Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,097,474 G→T 15.0% E73* (GAA→TAA)  EO946_RS05675 → MarR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,097,4740GT15.0% 524.7 / 9.8 273E73* (GAA→TAA) EO946_RS05675MarR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (134/53);  new base T (3/45);  total (138/135)
Fisher's exact test for biased strand distribution p-value = 2.65e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 274 total aligned reads displayed.

CTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACGCCGGCCGGGAAAAAACTGCA  >  NZ_CP034943/1097336‑1097613
                                                                                                                                          |                                                                                                                                           
   aTTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGaaaa                                                                                                                                         >  1:5857597/1‑140 (MQ=255)
   aTTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTGGCTTGTGTTGGGGCCCATGACCCAATCAATGTCACGTCTATTGCAGaaaa                                                                                                                                         >  2:7616854/1‑140 (MQ=255)
    ttAAAGCCTTTTTGGCTGACGACTTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGaaaaa                                                                                                                                        >  1:1473194/1‑140 (MQ=255)
        aGCCTTTTTGGCTGACGAATTTAAGAAACTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTTTTTTGGGGACAATGAACCAATAAATGTCACGTCTATTGCAGAAAAAATGa                                                                                                                                    >  2:2264147/1‑140 (MQ=255)
        aGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGa                                                                                                                                    >  2:6085465/1‑140 (MQ=255)
         gCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGaa                                                                                                                                   <  1:567164/140‑1 (MQ=255)
         gCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTAGTGCAGAAAAAATGaa                                                                                                                                   <  2:2908460/140‑1 (MQ=255)
           cTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTGTTGTGTTGGGGACTATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATa                                                                                                                                 <  2:7067837/140‑1 (MQ=255)
           cTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATa                                                                                                                                 <  2:5377885/140‑1 (MQ=255)
             ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCAGGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                                               >  2:5264981/1‑140 (MQ=255)
             ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                                               >  1:1870841/1‑140 (MQ=255)
             ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                                               >  1:2311657/1‑140 (MQ=255)
             ttttGGCTGACGAATTGAAGAAGCTCGATGTAGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                                               >  1:4680325/1‑140 (MQ=255)
                  gcttaggAATTGAAGACGATCGTTGTCGAGCACGGGAGTAAATCTAATTTATCTATGACATAAATTCTTGTTTTTTTTTGTGTTGGGGAAAATGAACAAATCAATGTCAGGTTTTTTGCATAAAAAATGAATACGAaaaa                                                                                                                          <  2:3464194/134‑1 (MQ=255)
                      aCGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCg                                                                                                                      >  1:1676765/1‑140 (MQ=255)
                                   gCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCa                                                                                                         >  1:2178932/1‑140 (MQ=255)
                                    cTCGATGTCGAGGACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTTGGGACAATGAACCAATAAATGTCACGTCTATTGCATAAAAAATGAATACGACAAAAGCGCCTGTATCGAGCAt                                                                                                        >  2:4125682/1‑140 (MQ=255)
                                    cTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCAt                                                                                                        >  2:189803/1‑140 (MQ=255)
                                    cTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAAGCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCAt                                                                                                        >  2:461251/1‑140 (MQ=255)
                                         tttCGAGCACGGTATAAAAAATAAATTATCCATTACAGAAATTATTTTTCTTTTTGGTTTGGGGCACATTTAACAAATCAGTGTCAGTTATATTCAATAAAAAATGAAACCCACAACCGCGACTGTATCGCGCATCAGca                                                                                                   <  1:7010411/138‑1 (MQ=255)
                                         tGTCGCGCACGGTCTAAAACCTAAATTCTCTATCAAATAAATTCTTGTTCTTTCTTTTGTTGGGGACAATGAACCAATCATTTTCCCGTCTATGGCAGAAAAATTCAATACCACAAACGCGACTGTATCGCGCATCAGca                                                                                                   <  1:7249618/140‑1 (MQ=255)
                                          gTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGcac                                                                                                  >  1:7633557/1‑140 (MQ=255)
                                             gAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACaaa                                                                                               >  1:3238396/1‑140 (MQ=255)
                                             gAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACaaa                                                                                               >  1:4414808/1‑140 (MQ=255)
                                              aGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACaaaa                                                                                              >  1:1588865/1‑140 (MQ=255)
                                              aGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACaaaa                                                                                              >  1:1076163/1‑140 (MQ=255)
                                              aGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGGTCTTTCTGGTGTTGGGGACAATGAACCAATCAATGTCACGTCTCTTCCAGAAAAAATCAATACGACAAAAGCGACTGTATCGAGCATCAGCACaaaa                                                                                              >  2:1655281/1‑140 (MQ=255)
                                                   gggATTAAATCTAAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGAAAATGAACCAATCATTGTCACGTCTATTGCAGAAAAAATGAATACGACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCt                                                                                         <  2:5807354/140‑1 (MQ=255)
                                                     gAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATAAGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCg                                                                                       >  2:1526900/1‑140 (MQ=255)
                                                      ctaaaaTCTCAATTATCTATTACATAAATTAATTTTATTTATTTTTTTGGGTAAAATTAACAAATAATTTTCACGTATATTGCATAAAAAATTATTACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCgg                                                                                      <  2:4312429/137‑1 (MQ=255)
                                                           aTCTCAATTATCGTTGACATAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCgg                                                                                 <  2:5760262/140‑1 (MQ=255)
                                                           aTCTCAATTATCGATGACATAATTTCATGTTGTTTGTTGTGTTGGGGACAATCAACAAATCAATGTCACGTCTATTGCATAAAAAATGAATCCCACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCgg                                                                                 <  1:7089226/140‑1 (MQ=255)
                                                             ctcAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGt                                                                               >  2:5700126/1‑140 (MQ=255)
                                                             ctcAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTAGTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGt                                                                               >  2:7229122/1‑140 (MQ=255)
                                                             cacatttATCCATCAAACAAATTATTTTTCTTTTTTGTGTTGGGCAAAATCAACAAATCAATTTCCCTTATATGCCAAAAAAATTCAATACGACAACCGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGt                                                                               <  1:1158715/135‑1 (MQ=255)
                                                              tcAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGtt                                                                              >  1:1570217/1‑140 (MQ=255)
                                                              tcAATTATCCATGCAAGAAATTATTGTTCTTTGTTGTTTGGGGCACAATCAACCAAACATTGTCACGTCTTTTGCACAAAAAATGAAACCCACAACCGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGtt                                                                              <  1:6085465/140‑1 (MQ=255)
                                                                aaTTATCGTTGACAGAATTTCATGTTCTTTCTTGTGTTGGGGCCAATGAACAAATCATTGTCACGTCTATTGCAGAAAAAATGAATACGACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTc                                                                            <  2:434330/140‑1 (MQ=255)
                                                                aaTTATCGATGACAGAAATTCATGTTCTTTGTTGTGTGGGGTAAAATCAACCAATCATTGTCACGTCTATTGCATAAAAAATCAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTc                                                                            <  2:4133910/140‑1 (MQ=255)
                                                                aaTTATCGAGGAAATAAATTAATTTTCTTTCTTGTGTTGGGGAAAATGAACCAAAAAATGTCACGTCTATTGCAGAAAAAATTAATACGAAAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTc                                                                            <  2:1820053/140‑1 (MQ=255)
                                                                  ttATCTATGACACAAATTCATGTTCTTTTTTGTGTTGGTCAAAATTAACAAATCAATGTAACGTATTTTGAATAAAAAATTAAAACCACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCt                                                                          <  1:2514254/140‑1 (MQ=255)
                                                                  ttATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTTTGGCAGAAAAAATGAATACGACAACAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCt                                                                          <  2:402668/140‑1 (MQ=255)
                                                                  ttATCGATGAAATAATTTCATTTTCTTTCTTGTGTTGGGGAAAATGAACCAATAAATGTAACTTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCt                                                                          <  2:5248387/140‑1 (MQ=255)
                                                                  ttATCGATCACATAAATTCTTGTTATTTATTGTGTTGGTGACAATGAACCAATAATTGTCACGTCTTTTCCATAAAAAATGAATACTACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCt                                                                          <  2:3175730/140‑1 (MQ=255)
                                                                     tccttcaCATAATTTCTTTTTGTTTTTTTTGTTGGGCAAATTGACCAAAAAACTGTCACGTATATTCAATAAAAAATTAATACCAAAACCGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                                       <  1:2975834/134‑1 (MQ=255)
                                                                     tCGATGACATAATTTAATTTTGTTTGTTTTTTTGGGGACAATCAACAAATAAATGTCAGGTATATTGCATAAAAAATCAATACCACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                                       <  1:6270599/140‑1 (MQ=255)
                                                                     tCGATGACATAAATTCTTTTTCTTTGTTGTGTTGGGGAAAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATTAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                                       <  2:5518596/140‑1 (MQ=255)
                                                                     tCGATGACATAAATTATTGTTTTTTGTTGTGTTGGGGACAATGAACCAATCATTGTCACTTATATTGCATAAAAAATTAATACGAAAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                                       <  2:2518251/140‑1 (MQ=255)
                                                                     tCGATCACCCAATTTCATTTTTTTTATTGTGTTGGGCACAATGACCAAAACAATGTCACGTATATTGCATAAAAAATTAAACCCACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                                       <  1:5266347/140‑1 (MQ=255)
                                                                            cataaaTTCTTTTTCTTTCTTGTGTGGGTCAAAATCAACAAATAATTGTCAGTTTTTTTGAATAAAAAATTAATACCAAAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAaca                                                                <  1:6387955/137‑1 (MQ=255)
                                                                            cataaaTTATTGTTCTTTTTTGTTTTGGGAAAAATAAACAAATAAATGTCACTTATATTGCAAAAAAAATAAATACCAAAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAaca                                                                <  1:6795779/137‑1 (MQ=255)
                                                                            cataaaTTAATTTTATTTTTTGTGTTGGGCAAAATTAACAAAAAAATGTCACGTATATTGAACAAAAAATTAATACCCCAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAaca                                                                <  2:1991819/137‑1 (MQ=255)
                                                                                       tttctttgtTGTGTTGGGGACAATGAACAAATCATTGTCACGTCTATTGCAGAAAAAATTAATACCAAAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGa                                                     <  1:3688122/139‑1 (MQ=255)
                                                                                       tttctttctTGTTTTGGGGAAAAGGAACCAATCAAGGTCACGTATCTTGCATAAAAAATGAAGCCGACACACGCGACTGTCTCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACATAACACAGCTCAGCGa                                                     <  2:3919920/139‑1 (MQ=255)
                                                                                       tttctttctTGTGTTGGGCAAAATGAACAAATCAATGTCACGTCTCTTGCATAAAAAATCAATACGACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGa                                                     <  1:5676569/139‑1 (MQ=255)
                                                                                       gTTCTTTTTGTTGTTGGGTAAAATTAACAATTAATTGTCTCTTATATTCCATAAAAATTGAAAACCAAAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGa                                                     <  1:5139687/140‑1 (MQ=255)
                                                                                       gTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGa                                                     >  1:6701325/1‑140 (MQ=255)
                                                                                       gTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGa                                                     >  1:5452209/1‑140 (MQ=255)
                                                                                       gTTCTTTCTTGTGTTGGGCAAAATCAACAAATAAATGTAACGTCTTTTGCACAAAAAATTAATACCACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGa                                                     <  1:1645044/140‑1 (MQ=255)
                                                                                       gTTCTTTCTTGTGTTGGCCAAAATGAACAAAACAATGTAACTTCTATTGAACAAAAAATGAAAACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGa                                                     <  2:6538255/140‑1 (MQ=255)
                                                                                       gTTCTTTATTGTGTTGGGGACATTGAAACAATAAATGTCACGTATATTGCATAAAAAATGATTACCACAACAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGa                                                     <  2:1073920/140‑1 (MQ=255)
                                                                                        ttctttctTGTGTTTGGGACAATCAACCAAGCAATGTCACGTCTATTGCATAAAAAATGAACACCACAAAAGCGACTCTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGAt                                                    <  2:7659986/140‑1 (MQ=255)
                                                                                                 ttgtTGGGCACAATTAACCAATCATTGTCACGTATATTCCAGAAAAAATGAATACCACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAag                                           <  1:5608877/139‑1 (MQ=255)
                                                                                                  ttttGGGGACATTCAACCAAAAATTTGCAGTTCTTTTCCATAAAAAATAAATACCACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAagg                                          <  1:2264147/138‑1 (MQ=255)
                                                                                                  ttttGGGCAAAATTAACAAATAATTGTCACGTATTTTGAACAAAAAATCAATACCACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAagg                                          <  1:2488337/138‑1 (MQ=255)
                                                                                                  tgtgGGGCACCATCAACAAATCAAGGACACGTGTTTCCCACAAAAAATCAATACCACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAagg                                          <  1:2606869/140‑1 (MQ=255)
                                                                                                  tgtTGGGGACAATTAACAAATCAATGTCACTTATATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAagg                                          <  1:5400225/140‑1 (MQ=255)
                                                                                                  tgtTGGGCACAATGAACAAATCATTGTCACTTCTATTGAACAAAAAATTAATACCACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAagg                                          <  1:4061838/140‑1 (MQ=255)
                                                                                                    ttGGGGACAATGAACCAATCAATGTCAGGTTTATGGCCTAAAAAATTAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAaggag                                        <  1:7531048/140‑1 (MQ=255)
                                                                                                      ggggAAAATCAACCAATAAATGTCACTTCTTTTTCACAAAAAATGAATACGAAAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGt                                      <  2:5104881/140‑1 (MQ=255)
                                                                                                      ggctaCAATTAAACAATAACTGTCAGGTCTATTGAATAAAAAATTAATCATAAAACAGCGAATGTATCGAGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGt                                      <  1:7102897/136‑1 (MQ=255)
                                                                                                       gggACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTc                                     >  1:4567417/1‑140 (MQ=255)
                                                                                                            aaTGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAaca                                                                <  2:5635934/108‑1 (MQ=255)
                                                                                                            aaTGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACtt                                >  1:7164592/1‑140 (MQ=255)
                                                                                                            aaTGAACAAATCAATGTCACGTCTTTTGCATAAAAAATGAATACGACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACtt                                <  1:7752949/140‑1 (MQ=255)
                                                                                                              tGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTcc                              >  1:942980/1‑140 (MQ=255)
                                                                                                              tGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAAGTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGCGGGCTCCTTcc                              >  1:1492015/1‑140 (MQ=255)
                                                                                                                     atcaatGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCCGCGATAACAAAAAGGAGGACTACTTCCGATTGAc                       >  2:1323322/1‑140 (MQ=255)
                                                                                                                      tcaatGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATCACAAAAAGGAGGTCTACTTCCGATTGACg                      >  1:5700180/1‑140 (MQ=255)
                                                                                                                      tcaatGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACg                      >  1:2796466/1‑140 (MQ=255)
                                                                                                                      tcaatGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACg                      >  1:6586146/1‑140 (MQ=255)
                                                                                                                      tcaatCTCACGTCTATTGCATAAAAAATGAATACGACAAAAGCGACGGAATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACg                      >  1:1356763/1‑140 (MQ=255)
                                                                                                                          tGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACgccgg                  >  1:2399133/1‑140 (MQ=255)
                                                                                                                          tGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACgccgg                  >  1:1862271/1‑140 (MQ=255)
                                                                                                                          tGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACgccgg                  >  1:4278779/1‑140 (MQ=255)
                                                                                                                               cGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACGCCGGCCggg             >  1:3451349/1‑140 (MQ=255)
                                                                                                                               cGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACGCCGGCCggg             >  1:3997092/1‑140 (MQ=255)
                                                                                                                               cGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACGCCGGCCggg             >  1:3337245/1‑140 (MQ=255)
                                                                                                                               cGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACGCCGGCCggg             >  1:6155176/1‑140 (MQ=255)
                                                                                                                               cGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACGCCGGCCggg             >  1:3008792/1‑140 (MQ=255)
                                                                                                                               cGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACGCCGGACggg             >  2:1698090/1‑140 (MQ=255)
                                                                                                                               cGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGACGTCTACTTCCGATTGACGCCGGCCggg             >  2:6389293/1‑140 (MQ=255)
                                                                                                                                ttatATTGAATAAAAAATTAATACGAAAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACGCCGGCCGGGa            <  2:746875/137‑1 (MQ=255)
                                                                                                                                gtcaagggCAGAAAAAATCAAGACCACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACGCCGGCCGGGa            <  1:2742759/133‑1 (MQ=255)
                                                                                                                                gtatATTGAATAAAAAATGAATACCACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACGCCGGCCGGGa            <  1:7349140/137‑1 (MQ=255)
                                                                                                                                gacTATTGCAGAAAAAATTAATACGACAACAGCGACTGTATCGCGCATCAGCACACAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACGCCGGCCGGGa            <  2:577781/138‑1 (MQ=255)
                                                                                                                                    aTTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACGCCGGCCGGGaaaaa        >  2:585389/1‑140 (MQ=255)
                                                                                                                                    aTTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACGCCGGCCGGGaaaaa        >  2:7420395/1‑140 (MQ=255)
                                                                                                                                      tGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACGCCGGCCGGGAAAAAAc      >  1:7371936/1‑140 (MQ=255)
                                                                                                                                          gAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACGCCGGCCGGGAAAAAACTGca  >  1:6935572/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                           
CTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACGCCGGCCGGGAAAAAACTGCA  >  NZ_CP034943/1097336‑1097613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: