BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	1,167,036	G→T	18.5%	D192Y (GAC→TAC)	argB →	acetylglutamate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	1,167,036	0	G	T	18.5%	1042.5 / 224.1	464	D192Y (GAC→TAC)	argB	acetylglutamate kinase
Reads supporting (aligned to +/- strand): ref base G (191/187); new base T (45/41); total (236/228)
Fisher's exact test for biased strand distribution p-value = 8.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

Only 100 of 471 total aligned reads displayed.

GCTGTCTATGACGAGAGACTGCAAGACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTCAGCTTTAACGGATCAGGTGACTGAAGTCATGATCGTAAACGGTAAAGGA  >  NZ_CP034943/1166897‑1167173

gcTGTCTATGACGAGAGACTGCAAGACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTg                                                                                                                                           <  1:304354/140‑1 (MQ=255)

gcTGTCTATGACGAGAGACTGCAAGACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTg                                                                                                                                           <  1:1467330/140‑1 (MQ=255)

    tCTATGACGAGAGACTGCAAGACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGt                                                                                                                                       >  2:1794142/1‑140 (MQ=255)

       attaCGAGAGTCTGCAAGCAGCTTCATGTCAATGCTGATCTGGCTGCTTCCGCTGTAGCCGGAGCGCTTTAAGATGATAAGGTGCTGTTTGTCACTGATGAAGATGGGTTGATGAAAGATAAGCAGCGTCTTTACGTCCt                                                                                                                                    <  2:6034216/137‑1 (MQ=255)

       aTGACGAGAGACTGCAAGACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTTACGTCCt                                                                                                                                    <  2:7024939/140‑1 (MQ=255)

       aTGACGAGAGACTGCAAGACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTTACGTCCt                                                                                                                                    <  1:2480817/140‑1 (MQ=255)

       aTGACGAGAGACTGCAAGACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCt                                                                                                                                    <  2:6263727/140‑1 (MQ=255)

       aTGACGAGAGACTGCAAGACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCt                                                                                                                                    <  2:2222805/140‑1 (MQ=255)

          tcGAGAGACTGCTAGACGCTTAATGTGAATGCTGATCTGGCTGCTTCCGCTGTAGCCGGAGCGCTTGAAGCTGATAAGCTGTTGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTgtc                                                                                                                                 <  1:6791210/139‑3 (MQ=255)

          aCGAGAGACTGCAAGACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGAc                                                                                                                                 <  1:1053796/140‑1 (MQ=255)

              gagaCTGCAAGACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGTTGTTTGTCACTGATGTCGAGGGGATCTTGAAAGATAAGCAGCGTATTGACGTCCTGACCCCt                                                                                                                             >  2:1916949/1‑140 (MQ=255)

              gagaCTGCAAGACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCt                                                                                                                                    >  2:4782856/1‑133 (MQ=255)

              gagaCTGCAAGACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCt                                                                                                                             >  1:5196854/1‑140 (MQ=255)

              gagaCTGCAAGACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCt                                                                                                                             >  1:7133597/1‑140 (MQ=255)

              gagaCTGCAAGACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCt                                                                                                                             >  2:1670187/1‑140 (MQ=255)

              gagaCTGCAAGACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCt                                                                                                                             >  2:2632868/1‑140 (MQ=255)

              gagaCTGCAAGACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCt                                                                                                                             >  2:1094389/1‑140 (MQ=255)

              gagaCTGCAAGACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCt                                                                                                                             >  1:4596390/1‑140 (MQ=255)

              gagaCTGCAAGACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCt                                                                                                                             >  2:4264612/1‑140 (MQ=255)

              gagaCTGCAAGACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCt                                                                                                                             <  1:3212167/140‑1 (MQ=255)

              gagaCTGCAAGACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCt                                                                                                                             >  2:7640546/1‑140 (MQ=255)

              gagaCTGCAAGACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCt                                                                                                                             >  1:3567246/1‑140 (MQ=255)

              gagaCTGCAAGACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCt                                                                                                                             >  2:7437022/1‑140 (MQ=255)

                 aCTGCAAGACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTgag                                                                                                                          <  1:2063311/140‑1 (MQ=255)

                   tGCAAGACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTgagga                                                                                                                        <  1:4253867/140‑1 (MQ=255)

                   tGCAAGACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTgagga                                                                                                                        >  1:1577132/1‑140 (MQ=255)

                        gACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTTACGTCCTGACCCCTGAGGAGGTTc                                                                                                                   <  1:5875848/140‑1 (MQ=255)

                        gACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTc                                                                                                                   >  2:2726606/1‑140 (MQ=255)

                        gACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTc                                                                                                                   >  1:7763145/1‑140 (MQ=255)

                         aCGCTGAATGTGAATGCTGATCTGGCTGCGTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCa                                                                                                                  <  2:5506073/140‑1 (MQ=255)

                             tgaatgtgaatgCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTTACGTCCTGACCCCTGAGGAGGTTCAAATg                                                                                                              >  1:5805669/1‑140 (MQ=255)

                             tgaatgtgaatgCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTTACGTCCTGACCCCTGAGGAGGTTCAAATg                                                                                                              >  1:2431488/1‑140 (MQ=255)

                             tgaatgtgaatgCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATg                                                                                                              >  1:627199/1‑140 (MQ=255)

                             tgaatgtgaatgCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATg                                                                                                              >  1:6643614/1‑140 (MQ=255)

                             tgaatgtgaatgCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATg                                                                                                              >  1:7062122/1‑140 (MQ=255)

                             tgaatgtgaatgCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATg                                                                                                              >  2:1465680/1‑140 (MQ=255)

                             tgaatgtgaatgCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATg                                                                                                              >  2:1254818/1‑140 (MQ=255)

                                  gtgaatgCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGAt                                                                                                         <  2:6766155/140‑1 (MQ=255)

                                  gtgaatgCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGAt                                                                                                         <  2:1189874/140‑1 (MQ=255)

                                  gtgaatgCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGAt                                                                                                         <  2:5068180/140‑1 (MQ=255)

                                  gtgaatgCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGAt                                                                                                         <  1:4907016/140‑1 (MQ=255)

                                  gtgaatgCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGAt                                                                                                         <  2:2938727/140‑1 (MQ=255)

                                  gcgaatgCTGATCGGGCTGCGTCCGCAGTAGCCGGCGCGCTTGAAGCTGCTCAGCTGATGTTTGTCACTGAGGTCGAAGGGCTCATGAAAGATAAGCAGCGTCGGTACGTCCGGACCCCTGAGGAGGTTCAACTGCTGAt                                                                                                         <  2:2313968/138‑1 (MQ=255)

                                      atgCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTTACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAg                                                                                                     <  2:4045976/140‑1 (MQ=255)

                                       tgCGGATCTGGCTGCGTCCGCAGTAGCCGGAGCGCTTGAAGCTGAGAAGCTGATGTTTGTCACTGCTGTCCATGGGATCATGAAAGATACGCAGCGTCTGGACGTCCTGACCCCTGAGGAGGTTCCAATGCTGATCAAGc                                                                                                    <  2:642282/140‑1 (MQ=255)

                                         cTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTTACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAACCAg                                                                                                  >  2:4714693/1‑140 (MQ=255)

                                              cTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGt                                                                                             <  2:247289/140‑1 (MQ=255)

                                                 gctgctTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGAt                                                                                          >  1:5518799/1‑140 (MQ=255)

                                                 gctgctTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGAt                                                                                          <  2:578770/140‑1 (MQ=255)

                                                   tgctTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATca                                                                                        <  1:5729175/140‑1 (MQ=255)

                                                   tgcgTCCGCAGTAGCAGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATca                                                                                        <  1:4656246/140‑1 (MQ=255)

                                                     ctTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGCTCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATcaca                                                                                      <  2:818411/140‑1 (MQ=255)

                                                      ttCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTTACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAg                                                                                     >  1:6594070/1‑140 (MQ=255)

                                                      ttCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAg                                                                                     >  1:813295/1‑140 (MQ=255)

                                                      ttCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTATACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAg                                                                                     >  2:5662542/1‑140 (MQ=255)

                                                       tCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGATGAGGTTCAAATTATGATCAAGCAGGAAGTGATctcatg                                                                                    <  2:7287474/140‑3 (MQ=255)

                                                                gCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGgatgat                                                                           >  1:480026/1‑140 (MQ=255)

                                                                    gAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCg                                                                       <  1:3141927/140‑1 (MQ=255)

                                                                    gAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCg                                                                       <  1:4382552/140‑1 (MQ=255)

                                                                     aGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGa                                                                      >  1:2668871/1‑140 (MQ=255)

                                                                     aGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCGGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACGGGGGGGATGATTCCGa                                                                      >  1:3891536/1‑140 (MQ=255)

                                                                         cTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTTACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGt                                                                  <  2:3773011/140‑1 (MQ=255)

                                                                                   ataagctgatGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTTACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGcc                                                        >  2:6340655/1‑140 (MQ=255)

                                                                                   ataagctgatGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTTACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGcc                                                        >  2:1388158/1‑140 (MQ=255)

                                                                                   ataagctgatGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTCATCACAGCGGGGATGATTCCGAAAGTCAATTCAGcc                                                        >  1:4085939/1‑140 (MQ=255)

                                                                                   ataagctgatGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGATGATCAAGCAGGACGTGATCACAGGGGGCATGATTCCGAAAGTCAATTCAGcc                                                        >  1:5573262/1‑140 (MQ=255)

                                                                                       gctgatGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTTACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGt                                                    <  1:5317510/140‑1 (MQ=255)

                                                                                       gctgatGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTTACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGt                                                    <  1:3762237/140‑1 (MQ=255)

                                                                                       gctgatGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTTACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGt                                                    <  1:6151250/140‑1 (MQ=255)

                                                                                        ctgatGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTTACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTc                                                   <  1:2898034/140‑1 (MQ=255)

                                                                                        ctgatGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTc                                                   >  1:1119875/1‑140 (MQ=255)

                                                                                        ctgatGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTc                                                   >  2:7200646/1‑140 (MQ=255)

                                                                                                tGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTCAGCTTTaa                                           <  1:2255559/140‑1 (MQ=255)

                                                                                                tGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTCAGCTTTaa                                           >  1:2937113/1‑140 (MQ=255)

                                                                                                  tCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTTATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTGGTCAGCTTTAACg                                         >  2:4628120/1‑140 (MQ=255)

                                                                                                              aTGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGAGTCCGAAAGTCAATTCAGCCTTGTCAGCTTTAACGGATCAGGTGACt                             <  2:389313/140‑1 (MQ=255)

                                                                                                                   aTCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGc                                                                                                    >  2:2029976/1‑64 (MQ=255)

                                                                                                                     cATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTCAGCTTTAACGGATCAGGTGACTGAAGTCa                      <  2:3536581/140‑1 (MQ=255)

                                                                                                                     cATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTCAGCTTTAACGGATCAGGTGACTGAAGTCa                      <  2:6545895/140‑1 (MQ=255)

                                                                                                                     cATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCGGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTCAGCTTTAACGGATCAGGTGACTGAAGTCa                      <  2:2854404/140‑1 (MQ=255)

                                                                                                                       tGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTCAGCTTTACCGGATCAGGTGACTGAAGTCATg                    <  1:753404/140‑1 (MQ=255)

                                                                                                                       tGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTCAGCTTTAACGGATCAGGTGACTGAAGTCATg                    <  2:3428200/140‑1 (MQ=255)

                                                                                                                       tGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTCAGCTTTAACGGATCAGGTGACTGAAGTCATg                    <  2:317973/140‑1 (MQ=255)

                                                                                                                       tGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTCAGCTTTAACGGATCAGGTGACTGAAGTCATg                    >  1:7312497/1‑140 (MQ=255)

                                                                                                                             aTAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCCATTCAGCCTTGTCAGCTTTAACGGATCAGGTGACTGAAGTCATGATCGTa              >  2:5314486/1‑140 (MQ=255)

                                                                                                                             aTAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTCAGCTTTAACGGATCAGGTGACTGAAGTCATGATCGTa              <  1:1220328/140‑1 (MQ=255)

                                                                                                                             aTAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTCAGCTTTAACGGATCAGGTGACTGAAGTCATGATCGTa              <  1:1670187/140‑1 (MQ=255)

                                                                                                                             aTAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTCAGCTTTAACGGATCAGGTGACTGAAGTCATGATCGTa              >  2:3006104/1‑140 (MQ=255)

                                                                                                                               aaGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGACGGGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTCAGCTTTAACGGATCAGGTGACTGAAGTCATGATCGTaaa            >  2:3666237/1‑140 (MQ=255)

                                                                                                                                    gcGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTCAGCTTTAACGGATCAGGTGACTGAAGTCATGATCGTAAACGGTa       <  1:7212974/140‑1 (MQ=255)

                                                                                                                                    gcGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTCAGCTTTAACGGATCAGGTGACTGAAGTCATGATCGTAAACGGTa       <  1:959580/140‑1 (MQ=255)

                                                                                                                                    gcGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTCAGCTTTAACGGATCAGGTGACTGAAGTCATGATCGTAAACGGTa       <  1:6437162/140‑1 (MQ=255)

                                                                                                                                    gcGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTCAGCTTTAACGGATCAGGTGACTGAAGTCATGATCGTAAACGGTa       <  1:4218285/140‑1 (MQ=255)

                                                                                                                                    gcGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTCAGCTTTAACGGATCAGGTGACTGAAGTCATGATCGTAAACGGTa       <  1:7640546/140‑1 (MQ=255)

                                                                                                                                    gcGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTCAGCTTTAACGGATCAGGTGACTGAAGTCATGATCGTAAACGGTa       <  1:3281686/140‑1 (MQ=255)

                                                                                                                                     cGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTCAGCTTTAACGGATCAGGTGACTGAAGTCATGATCGTAAACGGTaa      <  1:1815287/140‑1 (MQ=255)

                                                                                                                                       tCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGt                                                                  <  1:3587655/78‑1 (MQ=255)

                                                                                                                                       tCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTCAGCTTTAACGGATCAGGTGACTGAAGTCATGATCGTAAACGGTAAAg    >  2:5827729/1‑140 (MQ=255)

                                                                                                                                       tCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTCAGCTTTAACGGATCAGGTGACTGAAGTCATGATCGTAAACGGTAAAg    >  1:1927795/1‑140 (MQ=255)

                                                                                                                                       tCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTCAGCTTTAACGGATCAGGTGACTGAAGTCATGATCGTAAACGGTAAAg    >  2:4917269/1‑140 (MQ=255)

GCTGTCTATGACGAGAGACTGCAAGACGCTGAATGTGAATGCTGATCTGGCTGCTTCCGCAGTAGCCGGAGCGCTTGAAGCTGATAAGCTGATGTTTGTCACTGATGTCGATGGGATCATGAAAGATAAGCAGCGTCTTGACGTCCTGACCCCTGAGGAGGTTCAAATGCTGATCAAGCAGGAAGTGATCACAGGGGGGATGATTCCGAAAGTCAATTCAGCCTTGTCAGCTTTAACGGATCAGGTGACTGAAGTCATGATCGTAAACGGTAAAGGA  >  NZ_CP034943/1166897‑1167173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`