATTACATGAAAAATAAc                         <  2:1684098/140‑1 (MQ=255)
                                                                                                                     tGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAg                      <  2:450287/140‑1 (MQ=255)
                                                                                                                      gTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                                    >  1:5050635/1‑61 (MQ=255)
                                                                                                                      gTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGttt                                                                   >  1:3403805/1‑94 (MQ=255)
                                                                                                                      gTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGttt                                                                   <  2:3403805/94‑1 (MQ=255)
                                                                                                                      gTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGc                     >  1:1797577/1‑140 (MQ=255)
                                                                                                                      gTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGc                     >  2:506550/1‑140 (MQ=255)
                                                                                                                      gTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGc                     >  2:3491097/1‑140 (MQ=255)
                                                                                                                      gTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGc                     >  1:3652235/1‑140 (MQ=255)
                                                                                                                      gTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGAATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAAGAGc                     >  1:4485347/1‑140 (MQ=255)
                                                                                                                       tttCAAAAACAGGGCCGCGATTATAAAATCGTCGACAAAGAATGCAAATCGGTACGCGACCAGCTCGTGCGCATGCGGGTAACCGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAACAATAACGAGct                    <  2:500969/140‑1 (MQ=255)
                                                                                                                       tttCAAAAACAGGGCAGATATTATAAAATCGTCGACAAAGAATGTAAATCGGTACGCGACCAGCTCGTGAGCATGCGGTTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct                    <  2:245251/140‑1 (MQ=255)
                                                                                                                       tttCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGTTACGCGACCAGCTCGTTAGAATGCGGGTAAACGGCGGGTTTCCATATTTGAAAGTAAACGACGGTGATTACATGAAAAATAACGAGct                    <  2:279650/140‑1 (MQ=255)
                                                                                                                       tttAAAAAACTGGGCATATATTATAAAATCGTCGAAAAAGATTGGAAATCGGTACGCGACCAGCTGGTGAGCATGCGGGTAAACGGCGGGTTTCAATTTTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct                    <  1:6639212/140‑1 (MQ=255)
                                                                                                                       tttAAAAAACTGGGCATAGATTATAAAATCGTCGAAAAAGAATGTAAATCGTTACGCGCCCAGCTCGTGAGCATGCGGTTAACCGGCGGGTTTCCATATTTGACATTAAACGACGGTGATTACATGAAAAATAACGAGct                    <  1:916214/140‑1 (MQ=255)
                                                                                                                       tttAAAAAACAGGGCATATATTATAAACTCGTCCACAAATAATGGAAATCGGTCCGCGACCAGCTCGTTCGCATGCGGTTAAACGGCGGGTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct                    <  1:6116071/140‑1 (MQ=255)
                                                                                                                       tttAAAAAACAGGGCATATATTATAAAATCGTCGAAAAACAATGTAAATGGGTACGCCACCAGCTCGTGAGCATGCGGTTAAACGGGGGTTTTCCATATTTGACATTAAACGACGGTGATTACATGAAAAATAACGAGct                    <  1:3809130/140‑1 (MQ=255)
                                                                                                                       ttacAAAAACAGGGCCTACATTATAAAATCGTCCACAAACAATGCAAATCGGTCCGCGACCAGCTCTTGAGCATGCGGGTAAACGCCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct                    <  2:7346334/137‑1 (MQ=255)
                                                                                                                       ttacAAAAACAGGGCAGTGATTATAAATTCGTCGACAAACAATGTAAATCGGTACGCGACCCGGTGGTGAGCTTGGGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct                    <  1:396890/137‑1 (MQ=255)
                                                                                                                       ttaaaaaaaCAGGGCAGACATTATAAAATCGTCTAAAAACAATGTAAATCGTTACGCGACCAGCTCTTGAGAATGCGGTTAAAGGGCGGGTTTCAATATTTGACTTTAAACGACGGTGATTACATGAAAAATAACGAGct                    <  1:2264186/136‑1 (MQ=255)
                                                                                                                       tataaaaaaCAGGGCACAGTTAATAACATCGTCTAAAAACATTGGATATCGCTACGCCACAAGCTGGTGAGAATGGTGTAAAACGGCGGGTTTCATTTTTTGACAGAAAACGACGGTGATTACATGAAAAATAACGAGct                    <  2:6879068/136‑1 (MQ=255)
                                                                                                                       ctccAAAACCCGGCCAGATATTATAAAATCTTCGCAAAAGATTGCAATTCGGTACGCGACAAGCTCGTGAGCATGCGGGTAACCGGGGGTTTTCAATATTTCACAGTAAACGACGGTGATTACATGAAAAATAACGAGct                    <  1:3228198/137‑1 (MQ=255)
                                                                                                                              aaCAGGGCTGAGATTATAAATTCGTCTACAAAGAATGTAAATCGGTACGCTACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATAtt             <  2:3698672/140‑1 (MQ=255)
                                                                                                                              aaCAGGGCAGACATTATAAAAGCGACGACAAATAAGGGAAATCGGTACGCTACCAGCTCGTGAGCATGCGGGAAAACGGCGGTTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATAtt             <  2:5306111/140‑1 (MQ=255)
                                                                                                                                   ggCAGAGTTTATAAAATCGTCGACAAAGATTGGAAATCGGTACGCGACCAGCTGGTGAGCATGCGGGTAAACGGCGGGTTTCAATATTTGACCGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATATTAAGCa        <  2:5370624/140‑1 (MQ=255)
                                                                                                                                   ggCACAGATTAAAAAATCGTCCAAAAAGAATGCAAATGGGTACGCGACCAGCTCGTTAGAATGGGGTTAAACGGCGGGTTTCAATATTTGACTGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATATTAAGCa        <  1:5255639/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
GCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATATTAAGCATTGGTA  >  NZ_CP034943/986664‑986940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: