Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,279,924 C→A 5.9% I50I (ATC→ATA EO946_RS06645 → altronate dehydratase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,279,9240CA 5.9% 1348.7 / 8.8 564I50I (ATC→ATAEO946_RS06645altronate dehydratase family protein
Reads supporting (aligned to +/- strand):  ref base C (215/289);  new base A (2/58);  total (217/347)
Fisher's exact test for biased strand distribution p-value = 3.08e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 567 total aligned reads displayed.

GTATAAAGATTCATAAACAAGATAATGTCCTTCTTGCTCTGCGAGATATCGAGAAAGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACATACAGCTATACACCGAGAT  >  NZ_CP034943/1279785‑1280063
                                                                                                                                           |                                                                                                                                           
   taAAGATTCATAAACAAGATAATGTCCTTCTTGCTCTGCGAGATATCGAGAAAGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGGGGCCACAAGATTGCGCTTCAGCCTATCaaa                                                                                                                                          >  1:2545300/1‑140 (MQ=255)
   taAAGATTCATAAACAAGATAATGTCCTTCTTGCTCTGCGAGATATCGAGAAAGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCaaa                                                                                                                                          <  2:5878407/140‑1 (MQ=255)
     aaGATTCATAAACAAGATAATGTCCTTCTTGCTCTGCGAGATATCGAGAAAGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCaaata                                                                                                                                        >  2:1913069/1‑138 (MQ=255)
           cATAAACAATATAATGTCCTTCTTGCTCTGCTAGATATCGAGAAAGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGa                                                                                                                                  <  2:1052516/140‑1 (MQ=255)
           cATAAACAAGATAATGTCCTTCTTGCTCTGCGAGATATCGAGAAAGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGGGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGa                                                                                                                                  <  2:2545300/140‑1 (MQ=255)
           cATAAACAAGATAATGTCCTTCTTGCTCTGCGAGATATCGAGAAAGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGa                                                                                                                                  >  2:5927964/1‑140 (MQ=255)
               aaCAAGATAATGTCCTTGTTGCGCGCCGAGATATCGAGAAAGGAGAGCGCCTGAATGCGGATGGCTTGTCGATTGACGTCAAGGATGATATAAAAAGAGGCCAAAACATGGCGGTTCAGCCTATCAAAGAAAACGACAGc                                                                                                                              <  1:6045728/140‑1 (MQ=255)
               aaCAAGATAATGTCCTTCTTGCTCTGCGAGATATCGAGAAAGGAGAGCGGCTGAATGCGGATGGAATGTCGATTGAAGTCAAGGTTGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGc                                                                                                                              <  2:6650010/140‑1 (MQ=255)
                  aaGATAATGTCCTTCTTGCTCTGCGAGATATCGAGAAAGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGcatc                                                                                                                           >  2:2791412/1‑140 (MQ=255)
                   tgATATTGTCCTTCTTGCTCTGCGAGATATCGAGAAAGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAACGAAAACGACAGcatca                                                                                                                          <  2:5468433/139‑1 (MQ=255)
                   aGATAATGTCCTTCTTGCTCTGCGAGATATCGAGAAAGGAGAGCGGCTGACTGCGGAGGGCATGTCGATTTAAGTAAAGGATGATATAAAAAGAGGCCACAAGTTTGCGGTTCAGCATATCAAAGAACACGACAGcatca                                                                                                                          <  2:3927683/140‑1 (MQ=255)
                         tGTCCTTCTTGCTCTGCGAGATATCGAGAAAGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATCTAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAAt                                                                                                                    >  1:1061656/1‑140 (MQ=255)
                         tGTCCTTCTTGCTCTGCGAGATATCGAGAAAGGAGAGCCGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGCTTGCGCTTCAGCCTAGCAACGAAAACGACAGCATCATCAAAt                                                                                                                    >  2:4595728/1‑140 (MQ=255)
                           tCCTTCTTGCTCTGCGAGATATCGAGAAAGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATAc                                                                                                                  >  2:3561576/1‑140 (MQ=255)
                              ttcttGCTCTGCGAGATATCGAGAAAGTAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACggg                                                                                                               >  2:1495317/1‑140 (MQ=255)
                               tcttGCTCTGCGAGATATCGAGAAAGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGt                                                                                                              >  1:3350560/1‑140 (MQ=255)
                                cttGCTCTGCGAGATATCGAGAAAGGAGTGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGtt                                                                                                             >  2:6902696/1‑140 (MQ=255)
                                 ttGCTCTGCGAGATATCGAGAAAGGAGAGCGGCTGACTGCGGATGGCATGTCGATTGAAGTCAAGTATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGttt                                                                                                            <  2:3192900/140‑1 (MQ=255)
                                 ttGCTCTGCGAGATATCGAGAAAGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCACGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGttt                                                                                                            <  2:493633/140‑1 (MQ=255)
                                 ttGCTCTGCGAGATATCGAGAAAGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATTATATAAAAAGAGGCCACAAGATTGCGCTTCAGCATATCAAAGAAAACGACAGCATCATCAAATACGGGttt                                                                                                            <  2:2475380/140‑1 (MQ=255)
                                       tGCGAGATATCGAGAAAGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATc                                                                                                      >  2:5777188/1‑140 (MQ=255)
                                       tGCGAGATATCGAGAAAGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATc                                                                                                      >  1:6959458/1‑140 (MQ=255)
                                       tGCGAGATATCGAGAAAGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATc                                                                                                      >  2:3267473/1‑140 (MQ=255)
                                         cGAGATATCGAGAAAGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTGTCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCgg                                                                                                    >  1:4414787/1‑140 (MQ=255)
                                           atatatCGAGAAAGGAGAGCGGCTGAATGCGGAGGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGCCCACAAGTTGGCGCTTCAGCCTATCAAACAACACGACAGCATCATCAAATACGGGTTTCCAATCGGgc                                                                                                  <  1:569386/138‑1 (MQ=255)
                                            gaTATCGAGAAAGGACAGCTGCTGAATGCGGATGGAATGTCTATTGAATTCAAGTATGATATAAAAAGAGGCCAAAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGgca                                                                                                 <  2:7249189/140‑1 (MQ=255)
                                              tatCTAGAAAGGAGAGCGGCTGATTGGGTATGGCATTTCGTTTGAAGTCAAGGATGTTATAAAAAGAGGCCACAAGTTTGCGCTTCAGCCTATAAAACAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGgcatg                                                                                               <  1:7348491/140‑1 (MQ=255)
                                              tatCGAGAATGGAGAGGGGCTGATTGCGTATGGCTTGTCTATTGATTTAATGGATGTTATAAAAAGTGGCCACAAGTTTGCGCTTCAGCCTATAAAACAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGgcatg                                                                                               <  1:803610/140‑1 (MQ=255)
                                              tatCGAGAAAGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGTATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGgcatg                                                                                               <  2:1306885/140‑1 (MQ=255)
                                              tatCGAGAAAGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAATAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGtcatg                                                                                               >  2:7370568/1‑140 (MQ=255)
                                              tatCGAGAAAGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGgcatg                                                                                               <  2:798685/140‑1 (MQ=255)
                                               atCGAGAAAGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGgcatgc                                                                                              >  2:6085166/1‑140 (MQ=255)
                                                 cGAGAAAGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGgcatgcat                                                                                            >  2:5645058/1‑140 (MQ=255)
                                                  gagaACGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTACAGCCTCTCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATc                                                                                           <  1:1682386/140‑1 (MQ=255)
                                                  gagaAAGGAGAGCGGCTGAATGCGGATGGCATGTCTATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGGTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATc                                                                                           <  2:5025787/140‑1 (MQ=255)
                                                   agaAAGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATca                                                                                          <  2:4635068/140‑1 (MQ=255)
                                                   agaAAGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATca                                                                                          <  1:7538614/140‑1 (MQ=255)
                                                   agaAAGGAGAGCGGCTGAATGAGTATTTAAATTAGATTGAAGTCAACGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCAAATAGGGCATGCATca                                                                                          >  2:74758/1‑140 (MQ=255)
                                                     aaaGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATcaca                                                                                        <  2:4503270/140‑1 (MQ=255)
                                                              cGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCACTCGGGCATGCATCACAAGATATatc                                                                               >  1:466868/1‑140 (MQ=255)
                                                              cGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATatc                                                                               >  1:6272178/1‑140 (MQ=255)
                                                                     aTGGGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGga                                                                        <  2:5539980/140‑1 (MQ=255)
                                                                     aTGCGGTTGGAATTTCGTTTGAAGTCAAGTATGATATAAAAAGAGGCAACAAGTTTGAGCTTCAGCCTATCAAAGAAAACGACAGCCTCATCAACTACGGGTTTACAAGCGGGCATGCATCACAAGATATATCGATCGga                                                                        <  2:5198100/140‑1 (MQ=255)
                                                                     aTGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGga                                                                        <  2:5269697/140‑1 (MQ=255)
                                                                     aTGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGga                                                                        <  2:4797191/140‑1 (MQ=255)
                                                                     aTGCGCATGCCATGTCCATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAACACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGga                                                                        <  1:2269156/140‑1 (MQ=255)
                                                                         ggATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGc                                                                    <  2:4189906/140‑1 (MQ=255)
                                                                           aTGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCat                                                                  >  1:6745125/1‑140 (MQ=255)
                                                                           aTGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCat                                                                  >  2:4298420/1‑140 (MQ=255)
                                                                              gCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTACAGCCTATCAAACAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATAtt                                                               <  1:6445403/140‑1 (MQ=255)
                                                                                 tGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCGTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCAt                                                            <  2:3831725/140‑1 (MQ=255)
                                                                                       ttGAAGTAAAGTATTATATAAAAAGAGGCCAAAAGATTGCGCTTAAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCat                                                      <  1:2643742/140‑1 (MQ=255)
                                                                                        tGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCata                                                     >  2:2344840/1‑140 (MQ=255)
                                                                                            ctCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATAc                                                 <  1:4595564/139‑1 (MQ=255)
                                                                                             tCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACa                                                >  2:6065609/1‑140 (MQ=255)
                                                                                                   atgatATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAaaaca                                          <  2:7002747/140‑1 (MQ=255)
                                                                                                    tgatATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAAAACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAaaacaa                                         >  1:3379140/1‑140 (MQ=255)
                                                                                                     tatatAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAaaacaaa                                        <  2:4050191/139‑1 (MQ=255)
                                                                                                      atatAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGagag                                                                     >  1:3695808/1‑110 (MQ=255)
                                                                                                       tataAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAAcc                                      >  1:2147713/1‑140 (MQ=255)
                                                                                                            aaaGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACTGGTTTCCAATCGGGCATGCATCACAAGATCTATCGATCGGAGAGCCTATTCATGTCCATCATACAAAAACAAACCTGTCt                                 >  1:4946740/1‑140 (MQ=255)
                                                                                                            aaaGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCt                                 >  1:3202346/1‑140 (MQ=255)
                                                                                                            aaaGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCt                                 >  1:6572721/1‑140 (MQ=255)
                                                                                                                agGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGata                             <  2:3379140/140‑1 (MQ=255)
                                                                                                                 ggCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGatat                            >  2:6194382/1‑140 (MQ=255)
                                                                                                                  gCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATc                           >  1:5296479/1‑140 (MQ=255)
                                                                                                                   ccACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATcc                          <  2:3674253/140‑1 (MQ=255)
                                                                                                                   ccACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATcc                          <  1:2344840/140‑1 (MQ=255)
                                                                                                                   ccACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATcc                          <  1:1895126/140‑1 (MQ=255)
                                                                                                                     acaAGATTGCGCTTCAGCCTATAAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCaa                        <  1:2078810/140‑1 (MQ=255)
                                                                                                                        atatggcgcTTCAGCCTCTCAAAGAAAACGACAGCATCATCAAAGACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACa                     <  1:4072554/135‑1 (MQ=255)
                                                                                                                        agtttGCGCTTCAGCCTATAAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACa                     <  1:2832847/137‑1 (MQ=255)
                                                                                                                        aGATTGCGCTTCAGCCTATCAAATAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACa                     <  2:296349/140‑1 (MQ=255)
                                                                                                                        aGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACa                     <  1:3093564/140‑1 (MQ=255)
                                                                                                                        aGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACa                     <  2:5091595/140‑1 (MQ=255)
                                                                                                                        aGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACa                     <  2:6283319/140‑1 (MQ=255)
                                                                                                                        aGATTGCGCTTCAGACTATAAAATAACACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACa                     <  1:1836579/140‑1 (MQ=255)
                                                                                                                        aGATTGCGATTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACa                     <  2:6505770/140‑1 (MQ=255)
                                                                                                                        aGATGGCGCTTCAGCCTATAAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACa                     <  1:1236578/140‑1 (MQ=255)
                                                                                                                          aTTGCGTTTCAGACTATAAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACATa                   <  1:5923588/140‑1 (MQ=255)
                                                                                                                          aTTGCGGTTCAGCCTATCAAAGAAAAAGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACATa                   <  1:3861632/140‑1 (MQ=255)
                                                                                                                          aTTGCGCTTCAGCCTATCAATTAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACATa                   <  2:640618/140‑1 (MQ=255)
                                                                                                                          aTTGCGCTTCAGCCTATCAAAGAACACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACATa                   <  1:3500937/140‑1 (MQ=255)
                                                                                                                          aTTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAACCCTGTCTGATATCCAAACATa                   <  2:672138/140‑1 (MQ=255)
                                                                                                                          aTTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACCTa                   <  2:1728466/140‑1 (MQ=255)
                                                                                                                          aTTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACATa                   <  1:1502173/140‑1 (MQ=255)
                                                                                                                          aTTGCGCTTCAGCCTATAAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACATa                   <  1:7662027/140‑1 (MQ=255)
                                                                                                                          aTTGCGCTTCAGCCTATAAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACATa                   <  1:4170069/140‑1 (MQ=255)
                                                                                                                          aTTGCGCTTCAGCCTATAAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACATa                   <  2:7600061/140‑1 (MQ=255)
                                                                                                                          aTTGCGCTTCAGCATATAAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACATa                   <  1:5498399/140‑1 (MQ=255)
                                                                                                                            tGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGa                               <  2:1832991/126‑1 (MQ=255)
                                                                                                                              cgcTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACATACAGc               >  1:5124360/1‑140 (MQ=255)
                                                                                                                              cgcTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACATACAGc               >  1:483459/1‑140 (MQ=255)
                                                                                                                               gcTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATAACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACATACAGCt              >  2:5979606/1‑140 (MQ=255)
                                                                                                                               gcTTAAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACATACAGCt              <  1:6085166/140‑1 (MQ=255)
                                                                                                                                 ttCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACATACAGCtat            >  1:5072680/1‑140 (MQ=255)
                                                                                                                                   cAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGga                                                                        <  1:3244033/78‑1 (MQ=255)
                                                                                                                                        tATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACATACAGCTATACACCga     >  2:2641615/1‑140 (MQ=255)
                                                                                                                                        tATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACATACAGCTATACACCga     >  2:3096293/1‑140 (MQ=255)
                                                                                                                                          tCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACATACAGCTATACACCgaga   >  2:916050/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GTATAAAGATTCATAAACAAGATAATGTCCTTCTTGCTCTGCGAGATATCGAGAAAGGAGAGCGGCTGAATGCGGATGGCATGTCGATTGAAGTCAAGGATGATATAAAAAGAGGCCACAAGATTGCGCTTCAGCCTATCAAAGAAAACGACAGCATCATCAAATACGGGTTTCCAATCGGGCATGCATCACAAGATATATCGATCGGAGAGCATATTCATGTCCATAATACAAAAACAAACCTGTCTGATATCCAAACATACAGCTATACACCGAGAT  >  NZ_CP034943/1279785‑1280063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: