Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 131,088 C→A 100% S194Y (TCT→TAT)  fusA → elongation factor G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943131,0880CA99.5% 3199.4 / ‑1.0 907S194Y (TCT→TAT) fusAelongation factor G
Reads supporting (aligned to +/- strand):  ref base C (3/3);  new base A (457/444);  total (460/447)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 911 total aligned reads displayed.

AGGAACTTTAAGAGACCGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTCTGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAATACCTTGAGGGTGAAGAAATCACGATTGACGAG  >  NZ_CP034943/130951‑131224
                                                                                                                                         |                                                                                                                                        
    aCTTAAAGAGACCGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACAATGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGAtcc                                                                                                                                    >  2:7368495/1‑138 (MQ=255)
       ttAAGAGACCGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCgaa                                                                                                                                 >  2:322205/1‑140 (MQ=255)
       ttAAGAGACCGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCgaa                                                                                                                                 >  2:427610/1‑140 (MQ=255)
       ttAAGAGACCGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGAgaa                                                                                                                                 >  2:4636635/1‑140 (MQ=255)
       ttAAGAGACCGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAAAGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCgaa                                                                                                                                 >  2:7098964/1‑140 (MQ=255)
          agagACCGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCgaaaga                                                                                                                              <  2:2538279/140‑1 (MQ=255)
          agagACCGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCgaaaga                                                                                                                              <  2:6706116/140‑1 (MQ=255)
          agagACCGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGGTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCgaaaga                                                                                                                              <  1:4949076/140‑1 (MQ=255)
          agagACCGTCTTCAAGCAAACGCTCATGCAATTCAAGTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCgaaaga                                                                                                                              <  1:3283969/140‑1 (MQ=255)
              aCCGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATc                                                                                                                          >  2:4467870/1‑140 (MQ=255)
               ccGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATcc                                                                                                                         <  2:6957564/140‑1 (MQ=255)
               ccGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATcc                                                                                                                         <  1:5089056/140‑1 (MQ=255)
               ccGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATcc                                                                                                                         <  1:2819948/140‑1 (MQ=255)
                cGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATccg                                                                                                                        >  1:7133737/1‑139 (MQ=255)
                cGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATccc                                                                                                                        >  2:3464225/1‑140 (MQ=255)
                cGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTAGGATGCGAAAGAAATccc                                                                                                                        >  1:3972505/1‑140 (MQ=255)
                  tCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTg                                                                                                                      >  2:2461238/1‑140 (MQ=255)
                   cTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGATGACAACTTCGAAGGGATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGa                                                                                                                     <  2:3962800/140‑1 (MQ=255)
                    ttCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGaa                                                                                                                    <  2:5554274/140‑1 (MQ=255)
                     tCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAag                                                                                                                   <  1:1119541/140‑1 (MQ=255)
                     tCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAag                                                                                                                   <  2:1901774/140‑1 (MQ=255)
                     tCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAag                                                                                                                   <  2:5348281/140‑1 (MQ=255)
                     tCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAag                                                                                                                   <  2:4150164/140‑1 (MQ=255)
                           aaaCGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACa                                                                                                             <  2:4879389/140‑1 (MQ=255)
                           aaaCGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACa                                                                                                             <  1:1575854/140‑1 (MQ=255)
                            aaCGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAATTCACTGAAGAGTACaa                                                                                                            >  2:1530457/1‑140 (MQ=255)
                             aCGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACaaa                                                                                                           >  2:518179/1‑140 (MQ=255)
                                 tCATGCAATTCAATGGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTATACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCACTGAAGAGTACAAAGAGc                                                                                                       <  2:6532886/140‑1 (MQ=255)
                                    tGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAg                                                                                                    <  1:7240586/140‑1 (MQ=255)
                                         ttcaattGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGaa                                                                                               >  1:1979242/1‑140 (MQ=255)
                                         ttcaattGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGaa                                                                                               >  2:3097404/1‑140 (MQ=255)
                                          tcaatgGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATAGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAag                                                                                              <  1:6616358/140‑1 (MQ=255)
                                             attGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGc                                                                                           <  1:2499008/140‑1 (MQ=255)
                                                   gATCGGTGCTGACGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCa                                                                                     >  1:2982357/1‑140 (MQ=255)
                                                    ctCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTAGTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCaa                                                                                    <  1:7670805/139‑1 (MQ=255)
                                                    aTCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCaa                                                                                    <  1:7429909/140‑1 (MQ=255)
                                                        gTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGc                                                                                <  2:5661395/140‑1 (MQ=255)
                                                        gTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGc                                                                                <  1:78487/140‑1 (MQ=255)
                                                          gCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCt                                                                              <  1:4710043/140‑1 (MQ=255)
                                                              aGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCAtt                                                                          <  1:3157992/140‑1 (MQ=255)
                                                              aGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCAtt                                                                          <  1:3391584/140‑1 (MQ=255)
                                                               ggACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTg                                                                         <  2:7753340/140‑1 (MQ=255)
                                                                  cAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAg                                                                      <  2:6387333/140‑1 (MQ=255)
                                                                   aaCTTCGAAGGTCTCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAAAAGCCTCATTGAAGc                                                                     >  1:3717809/1‑140 (MQ=255)
                                                                   aaCTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGc                                                                     >  2:7611949/1‑140 (MQ=255)
                                                                   aaCTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGc                                                                     >  2:2304380/1‑140 (MQ=255)
                                                                   aaCTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGc                                                                     >  2:2167356/1‑140 (MQ=255)
                                                                    aCTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCt                                                                    <  2:5459429/140‑1 (MQ=255)
                                                                    aCTTCGAAGGTATCATCGACCGTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAAGCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCt                                                                    <  2:4064938/140‑1 (MQ=255)
                                                                     cTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGGGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTg                                                                   >  1:6138919/1‑140 (MQ=255)
                                                                          aaGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTtgt                                                              >  2:1312284/1‑140 (MQ=255)
                                                                               atcatcGACCTTGTAGAAAACGTTTATTATATCAACGAAGATGACCTCGGAACTCGTTATGATGAGAAAGAAATCACTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCt                                                         >  2:1682633/1‑140 (MQ=255)
                                                                               atcatcGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCt                                                         >  1:216913/1‑140 (MQ=255)
                                                                               atcatcGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCt                                                         <  2:1378150/140‑1 (MQ=255)
                                                                                tcatcGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCaa                                                                                    <  2:2669587/112‑1 (MQ=255)
                                                                                tcatcGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCtt                                                        >  1:2369650/1‑140 (MQ=255)
                                                                                tcatcGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCtt                                                        >  1:5492255/1‑140 (MQ=255)
                                                                                tcatcGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCtt                                                        >  2:2006227/1‑140 (MQ=255)
                                                                                tcatcGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGGTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCtt                                                        >  1:1908679/1‑140 (MQ=255)
                                                                                tcatcGACCTTGTAGAAAACGTTGCGTATTCTACGAAGATGACCTCGGAACTCGTTATGCTGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCGTAAGAGCTTAGCAACAGCCTCATTGAAGCTGTTTGTGAGCTtg                                                       >  1:429942/1‑140 (MQ=255)
                                                                                      aCCTTGTAGAGAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGaa                                                  <  1:4377325/140‑1 (MQ=255)
                                                                                      aCCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGaa                                                  <  2:5928278/140‑1 (MQ=255)
                                                                                      aCCTGGTAGAAAACGTTGCGTATTTCTACGAAGAGGACCTCCGAACTCGTTATGATGCGAAAGAAATCCCGGAACAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGaa                                                  <  2:4697336/140‑1 (MQ=255)
                                                                                        cTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGACGAGCTTCGCAACAGCCTCATTGACGCTGTTTGTGAGCTTGATGAaga                                                >  1:6005753/1‑140 (MQ=255)
                                                                                        cTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCGGTTTGTGAGCTTGATGAaga                                                >  1:4120409/1‑140 (MQ=255)
                                                                                          tGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGc                                              >  1:1006628/1‑140 (MQ=255)
                                                                                          tGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGc                                              >  2:6082125/1‑140 (MQ=255)
                                                                                             aGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTa                                           <  2:3359156/140‑1 (MQ=255)
                                                                                             aGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTa                                           <  1:5654107/140‑1 (MQ=255)
                                                                                             aGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTa                                           <  1:1411195/140‑1 (MQ=255)
                                                                                             aGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTa                                           <  1:1356118/140‑1 (MQ=255)
                                                                                             aGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTa                                           <  2:7487623/140‑1 (MQ=255)
                                                                                             aGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTa                                           <  1:6780234/140‑1 (MQ=255)
                                                                                             aGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTa                                           <  1:4550856/140‑1 (MQ=255)
                                                                                               aaaaCGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATg                                         <  2:3838572/140‑1 (MQ=255)
                                                                                                 aaCGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGa                                       >  1:4052428/1‑140 (MQ=255)
                                                                                                   cGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATa                                     <  2:1007352/140‑1 (MQ=255)
                                                                                                   cGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATa                                     <  2:6177343/140‑1 (MQ=255)
                                                                                                     ttGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATaaa                                   <  2:2858790/140‑1 (MQ=255)
                                                                                                     ttGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATaaa                                   <  2:724826/140‑1 (MQ=255)
                                                                                                      tGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAag                                                                                                                   <  1:4190722/59‑1 (MQ=255)
                                                                                                      tGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCt                                                                              <  2:5036883/96‑1 (MQ=255)
                                                                                                      tGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAATAGCTTATGGATAAAt                                  >  1:3859845/1‑140 (MQ=255)
                                                                                                      tGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTa                                           >  1:5799243/1‑131 (MQ=255)
                                                                                                      tGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTa                                           <  2:5799243/131‑1 (MQ=255)
                                                                                                      tGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAAt                                  >  1:7027516/1‑140 (MQ=255)
                                                                                                      tGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAAt                                  >  1:1742410/1‑140 (MQ=255)
                                                                                                           aTTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAATACCtt                             <  2:2937230/140‑1 (MQ=255)
                                                                                                             ttCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAATACCTTGa                           >  1:5306073/1‑140 (MQ=255)
                                                                                                             ttCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAATACCTTGa                           >  2:3646594/1‑140 (MQ=255)
                                                                                                                  cGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATCAATACCTTGAGGGTg                      >  2:6836765/1‑140 (MQ=255)
                                                                                                                  cGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAATACCTTGAGGGTg                      >  1:2685584/1‑140 (MQ=255)
                                                                                                                  cGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAATACCTTGAGGGTg                      >  1:360651/1‑140 (MQ=255)
                                                                                                                  cGAAGATGACCTCGGAACTCGTTATGAGGCGAAAGAAATCCCTGAAGAGTACCAAGAGCAAGCTGAAGTGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAATACCTTGAGGGTg                      >  2:6435452/1‑140 (MQ=255)
                                                                                                                         gACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAATACCTTGAGGGTGAAGAAAt               <  1:6391151/140‑1 (MQ=255)
                                                                                                                          aCCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGa                                       >  1:2416440/1‑115 (MQ=255)
                                                                                                                           ccTCGGACCTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGACCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAATACCTTGAGGGTGAAGAAATCa             <  2:1048608/140‑1 (MQ=255)
                                                                                                                              cGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTa                                           >  2:2765618/1‑107 (MQ=255)
                                                                                                                              cGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAATACCTTGAGGGTGAAGAAATCACGa          >  2:1183606/1‑140 (MQ=255)
                                                                                                                                      gTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAATACCTTGAGGGTGAAGAAATCACGATTGACGAg  <  1:886894/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
AGGAACTTTAAGAGACCGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTCTGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAATACCTTGAGGGTGAAGAAATCACGATTGACGAG  >  NZ_CP034943/130951‑131224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: